• 대한기계학회논문집A
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• 제33권6호
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• pp.568-572
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• 2009

We present a ternary microfluidic multiplexer unit, capable to address three flow channels using a pair of control lines with two different threshold pressure valves. The previous binary multiplexer unit addresses only two flow channels using a pair of control line with identical threshold pressure valves, thus addressing $2^{n/2}$ flow channels using n control lines. The present ternary multiplexer addressing three flow channels using a pair of control lines, however, is capable to address $3^{n/2}$ flow channels using n control lines with two different threshold pressure valves. In the experimental study, we characterized the threshold pressure and the response time of the valves used in the ternary multiplexer. From the experimental observation, we also verified that the present ternary multiplexer unit could be operated by two equivalent valve operating conditions: the different static pressures and dynamic pressures at different duty ratio. And then, $3{\times}3$ well array stacking ternary multiplexers in serial is addressed in cross and plus patterns, thus demonstrating the individual flow channel addressing capability of the ternary multiplexer. Thus, the present ternary multiplexer reduces the number of control lines for addressing flow channels, achieving the high well control efficiency required for simple and compact microfluidic systems.

• 제어로봇시스템학회:학술대회논문집
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• 제어로봇시스템학회 1994년도 Proceedings of the Korea Automatic Control Conference, 9th (KACC) ; Taejeon, Korea; 17-20 Oct. 1994
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• pp.379-384
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• 1994

In this paper, a fish-drying control method is proposed, which utilizes prediction of proper change in- weight of material fish based on skilled worker's performance. The function of the proposed system is largely broken down into two procedures: The procedure before drying and the one during drying. The procedure before drying is the determination of necessary drying conditions and the required drying time. Required drying time and proper changes in weight for a specific product are obtained by using fuzzy inference and regression models. The procedure during drying is the prediction of the state of material at the end of drying, or the state of product and regulation of drying conditions to attain the prescribed goal before drying. The prediction of product is obtained by using a set of linear-differential equations obtained by the authors' previous work. Drying conditions are regulated by using fuzzy inference. A good agreement between the results of simulation and experiments is obtained, which implies the usefulness of the present control method.

• 한국정밀공학회:학술대회논문집
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• 한국정밀공학회 2003년도 춘계학술대회 논문집
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• pp.640-643
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• 2003

We present investigations of the surface micromachining for transparent glass substrate, e.g. soda lime glass using tightly focused 800nm Ti:sapphire femtosecond laser. In this study, experiment conditions such as laser intensity, scanning speed, focus position were controlled as variable parameters to decide optimal machining conditions. This study shows clearly that laser intensity and scanning speed are dominant factors for good surface morphology. Using the optimal conditions, grooves with 50${\mu}{\textrm}{m}$ line width were fabricated on glass substrate and their surface morphologies were investigated from SEM image.

• International Journal of Aeronautical and Space Sciences
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• 제16권4호
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• pp.529-536
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• 2015

Various R&D programs for rocket-based combined cycle (RBCC) engines have progressed worldwide for the space development and the defense applications. As a way toward indigenous domestic RBCC program, a preliminary design of flight test conditions was studied in this study for a sub-scale RBCC engine using a sounding rocket. Launch and flight profiles were calculated for several booster options and compared with that of HyShot II program. The result shows that the Korea Sounding Rocket-II (KSR-II) is a proper candidate to perform the flight test available in Korea. The recommend flight test conditions with KSR-II are Mach 6.0 with a test vehicle of 230 kg and Mach 7.4 with 50 kg. Present study will soon be followed by a design of sub-scale RBCC for a flight test using a sounding rocket.

• Structural Engineering and Mechanics
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• 제74권5호
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• pp.577-588
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• 2020

A unified solution is presented for the buckling analysis of rectangular laminated composite plates with elastically restrained edges. The plate is subjected to biaxial in-plane compression, and the boundary conditions are simulated by employing uniform distribution of linear and rotational springs at all edges. The critical values of buckling loads and corresponding modes are calculated based on classical lamination theory and using the Ritz method. The deflection function is defined based on simple polynomials without any auxiliary function. The verifications of the current study are carried out with available combinations of classic boundary conditions in the literature. Through parametric study with a wide range of spring factors with some classical as well as some not classical boundary conditions, competency of the present model of boundary conditions is proved.

• 한국응용과학기술학회지
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• 제28권3호
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• pp.284-289
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• 2011

All spectroscopic methods used in this work indicate the instability of tungstophosphates in aqueous solutions and considerable dependence on pH with regard to the dominant species present in the solution. UV spectroscopy indicates that some changes occur in the system but they cannot be specified. IR spectroscopy gives more information on the identification of the dominant species as a function the pH of the solution. NMR spectroscopy provides unique data, which can be used for more accurate interpretation of changes in the solution of various pH values. In the case of aqueous solutions of tungstophosphates, the parent anion was present only in a very acidic solution of ca. pH 1. Some differences in interpretation of the molecular species present under various experimental conditions can be ascribed to some extent to the diversity of chemical shifts of NMR. Under physiological conditions attained with the addition of NaOH, tungstophosphates was dominantly present in the form of the lacunary monovacant anion.

• 한국안전학회지
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• 제30권5호
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• pp.131-137
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• 2015

For the evaluation of safety margin of a nuclear power plant using a conservative methodology, the influence of applied assumptions such as initial conditions and boundary conditions needs to be assessed deliberately. Usually, a combination of the most conservative initial conditions is determined, and the safety margin for the transient is evaluated through the analysis for this conservative conditions. In existing conservative methodologies, a most-conservative condition is searched through the analyses for the maximum, minimum, and nominal values of the major parameters. In the present study, we investigates a new approach which can be applied to choose a most-conservative initial condition effectively when a best-estimate computer code and a conservative evaluation methodology are utilized for the evaluation of safety margin of transients. By constituting the band of various initial conditions using the random sampling of input parameters, the sensitivity study for various parameters are performed systematically. A method of sampling the value of control or operation parameters for a certain range is adopted by use of MOSAIQUE program, which enables to minimize the efforts for achieving the steady-state for various different conditions. A representative control parameter is identified, which governs the reactor coolant flow rate, pressurizer pressure, pressurizer level, and steam generator level, respectively. It is shown that an appropriate distribution of input parameter is obtained by adjusting the range and distribution of the control parameter.

• Smart Structures and Systems
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• 제16권5호
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• pp.853-872
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• 2015

Numerical analysis of large amplitude free vibration behaviour of laminated composite spherical shell panel embedded with the piezoelectric layer is presented in this article. For the investigation purpose, a general nonlinear mathematical model has been developed using higher order shear deformation mid-plane kinematics and Green-Lagrange nonlinearity. In addition, all the nonlinear higher order terms are included in the present mathematical model to achieve any general case. The nonlinear governing equation of freely vibrated shell panel is obtained using Hamilton's principle and discretised using isoparametric finite element steps. The desired nonlinear solutions are computed numerically through a direct iterative method. The validity of present nonlinear model has been checked by comparing the responses to those available published literature. In order to examine the efficacy and applicability of the present developed model, few numerical examples are solved for different geometrical parameters (fibre orientation, thickness ratio, aspect ratio, curvature ratio, support conditions and amplitude ratio) with and/or without piezo embedded layers and discussed in details.

• Asian-Australasian Journal of Animal Sciences
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• 제24권11호
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• pp.1529-1540
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• 2011

Silencing of a specific gene using RNAi (RNA interference) is a valuable tool for functional analysis of a target gene. However, information on RNAi for analysis of gene function in farm animals is relatively nil. In the present study, we have validated the interfering effects of siRNA (small interfering RNA) using both quantitative and qualitative gene silencing in buffalo granulosa cells. Qualitative gene knockdown was validated using a fluorescent vector, enhanced green fluorescence protein (EGFP) and fluorescently labeled siRNA (Cy3) duplex. While quantitatively, siRNA targeted against the luciferase and CYP19 mRNA was used to validate the technique. CYP19 gene, a candidate fertility gene, was selected as a model to demonstrate the technique optimization. However, to sustain the expression of CYP19 gene in culture conditions using serum is difficult because granulosa cells have the tendency to luteinize in presence of serum. Therefore, serum free culture conditions were optimized for transfection and were found to be more suitable for the maintenance of CYP19 gene transcripts in comparison to culture conditions with serum. Decline in fluorescence intensity of green fluorescent protein (EGFP) was observed following co-transfection with plasmid generating siRNA targeted against EGFP gene. Quantitative decrease in luminescence was seen when co-transfected with siRNA against the luciferase gene. A significant suppressive effect on the mRNA levels of CYP19 gene at 100 nM siRNA concentration was observed. Also, measurement of estradiol levels using ELISA (enzyme-linked immunosorbent assay) showed a significant decline in comparison to control. In conclusion, the present study validated gene silencing using RNAi in cultured buffalo granulosa cells which can be used as an effective tool for functional analysis of target genes.

• 한국주거학회논문집
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• 제17권5호
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• pp.55-65
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• 2006

The objective of this study is to provide new direction on house design in consideration of the PC users' life pattern. This study found that PC use present conditions at home, demand for PC using space, and requirements for PC using area were analyzed. A survey was taken by 1108 residences, and the SPSS win.10 was applied for analysis. The survey was conducted to see the computer usage at home, demand and satisfaction for the PC using space. The results of this study were as follows; forty-seven percent of respondents said they used the PC in son's and daughter's room while 36.8% answered living room. It reflects that the whole family want to use the PC in the same area, or for their children's convenience. Users' consciousness was analyzed four factor, which were 'physical inconvenience:'satisfactions in PC position', 'inconvenience caused by narrowness', 'notion of privacy'. PC users' consciousness had significant differences in the satisfaction by PC users' characteristic and housing characteristic. It was to be classified that the PC use space into several pattern according to furniture arrangement, and channel pattern had the highest satisfaction among those pattern. Through this study, it is expected that new housing designs need to be made to reflect lese requirements into interior design and house plan for PC user's needs.