• Hwankyungkyoyuk
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• v.19 no.3
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• pp.20-39
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• 2006

The purpose of the study is to develop e-Learning contents of environmental education for middle school students. The contents were developed based on literature review, the analysis of domestic and foreign programs and e-Learning contents on environmental education and expert advice. There are five main characteristics. First, this program has 12 sessions and is divided into six parts: introduction, air, water, animals and plants, soil, and resources and energy. Second, the contents were developed with consideration of middle school students' consist of optimized for learners by analyzing the needs of middle school students. Third, each part consists of five steps: "open", "unfold", "foster", "strengthen & refine", and "addition." Fourth, the contents have been developed in line with SCORM, an international standard for distnt education, to ensure reusability, accessibility, interoperability, and durability. Fifth, the contents are developed for blending online and off-line education. Developed program evaluated by 8 environmental education experts according to 10 evaluation item of total contents and contents development structure. In result, it evaluated comparatively high score.

• Proceedings of the Korean Biophysical Society Conference
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• 2003.06a
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• pp.34-34
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• 2003

Protein unfolding is a key step in several cellular processes, including protein translocation across some membranes and protein degradation by ATP-dependent proteases. C1pAP protease and the proteasome can actively unfold proteins in a process that hydrolyzes ATP, These proteases catalyze unfolding by processively unraveling their substrates from the attachment point of the degradation signal. As a consequence, the ability of a protein to be degraded depends on its structure as well as its stability. An ${\alpha}$-helix is easier to unravel than a ${\beta}$-strand. In multidomain proteins, independently stable domains are unfolded sequentially. The steric constraints imposed on substrate proteins during their degradation by the proteasome were investigated by constructing a model protein in which specific parts of the polypeptide chain were covalently connected through disulfide bridges. The cross-linked model proteins were fully degraded by the proteasome, but two or more cross-links retarded the degradation slightly. Our results suggest that the pore of the proteasome allows the concurrent passage of at least three stretches of a polypeptide chain, and also explain the limited degradation by the proteasome that occurs in the processing of the transcription factor NF-KB, and also implicate difficulty in degradation of amyloidal aggregates by the proteasome

• BMB Reports
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• v.35 no.2
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• pp.143-154
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• 2002

The structural and functional aspects of ervatamin B were studied in solution. Ervatamin B belongs to the $\alpha+\beta$ class of proteins. The intrinsic fluorescence emission maximum of the enzyme was at 350 nm under neutral conditions, and at 355 nm under denaturing conditions. Between pH 1.0-2.5 the enzyme exists in a partially unfolded state with minimum or no tertiary structure, and no proteolytic activity. At still lower pH, the enzyme regains substantial secondary structure, which is predominantly $\beta$-sheet conformation and shows a strong binding to 8-anilino-1-napthalene-sulfonic acid (ANS). In the presence of salt, the enzyme attains a similar state directly from the native state. Under neutral conditions, the enzyme was stable in urea, while the guanidine hydrochloride (GuHCl) induced equilibrium unfolding was cooperative. The GuHCl induced unfolding transition curves at pH 3.0 and 4.0 were non-coincidental, indicating the presence of intermediates in the unfolding pathway. This was substantiated by strong ANS binding that was observed at low concentrations of GuHCl at both pH 3.0 and 4.0. The urea induced transition curves at pH 3.0 were, however, coincidental, but non-cooperative. This indicates that the different structural units of the enzyme unfold in steps through intermediates. This observation is further supported by two emission maxima in ANS binding assay during urea denaturation. Hence, denaturant induced equilibrium unfolding pathway of ervatamin B, which differs from the acid induced unfolding pathway, is not a simple two-state transition but involves intermediates which probably accumulate at different stages of protein folding and hence adds a new dimension to the unfolding pathway of plant proteases of the papain superfamily.

• Journal of the Korea Safety Management & Science
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• v.23 no.3
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• pp.115-122
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• 2021

In this study, the purpose of this study was to examine the effect of twisting in the preparation of waterproofing in the process of unfolded donut-type staking method fire hoses in indoor hydrant system. The central pull-out method caused more twisting than the rolling method, and there was no significant difference in the number of twists according to the pull-out method in the case of male and female students. It was found that the time it took to untwist and prepare waterproofing was much shorter for male students. The angle valve and hose are connected, and the time to untwist and prepare for waterproofing after withdrawing the fire hose with the hose and nozzle connected was shorter than the unconnected state. In the rolling method, when a hose connected with two 15 m fire hoses was used and the angle valve-hose was connected, but the hose-nozzle was not connected, the least kinking occurred. The time to untwist and prepare for waterproofing was also the shortest. As a result, in the withdrawal method of the donut-type loaded fire hose in the indoor hydrant system, it is a rolling method rather than a central withdrawal method. With the angle valve and hose connected, unfold the fire hose with the hose and nozzle connected, if a large number of people unwind the twisted hose, the time to prepare for waterproofing can be shortened.

• Journal of the Architectural Institute of Korea Planning & Design
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• v.34 no.10
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• pp.83-94
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• 2018

In many theoretical and empirical studies on the design issues of therapeutic healthcare facilities, spatial configuration that promotes users' wayfinding behavior, has been emphasized as a significant factor to mitigate stressful experiences and to enhance restorative quality in the healthcare environment. This is also applicable to the healthcare setting for children. However, not much evidence has been reported with regard to the relationship between spatial configuration and wayfinding behaviors in this specific setting. Moreover, healthcare facilities for children with physical disabilities need more attention to provide easy wayfinding due to various physical restrictions. The aim of this study is therefore, to unfold the relationship between spatial configuration and visual cognitive qualities of outpatient spaces in the selected children's rehabilitation hospitals in Seoul, by examining visual cognitive attributes such as visibility, accessibility, and intelligibility. In the first phase, the spatial layout of the hospitals was analyzed, with an emphasis on the major outpatient areas such as the entrance lobby, doctors' examination, and physical therapy zones. In the second phase, a space syntax tool was implemented to examine visual cognitive characteristics of the spatial configuration. The spatial configuration parameters measured were integration, integration core, visual isovist field continuity, correlation between integration and step depth, and the correlation between integration n and integration 3. As a result, the integration was higher in the hall type configuration. Circulation intersections acted mostly as integration cores for better visibility. Some areas showed the lack of continuity in the visual isovist fields overlap and irregular correlation between integration and step depth. The intelligibility was higher in the circulation area and social interaction spaces such as a cafe, reception waiting, and therapy waiting areas. Based on the analysis, design implication and possible future improvement were discussed to enhance wayfinding experiences in the hospitals for children with physical disabilities.

• The Journal of the Convergence on Culture Technology
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• v.8 no.6
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• pp.175-184
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• 2022

Travel YouTubers who cross the boundary between tradition and novelty communicate by sharing their activities with others through SNS-based media. Media content should not only satisfy individuals, nor should it be too purpose-oriented. YouTube channels should be operated so that users can easily access content naturally, and more diverse methods can be pursued based on usage patterns and satisfaction theory. This study is about the type of perception of Generation Z on travel YouTube titles. As a result of conducting QUANL program analysis on 34 Q samples and 28 P samples from the Q methodological perspective, a total of three types were found. For types with unique characteristics, the first type was named "attention of keywords that draw imagination," the second type was "preferred to stories that stimulate curiosity," and the third type was "image satisfaction reflecting expectations." In addition, considering the characteristics of each type found, the scalability and strategic plan of the activities that Generation Z travel YouTubers want to unfold were presented.

• Design Convergence Study
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• v.14 no.4
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• pp.193-208
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• 2015

This study is to search a meaning of treagedy to show by the 6th generation through charecter's tragedy in the 6'th Generation Chinese film. For this, it examined an inner characteristic behind of the tragedy's moment and externalized ending in directors' films of the 6'th Generation's representation. The 6'th Generation of Chinese film reorganized newly Chinese film's cultural topography in the 1990s. The 6'th Generation films, which rebelled against the primary melody and the 5'th Generation on the system, is expressed commonly a tragedy moment with the time and space by character's tragedy. Characters of the 6'th Generation in the tragedy moment attempts various efforts to escape from the tragedy moment. However, no one can overcome the wall of reality, and their concealed emotion as keeping it out of the public eye lead that play. The moment that the 6th generation film meets a tragedy, the main character's accumulated tragedy situations begin to unfold all together. A tragedy embodied through : a collapsed tragedy due to the past's wound, a tragedy due to loss of jobs, a tragedy due to a family's departure, a tragedy from a time of chaos, a tragedy of a woman who became a head of a household and other tragedies.

• Journal of English Language & Literature
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• v.53 no.2
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• pp.243-260
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• 2007

This article explores how Richard Powers' The Gold Bug Variations, an interdisciplinary novel through the new concepts of biocriticism and bioliterature is connected with literature/art and science/technology. Powers uses Edgar Allen Poe's "The Gold Bug" and Johann Sebastian Bach's "The Goldberg Variations" for decoding DNA in order to analogize a genomic metaphor. He imagines literature as "the book of life" genome, written by DNA code due to the complexity and multiplicity of the genome. His novel, as 'genomic narrative,' shows the articulation of the genomic reading, and expression in the life language through the discourses of the information technology and the rhetorical tropes in biology. New biological ideas are continually required to articulate these processes. In the present tendency of the Human Genome Project, such advanced devices as biocybernetics offer the potential to open up new possibilities to researching the complexity of the genome. This can only happen if the following two ideas are followed: One is to comply with advanced technologies for processing the rapidly increasing data of the genome sequence; The other is to admit the necessary paradigm shift in biology. As shown above, the complexity and multiplicity of the genomic reality is not so simple. We must go beyond determinism, even if representation of a biological reality reveals the possibility of expressing its constituent elements by the advanced biotechnology. Consequently, in the unstoppable advances of the art of decoding the genome, The Gold Bug Variations interrelates to the interdisciplinary approaches through the rhetorical tropes that unfold the complex discursive world of the genome. Powers shows that the complex mechanisms of the genome in the microworld of every cell as the plot of "the book of life" can be designed and written using DNA language. At the same time, his genomic reading and writing demonstrate the historical processes of the shifting center of new genomic development and polysemous interpretation.

• Journal of Korean Medical classics
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• v.36 no.4
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• pp.93-108
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• 2023

Objectives : This paper aims to study the characteristics of zhongfeng treatment by examining the eight principles of zhongfeng treatment in the Zhongfeng Jiaoquan of Zhang Shanlei along with Zhang Bolong's treatment of 'Yangxu Leizhongfeng[Yang deficiency pseudo Wind damage]' which is missing from the eight principles. Methods : The treatment methods in the Zhongfeng Jiaoquan was organized in the order of cause, characteristic, symptom, treatment, and precautions, in order to analyze features that were emphasized by Zhang in zhongfeng treatment. Results : First, treatment for bizheng is to 'open and close', then apply methods of 'qianyang jiangqi(潛陽降氣)' and 'zhenni huatan(鎭逆化痰)' while that for tuozheng is to 'lianyin yiye(戀陰益液)' accompanied by medicinals that 'qianzhen xutang(潛鎭虛陽)'. Second, treatment for ganyang shangnizheng is to 'qianzhen rougan', while for tanzian yongsezheng, one must 'dangdi(蕩滌)' for those who are strong in qi, 'xiehua(泄化)' for those who are weak in qi, while for those who have qinizheng[qi reverse syndrome] to 'shunqi(順氣).' Third, for deficiency in xinye and ganyin, one must 'yuyin yangxue[育陰養血]', while for deficiency in shenyin, one must first 'qianjiang shena[潛降攝納]' then slowly apply the method of 'ziyang shenyin[滋養腎陰]' if there is no phlegm turbidity. Fourth, in order to communicate the meridians and unfold collaterals, if the pathogen is external, apply the method of 'yangxue tongluo[養血通絡]', while if the pathogen is internal, calm by doing 'qianyang zhenni[潛陽鎭逆].' Fifth, in order to treat pseudo zhongfeng caused by yang deficiency, one must 'lianyin gutuo[戀陰固脫]' while using medicinals that 'jiangxiang[潛降]'. Conclusions : Treatment of zhongfeng in the Zhongfeng Jiaoquan diverged from 'wenjing sanhan', the usual approach to zhongfeng which sees it as external, and established the 'qianjiang zhenshe [潛降鎭攝]' treatment method based on the internal wind theory. It suggests a new Korean Medical pathology based on theories of Western medicine, and introduces eight principles in treating zhongfeng, which would influence the treatment of zhongfeng in the future.

• International Journal of Advanced Culture Technology
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• v.11 no.4
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• pp.310-316
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• 2023

This study aims to predict uncertain future scenarios that may unfold in South Korea in the near future, utilizing the theory of extreme events(X-events). A group of 32 experts, consisting of government emergency planning officers, was selected as the focus group to achieve this objective. Using the Focus Group Interview (FGI) technique, opinions were gathered from this focus group regarding potential X-events that may occur within the advanced science and technology domains over the next 10 years. The analysis of these opinions revealed that government emergency planning officers regarded the "Obsolescence of current technology and systems," particularly in the context of cyber network paralysis as the most plausible X-event within science and technology. They also put forth challenging and intricate opinions, including the emergence of new weapon systems and ethical concerns associated with artificial intelligence (AI). Given that X-events are more likely to emerge in unanticipated areas rather than those that are widely predicted, the results obtained from this study carry significant importance. However, it's important to note that this study is grounded in a limited group of experts, highlighting the necessity for subsequent research involving a more extensive group of experts. This research seeks to stimulate studies on extreme events at a national level and contribute to the preparation for future X-event predictions and strategies for addressing them.