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Antioxidation and Anti-inflammation Activity of Isodon inflexus (Thunb.) Kudo Extract and Its Isolated Substance (산박하 추출물과 분리물질의 항산화 및 항염증 활성)

  • Bu, Hee-Jung;Lee, Sunjoo
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.42 no.3
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    • pp.257-268
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    • 2016
  • The functions of anti-oxidation and anti-inflammation were investigated with the crude 80% methanol extract, subfractions and henryin isolated from Isodon inflexus (Thunb.) Kudo (I. inflexus (Thunb.) Kudo). Antioxidative ability was evaluated by bioassays using 2, 2-diphenyl-1-1-picrydrazyl (DPPH) free radical scavenging activity, xanthine oxidase inhibition, and superoxide radical scavenging effects. Ethyl acetate and butanol fractions exhibited free radical scavenging activity on superoxide with $IC_{50}$ values of $0.9{\mu}g/mL$, $0.2{\mu}g/mL$, respectively, which were stronger activity than that of allopurinol ($2.2{\mu}g/mL$) as reference. For the inhibition of anti-inflammatory activity in RAW 264.7 cell, the ethyl acetate fraction showed strong inhibition activity NO production, and henryin isolated from its subfraction reduced the activity in a dose-dependent manner. Ethyl acetate fraction and henryin suppressed not only mRNA expression of iNOS and COX-2, but also the mRNA expression of pre-inflammatory cytokines such as, TNF-${\alpha}$, 1L-$1{\beta}$, IL-6, in a dose-dependent manner. These results suggested that ethyl acetate fraction of I. inflexus (Thunb.) Kudo has considerable potential as a cosmetics ingredient with an antioxidant and anti-inflammatory effects and henryin can be applied as an functional reference.

Performance of CWDM Fabricated by the PLC-AWG Technology (평판형 AWG 기술을 이용한 광대역 파장다중화/역다중화 소자의 제작 및 특성)

  • Moon, H.M.;Kwak, S.C.;Hong, J.Y.;Lee, K.H.;Kim, D.H.;Kim, J.J.;Choi, S.Y.;Lee, J.G.;Lee, J.H.;Lim, K.G.;Kim, J.B.
    • Korean Journal of Optics and Photonics
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    • v.18 no.3
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    • pp.185-189
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    • 2007
  • A novel technology for CWDM (Coarse Wavelength Division Multiplexer) utilizing a PLC (Planar Lightwave Circuit)-AWG (Arrayed Waveguide Grating) fabrication process is proposed. BPM (Beam Propagation Method) Simulation results on the employed parabolic-horn-type input slab waveguide of AWG and the performance of the 20 nm-channel spacing CWDM with flattened passband are presented. Waveguides of $0.75{\triangle}%$ have been used in this experiment and the insertion loss at the peak wavelength is 3.5 dB for a Gaussian spectrum and is 4.8 dB for a flat-top spectrum. The bandwidth at 3 dB is better than 10 nm and 13 nm for Gaussian and flat-top spectra, respectively.

Earthworm Abundance and Species Composition in the Heap of Compost, Wild-grass and Sewer (퇴비더미, 야초더미와 농촌하수구에 서식하는 지렁이 종과 군집)

  • Na, Young-Eun;Hong, Yong;Lee, Sang-Beom;Koh, Mun-Hwan;Ahn, Young-Joon
    • The Korean Journal of Soil Zoology
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    • v.6 no.1_2
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    • pp.11-16
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    • 2001
  • This study was carried out to investigate the species composition and its abundance in the heap of compost, wild-grass, and sewer. 1,525 individuals were collected from 11th July, 2000 to 3rd August, 2000 at the 108 sites. They encompassed 3 families, 4 genera and 6-8 species. Species composition and its percentile abundance were as follows: Eisenia fetida 86.2%, Aporectodea trapezoides 0.5%, Amynthas hilgendorfi 1.1%, Amyntha agrestis 2.8%, A. koreanus group 6.3%, Drawida sp. 17%, Others 1.4fo in the compost heap, Eisenia fetida 9.8%, Aporectodea trapezoides 9.1%, Amynthas hilgendorfi 12.1%, Amyntha agrestis 25.1%, A. koreanus group 27.8%, Drawida sp 2.8%, Others 13.2% in the wild-grass and Eisenia fetida 24.2%, Aporectodea trapezoides 5.4%, Amynthas hilgendorfi 10.2%, Amyntha agrestis 5.4%, A. koreanus group 26.3%, Drawida sp 1.6%, Others 22.6% in the sewer.

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A Study of $C_9$-aldehyde Synthesis from n-Butene (노르말부텐으로부터 $C_9$-알데히드 합성에 관한 연구)

  • Jeon, Jong-Ki;Park, Seong-Ki;Park, Young-Kwon
    • Clean Technology
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    • v.14 no.3
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    • pp.176-183
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    • 2008
  • The purpose of this study is to upgrade the catalysts for synthesizing mixed octenes using normal butene and the catalysts for synthesizing $C_9$-aldehyde through hydroformylation of mixed octenes with syngas. The in-line activation method with circulating activating solution was effective for activation of the $NiO/A1_{2}O_3$ catalyst. The reason for catalyst deactivation may be ascribed to physi-sorbed materials or oligomers which block pore entrance and then prevent active sites from participating a reaction. Continuous distillation apparatus was used for separating mixed octenes from dimerization products. When reflux ratio was above 3 : 1, mixed octene fraction of which purity was above 99.57% was obtained. In $C_9$-aldehyde synthesis through hydroformylation of mixed octenes, we investigated a performance of ligand which increased catalyst stability as well as activity of Co catalyst. The results indicated that TPPO, NMP, NDMA, and succinonitrile were suitable ligand for increasing initial activity and reducing loss of Co during catalyst recovery.

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Periodic Mesh Generation for Composite Structures using Polyhedral Finite Elements (다면체 유한요소를 이용한 복합재 구조의 주기 격자망 생성)

  • Sohn, Dongwoo;Park, Jong Youn;Cho, Young-Sam;Lim, Jae Hyuk;Lee, Haengsoo
    • Journal of the Computational Structural Engineering Institute of Korea
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    • v.27 no.4
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    • pp.239-245
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    • 2014
  • Finite element modeling of composite structures may be cumbersome due to complex distributions of reinforcements. In this paper, an efficient scheme is proposed that can generate periodic meshes for the composite structures. Regular meshes with hexahedral finite elements are first prepared, and the elements are then trimmed to fit external surfaces of reinforcements in the composite structures. The trimmed hexahedral finite elements located at interfaces between the matrix and the reinforcements correspond to polyhedral finite elements, which allow an arbitrary number of nodes and faces in the elements. Because the trimming process is consistently conducted by means of consistent algorithms, the elements of the reinforcements are automatically compatible with those of the matrices. With the additional consideration of periodicity of reinforcements in a representative volume element(RVE), the proposed scheme provides periodic meshes in an efficient manner, which are compatible for each pair of periodic boundaries of the RVE. Therefore, periodic boundary conditions for the RVE are enforced straightforwardly. Numerical examples demonstrate the effectiveness of the proposed scheme for finite element modeling of complex composite structures.

The Optimal Enrichment Condition of Rotifer Brachionus rotundiformis (소화효소 활성으로 본 rotifer Brachionus rotundiformis의 적정 영양강화 조건)

  • Kwon, O-Nam;Park, Heum-Gi
    • Journal of Aquaculture
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    • v.21 no.1
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    • pp.41-46
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    • 2008
  • The purpose of the study was to suggest the optimal lipid enrichment conditions used digestive enzyme activity of rotifer changing due to water temperature and salinity. The high population growth appeared at the experiment temperature more than 28 degrees highly on the culture temperature(maximum 32 degrees, 1,453 individual/mL). The fecundity was low at high temperature, and the egg ratio was high at low temperature. Population growth of 10 and 15 ppt appeared in most highly, but the fecundity and the egg ratio were high most significantly appeared in natural seawater(32 psu). The digestive enzyme activity by the culture environment mainly showed high activity in natural seawater(amylase exclusion, 15 psu). However, the TAP activity by the water temperature showed highly at the more high temperature, but the amylase and the lipase appeared at low temperature. We carried out the lipid enrichment at 20 degrees and 26 degrees in a condition of the natural seawater. Total protein, the total essential amino acids differed not significantly. The methionine content that was essential amino acids, a total lipid content, unsaturated index of fatty acids, DHA and the DHA/EPA ratio were high significantly each in $20^{\circ}C$ enrichment trial. Therefore, we could suggest the $20^{\circ}C$ and natural seawater for the optimal lipid enrichment condition in aquaculture, because methionine contents, several indexes by the lipid, TG-lipase activity, fecundity and egg ratio are high.

Secondary Buckling Behavior Analysis on the Ship's Plate under Combined Load(Lateral Pressure Load and Axial Compressive Load) (조합하중을 받는 선체판부재의 2차좌굴거동 해석)

  • Park Joo-Shin;Ko Jae-Yong
    • Journal of the Korean Society of Marine Environment & Safety
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    • v.12 no.1 s.24
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    • pp.67-74
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    • 2006
  • The ship plating is generally subjected to combined in-plane load and lateral pressure loads. In-plane loads include axial load and edge shear, which are mainly induced by overall hull girder bending and torsion rf the vessel. Lateral pressure is due to water pressure and cargo. These load components are not always applied simultaneously, but more than one can normally exist and interact. Hence, for more rational and safe design rf ship structures, it is of crucial importance to better understand the interaction relationship of the buckling and ultimate strength for ship plating under combined loads. Actual ship plates are subjected to relatively small water pressure except for the impact load due to slamming and panting etc. The present paper describes an accurate and fast procedure for analyzing the elastic-plastic large deflection behavior up to the ultimate limit state of ship plates under combined loads. In this paper, the ultimate strength characteristics of plates under axial compressive loads and lateral pressure loads are investigated secondary buckling behavior through ANSYS elastic-plastic large deflection finite element analysis with varying lateral pressure load level.

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Temporal and Spatial Variations of Temperature and Salinity around Ganjeol Point in the Southeast Coast of Korea (한국 남동해 간절곶 주변해역의 열염구조와 시공간적 변동 특성)

  • Choo, Hyo-Sang;Jang, Duck-Jong
    • Journal of the Korean Society of Marine Environment & Safety
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    • v.20 no.5
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    • pp.474-485
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    • 2014
  • Temporal and spatial variations of temperature and salinity around Ganjeol Point during January, April, August and November 2011 were studied using the data from CTD observations and temperature monitoring buoys deployed at 20 stations in the southeast coast of Korea. Temperature and salinity were nearly homogeneous through the whole depth by mixing of the seawater in spring and winter related to the sea surface cooling. Stratification induced by the river runoff and the bottom cold water was clear in summer. In autumn, sea water had vertical mixing initiated from surface layer and weak stratification at the middle and bottom layers. Low temperature and high salinity emerged throughout the year near Ganjeol Point, which inferred from turbulent mixing and upwelling by its topographical effect. Major periods of 1/4~1.4 day temperature fluctuations were recorded for the most part of the stations. According to the cross spectral density analysis, high coherence and small time lag for temperature fluctuation between layers were shown at Ganjeol Point. However, those features at the northen area of Hoeya river were opposed to Ganjeol Point. From analyses, thermohaline structure and its fluctuation around Ganjeol Point were characterized into those three parts, the south of Ganjeol Point, Ganjeol Point and the north of Ganjeol Point.

Development of Fermented Beverage Using the Sea Tangle Extract, and Quality Characteristics Thereof (다시마추출액을 이용한 발효음료개발 및 품질특성)

  • Kim, Mi-Lim;Choi, Mi-Ae;Jeong, Ji-Suk
    • Food Science and Preservation
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    • v.15 no.1
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    • pp.21-29
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    • 2008
  • This study investigated the fermentation and quality characteristics of a fermented beverage, prepared by semi-anaerobic culture, using sea tangle extract. A central composit design using alcohol(0, 0.5, 1.0, 1.5, 2.0% [all v/v] ), sugar(0, 5, 10, 15, 20% [all w/v] ) and $65^{\circ}Brix$ citrus juice(0, 1.0, 2.0, 3.0, 4.0 % [all v/v] ) was used to find the optimal mix for fermentation. Sensory characteristics, such as color, flavor, taste, sweetness, saltiness, sourness and overall quality, were measured using a response surface methodology computer program. The optimal conditions that produced the highest acidity of 0.94 were 2.0 % ethanol, 10.17 % sucrose and 1.99 % citrus juice. The optimal conditions that produced gel 20.13 nun in thickness were 1.98% alcohol, 10.94% sucrose and 1.62% citrus juice. The overall optimal conditions that satisfied all the sensory requirements for a sea tangle beverage were 1.0% alcohol, 10.0% sucrose and 4.05% citrus juice.

Antioxidative and Fibrinolytic Activity of Extracts from Soybean and Chungkukjang(Fermented Soybeans) Prepared from a Black Soybean Cultivar (검은콩의 품종에 따른 콩과 청국장 추출물의 항산화능 및 혈전용해능)

  • Joo, Eun-Young;Park, Chan-Sung
    • Food Science and Preservation
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    • v.17 no.6
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    • pp.874-880
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    • 2010
  • We sought to develop natural preservatives or functional health foods from black soybeans (Yakkong, Seoritae and Chungkukjang). These materials were extracted with distilled water or 70% (v/v) ethanol, and the extracts sanalyzed for polyphenol content and tested for antioxidative and fibrilytic activities. The polyphenol contents of water extracts from Yakkong and Yakkong Chungkukjang was 316.23 mg/100 g, 896.01 mg/100 g, respectively, whereas those from Seoritae and Seoritae Chungkukjang were 304.28 mg/100 g and 875.23 mg/100 g. The polyphenol contents of water extracts from Chungkukjang were 2.8-fold higher than those from black soybean. The electron-donating abilities (EDAs) and superoxide dismutase (SOD)-like activities of water extracts from black soybean Chungkukjang were higher than those of water extracts from black soybean, but both extracts had fibrinolytic activity, the highest activity was present in water extracts of Yakkong Chungkukjang. The polyphenol contents, and antioxidative and fibrinolytic activities of extracts from black soybean Chungkukjang were higher than those of other black soybean extracts from black soybeans. These results indicate that black soybean Chungkukjang can be used for development of a health food or as a natural antioxidant.