• 대한의생명과학회지
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• 제13권2호
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• pp.141-148
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• 2007

We determined the sequences of the quinolone resistance-determining region (QRDR) of gyrA and gyrB for 21 clinical strains of Enterobacteriaceae resistant to ciprofloxacin, norfloxacin and levofloxacin. The clinical strains were isolated from the specimens of three general hospitals in Busan. In the present study, we found mutations in type II topoisomerase (DNA gyrase) genes for all strains. We confirmed that some genera of Enterobacteriaceae of clinical specimen exhibited decreased sensitivity to fluroquinolone due to changes in Ser-83$\rightarrow$Leu and Asp-87$\rightarrow$Asn types on gyrA and alterations in Glu-465$\rightarrow$Arg and Ser-492$\rightarrow$Asn type on gyrB. All the twenty-one strains had a missense mutation in gyrA (codon 83 and 87). Three of them had an additional mutation in gyrB (codon 465 or 492), but one of them had an additional mutation in gyrB (codon 426, 427, 491, 495 and 496). The strains which had two mutations in type II topoisomerase genes (gyrA and gyrB) were significantly more resistant to fluoroquinolones than those with a single mutation in gyrA (mean MICs of ciprofloxacin: $\geq8\mu$g/ml, mean MICs of levofloxacin: $\geq16\mu$g/ml). Interestingly, the examination of silent nucleotide changes n the gyrA and gyrB genes revealed six different patterns of DNA polymorphism, respectively. Fifteen strains of the twenty-one strains bearing the gyrase A mutation shared the same polymorphism and eleven strains of the twenty-one strains bearing the gyrase B mutation shared the same polymorphism.

• 생약학회지
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• 제41권3호
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• pp.227-231
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• 2010

Protein tyrosine phosphatase 1B (PTP1B) is predicted to be therapeutic target in treatment of type 2 diabetes and obesity. Thus, in order to search for PTP1B inhibitors, we screened the inhibitory activity of PTP1B in the water extracts of 84 medicinal herbs. Among them, the extracts of Pini Folium, Magnoliae Cortex, Artemisiae asiaticae Herba, Schizonepetae Herba, Menthae Herba, Mume Fructus, Cimicifugae Rhizoma, and Amomi Cardamomi Fructus showed relatively significant (58-68%) inhibitory activity against PTP1B. Especially, the methylene chloride fraction of the methanol extract of Menthae Herba (81% inhibition at 30 ${\mu}g$/ml) showed more potent inhibitory activity against PTP1B than others.

• 생명과학회지
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• 제16권3호
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• pp.485-492
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• 2006

광합성세균인 Rhodobacter sphaeroides의 PrrBA two-component system은 산소분압의 변화에 따라 광합성 유전자의 발현을 조절하는 주요한 조절계 중 하나이다. PrrBA two-component system은 PrrB histidine kinase와 PrrA response regulator로 구성되어 있는데, PrrB의 N-말단 transmembrane 도메인은 신호인지 도메인으로서, 여섯 개의 transmembrane helix가 세 개의 periplasmic loop와 두 개의 cytoplasmic loop을 형성하고 있다. 그 중 세 번째, 네 번째 transmembrane helix와 두 번째 periplasmic loop가 산화/환원 인지 기능에 있어 중요한 역할을 할 것이라고 제안되었다. 본 연구에서는, 두 번째 periplasmic loop와 그 인접 부위에서의 돌연변이 (Asp-90, Gln-93, Leu-94, Leu-98, Asn-106)에 의해 PrrB의 인지 기능에 있어 심각한 결함이 생기는 것을 증명하였고, 이는 이 아미노산들이 PrrB의 산화/환원 인지 기능에 연관되어 있을 수 있다는 것을 보여준다. PrrB의 돌연변이 형태 (D90E, D90N, D90A)가 대장균에서 과발현되어서 affinity chromatography에 의해 정제되었고, 정제된 단백질의 자가인산화 반응이 측정되었다. PrrB D90N 변이형태는 PrrB wild-type보다 높은 자가인산화 활성을 가지는 반면에, PrrB D90E 변이형태는 PrrB wild-type보다 낮은 자가인산화 활성을 나타내었다. 그리고 D90A 변이형태는 PrrB의 자가인산화 활성이 매우 약화되었다.

• 식물조직배양학회지
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• 제21권1호
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• pp.59-63
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• 1994

세포유전적 유형이 다른 무릇 (Scilla scilloidise Coplex)의 조직배양에서 유도된 캘러스에서 염색체 변이를 조사하였다. AA유형의 캘러스에서 심한 수적, 구조적 변이가 관찰된 반면, BB 유형의 캘러스 세포에서는 상염색체의 변이가 발견되지 않았다. 이질 4배체인 AABB유형에서는 두개의 hypoploid 세포만 관찰되었으나, B게놈의 진정4배체인 BBBB유형에서는 hypoploid 세포와 함께 hyperploid 세포가 관찰되었다. 캘러스 세포에서 상염색체의 안정성은 B염색체를 지닌 식물에서 유도된 캘러스에서 더 높게 나타났다. 이는 배양세포에서 B염색체가 상염색체의 안정성에 선택적으로 기능하기 때문인 것으로 여겨진다.

• 한국전기전자재료학회:학술대회논문집
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• 한국전기전자재료학회 2001년도 추계학술대회 논문집 Vol.14 No.1
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• pp.414-417
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• 2001

Recently, developments of device using characteristics of ceramics as a new technical material is in progress. While doing so, Ultrasonic motor which is a part of research & development of piezo-actuator by piezo-effects is being used in the various applications. So, we fabricated a flat-type $L_{1}-B_{8}$ mode Ultrasonic motor and measured the operation characteristics of its. The size of USM is 80*20*1.5[$mm^{3}$](length*width*thickness) and is constructed with stator by piezo-ceramics and stainless elastic body and rotator by bearings. As results of experiments, the fastest speed of revolution(v), the maximum torque(T) and the efficiency( $\eta$ ) were 37.5[cm/sec], 5.0[ $mN{\cdot}m$ ] and 1.17[%] respectively when 27.9[kHz], 150[gf], 50[V] were applied. So, we think this $flat-type_{1}-B_{8}$ mode Ultrasonic motor is able to be used for applications in forwarding device of a paper or electric card and so on.

• Journal of Nutrition and Health
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• 제35권10호
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• pp.1045-1052
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• 2002

Hyperhomocysteinemia, resulted from an interaction between the mutation of MTHFR gene and B vitamin deficiency, is suggested as a possible cause for complications and adverse outcomes of pregnancy. The purpose of the present study was to investigate the relationship between the intakes of B vitamins and serum homocysteine concentrations with the C677T mutation in the MTHFR genotypes in 135 normal pregnant women of 24-28 weeks of gestation. Dietary intake of B vitamins did not differ among the three genotypes, but the negative correlation between dietary folate intake and the serum homocysteine level was the strongest in the T/T type (r ＝ -0.249) than in other genotypes (C/T: r＝ -0.040, C/C:r＝ 0.126, p＜0.05). Among the subject with the T/T type, the pregnant women who consumed folate less than 50% of the RDA had higher serum homocysteine levels than those who consumed folate greater than 125% of the RDA (10.4$\pm$5.9 vs 7.0$\pm$1.5 $\mu$mol/L, p＜0.05). Serum homocysteine levels were higher in the women with micronutrient supplements than those with no supplements in the T/T type, but such relation was not present in the C/C or the C/T type. In conclusion, serum homocysteine concentrations were influenced by the interrelationship between the MTHFR polymorphisms and dietary folate intake or micronutrient supplementation.

• Composites Research
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• 제15권4호
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• pp.17-22
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• 2002

미세 직경을 갖는 복합재료 나선형 스프링 (CS)가 광점퍼코드 (OJC)를 보강하기위한 매체로써 개발되었다. 이 스프링의 외경은 약 2~3mm로써 광점퍼코드에 갑작스런 측면 하중으로 부터 광섬유의 손실을 막기위해 삽입 보강할 수 있도록 제작되었다. 섬유 형태 (Y-type)와 밴드 형태 (B-type)의 복합재료 스프링이 제작되어 그 효과를 비교하였다. 측면 하중에 대한 기계적 특성은 동일 직경의 금속 스프링 및 일반 광점퍼코드의 물성치와 비교하여 제시하였다. 실험 결과로부터 복합재료 스프링이 보강된 광점퍼코드의 경우 굽힘에 대한 높은 저항력을 지니고 있음에 따라 광섬유의 내부 손상에 의한 광 손실의 감소률이 낮음을 알 수 있었다 얻어진 주요 결과들은: (1) Y-type의 CS의 경우 B-type과 비교하여 높은 측압 저항력을 지님을 알 수 있었다 (2) 일반 OJC와 비교하여 CS-OJC의 경우 광 손실이 현격이 낮음을 알 수 있었다. (3) 일반 스프링의 측압 하중시의 응력 분포 형태를 제시하였으며. 실험으로부터 얻어진 결과로부터 복합재료 스프링이 보강된 광점퍼 코드의 경우 매우 높은 구조적 안정성을 보임을 알 수 있었다.

• Journal of the Korean Wood Science and Technology
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• 제51권4호
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• pp.239-252
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• 2023

In this study, bending and compression strength tests were performed to investigate effect of composition of layer layout of Larix cross-laminated timber (CLT) on mechanical properties. The Larix CLT consists of five laminae, and specimens were classified into four types according to grade and composition of layer. The layer's layout were composited as follows 1) cross-laminating layers in major and minor direction (Type A), and 2) cross-laminating external layer in major direction and internal layer applied grade of layer in minor direction (Type B). E12 and E16 were used as grades of lamina for major direction layer of Type A and external layer of Type B according to KS F 3020. In results of the bending test of CLT using same grade layer according to layer composition, the modulus of elasticity (MOE) of Type B was higher than Type A. In case of prediction of bending MOE of Larix CLT, the experimental MOE was higher than 1.00 to 1.09 times for Shear analogy method and 1.14 to 1.25 times for Gamma method. Therefore, it is recommended to predict the bending MOE for Larix CLT by shear analogy method. Compression strength of CLT in accordance with layer composition was measured to be 2% and 9% higher for Type A using E12 and E16 layers than Type B, respectively. In failure mode of Type A, progress direction of failure generated under compression load was confirmed to transfer from major layer to minor layer by rolling shear or bonding line failure due to the middle lamina in major direction.

• 한국건축시공학회:학술대회논문집
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• 한국건축시공학회 2023년도 가을학술발표대회논문집
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• pp.109-110
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• 2023

Domestic ultra-hard road repair materials require a lot of time before the road can be opened to traffic. Therefore, in this study, Methyl Methacrylate (MMA) based road repair material was used to improve the above problems. Furthermore, the basic physical properties of MMA-based pavement repair materials are examined to confirm their suitability in concrete pavements. For this study, two types of MMA road repair materials (A type and B type) were selected. Then, the curing of the test specimens prepared for painting was carried out under three conditions. The experimental items were viscosity (drop time) and drying time (set to touch, dry-hard). As a result of the experiment, viscosity (drop time) was faster in type A than in type B. The drying time results were as follows. In the case of set ti touch, both type A and type B dried in about 10 minutes regardless of the curing conditions. In the case of dry-hard, regardless of the curing conditions, A type dried longer than B type, but it dried faster than conventional road repair materials. Therefore, within the scope of this study, it is considered that A type has a high potential for utilization as a road repair material.

• Clinical and Experimental Pediatrics
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• 제49권3호
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• pp.242-250
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• 2006

Haemophilus influenzae type b(Hib) conjugate vaccines prevent Hib disease in individuals and reduce the carriage and transmission of the organism in the community. The incidence of Hib disease has been decreased dramatically in a diverse range of countries through the use of a variety of conjugate vaccines and vaccine schedules. In some countries, the vaccine has caused a near-disappearance of invasive Hib disease through a combination of direct protection and herd immunity. The effectiveness of the vaccine was not modified by the type of conjugate vaccine, the number of doses given(two, three or four), age at first vaccination(two months, 42 to 90 days, three months) and whether the vaccine was tested in an industrialized or developing country. Over 15 years of international experience with vaccines has also demonstrated that they are safe. In 2004, Hib vaccines were adapted in routine immunization in 92 countries in the world. Decisions regarding the use of the Hib vaccine in routine immunization schedules depend not only on the effectiveness and efficacy of the vaccine but also on factors such as burden of disease, vaccine cost, and competing priorities. In Korea, Hib disease burden seemed to be lower than other developed countries(~10/100,000). Moreover Hib vaccines showed excellent immunogenicity in Korean children in many studies. Therefore, a potential approach to economize the cost of Hib vaccines could be to explore the possibilities of using reduced vaccine doses for immunization as some other countries.