• Title/Summary/Keyword: university laboratories

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Influence of Electrolyte Aging on Electrotinning in Phenolsulfonic Acid Bath (전기주석도금 반응에 미치는 PSA계 도금용액 노화의 영향)

  • Bae Dae Chul;Kim Tae Yeob;Cho Joon Hyung;Lee Jae Ryung;Chang Sam Kyu;Cho Kyung-mox
    • Journal of the Korean Electrochemical Society
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    • v.3 no.3
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    • pp.162-168
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    • 2000
  • The influence of phenolsulfunate concentrations on electroplating characteristics and electrochemical behaviors was investigated with a viewpoint of electrolyte aging using the circulation cell and potentiostate And comparison of tinplate coating appearance such as glossiness and Image clarify has been also studied with varying of phenolsulfonic acid (PSA) solutions. As the aging of electrolyte proceeded, the limiting current density was moved to a lower current density region by the limitation of mass transfer, and higher phenolsulfunate concentrations resulted in the narrower optimum current density range and deterioration of coating surface of tinplates. The difference of the limiting current density was not remarkable with increasing electrolyte temperature. Thus the electrolyte aging was attributed to the limitation of thermally-activated process such as mass transfer of reducible ions. It has also been considered that the accumulation of phenolsulfonate suppressed normal electrotinning reaction by reducing the mobility of stannous ions, taking into account of the smaller effect of electrolyte aging. Experiments showed similar polarization behavior between the electrolyte of high phenolsufonate solution and the aged one, which comes to conclude that the accumulation of phenolsulfonate is one of the major causes of electrolyte aging.

In vivo Genotoxicity of Silver Nanoparticles after 90-day Silver Nanoparticle Inhalation Exposure

  • Kim, Jin-Sik;Sung, Jae-Hyuck;Ji, Jun-Ho;Song, Kyung-Seuk;Lee, Ji-Hyun;Kang, Chang-Soo;Yu, Il-Je
    • Safety and Health at Work
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    • v.2 no.1
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    • pp.34-38
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    • 2011
  • Objectives: The antimicrobial activity of silver nanoparticles has resulted in their widespread use in many consumer products. Yet, despite their many advantages, it is also important to determine whether silver nanoparticles may represent a hazard to the environment and human health. Methods: Thus, to evaluate the genotoxic potential of silver nanoparticles, in vivo genotoxicity testing (OECD 474, in vivo micronuclei test) was conducted after exposing male and female Sprague-Dawley rats to silver nanoparticles by inhalation for 90 days according to OECD test guideline 413 (Subchronic Inhalation Toxicity: 90 Day Study) with a good laboratory practice system. The rats were exposed to silver nanoparticles (18 nm diameter) at concentrations of $0.7\;{\times}\;10^6$ particles/$cm^3$ (low dose), $1.4\;{\times}\;10^6$ particles/$cm^3$ (middle dose), and $2.9\;{\times}\;10^6$ particles/$cm^3$ (high dose) for 6 hr/day in an inhalation chamber for 90 days. The rats were killed 24 hr after the last administration, then the femurs were removed and the bone marrow collected and evaluated for micronucleus induction. Results: There were no statistically significant differences in the micronucleated polychromatic erythrocytes or in the ratio of polychromatic erythrocytes among the total erythrocytes after silver nanoparticle exposure when compared with the control. Conclusion: The present results suggest that exposure to silver nanoparticles by inhalation for 90 days does not induce genetic toxicity in male and female rat bone marrow in vivo.

Cloning and Expression of NS5 Region of Korean Type Hepatitis C Virus (한국형 C형 간염 바이러스의 NS5 지역 cDNA 클로닝과 발현)

  • Han, Dong-Pyou;Lee, Taek-Youl;Kim, Won-Bae;Kim, Byong-Moon;Chang, Mi-Yoon;Yang, Jai-Myung
    • The Journal of Korean Society of Virology
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    • v.27 no.2
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    • pp.115-128
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    • 1997
  • Three cDNA fragments located within NS5 region of HCV were synthesized by RT using viral RNA extracted from blood sample of Korean patient as a template. The cDNAs were amplified by PCR, cloned into the T-vector, and the nucleotide sequences were determined. Comparative analysis of the nucleotide and amino acid sequence of NS5 cDNAs showed that it is closely related with HCV type 1b. The cloned NS5 cDNA showed 91-94% homology at the nucleotide sequence level and 96-98% homology at the amino acid sequence level with several strains of the HCV type 1b. The NS5 cDNAs were subcloned into E. coli expression vectors to construct pRSETA5-1, pTHAN5-1, pRSETC5-2, pRSETBB1, pRESTCB1 and pRSETB-H3. Expression of the NS5 proteins was achieved by inducing the promoter with isopropyl-thio-${\beta}$-D-galactoside (IPTG) and confirmed by SDS-polyacrylamide gel electrophoresis. The NS5 proteins were immunoreactive against sera from Korean hepatitis C patients in Western blot analysis. Among the recombinant NS5 proteins, pRSETAS-1 plasmid derived protein, coded from aa2022 to aa2521 of HCV polyprotein, showed the strongest immunoreactivity against sera from Korean hepatitis C patients in immunoblot analysis. These results suggest that NS5 proteins would be useful as an antigen for detection of antibody against HCV in the blood samples.

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Layout optimization of wireless sensor networks for structural health monitoring

  • Jalsan, Khash-Erdene;Soman, Rohan N.;Flouri, Kallirroi;Kyriakides, Marios A.;Feltrin, Glauco;Onoufriou, Toula
    • Smart Structures and Systems
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    • v.14 no.1
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    • pp.39-54
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    • 2014
  • Node layout optimization of structural wireless systems is investigated as a means to prolong the network lifetime without, if possible, compromising information quality of the measurement data. The trade-off between these antagonistic objectives is studied within a multi-objective layout optimization framework. A Genetic Algorithm is adopted to obtain a set of Pareto-optimal solutions from which the end user can select the final layout. The information quality of the measurement data collected from a heterogeneous WSN is quantified from the placement quality indicators of strain and acceleration sensors. The network lifetime or equivalently the network energy consumption is estimated through WSN simulation that provides realistic results by capturing the dynamics of the wireless communication protocols. A layout optimization study of a monitoring system on the Great Belt Bridge is conducted to evaluate the proposed approach. The placement quality of strain gauges and accelerometers is obtained as a ratio of the Modal Clarity Index and Mode Shape Expansion values that are computed from a Finite Element model of the monitored bridge. To estimate the energy consumption of the WSN platform in a realistic scenario, we use a discrete-event simulator with stochastic communication models. Finally, we compare the optimization results with those obtained in a previous work where the network energy consumption is obtained via deterministic communication models.

Analyses of Flavor Qualities of Vegetable Oils by Gas Chromatography (가스크로마토그래피에 의한 식물성 기름의 향미품질의 분석)

  • Yoon, Suk-Hoo;Kim, Jong-Goon;Min, David B.
    • Korean Journal of Food Science and Technology
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    • v.17 no.5
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    • pp.355-360
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    • 1985
  • Soybean, hydrogenated soybean and corn oils, which were exposed to fluorescent light for different periods of time, were evaluated for sensory qualities by subjective sensory evaluation and instrumental gas chromatographic analysis. Sensory evaluation was conducted in 8 laboratories using a 10-point hedonic scale with a total of 95 panel members. The correlation coefficients between sensory scores and predicted sensory scores by gas chromatographic analysis for the 8 laboratories varied from 0.10 to 0.99. However, most laboratories had better than 0.90, which was considered excellent. The correlation coefficients between sensory scores of the 95 panel members and predicted sensory scores using the amount of 2. 4-decadienal isomers in oil determined by a gas chromatographic method for soybean, hydrogenated soybean, and corn oils, were r=0.96, r=0.97, and r=0.97, respectively. The correlation study suggests that it is possible to obtain realistic results of oil flavor qualities from the instrumental evaluation by combining improved gas chromatographic analysis, sensory evaluation, and statistical analysis for practical purposes.

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Selective Surface Oxidation of 590MPa TRIP Steel and Its Effect on Hot-Dip Galvanizability (590 MPa TRIP강의 선택적 표면산화 거동과 표면 산화막이 도금특성에 미치는 영향)

  • Kim, Seong-Hwan;Im, Jun-Mo;Huh, Joo-Youl;Lee, Suk-Kyu;Park, Rho-Bum;Kim, Jong-Sang
    • Korean Journal of Metals and Materials
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    • v.49 no.4
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    • pp.281-290
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    • 2011
  • In order to gain better understanding of the selective surface oxidation and its influence on the galvanizability of a transformation-induced plasticity (TRIP) assisted steel containing 1.5 wt.% Si and 1.6 wt.% Mn, a model experiment has been carried out by depositing Si and Mn (each with a nominal thickness of 10 nm) in either monolayers or bilayers on a low-alloy interstitial-free (IF) steel sheet. After intercritical annealing at $800^{\circ}C$ in a $N_2$ ambient with a dew point of $-40^{\circ}C$, the surface scale formed on 590 MPa TRIP steel exhibited a microstructure similar to that of the scale formed on the Mn/Si bilayer-coated IF steel, consisting of $Mn_{2}SiO_{4}$ particles embedded in an amorphous $SiO_{2}$ film. The present study results indicated that, during the intercritical annealing process of 590 MPa TRIP steel, surface segregation of Si occurs first to form an amorphous $SiO_{2}$ film, which in turn accelerates the out-diffusion of Mn to form more stable Mn-Si oxide particles on the steel surface. During hot-dip galvanizing, particulate $Fe_{3}O_{4}$, MnO, and Si-Mn oxides were reduced more readily by Al in a Zn bath than the amorphous $SiO_{2}$ film. Therefore, in order to improve the galvanizability of 590 TRIP steel, it is most desirable to minimize the surface segregation of Si during the intercritical annealing process.

Effect of Water-Containing Conditions on Concrete Substrates on Defects of Polyurethane-based Waterproofing Materials (콘크리트 바탕면의 함수조건이 폴리 우레탄계 방수재 하자에 미치는 영향)

  • Lee, Gun-Cheol;Kim, Jae-Yeob;Kim, Young-Min;Hong, Sung-Rok;Kim, Young-Sam;Shin, Hong-Chol
    • Journal of the Korea Institute of Building Construction
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    • v.23 no.1
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    • pp.1-9
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    • 2023
  • This study is to analyze the cause of the defects in polyurethane waterproofing, a material commonly used on the roof of buildings, and to determine if it has a relation with the curing conditions of humidity and temperature and the moisture content of the base structure. As a result, it was confirmed that the waterproofing coating did not adhere when the moisture content of the base plate was 10% or more. When the temperature and humidity conditions were 20℃ and 80%RH, none of the properties deteriorate but when the temperature was 40℃, 60%RH, air bubbles were formed on the surface, and at 40℃ and 80%RH, the basic properties of the dry coating film were less than the KS F 3211 performance standard.

A Study on the Creation and Maintenance of Laboratory Notebooks in Scientific Laboratories of a University (자연과학분야 대학실험실에서의 연구노트 작성 및 관리에 관한 연구)

  • Kim, Jihyun
    • Journal of Korean Society of Archives and Records Management
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    • v.11 no.1
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    • pp.139-159
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    • 2011
  • This study investigated the perceived significance of laboratory(lab) notebooks and the creation and maintenance of them from the perspectives of graduate students and professors in science fields. Semi-structured interviews of three professors and four graduate students were conducted. The interviewees were from three university laboratories of physics, chemistry and biological science. While professors perceived administrative continuity to be a reason for creating lab notebooks, students valued lab notebooks as a memory, and emphasized learning professional knowledge and skills through the recordkeeping practice. Students understood the ownership of lab notebooks and professors wanted to keep lab notebooks until they retire. Interviewees focused more on the content of lab notebooks rather than the form and the structure. They were also not willing to adopt an electronic lab notebook system. Students learned how to create lab notebooks through a course, their current or former academic advisors, seniors in the lab or well-written sample lab notebooks. In particular, students have learned how to create lab notebooks within their laboratories, which are considered to be communities of practice. In this sense, professors responsible for managing laboratories play an important part in teaching students the recordkeeping practice. University archives should also be more concerned with managing and preserving lab notebooks created from their universities.

Microstructures and Hardness of Al-Si Coated 11%Cr Ferritic Stainless Steel, 409L GTA Welds (Al-Si 용융도금된 11%Cr 페라이트 스테인리스강, STS409L GTA 용접부의 미세조직과 경도)

  • Park, Tae-Jun;Kong, Jong-Pan;Na, Hye-Sung;Kang, Chung-Yun;Uhm, Sang-Ho;Kim, Jeong-Kil;Woo, In-Su;Lee, Jong-Sub
    • Journal of Welding and Joining
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    • v.28 no.3
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    • pp.92-98
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    • 2010
  • Ferritic stainless steels, which have relatively small thermal expansion coefficient and excellent corrosion resistance, are increasingly being used in vehicle manufacturing, in order to increase the lifetime of exhaust manifold parts. But, there are limits on use because of the problem related to cosmetic resistance, corrosions of condensation and high temperature salt etc. So, Aluminum-coated stainless steel instead of ferritic stainless steel are utilized in these parts due to the improved properties. In this investigation, Al-8wt% Si alloy coated 409L ferritic stainless steel was used as the base metal during Gas Tungsten Arc(GTA) welding. The effects of coated layer on the microstructure and hardness were investigated. Full penetration was obtained, when the welding current was higher than 90A and the welding speed was lower than 0.52m/min. Grain size was the largest in fusion zone and decreased from near HAZ to base metal. As welding speed increased, grain size of fusion zone decreased, and there was no big change in HAZ. Hardness had a peak value in the fusion zone and decreased from the bond line to the base metal. The highest hardness in the fusion zone resulted from the fine re-precipitation of the coarse TiN and Ti(C, N) existed in the base metal during melting and solidification process and the presence of fine $Al_2O_3$ and $SiO_2$ formed by the migration of the elements, Al and Si, from the melted coating layer into the fusion zone.

Effect of Hydrogen Radicals for Ion Implanted CVD Diamond Using Remote Hydrogen Plasma Treatment(RHPT)

  • Won, Jaihyung;Hatta, Akimitsu;Yagi, Hiromasa;Wang, Chunlei;Jiang, Nan;Jeon, Hyeongmin;Deguehi, Masahiro;Kitabatake, Makoto;Ito, Toshimichi;Sasaki, Takatomo;Hiraki, Akio
    • The Korean Journal of Ceramics
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    • v.4 no.1
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    • pp.15-19
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    • 1998
  • Defects formation of Chemical Vapor Deposition (CVD) diamond on $^4He^{2+}$ irradiation and after remote hydrogen plasma treatment(RHPT) were investigated by cathodoluminescence(CL). As calculated in the TRIM simulation, the light elements of $^4He^{2-}$ can be penetrated into the diamond bulk structure at 3~4 $\mu\textrm{m}$ depth. The effects of the implantation region were observed when 5 keV~20 keV electron energy (insight 0.3~4.0$\mu\textrm{m}$) of CL measurement was irradiated to diamond at temperature 80 K. After the RHPT, rehybridization of irradiation damaged diamond was studied. The intensity of 5RL center(intrinsic defect of C) was diminished. The 2.16 eV center (N-V center) occurring usually by annealing could not be seen after RHPT. The diamond was rehybridized by hydrogen radicals without etching and thermal degradation by the RHPT.

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