Journal of Aquaculture (한국양식학회지)

The Korean Society of Fisheries and Aquatic Science (한국양식학회)
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The Optimal Enrichment Condition of Rotifer Brachionus rotundiformis

소화효소 활성으로 본 rotifer Brachionus rotundiformis의 적정 영양강화 조건

  • Kwon, O-Nam (Division of Marine Biosciences, Graduate School of Fisheries Science, Hokkaido University) ;
  • Park, Heum-Gi (Faculty of Marine Bioscience and Technology, Kangnung National University)
  • 권오남 (북해도대학 대학원수산과학연구원) ;
  • 박흠기 (강릉대학교 해양생명공학부)
  • Published : 2008.02.25
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Abstract

The purpose of the study was to suggest the optimal lipid enrichment conditions used digestive enzyme activity of rotifer changing due to water temperature and salinity. The high population growth appeared at the experiment temperature more than 28 degrees highly on the culture temperature(maximum 32 degrees, 1,453 individual/mL). The fecundity was low at high temperature, and the egg ratio was high at low temperature. Population growth of 10 and 15 ppt appeared in most highly, but the fecundity and the egg ratio were high most significantly appeared in natural seawater(32 psu). The digestive enzyme activity by the culture environment mainly showed high activity in natural seawater(amylase exclusion, 15 psu). However, the TAP activity by the water temperature showed highly at the more high temperature, but the amylase and the lipase appeared at low temperature. We carried out the lipid enrichment at 20 degrees and 26 degrees in a condition of the natural seawater. Total protein, the total essential amino acids differed not significantly. The methionine content that was essential amino acids, a total lipid content, unsaturated index of fatty acids, DHA and the DHA/EPA ratio were high significantly each in $20^{\circ}C$ enrichment trial. Therefore, we could suggest the $20^{\circ}C$ and natural seawater for the optimal lipid enrichment condition in aquaculture, because methionine contents, several indexes by the lipid, TG-lipase activity, fecundity and egg ratio are high.

본 연구의 목적은 rotifer Brachionus rotundiformis의 수온과 염분농도의 변화에 따른 이들의 소화효소 활성을 파악하고, 소화효소 활성을 기초로 적정 영양강화 조건을 찾는 것이다. 다른 온도에서 24시간 배양된 rotifer 밀도는 $32^{\circ}C$에서 1,453 개체/mL로 가장 높았다(P<0.05). 그리고 배양온도 $28^{\circ}C$에서 염분농도에 따른 rotifer 밀도는 15 에서 2,147 개체/mL로 가장 높게 나타났다(P<0.05). 배양환경에 따른 rotifer의 소화효소 활성에서 TAP 개체당 활성은 $24-32^{\circ}C$에서 유의적으로 높게 나타났으며(P<0.05), 단백질 비활성은 $32-36^{\circ}C$에서 높게 조사되었다(P<0.05). 그리고 TG-lipase 활성에서는 개체당 활성과 단백질 비활성 모두 $20^{\circ}C$에서 가장 높은 활성을 보였다(P<0.05). 또한 염분별 실험에서 TAP와 TG-lipase 활성은 32 psu에서 가장 높은 활성을 보였다(P<0.05). 32 psu에서 온도별 영양강화한 rotifer의 methionine 함량(% in protein), 지질 함량과 지방산 불포화도이 $20^{\circ}C$에서 유의적으로 높게 나타났다. 특히, DHA와 DHA/EPA 비가 $20^{\circ}C$ 실험구에서 12.6%와 12.5의 비로 각각 높았다(P<0.05). 따라서 rotifer B. rotundiformis의 지질 영양강화는 특히 TGlipase 활성을 높여 줄 수 있는 32 psu, $20^{\circ}C$에서 실시하는 것이 지방산 불포화도, DHA 및 DHA/EPA 비를 높여 줄 수 있는 효과적인 방법이라고 판단된다.

Keywords

References

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