• Journal of Microbiology and Biotechnology
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• v.17 no.5
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• pp.712-720
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• 2007

To investigate clonal variations of recombinant Chinese hamster ovary(rCHO) clones in response to culture pH and temperature, serum-free suspension cultures of two antibody-producing CHO clones(clones A and B), which were isolated from the same parental clone by the limiting dilution method, were performed in a bioreactor at pH values in the range of 6.8-7.6, and two different temperatures, $33^{\circ}C\;and\;37^{\circ}C$. In regard to cell growth, clone A and clone B displayed similar responses to temperature, although their degree of response differed. In contrast, clones A and B displayed different responses to temperature in regard to antibody production. In the case of clone A, no significant increase in maximum antibody concentration was achieved by lowering the culture temperature. The maximum antibody concentration obtained at $33^{\circ}C$(pH 7.4) and $37^{\circ}C$(pH 7.0) were $82.0{\pm}2.6$ and $73.2{\pm}4.1{\mu}g/ml$, respectively. On the other hand, in the case of clone B, an approximately 2.5-fold increase in maximum antibody concentration was achieved by lowering the culture temperature. The enhanced maximum antibody concentration of clone B at $33^{\circ}C$($132.6{\pm}14.9{\mu}g/ml$ at pH 7.2) was due to not only enhanced specific antibody productivity but also to prolonged culture longevity. At $33^{\circ}C$, the culture longevity of clone A also improved, but not as much as that of clone B. Taken together, CHO clones derived from the same parental clone displayed quite different responses to culture temperature and pH with regards antibody production, suggesting that environmental parameters such as temperature and pH should be optimized for each CHO clone.

• The Journal of Korean Obstetrics and Gynecology
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• v.21 no.3
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• pp.111-120
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• 2008

Purpose: Although premsnstrual syndromes(PMS) have long been recognized, there has been difficulty to evaluate the symtoms. Usually the questionnaire has been used to dignose the PMS. Objective is to investigate the relationship of body temperature between women with PMS and without PMS. Methods: We studied 23 patients visiting ㅇㅇ hospital from 26th December 2005 to 26th April 2006. The Questionnaire for PMS was used to evaluate physical, emotional, and behavioral symptoms accompanying the menstrual cycle of the subjects. The subjects were categorized in two groups, non-PMS(11) and PMS group(12). Body temperature was assessed by Dorex spectrum 9000MB (DOREX Inc.. USA). We measured CV4, CV3, CV12 and CV17 to evaluate the distribution of body temperature, compared the difference of temperature(${\Delta}T$) between CV17-CV4, CV17-CV3, CV17-CV12 and CV12-CV3. We investigated the of temperature and ${\Delta}T$ between two groups by Mann-Whitney U-test. Results: The temperature of CV3 and CV 4 of PMS located in low abdomen were lower than those of non-PMS located in chest. But there was no statistical significance of temperature between two groups. There was lower temperature of low abdomen in PMS group than non-PMS group without statistical significance. Conclusion: The results suggest that DITI could be useful to assess the PMS objectively. But more research should be needed.

• Proceedings of the KSME Conference
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• 2000.04b
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• pp.113-118
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• 2000

Experimental results describing the effects of blowing ratio on film cooling from two rows of holes with opposite orientation angles are presented. The inclination angle was fixed at $35^{\circ}$ and the orientation angles were set to be $45^{\circ}$ for downstream row. and $-45^{\circ}$ for upsream row. The studied blowing ratios were 0.5, 1.0 and 2.0. The boundary layer temperature distributions were measured using thermocouple at two downstream loundary layer temperature distributions were measured using thermocouple at two downstream locations. Detailed adiabatic film cooling effectiveness and heat transfer coefficient distributions were measured with TLC(Thermochromic Liquid Crystal). The adiabatic film cooling effectiveness and heat transfer coefficient distributions are discussed in connection with the injectant behaviors inferred from the boundary layer temperature distributions. Film cooling performance, represented by heat flux was calculated with the adiabatic film cooling effectiveness and heat transfer coefficient data.

• Journal of the Korean Institute of Electrical and Electronic Material Engineers
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• v.21 no.2
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• pp.144-150
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• 2008

Recently, there has been growing interest in low loss and low dielectric constant material for LTCC application, as the frequency range for electronic devices increases. This study was designed to evaluate the effect of cordierite filler for low dielectric constant LTCC material. From the previous experiments, two glass compositions of B-Si-Al-Zn-Ba-Ca-O and B-Si-Al-Sr-Ca-O system, were chosen. Each powder of two glass compositions was sintered respectively with commercial cordierite powder in temperature range from $800^{\circ}C\;to\;900^{\circ}C$. Crystalline cordierite and glass peaks were affected only with two factors of composition and sintering temperature among various factors. With the optimized condition of two cordierite/glass compositions, obtained dielectric constant was below 5.5 and quality factor was above 1,000. Closed pore of sintered body was controled by sintering temperature and sintering time. When cordierite/glass composite with ratio of 5.5:4.5 was sintered at $900^{\circ}C$, densification was sufficient with good dielectric characteristics of ${\epsilon}_r<5.1,\;Q{\ge}1,000$. Residual fine closed pores could be reduced with control of sintering temperature and time. 3 point bending strength and chemical durability were evaluated to obtain feasibility for substrate material.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.32 no.5
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• pp.359-366
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• 2008

A two-dimensional thermal model of proton exchange membrane fuel cell with large active area is developed to investigate the performance of fuel cell with large active area over various thermal management conditions. The core sub-models of the two-dimensional thermal model are one-dimensional agglomerate structure electrochemical reaction model, one-dimensional water transport model, and a two-dimensional heat transfer model. Prior to carrying out the simulation, this study is contributed to set up the operating temperature of the fuel cell with large active area which is a maximum temperature inside the fuel cell considering durability of membrane electrolyte. The simulation results show that the operating temperature of the fuel cell and temperature distribution inside the fuel cell can affect significantly the total net power at extreme conditions. Results also show that the parasitic losses of balance of plant component should be precisely controlled to produce the maximum system power with minimum parasitic loss of thermal management system.

• Journal of the Korean Wood Science and Technology
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• v.37 no.6
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• pp.517-523
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• 2009

Repeated impregnation and carbonization processes were performed to prepare high-density woodceramics using a sawdust board. The physical properties were investigated to confirm morphological and structural changes of one-time and two-time phenolic resin-treated and carbonized woodceramics. As comparing between one-time and two-time carbonized woodceramics, the weight and the density of the two-time carbonized woodceramics decreased with an increase of the carbonization temperature. When the carbonization temperature was $600^{\circ}C$, the weight increased by 21.7% and density increased by 20.6% from $0.68g/cm^3$ to $0.82g/cm^3$, respectively, as a maximum value.

• Korean Journal of Microbiology
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• v.14 no.1
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• pp.39-47
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• 1976

This experiment was designed to study the effects of temperature on the peroxidase isoenzymes of a mesophilic microorganism, Escherichia coli (grown within biokinetic zone). Optimum temperature for the growth of E. coli was $37{\circ}C.$ Three different temperatures, 20, 30 and $40{\circ}C,$ were selected. And the isoenzyme patterns of peroxidase of E. coli, growth respectively at each temperature, were analysed by disc electrophoresis. The sample of 20.deg.C showed 4 bands, that of 30.deg.C, 5 bands and that of 40.deg.C, 6 bands. Two dark bands (higher molecular weight, 56,000 and 54,000) and two light bands (lower molecular weight, 11,500 and 10,000) were constant at all samples. But two intermediate bands (M.W.44,000 and 34,000) were variable ; at $20{\circ}C,$ no banding pattern, one band 9M.W. 34,000) only at $30{\circ}C,$ and at $40{\circ}C$ two bands were appeared. And the shifts of growth temperatures between 30.deg.C and 40.deg.C showed the alteration of the isoenzyme patterns ; the isoenzyme patterns of the smaple of tmeperature shift from $30{\circ}C$ to $40{\circ}C$ were same as that of 40.deg.C and vice versa.

• Structural Engineering and Mechanics
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• v.90 no.2
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• pp.209-218
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• 2024

The present paper is focused on the study of the propagation of plane waves in thermoelastic media under a modified Green-Lindsay (MG-L) model having the influence of non-local and two temperature. The problem is formulated for the considered model in dimensionless form and is explained by using the reflection phenomenon. The plane wave solution of these equations indicates the existence of three waves namely Longitudinal waves (LD-Wave), Thermal waves (T-wave), and Shear waves (SV-wave) from a stress-free surface. The variation of amplitude ratios is computed analytically and depicted graphically against the angle of incidence to elaborate the impact of non-local, two temperature, and different theories of thermoelasticity. Some particular cases of interest are also deduced from the present investigation. The present study finds applications in a wide range of problems in engineering and sciences, control theory, vibration mechanics, and continuum mechanics.

• 한국정보디스플레이학회:학술대회논문집
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• 2009.10a
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• pp.1086-1087
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• 2009

A small temperature sensor is designed in a 0.35um CMOS process. Transistors operating in the intermediate inversion region are employed in the core of the sensor. This temperature sensor operates in $-50^{\circ}C{\sim}120^{\circ}C$ with ${\pm}2^{\circ}C$ of accuracy after two-point calibration. This temperature sensor can be placed in the active pixel area of a display panel to measure the temperature of the display panel for temperature compensation.

• International Journal of Air-Conditioning and Refrigeration
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• v.17 no.3
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• pp.107-113
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• 2009

The objective of this study is to investigate performance characteristics of a household refrigerator using a two-stage compression cycle. The performance of the two-stage compression cycle was measured by varying the compressor speed, condensing temperature, and evaporating temperature. The COP of the two-stage compression cycle was analyzed and then compared with that of the single-stage compression cycle. The optimum combination of compressor speeds for a low- and a high-stage was determined. The COP of the two-stage compression cycle using a PTC (parallel two-stage compression) method was 5.85% higher than that of a STC (serial two-stage compression) method at optimum operating conditions.