• Advances in nano research
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• 제12권5호
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• pp.501-514
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• 2022

This study aimed to investigate the effects and potential mechanisms of Chikusetsusaponin V (CsV) on endothelial nitric oxide synthase (eNOS) and vascular endothelial cell functions. Different concentrations of CsV were added to animal models, bovine aorta endothelial cells (BAECs) and human umbilical vein endothelial cells (HUVECs) cultured in vitro. qPCR, Western blotting (WB), and B ultrasound were performed to explore the effects of CsV on mouse endothelial cell functions, vascular stiffness and cellular eNOS mRNA, protein expression and NO release. Bioinformatics analysis, network pharmacology, molecular docking and protein mass spectrometry analysis were conducted to jointly predict the upstream transcription factors of eNOS. Furthermore, pulldown and ChIP and dual luciferase assays were employed for subsequent verification. At the presence or absence of CsV stimulation, either overexpression or knockdown of purine rich element binding protein A (PURA) was conducted, and PCR assay was employed to detect PURA and eNOS mRNA expressions, Western blot was used to detect PURA and eNOS protein expressions, cell NO release and serum NO levels. Tube formation experiment was conducted to detect the tube forming capability of HUVECs cells. The animal vasodilation function test detected the vasodilation functions. Ultrasonic detection was performed to determine the mouse aortic arch pulse wave velocity to identify aortic stiffness. CsV stimulus on bovine aortic cells revealed that CsV could upregulate eNOS protein levels in vascular endothelial cells in a concentration and time dependent manner. The expression levels of eNOS mRNA and phosphorylation sites Ser1177, Ser633 and Thr495 increased significantly after CsV stimulation. Meanwhile, CsV could also enhance the tube forming capability of HUVECs cells. Following the mice were gavaged using CsV, the eNOS protein level of mouse aortic endothelial cells was upregulated in a concentration- and time-dependent manner, and serum NO release and vasodilation ability were simultaneously elevated whereas arterial stiffness was alleviated. The pulldown, ChIP and dual luciferase assays demonstrated that PURA could bind to the eNOS promoter and facilitate the transcription of eNOS. Under the conditions of presence or absence of CsV stimulation, overexpression or knockdown of PURA indicated that the effect of CsV on vascular endothelial function and eNOS was weakened following PURA gene silence, whereas overexpression of PURA gene could enhance the effect of CsV upregulating eNOS expression. CsV could promote NO release from endothelial cells by upregulating the expression of PURA/eNOS pathway, improve endothelial cell functions, enhance vasodilation capability, and alleviate vessel stiffness. The present study plays a role in offering a theoretical basis for the development and application of CsV in vascular function improvement, and it also provides a more comprehensive understanding of the pharmacodynamics of CsV.

• 생명과학회지
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• 제32권6호
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• pp.482-490
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• 2022

근육 양과 근력의 감소에 의한 근감소증은 다양한 병적 상태의 악화, 삶의 질 저하, 사망률의 증가를 동반하며, 기대수명이 증가함에 따라 앞으로도 유병률은 계속 증가할 것이다. 노화 과정에서 만성 산화 스트레스와 염증 반응의 증가는 골격근 소실의 주요 원인으로 작용하며, 에너지 대사에 필수적인 미토콘드리아의 기능 장애와 관련된 자가포식 및 세포사멸 신호의 교란은 근육 단백질의 손실을 가속화한다. 오래전부터 각종 질병의 예방 및 치료에 널리 사용되어온 동충하초의 주요 생리활성물질인 cordycepin의 약리학적 효과는 항산화 및 항염증 작용과 직접적인 관련이 있는 것으로 알려져 있다. 본 총설에서는 근감소증의 예방과 치료에의 적용을 위한 cordycepin의 세포사멸, 자가포식, 단백질의 이화작용 및 근육 재생에 중요한 위성세포의 활성에 대한 연관성을 제시하였다. 비록 현재까지 근감소증에 대한 cordycepin의 연구는 미진한 수준이지만, 그동안의 연구 결과에서 cordycepin은 노화로 인한 미토콘드리아 기능 약화를 억제하고 근육 단백질의 분해를 차단하는 데 기여할 수 있음을 알 수 있다. 또한 근세포 손상에 대한 cordycepin의 보호 효과는 항산화 및 항염증 활성과 밀접한 관련이 있음을 제안한다. 따라서 근세포의 노화방지에 관여하는 cordycepin의 분자생물학적 기전을 중심으로 보다 지속적인 기초 연구가 필요할 것으로 사료된다.

• 대한기관윤리심의기구협의회지
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• 제4권1호
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• pp.16-22
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• 2022

Purpose: The traditional ethical study only suggests a blurred insight on the research using medical big data, especially in this rapid-changing and demanding environment which is called "4th Industry Revolution." Current institutional/ethical issues in big data research need to approach with the thoughtful insight of past ethical study reflecting the understanding of present conditions of this study. This study aims to examine the ethical issues that are emerging in recent health care big data research. So, this study aims to survey the public perceptions on of health care big data as part of the process of public discourse and the acceptance of the utility and provision of big data research as a subject of health care information. In addition, the emerging ethical challenges and how to comply with ethical principles in accordance with principles of the Belmont report will be discussed. Methods: Survey was conducted from June 3th August to 6th September 2020. The online survey was conducted through voluntary participation through Internet users. A total of 319 people who completed the survey (±5.49%P [95% confidence level] were analyzed. Results: In the area of the public's perspective, the survey showed that the medical information is useful for new medical development, but it is also necessary to obtain consents from subjects in order to use that medical information for various research purposes. In addition, many people were more concerned about the possibility of re-identifying personal information in medical big data. Therefore, they mentioned the necessity of transparency and privacy protection in the use of medical information. Conclusion: Big data on medical care is a core resource for the development of medicine directly related to human life, and it is necessary to open up medical data in order to realize the public good. But the ethical principles should not be overlooked. The right to self-determination must be guaranteed by means of clear, diverse consent or withdrawal of subjects, and processed in a lawful, fair and transparent manner in the processing of personal information. In addition, scientific and ethical validity of medical big data research is indispensable. Such ethical healthcare data is the only key that will lead to innovation in the future.

• 한국근적외분광분석학회:학술대회논문집
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• 한국근적외분광분석학회 2001년도 NIR-2001
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• pp.1246-1246
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• 2001

The quality of meat is highly variable in many properties. This variability originates from both animal production and meat processing. At the pre-slaughter stage, animal factors such as breed, sex, age contribute to this variability. Environmental factors include feeding, rearing, transport and conditions just before slaughter (Hildrum et al., 1995). Meat can be presented in a variety of forms, each offering different opportunities for adulteration and contamination. This has imposed great pressure on the food manufacturing industry to guarantee the safety of meat. Tissue and muscle speciation of flesh foods, as well as speciation of animal derived by-products fed to all classes of domestic animals, are now perhaps the most important uncertainty which the food industry must resolve to allay consumer concern. Recently, there is a demand for rapid and low cost methods of direct quality measurements in both food and food ingredients (including high performance liquid chromatography (HPLC), thin layer chromatography (TLC), enzymatic and inmunological tests (e.g. ELISA test) and physical tests) to establish their authenticity and hence guarantee the quality of products manufactured for consumers (Holland et al., 1998). The use of Near Infrared Reflectance Spectroscopy (NIRS) for the rapid, precise and non-destructive analysis of a wide range of organic materials has been comprehensively documented (Osborne et at., 1993). Most of the established methods have involved the development of NIRS calibrations for the quantitative prediction of composition in meat (Ben-Gera and Norris, 1968; Lanza, 1983; Clark and Short, 1994). This was a rational strategy to pursue during the initial stages of its application, given the type of equipment available, the state of development of the emerging discipline of chemometrics and the overwhelming commercial interest in solving such problems (Downey, 1994). One of the advantages of NIRS technology is not only to assess chemical structures through the analysis of the molecular bonds in the near infrared spectrum, but also to build an optical model characteristic of the sample which behaves like the “finger print” of the sample. This opens the possibility of using spectra to determine complex attributes of organic structures, which are related to molecular chromophores, organoleptic scores and sensory characteristics (Hildrum et al., 1994, 1995; Park et al., 1998). In addition, the application of statistical packages like principal component or discriminant analysis provides the possibility to understand the optical properties of the sample and make a classification without the chemical information. The objectives of this present work were: (1) to examine two methods of sample presentation to the instrument (intact and minced) and (2) to explore the use of principal component analysis (PCA) and Soft Independent Modelling of class Analogy (SIMCA) to classify muscles by quality attributes. Seventy-eight (n: 78) beef muscles (m. longissimus dorsi) from Hereford breed of cattle were used. The samples were scanned in a NIRS monochromator instrument (NIR Systems 6500, Silver Spring, MD, USA) in reflectance mode (log 1/R). Both intact and minced presentation to the instrument were explored. Qualitative analysis of optical information through PCA and SIMCA analysis showed differences in muscles resulting from two different feeding systems.

• 한국지반공학회논문집
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• 제24권11호
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• pp.121-131
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• 2008

슬러리월 연직차수벽 시공시, 지반을 트렌치 형태로 굴착하고, 굴착시공 중 굴착면 붕괴에 대한 안정성을 유지하기 위하여 벤토나이트와 물을 혼합한 슬러리를 트렌치에 채운다. 이렇게 채워진 벤토나이트 슬러리로 인하여 필터케이크(filter cake)이라 불리는 얇고 투수성이 낮은 층이 트렌치벽 표면에 형성되며, 슬러리가 제거 된 이후에도 굴착면에 잔존하여 연직차수벽에서의 순간변위시험(slug test) 해석 결과에 중요한 영향을 미친다. 본 연구에서는 기존에 개발된 수치해석 프로그램 Slug-3D를 수정하여 순간변위시험 해석시 필터케이크의 영향을 고려하는 방법을 제시하고 이를 통하여 정확한 연직차수벽의 투수계수를 산출하도록 하였다. 필터케이크의 영향을 고려하기 위하여 Slug-3D에서는 차수벽과 토양층의 경계면에 불투수조건(no-flux)을 경계조건으로 설정하여 해석을 수행하였다 또한 투수계수 산정에서 기존의 타입커브법(type curve method)을 이용하기 위하여 개선된 type curve를 도출하였고, 선형 커브피팅법(line-fitting method)를 필터케이크가 존재하는 연직차수벽에 적용할 수 있도록 수정 보완하였다. 본 논문에서 제안된 필터케이크를 고려한 해석방법은 EMCON(1995)에 의해 수행된 현장시험 결과의 재해석을 통하여 타당성을 검토하였다. 또한, 본 연구에서 얻어진 결과를 Choi and Daniel(2006)에 의해 수행된 필터케이크가 존재하지 않는 경우의 순간변위시험 해석결과와 비교를 통해 필터케이크 고려가 반드시 필요함을 보여준다.

• 한국컴퓨터정보학회논문지
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• 제14권10호
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• pp.241-249
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• 2009

동영상 검색이나 축약과 같은 동영상 분석을 위해 동영상 어노테이션 기술이나 동영상 정보 표현에 대한 다양한 연구가 있어왔다. 이를 위해 본 논문은 대화 참여자 결점을 위한 영상적 요소와 이러한 요소를 이용하여 Character-net 표현을 개선하는 방법을 제안한다. 기존 Character-net이 자막이 뜨는 시간에 나타나는 등장인물들만을 대화참여자로 고려하므로 일부의 청자를 제외시키는 문제점이 있다. 대화 참여자는 대화상황 파악의 극히 중요한 요소로 동영상 검색 시에 기준이 될 수 있으며 동영상의 이야기 전개를 이끌어 나간다. 대화 참여자를 결정하기 위한 영상적 요소에는 자막의 유무, 장면, 인물 등장순서, 시선방향, 패턴, 입의 움직임 등이 있다. 본 논문에서는 이러한 영상적 요소에 근거하여 대화 참여자를 판단하고 동영상 표현방법인 Character-net을 개선하고자 한다. 제안한 여러 요소들이 결합되고 일정한 조건이 만족되었을 때 대화참여자를 정확히 검출할 수 있다. 따라서 본 논문에서는 대화참여자를 결정하기 위한 영상적 요소들을 제안하고 이를 통해 Character-net의 표현성능을 개선하고 실험을 통하여 제안된 방법론이 대화 참여자 판단의 정확성과 Character-net의 표현성능을 제고함을 증명하였다.

• 천문학회보
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• 제46권2호
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• pp.38.2-39
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• 2021

I will presents the analysis of the gas properties of the protoplanetary disk surrounding the young low-mass (about 1.2M_sun) triple star, GG Tau A. This work makes use of ALMA observations of rotational lines of CO (¹²CO, ¹³CO and C¹⁸O) together NOEMA observations of a few dozens of other molecules. While the CO emission gives information on the molecular layer close to the disk atmosphere, its less abundant isotopologues ¹³CO and C¹⁸O bring information much deeper in the molecular layer. I will present the analysis of the morphology and kinematics of the gas disk using the CO isotopologues. A radiative transfer model of the ring in CO isotopologues will also be presented. The subtraction of this model from the original data reveals the weak emission of the molecular gas lying inside the cavity. Thus, I am able to evaluate the properties of the gas inside the cavity, such as the gas dynamics, excitation conditions, and the amount of mass in the cavity. High angular resolution observations of CO reveals sprials induced by embedded planet(s) located near the 3:2:1 mean-motion resonance that help to explain the special morphology of the circumbinary disk. I also discuss some chemical properties of the GG Tau A disk. I report the first detection of H2S and C₂S in a protoplanetary disk. The molecule abundance relative to ¹³CO of about twenties other molecules will also be given. In GG Tau A, the detections of rare molecules such as H₂S and C₂S have been probably possible because the disk is more massive (a factor about 3-5) than other disks where the molecules was searched. Such a large disk mass makes the system suitable to detect rare molecules and to study cold-chemistry in protoplanetary disks.

• 대한토목학회논문집
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• 제28권5D호
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• pp.617-625
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• 2008

중소도시의 경우 저밀도 지역의 만연과 산재한 통행 기종점으로 인해 양질의 대중교통서비스 제공에 어려움을 겪고 있다. 동시환승체계는 이 같은 지역에 적절한 대중교통서비스 제공이 가능하도록 운행계획이 수립된 노선망으로, 도입 시 서비스 제고와 운행효율성 증대 등 다양한 효과가 발생할 수 있다. 본 연구는 동시환승체계의 현실적 적용을 위한 접근방법론 제시와 도입 시 발생 가능한 기대효과의 파악과 분석을 목적으로, 동시환승체계의 이론적 근거를 살펴보고 구축의 방법론적 접근법을 제시하였고, 전형적인 저밀도 지역인 양산시의 기존 시내버스 노선에 적용하여 그 효과를 분석하였다. 이를 위해 양산시의 양산시외버스터미널을 통과하는 11개 시내버스 노선을 양산터미널을 환승지점으로 동시환승체계를 구축하였다. 그 결과 노선 간 환승시간이 크게 감소하였고, 기존 차량대수를 유지함에도 불구하고 높은 빈도의 서비스 제공이 가능해지는 등 서비스 질이 개선 되었다. 또한 기존 노선망의 서비스 권역을 그대로 유지함에도 불구하고 노선단축 등으로 인한 총 운행거리와 요구차량대수가 감소하는 효과도 나타났다. 본 연구의 결과는 유사한 문제에 처한 여타 도시에 적용이 가능하며, 계획가와 정책가에게는 노선 구축을 위한 지침과 정책결정의 근거를 제공할 것이다.

• Structural Engineering and Mechanics
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• 제87권3호
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• pp.283-296
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• 2023

Free-vibration and buckling analyses of plate problems are investigated with the aid of the strain gradient notation finite element method (SGN-FEM). As SGN-FEM employs physically interpretable polynomials in developing finite elements, parasitic shear sources, which are the cause of shear locking, can be precisely identified and subsequently eliminated. This allows two mutually complementary objectives to be defined in this work, namely, evaluate the efficiency of free-vibration and buckling results provided by corrected models, and study the severity of parasitic shear effects on plate models performance. Parasitic shear are flexural terms erroneously present in shear strain polynomials. It is reviewed here that six parasitic shear terms arise during the formulation of the four-node Mindlin plate element. Two parasitic shear terms have been identified in the in-plane shear strain polynomial while other two have been identified in each of the transverse shear strain polynomials. The element is corrected a-priori, i.e., during development, by simply removing the spurious terms from the shear strain polynomials. The computational implementation of the element in its two versions, namely, containing the parasitic shear terms (PS) and corrected for parasitic shear (SG), allows for assessments of the accuracy of results and of the deleterious effects of parasitic shear in free vibration and buckling analyses. This assessment of the parasitic shear effects is a novelty of this work. Validation of the SG model is done comparing its results with analytical results and results provided by other numerical procedures. Analyses are performed for square plates with different thickness-to-length ratios and boundary conditions. Results for thin plates provided by the PS model do not converge to the correct solutions, which indicates that parasitic shear must be eliminated. That is, analysts should not rely on refinement alone. For thick plates, PS model results can be considered acceptable as deleterious effects are really critical in thin plates. On the other hand, results provided by the SG model converge well for both thin and thick plates. The effectiveness of the SG model is established via high-accuracy results obtained in several examples. It is concluded that corrected SGN-FEM models are efficient alternatives for free-vibration and buckling analysis of Mindlin plate problems, and that precise elimination of parasitic shear is a requirement for sound analyses.

• Asia Marketing Journal
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• 제15권1호
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• pp.83-105
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• 2013

During recent decades, the number of mixed attribute products (henceforth mixed products), which have both utilitarian and hedonic benefits, has increased dramatically. Despite these products' growing popularity, academic research has paid little attention to them, and there remains a gap between theory and the real world. Hence, our study was undertaken to understand consumers' perceptions about and behaviors toward mixed products, as well as factors affecting the evaluation and choice of these products. We divided mixed attribute products into two categories: mixed utilitarian products (utilitarian products adding hedonic attributes) and mixed hedonic products (hedonic products adding utilitarian attributes). We then showed how adding different attributes affects consumers' perception, willingness to pay (WTP), and the choice of mixed attribute products compared to pure utilitarian or pure hedonic products. We conducted an experiment using a within-subject design. A total of 160 office workers and college students participated in the study. The pure utilitarian product used in the study was orange juice, and the mixed utilitarian product was carbonated orange juice. The pure hedonic product was chocolate, and the mixed hedonic product was polyphenol enriched chocolate. Results showed that consumers perceived a mixed utilitarian product to be less utilitarian, less pleasurable and more guilty than a pure utilitarian product. On the other hand, a mixed hedonic product was perceived to be more utilitarian, less pleasurable and less guilty than a pure hedonic product. Also, WTP for a mixed hedonic product was higher than WTP for a pure hedonic product, but WTP was lower for a mixed utilitarian product than for a pure utilitarian product. Furthermore, mixed hedonic products were likely to be evaluated more favorably when they were presented together with pure hedonic products, more so than when they were presented alone. Finally, when compared to low self-control participants, high self-control participants chose mixed hedonic products more frequently. The present study contributes to the existing literature on utilitarian and hedonic consumption by adding to the sparse literature on the consumption of products that have both utilitarian and hedonic purposes. Also, our research findings provide several useful implications for practitioners in related fields. First, the current study provides marketers with a useful guide for understanding consumers' perceptions of these types of products, and helps to predict how adding different attributes influences these products. Second, this study has examined the conditions that may moderate the evaluation and choice of hedonic base products and this finding will serve as a good reference for marketers of mixed hedonic products in marketing communication strategy, in-store marketing and targeting. Specifically, comparative advertising with a pure hedonic product will be beneficial for a mixed hedonic product. Also, displaying mixed hedonic products near pure hedonic products may enhance the effectiveness of in-store marketing of mixed hedonic products.