• The Plant Pathology Journal
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• v.38 no.4
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• pp.334-344
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• 2022

Bacterial wilt is a re-emerging disease on dry bean and can affect many other crop species within the Fabaceae. The causal agent, Curtobacterium flaccumfaciens pv. flaccumfaciens (CFF), is a small, Gram-positive, rod-shaped bacterium that is seed-transmitted. Infections in the host become systemic, leading to wilting and economic loss. Clean seed programs and bactericidal seed treatments are two critical management tools. This study characterizes the efficacies of five bactericidal chemicals against CFF. It was hypothesized that this bacterium was capable of forming biofilms, and that the cells within biofilms would be more tolerant to bactericidal treatments. The minimum biocide eradication concentration assay protocol was used to grow CFF biofilms, expose the biofilms to bactericides, and enumerate survivors compared to a non-treated control (water). Streptomycin and oxysilver bisulfate had EC95 values at the lowest concentrations and are likely the best candidates for seed treatment products for controlling seed-borne bacterial wilt of bean. The results showed that CFF formed biofilms during at least two phases of the bacterial wilt disease cycle, and the biofilms were much more difficult to eradicate than their planktonic counterparts. Overall, biofilm formation by CFF is an important part of the bacterial wilt disease cycle in dry edible bean and antibiofilm bactericides such as streptomycin and oxysilver bisulfate may be best suited for use in disease management.

• Journal of Korean Medicine for Obesity Research
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• v.21 no.2
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• pp.69-79
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• 2021

Objectives: This study aimed to compare the anti-obesity effects of raw and pickled garlic in vitro in 3T3-L1 preadipocytes. Methods: The pickled garlic samples comprised the following: garlic aged in vinegar (VG), garlic aged in soy sauce, and vinegar (1:1, v/v) (PG) and raw garlic (RG) as control. Hexane, butanol, and distilled water were used to prepare the fractions. The pancreatic lipase inhibitory activity was used as a measure of anti-obesity effects of the extracts. The lipid droplet accumulation and triglyceride content in the 3T3-L1 cells were measured using Oil red O staining and triglyceride assay kits, respectively. The adipogenesis related protein expression levels were analyzed using the kits and the western blot method. Results: The pancreatic lipase inhibitory activity of the garlic extracts (VG, PG, RG) was the highest in the butanol fraction, and the inhibitory effect was the highest in RG, followed by PG and VG. All garlic butanol extracts suppressed triglyceride accumulation in differentiated adipocytes (P<0.05) through the activation of cyclic adenosine monophosphate (AMP), AMP-activated protein kinase, carnitine palmitoyl transferase-1, and the inhibition of fatty acid synthase. Raw garlic extracts significantly inhibited the expression of proteins involved in adipogenesis as compared to pickled garlic. Conclusions: Raw garlic has the potential to be an effective natural material for reducing obesity compared to pickled garlic with vinegar or soy sauce.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.55 no.5
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• pp.688-696
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• 2022

Obesity could cause immune-physiological disorders in fish. Yet, little is known about the impact of obesity on stress and histological responses. This study aimed to determine histological and physiological changes in and vulnerability of overfeeding-induced obese rainbow trout Oncorhynchus mykiss exposed to stress condition. Control, intermediate and overfed groups were fed at 1.5, 2.5 and 3.09% of their body weight per day, respectively, for eight weeks. Weight gain, body mass index, hepatosomatic index and serological parameters, and histology of liver were measured in five fish from each group at week 0, 2, 4, and 8. At week 8, 20 fish from each group were exposed to heat stress by increasing water temperature at a rate of 3℃ per day from 15 to 25℃ and maintaining the final temperature for 10 days. Overall, overfed fish showed significantly higher weight gain, body mass index, and serological parameters than those of fish in the other groups. Fish in the overfed and intermediate groups displayed multifocal infiltration of inflammatory cells in hepatic parenchyma. Mortality rate and serological parameters of fish in the overfed group exposed to heat stress were significantly higher than those of fish in the other groups, indicating increased vulnerability to environmental stress.

• Journal of the Society of Naval Architects of Korea
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• v.59 no.5
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• pp.280-287
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• 2022

An open rack vaporizer is a facility that vaporizes liquefied natural gas using sea water. When a vaporization efficiency of the open rack vaporizer decreases, liquefied natural gas can leak, which can cause great damage to the facility. Operators have to monitor the operation state of the facility in real-time to prevent the accident. However, operators have visited the site and have checked the state by looking at the value of sensors installed in the open rack vaporizer through indicators. For the safe operation of the open rack vaporizer, a monitoring system is needed to monitor the operation state of the open rack vaporizer in real-time without the need for operators to visit the site. In this paper, we developed a long term evolution based monitoring system to monitor the operation state of the open rack vaporizer. The developed system can monitor the real-time operation state of the open rack vaporizer at a control center far from the facility. For the system development, data transmission infrastructure using long term evolution was built. Afterwards a software was developed to monitor the operation state of the open rack vaporizer in real-time using the transmitted data. Finally, performance evaluation was conducted to confirm that the developed system operated successfully without data transmission delay or data missing.

• Journal of The Korean Society of Agricultural Engineers
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• v.64 no.3
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• pp.33-43
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• 2022

South Korea's agricultural nitrogen balance and phosphorus balance rank first and second, respectively, among OECD countries, and proper nutrient management is required to preserve the water quality of rivers and lakes. This study evaluates the effects of furrow mulching on the reduction of non-point source pollution (NPS) load from a sloped upland. The study site was Wanju-gun, Jeollabuk-do, and the survey period was from 2018 to 2019. The slope of the testbed was 13%, and the soil type was sandy loam. The cropping system consisted of maize-autumn Chinese cabbage rotation. The testbed was composed of bare soil (bare), control (Cont.), furrow vegetation mulching (FVM), and furrow nonwoven fabric mulching (FFM) plots. Runoff was collected for each rainfall event with a 1/100 sampler, and the NPS load was calculated by measuring the concentrations of SS, T-N, and T-P. The NPS load was then analyzed for the entire monitoring and crop cultivation periods. During the monitoring period, the effect of reducing the NPS load was 1.5%~44.5% for FVM and 13.1%~55.2% for FFM. During the crop cultivation period, it was 1.2%~80.5% for FVM and 27.0%~65.1% for FFM, indicating that FFM was more effective than FVM. As the NPS load was fairly high during the crop conversion period, an appropriate management method needs to be implemented during this period.

• The Journal of the Acoustical Society of Korea
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• v.41 no.5
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• pp.501-506
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• 2022

Echogenic liposome contains both liquid and gas inside the shell. In ultrasound mediated drug delivery, sonoporation, these new microbubbles can be an attractive drug carrier since they can be loaded water soluble drugs and drug molecules can be unloaded at the specific location with ultrasound sonication. In this paper, the structure of the echogenic liposome was confirmed with EF-TEM and the positive effect of sonoporation with echogenic liposome was comparatively evaluated on MDA-MB-231 cells which is a type of breast cancer cell with Doxorubicin. Control group (Group 1), Doxorubicin only (Group 2), sonoporation with Doxorubicin and hollow microbubbles (Group 3), sonoporation with Doxorubicin loaded echogenic liposome (Group 4) were classified and experiments were conducted. According to the results, Group 4 is at least 1.4 times better in inducing necrosis of cancer cells. Therefore, we conclude echogenic liposome could be one of the most useful form of microbubbles in sonoporation.

• International Journal of Internet, Broadcasting and Communication
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• v.13 no.2
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• pp.145-155
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• 2021

With the recent rapid improvement in the standards of life and westernization of dietary lifestyles, the consumption of high-calorie diets such as high-fat and high-protein red meat and instant foods has increased, while less vegetables containing dietary fiber are consumed. In addition to that, stress, erroneous dietary behaviors, and contaminated environments are linked to the risk of developing ulcerative colitis, which is on the rise. Another cause of ulcerative colitis is that involve laxative abuse, including repeated, frequent use of laxatives, and include such conditions as deteriorated bowel function, irritable bowel syndrome, diarrhea, intestinal inflammation, etc. The present study aimed to investigate the comparative evaluation of pharmacological efficacy between sulfasalazine alone and combination with herbal medicine on dextran sodium sulfate (DSS)-induced UC in mice. Balb/c mice received 5% DSS in drinking water for 7 days to induce colitis. Animals were divided into five groups (n = 9): group I-normal group, group II-DSS control group, group III-DSS + sulfasalazine (30 mg/kg), group IV-DSS + sulfasalazine (60 mg/kg), group V-DSS + sulfasalazine (30 mg/kg) + Radish Extract mixture (30 mg /kg) (SRE). DSS-treated mice developed symptoms similar to those of human UC, such as severe bloody diarrhea and weight loss. SRE supplementation, as well as sulfasalazine, suppressed colonic length and mucosal inflammatory infiltration. In addition, SRE treatment significantly reduced the expression of pro-inflammatory signaling molecules through suppression both mitogen-activated protein kinases (MAPK) and nuclear factor-kappa B (NF-κB) signaling pathways, and prevented the apoptosis of colon. Moreover, SRE administration significantly led to the up-regulation of antioxidant enzyme including SOD and Catalase. This is the first report that Radish extract mixture combined with sulfasalazine protects against experimental UC via the inhibition of both inflammation and apoptosis, very similar to the standard-of-care sulfasalazine.

• Korean journal of applied entomology
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• v.60 no.2
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• pp.229-234
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• 2021

This study investigated spray patterns and coverage generated by three types of commercial nozzles for spraying pesticides with Unmanned Aerial Vehicles (UAVs) using a multi-copter. Flufenoxuron+metaflumizone SC and bifenthrin EC were sprayed. The falling particles of the spraying agent were measured using WSP (Water and oil Sensitive Paper) and the coverage was determined. The results showed that the uniformity of falling particles was different according to the difference in wind strength, and there was no difference for different formulations. The injection amount for each nozzle was found to be different from the official information provided by the manufacturers. These results could be used to establish guidelines for the control of UAVs and pesticide registration testing.

• Microbiology and Biotechnology Letters
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• v.49 no.2
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• pp.157-166
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• 2021

The probiotic market is constantly continuing to grow, concomitantly with a widening in the range and diversity of probiotic products. Probiotics are defined as live microorganisms that provide a benefit to the host when consumed at a proper dose; the viability of a probiotic is therefore of crucial importance for its efficacy. Many products undergo lyophilization for maintaining their shelf-life. Unfortunately, this procedure may damage the integrity of the cells due to stress conditions during both the freezing and (vacuum-) drying process, thereby impacting their functionality. We propose a lysine-based mixture for rehydration of freeze-dried probiotics for improving their viability during in vitro simulated gastric and duodenum stress conditions. Measurement of the zeta potential served as an indicator of cell integrity and efficacy of this mixture, while functionality was estimated by adhesion to a human enterocyte-like Caco-2 cell-line. The freeze-dried bacteria exhibited a significantly different zeta potential compared to fresh cultures; however, this condition could be restored by rehydration with the lysine mixture. Recovery of the surface charge was found to influence adhesion ability to the Caco-2 cell-line. The optimum lysine concentration of the formulation, designated "Zeta-bio", was found to be 0.03 M for improving the viability of Lactiplantibacillus plantarum Lp-115 by up to 13.86% and a 7-strain mixture (400B) to 41.99% compared to the control rehydrated with distilled water. In addition, the lysine Zeta-bio formulation notably increased the adherence ability of lyophilized Lp-115 to the Caco-2 cell-line after subjected to the in vitro stress conditions of the simulated gastrointestinal tract passage.

• Geomechanics and Engineering
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• v.27 no.6
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• pp.551-560
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• 2021

Due to the low strength and high compressibility characteristics, the loess deposits tunnels are prone to large deformations and collapse. An accurate stability evaluation for loess deposits is of considerable significance in deformation control and safety work during tunnel construction. 37 groups of representative data based on real loess deposits cases were adopted to establish the stability evaluation model for the tunnel project in Yan'an, China. Physical and mechanical indices, including water content, cohesion, internal friction angle, elastic modulus, and poisson ratio are selected as index system on the stability level of loess. The data set is randomly divided into 80% as the training set and 20% as the test set. Firstly, principal component analysis (PCA) is used to convert the five index system to three linearly independent principal components X1, X2 and X3. Then, the principal components were used as input vectors for probabilistic neural network (PNN) to map the nonlinear relationship between the index system and stability level of loess. Furthermore, Leave-One-Out cross validation was applied for the training set to find the suitable smoothing factor. At last, the established model with the target smoothing factor 0.04 was applied for the test set, and a 100% prediction accuracy rate was obtained. This intelligent classification method for loess deposits can be easily conducted, which has wide potential applications in evaluating loess deposits.