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Distribution of Mast cells and Nerves in the Developing Postnatal Submandibular and Sublingual Glands of Rats (생후 발생중인 흰쥐의 턱밑샘 및 혀밑샘에서의 비만세포와 신경의 분포양상)

  • Kim, Jae-Gon;An, Soo-Hyeon;Lee, Young-Su;Baik, Byeong-Ju;Cho, Eui-Sic
    • Journal of the korean academy of Pediatric Dentistry
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    • v.26 no.2
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    • pp.350-364
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    • 1999
  • The distribution of mast cells and nerves were investigated in the submandibular and sublingual glands of postnatal rats, using morphometric, histochemical and immunohistochemical techniques. Mast cells were observed in the submandibular and sublingual glands of postnatal development. Number of mast cells gradually increased in both glands following development. At birth, mast cells were relatively fewer in submandibular gland than those in sublingual gland, and they were mainly distributed in parenchymal tissues. At $2{\sim}4$ weeks, most of the mast cells were observed in the connective tissues, surrounding neurovascular elements, but some mast cells were closely related with the acini of submandibular gland. PGP 9.5 immunoreactive nerve fibers were found in the submandibular and sublingual glands of all developmental age. The nerve fibers were showed in varicose shape, and mainly located in adjacent area of ducts and vascular components of both glands. The number of nerve fibers were increased rapidly until 8 weeks, but they were not increased any more until 24 weeks. Therefore, it is suggested that mast cells and nerve fibers related with each other, and that their interactions may play roles not only in maturation of secretory units but also growth and differentiation of the tubular structures of the rat submandibular and sublingual glands during postnatal development.

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Geoacoustic characteristics of Quaternary stratigraphic sequences in the mid-eastern Yellow Sea (황해 중동부 제4기 퇴적층의 지음향 특성)

  • Jin, Jae-Hwa;Jang, Seong-Hyeong;Kim, Seong-Pil;Kim, Hyeon-Tae;Lee, Chi-Won;Chang, Jeong-Hae;Choi, Jin-Hyeok;Ryang, Woo-Heon
    • The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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    • v.6 no.2
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    • pp.81-92
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    • 2001
  • According to analyses of high-resolution seismic profiles (air gun, sparker, and SBP) and a deep-drill core(YSDP 105) in the mid-eastern Yellow Sea, stratigraphic and geoacoustic models have been established and seismo-acoustic modeling has been fulfilled using ray tracing of finite element method. Stratigraphic model reflects seismo-, litho-, and chrono-stratigraphic sequences formed under a significant influence of Quaternary glacio-eustatic sea-level fluctuations. Each sequence consists of terrestrial to very-shallow-marine coarse-grained lowstand systems tract and tidal fine-grained transgressive to highstand systems tract. Based on mean grain-size data (121 samples) of the drill core, bulk density and P-wave velocity of depositional units have been inferred and extrapolated down to a depth of the recovery using the Hamilton's regression equations. As goo-acoustic parameters, the 121 pairs of bulk density and P-wave velocity have been averaged on each unit of the stratigraphic model. As a result of computer ray-tracing simulation of the subsurface strata, we have found that there are complex ray paths and many acoustic-shadow zones owing to the presence of irregular layer boundaries and low-velocity layers.

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Factors Controlling Some Physicochemical Properties of Bentonite (벤토나이트의 물리-화학적 성질을 지배하는 요인분석)

  • 고상모;손병국;송민섭;박성환;이석훈
    • Journal of the Mineralogical Society of Korea
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    • v.15 no.4
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    • pp.259-272
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    • 2002
  • This study was tried to interpret the important major factors controlling some physicochemical properties by comparing mineralogical and physicochemical characteristics such as pH, cation exchange capacity, Methylene Blue adsorption amount, swelling, viscosity, strength (compressional and tensile), and surface area etc. Investigated bentonite samples are five Korean samples from Dusan, Naa, Oksan, Dongyang, and Yeonil deposits and two Japanese bentonites from Tsukinuno and Tomioka deposits which were formed under a similar geological environment of the Tertiary basin. Tsukinuno bentonite is only natural Na-type bentonite and the others are all Ca-type bentonites. Most of the properties are not explained by the montmorillonite content only though the most important factor controlling the physicochemical properties is the montmorillonite content. The layer charge of montmorillonite will strongly control cation exchange capacity and Methylene Blue adsorption. Zeolite bearing bentonites show the strong alkaline character and causes the increase of cation exchange capacity, however decrease swelling, viscosity and strengths. Pyrite bearing bentonites decrease green compressional strength and wet tensile strength. The exchangeable interlayer cations control some physicochemical properties. Na-type bentonite than Ca-type shows more strong alkaline character and much more advanced swelling and viscosity. Also the size and thickness of montmorillonite flakes seem to control some physicochemical properties. Bentonite mainly composed of montmorillonite of very thin and large flakes is characterized by the very high surface area, cation exchange capacity, viscosity, swelling, Methylene Blue adsorption, green compressional strength and wet tensile strength. Domestic Dusan bentonite shows the most excellent physicochemical properties, which is due to the high content(84%) and very well crystallinity of montmorillonite.

Effect of Dietary Supplementation of Fresh Water Algae Euglena on the Performance and Egg Quality and Fatty Acid Composition of Egg Yolk in Laying Hens (산란계에서 담수녹조류 Euglena의 첨가사료가 생산성 및 계란의 품질과 지방산 조성에 미치는 영향)

  • Choi S. W.;Park I. K.;Park B. S.
    • Korean Journal of Poultry Science
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    • v.31 no.4
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    • pp.283-291
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    • 2004
  • Feeding trials were conducted with Euglena strains grown under different media. The effect of supplementation of Euglena on the laying performance, egg quality and fatty acid composition of egg yolk was studied. In experiment I, two hundred eighty 32-wk-old ISA Brown layers were randomly assigned to seven dietary treatments for 4 wks. Each treatment consisted of 4 replications with 10 birds each housed in two birds cages. Control diet was formulated to have $17\%$ CP and 2,750 kcal ME/kg. Euglena gracilis Z. (EG) was added to control diet at the level of 0.25, 0.5, $1.0\%$ and Euglena gracilis Z. bleached and DHA enriched (EGBD; a strain mutated by streptomycin and cultivated in DHA enriched medium) at the level of 0.5, 1.0, $2.0\%$ in the diet. In experiment 2, three hundred 84-wk-old ISA brown layers were randomly assigned to five dietary treatments: T1; Control, T2; T1 + EGBD $0.5\%$, T3; T1 + Euglena gracilis Z. DHA enriched (EGD; cultivated in DHA enriched medium) $0.5\%$, T4; T1 + EGD $1.0\%$, T5; T1 + EGD $2.0\%$. Each treatment had 5 replication of 12 birds each housed in two birds cages. In experiments 1 and 2, Euglena suppplementation did not significantly affect egg production but increased egg weight and feed intake. In experiment 1, EG was more effective in increasing egg yolk color score than EGBD. Egg yolk color of EG $1\%$ treatment showed the highest score. EGBD supplementation increased DHA concentration of egg yolk. EGBD $2\%$ treatment showed the highest DHA and the lowest palmitic and stearic acids concentration in the egg yolk. In experiment 2, EGBD $0.5\%$ treatment showed highest DHA level in egg yolk (P<0.05). It was conducted that EGBD is a single cell protein source rich in DHA, that can be used to produce DHA enriched eggs.

Analysis of Sensitivity to Prediction of Particulate Matters and Related Meteorological Fields Using the WRF-Chem Model during Asian Dust Episode Days (황사 발생 기간 동안 WRF-Chem 모델을 이용한 미세먼지 예측과 관련 기상장에 대한 민감도 분석)

  • Moon, Yun Seob;Koo, Youn Seo;Jung, Ok Jin
    • Journal of the Korean earth science society
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    • v.35 no.1
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    • pp.1-18
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    • 2014
  • The purpose of this study was to analyze the sensitivity of meteorological fields and the variation of concentration of particulate matters (PMs) due to aerosol schemes and dust options within the WRF-Chem model to estimate Asian dusts affected on 29 May 2008 in the Korean peninsula. The anthropogenic emissions within the model were adopted by the $0.5^{\circ}{\pm}0.5^{\circ}$ RETRO of the global emissions, and the photolysis option was by Fast-J photolysis. Also, three scenarios such as the RADM2 chemical mechanism and MADE/SORGAM aerosol, the MOSAIC 8 section aerosol, and the GOCART dust erosion were simulated for calculating Asian dust emissions. As a result, the scenario of the RADM2 chemical mechanism & MADE/SORGAM aerosol depicted higher concentration than the others' in both Asian dusts and the background concentration of PMs. By comparing of the daily mean of PM10 measured at each air quality monitoring site in Seoul with the scenario results, the correlation coefficient was 0.67, and the root mean square error was $44{\mu}gm^{-3}$. In addition, the air temperature, the wind speed, the planetary boundary layer height, and the outgoing long-wave radiation were simulated under conditions of no chemical option with these three scenarios within the WRF or WRF-Chem model. Both the spatial distributions of the PBL height and the wind speed of u component among the meteorological factors were similar to those of the Asia dusts in range of 1,800-3,000 m and $2-16ms^{-1}$, respectively. And, it was shown that both scenarios of the RADM2 chemical mechanism and MADE/SORGAM aerosol and the GOCART dust erosion were interacted on-line between meteorological factors and Asian dusts or aerosols within the model because the outgoing long-wave radiation was changed to lower than the others.

A study for Beating Filter Press Dewatering Technology (열(熱) 필터프레스 기술(技術)을 통한 슬러지 탈수율(脫水率) 향상(向上)을 위한 연구(硏究))

  • Lee, Jung-Eun;Kim, Dong-Su
    • Resources Recycling
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    • v.15 no.3 s.71
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    • pp.38-45
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    • 2006
  • The thermal filter press dewatering(TFPD) technology to improve the dewaterability through increasing the inner vapor pressure, lowering the filtration viscosity and forming the porosity easily within cake as applying the heat at the sludge layer was developed in this study. The hot water with temperature of $95^{\circ}C$ and pressure of $1.2kg_f/cm^2$ was supplied to the heating plate equipped between filter plates with plate size of $470{\times}470mm$ and material of polypropylene. Sludge was dewaterd by supplying pressure of $5kg_f/cm^2$ and then by squeezing pressure of $15kg_f/cm^2$. As a results of estimating the characteristics of thermal dewatering to consider the initial water content and organic content to be influenced by a period of water shortage and rainwater, the dewatered cake water content was about 35 wt% and dewatering velocity was $4DSkg/m^2{\cdot}hr$ under the rainwater period, and the dewatered cake water content was about 50 wt% and dewatering velocity was $1.5DSkg/m^2{\cdot}hr$ in the case of sludge of water shortage season. These results was superior to the mechanical dewatering performance with water content of 70wt% and dewatering velocity of $0.9DSkg/m^2{\cdot}hr$. On the base of the results of TFPD, energy consumpted to deal with DS(Dry Solid) of 1kg was estimated by 300 kJ. It was analyzed that the energy consumption of TFPD was decreased about one third with comparison to the dryer system. Dewatering velocity of this technology was faster than the one of mechanical dewatering equipment and it was easier to product low water content cake. Therefore, this technology was recognized that dewaterability was predominant because of the fast of dewatering velocity and production of low water content cake, and also this known as economical efficiency was excellent because of low energy consumption in comparison with dryer.

Injury of Neurons by Oxygen-Glucose Deprivation in Organotypic Hippocampal Slice Culture (뇌 해마조직 절편 배양에서 산소와 당 박탈에 의한 뇌신경세포 손상)

  • Chung, David Chanwook;Hong, Kyung Sik;Kang, Jihui;Chang, Young Pyo
    • Clinical and Experimental Pediatrics
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    • v.51 no.10
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    • pp.1112-1117
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    • 2008
  • Purpose : We intended to observe cell death and apoptotic changes in neurons in organotypic hippocampal slice cultures following oxygen-glucose deprivation (OGD), using propidium iodide (PI) uptake, Fluoro-Jade (FJ) staining, TUNEL staining and immunofluorescent staining for caspase-3. Methods : The hippocampus of 7-day-old rats was cut into $350{\mu}m$ slices. The slices were cultured for 10 d (date in vitro, DIV 10) and and exposed to OGD for 60 min at DIV 10. They were then incubated for reperfusion under normoxic conditions for an additional 48 h. Fluorescence of PI uptake was observed at predetermined intervals, and the cell death percentage was recorded. At 24 h following OGD, the slices were Cryo-cut into $15{\mu}m$ thicknesses, and Fluoro-Jade staining, TUNEL staining, and immunofluorescence staining for caspase-3 were performed. Results : 1) PI uptake was restricted to the pyramidal cell layer and DG in the slices after OGD. The fluorescent intensities of PI increased from 6 to 48 h during the reperfusion stage. The cell death percentage significantly increased time-dependently in CA1 and DG following OGD (P<0.05). 2) At 24 h after OGD, many FJ positive cells were detected in CA1 and DG. Some neurons had distinct nuclei and processes while others had fragmented nuclei and disrupted processes in CA1. TUNEL and immunofluorescent staining for caspase-3 showed increased expression of TUNEL labeling and caspase-3 in CA1 and DG at 24 h after OGD. Conclusion : The numerous dead cells in the slice cultures after OGD tended to display apoptotic changes mediated by the activation of caspase-3.

A Study on the Frequency of Occurrence of the Aortic Dissection using CT (CT 검사에서 대동맥박리(aortic dissection)의 발생빈도에 관한 고찰)

  • Dong, Kyung-Rae;Choi, Sung-Kwan;Jang, Young-Ill;Ro, Sang-Ho
    • Journal of radiological science and technology
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    • v.31 no.2
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    • pp.115-121
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    • 2008
  • Purpose: Aortic Dissection is very dangerous, prognostic disease, which the bloodstream flow out of the true lumen of the aorta by the bursting of aortic intima resulting in a rapid dissociation of inner and outer layer from the media. It is difficult to diagnose aortic dissection clinically by normal X-ray. This study was to investigate the occurrence frequency by age and number of patients who are identified to be aortic dissection by CT (Computed Tomography) scan. Materials and methods: We investigated the trend of yearly fluctuation, gender, age, and department of clinical research of the 112 patients who conducted CT scan in C- University Hospital for two years from January 2005 to December 2006. The MIP and SSD which reconstructed CT image and the VRT image were obtained for the accurate observation. The result was investigated by comparing normal X-ray and CT scan. Results and Conclusion: 1. The yearly check of 112 patients conducted CT scan showed 37 people (41.9%) in 2005, and it was increased to 65 (58.1%) in 2006 by 1.4 times. 2. The gender distribution of patients given a CT scan showed 45 males (40.1%), and female 67 (59.9 %). The aortic dissection patients were 9 (20%) out of 45 males, 21 (31.3%) out of 67 females and women were 1.6 times more than men. Women are also 1.5 times more than men in the number of examinee. 3. The age distribution of patient's who conducted CT scan revealed that there was no patient under 30 years old while 88.3% of all patients were through 41 to 80 years old. The higher the age was, the higher the occurrence of aortic dissection was. The difference in the occurrence frequency of age was statistically significant (p<0.01). 4. The departments that requested CT scan were the emergency department 46 (41.1%), circulatory internal medicine 37 (33.0%), chest surgery 13 (11.6%), and others 6 (14.3%). The combined ratio of emergency medicine and circulatory internal medicine was 74.1% of all. The results show that the aortic dissection is a very dangerous disease whose patients visit mainly via the emergency room. 5. The aortic dissection patients had normal X-ray readings in 22 (73.3%) out of 30, and only 8 (26.7 percent) are abnormal in the X-ray diagnosis. Therefore, the CT scan needs to be enforced in order to assess accurately the disease of aortic dissection.

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Determination of Chimera Types and Ploidy Level of Sports from 'Campbell Early' Grape (Vitis labruscana) (포도 '캠벨얼리' 품종에서 발생한 아조변이체의 배수성 및 키메라 형태 검정)

  • Noh, Jung-Ho;Park, Kyo-Sun;Yun, Hae-Keun;Do, Gyung-Ran;Hur, Youn-Young;Kim, Seung-Hui;Lee, Han-Chan;Ryou, Myung-Sang;Park, Seo-Jun;Jung, Sung-Min
    • Horticultural Science & Technology
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    • v.28 no.6
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    • pp.996-1002
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    • 2010
  • Flow cytometry (FCM) was used to measure the ploidy level of three different sports from 'Campbell Early' ($Vitis$ $labruscana$) grape. Results of the study showed different ploidy levels. FCM analysis for 'Campbell Early' grape which contains 2C DNA diploid cells showed single peak around 35-40 while 'Kyoho' grape with 4C DNA tetraploid cells had a different level of 70-80. However, analysis of the sports displayed a histogram with 2 peaks containing both 2C and 4C nuclei. There was no difference in histograms of 2C DNA flesh and pericarp; on the other hand, 4C DNA flesh type of sports had a different histogram from that of the 2C DNA pericarp. Chromosome numbers of diploid ('Campbell Early'), tetraploid ('Kyoho'), and three sports were counted under the microscope. 'Campbell Early' and 'Kyoho' have 38 and 76 chromosomes, respectively. Three different sports are mixoploids with mixtures of diploid and tetraploid cells. Microscopic observations of shoot apical meristems in sports from 'Campbell Early' grape were carried out to determine the type of plant chimera. 'Campbell Early' grape (diploid) and 'Kyoho' grape (tetraploid) showed that both had 2 tunica layers covering corpus cells, while the three different sports had tunica layers showing mostly oblique division. Most cells from 'Kyoho' grape were larger than 'Campbell Early' grape. Cells from L-2 and L-3 layers of the three sports were similar to 'Kyoho' grape in size, although all cells in L-1 surface layer were uniform in size like 'Campbell Early' grape. Results of FCM analysis indicated that both normal and polyploid cells could be intermixed in sports and could become mixoploidy consisting of diploid and tetraploid. All sports used in the tests were periclinal chimera plants with two distinct L-1 and L-2 cell layers. The result of this study suggests that all three sports which originated from 'Campbell Early' grape might be 2-4-4 type chimera formation.

Director Oshii Mamoru's Recognition and Representation of Modern World Shown in (<스카이 크롤러>를 통해 본 오시이 마모루 감독의 '현대세계' 재현과 인식)

  • Moon, Jae-Cheol;Park, Nam-Ki
    • Cartoon and Animation Studies
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    • s.27
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    • pp.1-30
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    • 2012
  • Oshii Mamoru attempts to project the perception of reality about his own modern world as it is through animation. The precedent studies on Oshii Mamoru put their focus on the point that his works have a tendency to represent the devastation of reality as the image of 'a ruin.' In addition, they gave an impression that Oshii Mamoru's animation has gradually developed to expose the 'realistic taste[beauty]' from the design perspective. However, this study is going to put its focus on the point that Mamoru's works were created as an attempt at finding the subversive possibility of the suppressed modern world under the modern capitalism in the aspect of 'everyday life' of the modern world. First, for its analytical purpose, this study intends to do that by dividing the work into the three layers such as space, body and everyday life. In Chapter II, this study, in relation with the issue of 'space' representation, analyzed the space in into the modern, mechanical, compactly capitalized space and also the space just like a closed circuit having the nature of 'repetition.' In Chapter III, this analyzed that suggests the body moving freely between a human named 'Kildren' and the inhuman from the aspect of character's body, through which Mamoru represents the capitalized, reified body of the modern world. In addition, this analyzed the extreme anxiety facing the body into the reflection of the phenomena of 'anxiety' and 'placelessness' consequent on the labor flexibility of the modern world. In Chapter IV, this study, on the basis of the analysis of layers of space & body, analyzed that the 'everyday life' of the modern world was represented in the respects of 'memory' and 'habit', and in the aspects of Mise-en-Sc$\grave{e}$ne, design and direction. In Chapter V, this rooted out the fact that Mamoru suggested the attempt at 'appropriation' based on his perception of reality about such a modern world. Such a finding includes unearthing the fact that Oshii Mamoru's work is raising a question about how to desert and appropriate the modern space. In conclusion of Chapter VI, this drew the conclusion that Oshii Mamoru's work represented the layer of 'everyday life' while dealing with the post-modern themes shown by the existing modern SF genre, provided the 'window' through which people can perceive the 'modern world radically by recommending an attempt at 'appropriation', and blazed a trail in a new realm of creation for animations.