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Analysis of Trans-splicing Transcripts in Embryonic Stem Cell (배아줄기세포에서 트랜스 스플라이싱 전사체의 분석)

  • Ha, Hong-Seok;Huh, Jae-Won;Kim, Dae-Soo;Park, Sang-Je;Bae, Jin-Han;Ahn, Kung;Yun, Se-Eun;Kim, Heui-Soo
    • Journal of Life Science
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    • v.19 no.4
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    • pp.549-552
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    • 2009
  • Genetic mutations by gene fusion result from chromosomal rearrangement, trans-splicing, and intergenic splicing. Trans-splicing is a phenomenon in which two pre-mRNAs grow together into one. We analyzed the trans-splicing products in embryonic stem cells. By using bioinformatic tools, 70 trans-splicing transcripts were identified. They are classified into 6 types according to fusion pattern: 5'UTR-5'UTR, 5'UTR-3'UTR, 3'UTR-3'UTR, 5'UTR-CDS, 3'UTR-CDS, CDS-CDS. The fusion products are more abundant in CDS regions than in UTR regions, which contain multiple intron numbers. Chromosome analysis showing gene fusion via trans-splicing indicated that chromosomes 17 and 19 were activated. These data are of great use for further studies in relation to fusion genes and human diseases.

The cooperation of civil aviation and legal and political issues related to direct route operation between South and North Korea (남북간 민간항공협력과 직항로 개설 운영상의 법적 정책적 과제)

  • Kim, Maeng-Sern;Hong, Soon-KiI
    • The Korean Journal of Air & Space Law and Policy
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    • v.17
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    • pp.111-132
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    • 2003
  • The air transport industry is the most important as means of human exchange between the countries. Because the spread effect and the durability by aviation cooperation between the countries are much higher than any other industry, a research about air transport industry is very important to allied industry field as well as national policy about International cooperation and integration. Specially, according to the economic interchange with North Korea becomes active, the role of air transport as related traffic network with North Korea becomes more important. The number of flights is increasing sharply after South-North summit meeting, and two sides established and are using temporary direct route between South-North Korea. When we consider that the number of flights utilizing temporary direct route is increasing every year, It is not desirable to use temporary routes continuously because the current agreement between South and North cant be reliable far the case of unexpected circumstance. In addition, the current agreement is not based on the international standards. The paper is to study the condition to promote the coordination of civil aviation in the whole Korean peninsula. As known, the aviation system in North Korea is mainly operated by military unit. The study will review the current status of air transport system of South and North and the effective way of cooperation of civil aviation between both sides. The cooperation between governments as well as between airlines is studied. The establishment of Air Traffic Service Agreement is going to be handled heavily because the stable air traffic service is the most required base for the operation of air transport. The authors also try to find a way to support the development of infrastructure of aviation industry in North Korea.

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The Evaluation of RPM Change in X-Smart According to Power Source: Endodontic Wireless Electronic Motor (근관치료용 무선전기모터인 X-Smart의 전원의 종류에 따른 RPM 변화의 평가)

  • Park, Se-Hee;Jeong, Dong-Bin;Cho, Kyung-Mo;Kim, Jin-Woo
    • Journal of Dental Rehabilitation and Applied Science
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    • v.27 no.1
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    • pp.91-97
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    • 2011
  • The purpose of this study was to evaluate RPM maintenance in X-Smart according to the power supplied by the AC adapter and rechargeable battery. Five new X-Smart were used in this study. A handpiece with rubber disk which has reflective tape was mounted on the vice. To measure the RPM, a non-contact type digital tachometer was used. RPM measurements were recorded every 5 minutes to 180 minutes and repeated 3 times per motor from each power source. To evaluate a difference of the RPM changes, two-way repeated measures were performed using $SPSS^{TM}$ 14.0. All tests were conducted at the 95 percent confidence level. There was no significant difference in the RPM change between the power sources. Continuous use of X-Smart with a rechargeable battery for up to 180 minutes could be safely used as an endodontic wireless motor with a reliable RPM maintenance.

Empirical Evaluation on the Size of E-Book Devices in User Comprehensive View (사용자의 이해력 관점에서 전자책 장치의 크기에 관한 실험적 평가)

  • Son, Yong-Bum;Kim, Young-Hak
    • The Journal of the Korea Contents Association
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    • v.12 no.8
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    • pp.167-177
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    • 2012
  • Recently, with the rapid development of information technology the field of e-book market is growing rapidly. The choice of an e-book device to improve user's comprehension is one of very important elements. The effectiveness evaluation between e-books and paper books has been studied previously, but there have not been progressed actively researches on the size of e-book devices based on user's comprehension. Considering these aspects, we in this paper selected e-book devices such as currently available PDA, netbook, and notebook, and then carried out the experiment about which device has the highest user's comprehension depending on the size of e-book devices. Understanding and memory about the content on the display were set as main factors in order to evaluate user's comprehension. We prepared in advance multiple examples of e-books and English words with similar difficulty, and evaluated user's comprehension through answering questions for each example after doing the experiment. 90 undergraduate students who use most widely e-books participated in the experiment, and the result was analyzed using SPSS statistical package. The experiment result showed that user's comprehension was higher in e-book device with middle size rather than the one with big size in display size.

Genomic Polymorphism Analysis using Microsatellite Markers in Gyeongju Donggyeong Dogs

  • Kim, Seung-Chang;Kim, Lee-Kyung;Choi, Seog-Kyu;Park, Chang-Min;Park, Sun-Ae;Cho, Yong-Min;Lim, Dajeong;Chai, Han-Ha;Lee, Seung-Hwan;Lee, Ji-Woong;Sun, Sang-Soo;Choi, Bong-Hwan
    • Reproductive and Developmental Biology
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    • v.36 no.4
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    • pp.243-248
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    • 2012
  • This study was conducted to find a useful marker for gene polymorphism analysis using Microsatellite marker (MS marker) in Gyeongju Donggyeong dog. Twenty three MS marker analyzed the genetic features of DNA using 100 Gyeongju Donggyeong dogs in Gyeongju area. It was performed multiplex PCR with 3 set primer divided 9, 10 and 4 by analysis of conditions among MS markers. The results were calculated heterozygosity, polymorphic information content (PIC), allele frequency and number of allele at each locus using Microsatellite Toolkit software and Cervus 3.0 program. Total 148 alleles were genotyped to determine and average 6.43 alleles was detected. FH3381 had the highest of 15 alleles and FH2834 had the lowest of 2 alleles. Expected heterozygosity had a wide range from 0.282 to 0.876 and had average value of 0.6496. Also, Observed heterozygosity had a more wide range from 0.200 to 0.950 and had average value of 0.6404. PIC had range from 0.262 to 0.859 and average PIC was calculated 0.606. Especially, FH2998 represented the highest rate of observed heterozygosity of 0.950 and FH3381 represented the highest rate of expected heterozygosity of 0.876 and PIC of 0.859. The use of these markers was considered to be useful to study genetic traits of Gyeongju Donggyeong dog.

Future Prediction of Heat and Discomfort Indices based on two RCP Scenarios (기후변화 대응을 위한 RCP 시나리오 기반 국내 열지수와 불쾌지수 예측)

  • Lee, Suji;Kwon, Bo Yeon;Jung, Deaho;Jo, Kyunghee;Kim, Munseok;Ha, Seungmok;Kim, Heona;Kim, Byul Nim;Masud, M.A.;Lee, Eunil;Kim, Yongkuk
    • Atmosphere
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    • v.23 no.2
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    • pp.221-229
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    • 2013
  • There has been an increasing need to assess the effects of climate change on human health. It is hard to use climate data to evaluate health effects because such data have a grid format, which could not represent specific cities or provinces. Therefore, the grid-format climate data of South Korea based on RCP (Representative Concentration Pathway) scenarios were modified into area-format climate data according to the major cities or provinces of the country, up to the year 2100. Moreover, heat index (HI) and discomfort index (DI) databases were developed from the modified climate database. These databases will soon be available for experts via a Website, and the expected HI and DI of any place in the country, or at any time, can be found in the country's climate homepage (http://www.climate.go.kr). The HI and DI were analyzed by plotting the average indices every ten years, and by comparing cities or provinces with index level changes, using the geographic information system (GIS). Both the HI and DI are expected to continually increase from 2011 to 2100, and to reach the most dangerous level especially in August 2100. Among the major cities of South Korea, Gwangju showed the highest HI and DI, and Gangwon province is expected to be the least affected area in terms of HI and DI among all the country's provinces.

Estimation of Flows and Pollutant Loads from GIS Analysis using Cell-based Geospatial and Georgraphic Information Data (격자기반의 지형 및 지리정보자료와 GIS분석기법을 이용한 유역의 유출량 및 오염부하량 추정)

  • Cho, Jae-Myoung;Lee, Mi-Ran;Yun, Hong-Sik
    • Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
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    • v.29 no.4
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    • pp.381-392
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    • 2011
  • Pollutant loads calculated with unit factor method can not identity seasonal variations of pollutant inputs. Estimation of pollutant loads considering rainfall runoff can overcome these limits. SCS curve number method was applied to estimate runoff of each event of Koeup watershed of Koheung estuary lake. SCS curve numbers were calculated based upon land use, soil types of the catchment using GIS. Point and nonpoint source pollutant loads were summed up for total loads estimation. Those from nonpoint source were estimated by multiplying the calculated runoff and expected mean concentrations (EMC) presented by the Minister of Environment of Korea. DEM can present three dimensional views of a terrain, identity stream networks and flow accumulation. Furthermore, it can examine accumulated pollutant loads of specific point of a catchment. Therefore, cell based pollutant load estimation was attempted using DEM. ArcView was utilized to collect, store and manipulate spatial and attribute data of pollutant sources and features of the catchment. Cell-based DEM which was established by the GRID module of ARC/INFO was employed to estimate flows and pollutant loads.

The Preliminary Operator Risk Assessment of High Toxicological Pesticides in Korea (고독성 농약의 국내 농작업자 위해성 평가)

  • Hong, Soon-Sung;Jeong, Mi-Hye;Park, Kyung-Hun;You, Are-Sun;Park, Yeon-Ki;Lee, Je-Bong;Kim, Chan-Sub;Shin, Jin-Sup;Park, Jae-Eup
    • The Korean Journal of Pesticide Science
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    • v.14 no.2
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    • pp.116-122
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    • 2010
  • This study was carried out to estimate the risk of pesticide operators who use high toxicological pesticides. The class II (highly hazardous) pesticides registered in korea were 15 products, and 11 products were spray type pesticides at orchard file in 2009. The using information based on the pesticide label and the data searched through survey of actual condition on pesticides were used for calculate the pesticide operator exposure dose. The risk quotients of these pesticides against the pesticides operator were calculated as devide pesticide exposure dose by reference dose which were presented by EFSA (European Food Safety Authority), JMPR (Joint FAO Meeting on Pesticide Residues), and US/EPA (United States Environmental Protection Agency). Omethoate showed the highest risk quotient and the values were 338 and 75 when the operator spray using speed sprayer and motor sprayer respectively. Risk quotients of all class II pesticide were above 1. This result means that the risk potential of these pesticides are very high.

Opticla Angle Sensor Using Pseudorandom-code And Geometry-code (슈도 랜덤 코드와 기하학 코드를 이용한 광학적 Angle Sensor)

  • 김희성;도규봉
    • Journal of the Institute of Electronics Engineers of Korea SD
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    • v.41 no.2
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    • pp.27-32
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    • 2004
  • Absolute optical angle sensor is described that is an essentially digital opto-electronic device. Its purpose is to resolve the relative and absolute angle position of coded disk using Pseudorandom-code and Geometry-code. In this technique, the angular position of disk is determined in coarse sense first by Pseudorandom-code. A further fine angular position data based on Pixel count is obtained by Geometry-code which result 0.006$^{\circ}$ resolution of the system provided that 7 ${\mu}{\textrm}{m}$ line image sensor are used. The proposed technique is novel in a number of aspects, such that it has the non-contact reflective nature, high resolution of the system, relatively simple code pattern, and inherent digital nature of the sensor. And what is more the system can be easily modified to torque sensor by applying two coded disks in a manner that observe the difference in absolute angular displacement. The digital opto-electronic nature of the proposed sensor, along with its reporting of both torque and angle, makes the system ideal for use in intelligent vehicle systems. In this communication, we propose a technique that utilizes Pseudorandom-code and Geometry-code to determine accurate angular position of coded disk. We present the experimental results to demonstrate the validity of the idea.

License-Plate Extraction from Parking Regulation Images using Intensity Vector and Composite Color (복합 색상과 명암 벡터를 이용한 주차 단속 영상에서의 번호판 추출)

  • 권숙연;전병환
    • Journal of the Institute of Electronics Engineers of Korea CI
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    • v.40 no.6
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    • pp.47-55
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    • 2003
  • In this paper, we propose a new approach to detect peculiar features of license plates using intensity vector and composite color component in order to extract license plates from parking regulation images, which is captured in various locations around the front or the rear of cars at various times and places, and in which complex background is included. We fundamentally use both features that intensity value repeats frequently increasing and decreasing because intensity is obviously different at numerics and background, and that color is uniform in the area of license plates. First, we search each row at regular intervals starting from the bottom of a license-plate image, and we set up a rough region for a certain zone in which tile sign of intensity vector changes frequently enough and color of license plate is detected enough, assuming it as a candidate location of a license plate. And then, we extract an elaborate area of a license plate by projecting vertical edges horizontally and vertically. Here, type of cars, such as the urinate and the public, is easily classified according to the color of extracted plates. We used 200 actual regulation images, which are captured at various times and places, to evaluate the performance of the proposed method. As a result, the proposed method showed extraction rate of 96%, which is 9% higher than the previous method using only intensity vector.