• Title/Summary/Keyword: tuber crop

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Analysis of Genes Activated by Salt and ER Stress in bZIP17 and bZIP28 Gene Transgenic Potato Plants

  • Kim, Kyung Hwa;Choi, Man Soo;Chun, Jae Buhm;Jin, Mi Na;Jeong, Nam Hee;Kim, Dool Yi
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2018.10a
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    • pp.179-179
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    • 2018
  • Potato (Solanum tubersosum L.) is susceptible to various environmental stresses such as salt, high temperature, and drought. Especially, potato tuber growth is greatly affected by drought that causes not only yield reduction but also loss of tuber quality. Since unpredictable global weather changes cause more severe and frequent water limiting conditions, improvement of potato drought tolerance can minimize such adverse effects under drought and can impact on sustainable potato production. Genetic engineering can be utilized to improve potato drought tolerance, but such approaches using endogenous potato genes have rarely been applied. We were obtained AtbZIP28 gene transgenic potato plants. It is identified transcript levels at various stress conditions, polyethylene glycol (PEG), NaCl, (ABA). Also, For identification to regulate ER stress response genes in AtbZIP28 gene transgenic potato plant, we screened seven potato genes from RNA-seq analysis under TM treatment. Five and two genes were up- and down-regulated by TM, respectively. Their expression patterns were re-examined at stress agents known to elicit TM, DTT, DMSO and salt stress.

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Influence of Polyethylene Film Mulch and Seedling Types on Growth and tuber Yield of Fall-Grown Potato (폴리에틸렌필름 멀칭 및 종묘종류가 가을감자의 생육과 수량에 미치는 영향)

  • 강봉균;강영길;강시용
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.48 no.3
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    • pp.147-151
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    • 2003
  • Normal seed tubers and plug seedlings raised from stem cutting and mini-tubers ($10\pm3$ g) were planted or transplanted on August 20,2001 in bare soil or under transparent and black polyethylene film mulches to evaluate the effects of mulch material and seedling type on the growth and tuber yield of fall-grown potato (Solanum tuberosum L. cv. Dejima) in Jeju region. Total tuber number per plant was 4.6 regardless of mulch treatment, and direct planting seed potatoes resulted in the greatest tubers (5.14)/plant and followed by transplanting seedlings raised from cutting and mini-tubers (4.15 and 5.15 tubers/plant). Transparent and black polyethylene film mulches increased total tuber yield by 16 and 8%, respectively, and average tuber weight by 14 and 12%, respectively, compared with no mulch (21.16 t/ha and 72.2 g/tuber). Transplanting plug seedlings raised from cutting resulted in the greatest tuber yield, followed by transplanting plug seedlings raised from mini-tubers and direct planting seed potatoes.

In Vitro Micropropagation of Chinese Yam (Dioscorea opposita Thunb.) through the Culture of Micro-tuber Sections and by Addition of Liquid Medium (영여자 절편체 배양 및 액체배지 첨가에 의한 둥근마의 기내 대량번식)

  • Kim, Young-Ho;Lim, Soon-Taek;Han, Bong-Hee
    • Korean Journal of Medicinal Crop Science
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    • v.20 no.3
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    • pp.190-194
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    • 2012
  • Shoot tips of chinese yam (Dioscorea opposita Thunb.) were cultured on MS medium containing 0.5 mg/L BA to produce micro-tubers in vitro. To stimulate the formation of shoots and micro-tubers, and produce large micro-tubers, the sections of micro-tubers were cultured on MS media with BA and IAA. The shoot multiplication, and the micro-tuber formation and growth were very effective on the media containing 2.0 mg/L BA and 0.5~1.0 mg/L IAA. Sucrose added to MS medium with 2.0 mg/L BA and 0.5 mg/L IAA to stimulate more micro-tuber growth. The medium added 50 g/L sucrose was very effective in the increase of plant fresh weight and micro-tuber growth. After 4 weeks' culture of micro-tuber sections on the medium with 2.0 mg/L BA, 0.5 mg/L IAA and 50 g/L sucrose, the liquid media were added into the same vessels. The micro-tuber growth was stimulated remarkably by the addition of liquid medium. The addition of 25 $m{\ell}$ liquid medium containing 10 g/L activated charcoal, 3x MS salts and 250 g/L sucrose was the most effective in micro-tuber growth.

Plant Regeneration and in vitro Tuber Enlargement from Callus in Pinellia ternata(Thunb.) Breit (반하(半夏) 캘러스로부터 식물체(植物體) 재생(再生)과 기내(器內) 괴경(塊莖) 생장(生長) 유도(誘導))

  • Kim, Tai-Soo;Park, Moon-Soo;Park, Ho-Ki;Kim, Sun;Jang, Yeong-Sun
    • Korean Journal of Medicinal Crop Science
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    • v.2 no.3
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    • pp.246-250
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    • 1994
  • This study carried out to induce plant regeneration and callus formation from leaflet in MS medium with 2.4-D, NAL and IAA for in vitro growth of tuber, Kinetin and BA were used for plant regeneration. $NH_4NO_3$ and $KNO_3$ as a nitrogen source and $(NH_4)_2SO_4$, as a sulfate source were tested for in vitro growth of tuber. The resutls ars as fellows : 1. For the callus formation from leaflet and differentiation potency of organ, 2.4-D was more effective than IAA in MS medium under $26^{\circ}C$ and light condition of 8 hours a day. 2. For the plant regeneration from callus, MS medium with $2.0\;mg/{\ell}$ BA was most effective under $26^{\circ}C$ and light condition of $16{\sim}24$ hours a day. 3. For the in vitro growth of tuber, $KNO_3$ by $3.0g/{\ell}$ in MS medium was effective. This condition enhanced the growth of tuber 2.5 times compared with that in MS medium with $2.0\;mg/{\ell}$ BA.

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Effects of Seed-piece Size and Seed-piece Number Per a Hydroponiclly Grown Mini-tuber on Growth and Yield of Potato (감자의 양액재배 소괴경의 절편크기 및 절편수에 따른 생육 및 수량)

  • 강봉균;강영길;문현기;송창길;김찬우;박정식
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.47 no.6
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    • pp.432-436
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    • 2002
  • This study was conducted in 2001 to determine the influence of seed-piece size (SPS) and number (SPN) per mini-tuber produced by hydroponics on growth and yield of potato (Solanum tuberosum L.). Treatments consisted of the combination of six SPS (3, 5, 7, 9, 11, 13g/seed-piece) and four SPN (whole tuber, and two, three, and four pieces/tuber). Percentage sprout emergence at transplanting was higher for seed pieces weighing 7 to 13 g (83.0 to 85.4%) and for three and four pieces per tuber (85.4 and 84.8%). As SPS were increased from 3 to 9 g/piece, total yields markedly increased from 2,052 to 2,924 kg/10a and seed tuber (31 to 250 g) yield from 1,589 to 2,428kg/10a with no further increase with SPS. As SPN was increased from one to three/ tuber, total yields increased from 1,918 to 2,982 kg/10a and seed tuber yields from 1,422 to 2,579 kg/10a with no further increase with SPN. Average tuber weight increased with SPS (46.3 to 71.5 g/tuber) and SPN (56.5 to 64.9 g/tuber).

Development of Recirculating Wick Hydroponic Techniques for Safe Seed Tuber Multiplication of Potatoe (심지 양액재배법에 의한 상위급 씨감자의 안정적 대량생산)

  • Kang Bong Kyoon;Kim Chan Woo
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.49 no.6
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    • pp.447-451
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    • 2004
  • This study was conducted to establish the proper techniques of the recirculating wick hydroponics for safe seed tuber proliferation of potatoes (Solanum tuberosum L. CV. Dejima). To achieve these, several intact tubers (5, 10 and 20 g) and cut seed-pieces (two or four) were treated in wick hydroponic system beds. A polystyrene box (31cm in width, 20cm in height, 51cm in length, and $0.031m^3$ in volume) was placed on a styrofoam hox. Eight wicks $(width\;1.5cm\;{\times}\;length\;40cm) $ were put into each polystyrene hox and the boxes were filled with perlite + peatmoss (1 : 2, v/v) medium. Top fresh weight per plant increased with increasing the tuber size from 10 to 30g/tuber. As the tuber size increases from 10 to 30 g/tuher in case of uncut tuber, the marketable tuber (>5g) production per plant increased from 83.8 to 141.8 g/plant and the marketable tuber (>5g) production per plant of cut tuber was slightly higher than that of uncut treatment. Total tuber yield ranged from 4.16 to $6.56kg/m^2$ and the percentage of seed tubers was greater than $97\%$ for all treatments. These results indicate that seed tuber should be cut to produce large tuber $(\geq10g)$ in the recirculating wick hydroponic system.

Description of the Diadegma fenestrale (Hymenoptera: Ichneumonidae: Campopleginae) Attacking the Potato Tuber Moth, Phthorimaea operculella (Lep.: Gelechiidae) New to Korea

  • Choi, Jin-Kyung;Kim, Ju Il;Kwon, Min;Lee, Jong-Wook
    • Animal Systematics, Evolution and Diversity
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    • v.29 no.1
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    • pp.70-73
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    • 2013
  • Diadegma fenestrale is known as a parasitoid of the potato tuber moth, Phthorimaea operculella. The potato tuber moth, Phthorimaea operculella (Zeller) is one of the most destructive pest of potatoes. Also, we found this species attacking the diamondback moth, Plutella xylostella (Lepidoptera: Plutellidae). Ratio of parasitism is 20-30% and cocoon of lepidopteran was parasitic ichneumonid species after 3 days. This species and the genus Diadegma are recorded for the first time from Korea. In this paper, description of the parasitoid and photographs of the diagnostic characteristics are provided.

Introduction of Yam Bean (Pachyrhizus spp.) in Korea

  • Kim, Sang-Kuk;Choi, Hong-Jib;Won, Jae-Hee;Park, Jun-Hong;Lee, In-Jung;Park, Shin-Young
    • Korean Journal of Plant Resources
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    • v.22 no.6
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    • pp.546-551
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    • 2009
  • We examined the growth characteristics, fresh tuber yield, rotenone content of two yam beans (Pachyrhizus erosus and P. ahipa) introduced from Mexico. P. erosus species showed better adaptable ability than P. ahipa species in seed productivity and tuber yield. Rotenone content in the P. erosus species extracted with chloroform was 3.6 folds much more extracted than ethanol extraction. The order of rotenone content found in crude extract obtained by different solvent extraction from the highest to the lowest was mature seed (484.7 ${\mu}g$) and leaves (17.2 ${\mu}g$) of the P. erosus species, respectively.

Effects of Altitude and Planting Time on Tuber Bulking of Potato (감자 고랭지 재배 시 표고 및 파종시기에 따른 괴경의 비대반응)

  • Kim, Chung-Guk;Ok, Hyun-Chung;Jeong, Jin-Chol;Hur, On-Sook;Seo, Jong-Ho;Jeong, Kwang-Ho;Kim, Si-Ju
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.57 no.4
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    • pp.418-423
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    • 2012
  • Field experiments were carried out to improve the cultural practice of potato by analysing its tuberization patterns. Tuberization patterns affected by different altitudes was analyzed at two potato cultivation regions, Jinbu (600 m) and Daegwallyung (800 m) using two potato cultivars, 'Superior' and 'Atlantic'. To analyse tuberization patterns affected by different planting time, seed potatoes were planted at every 10 days from April 19 to May 19 in Daegwallyung. Total dry weight was greater in plant grown at the altitude of 800 m than 600 m during the entire growth period and the highest increase was observed at the early growth period, July 6, comparing to other growth period. The total dry weight was the greatest at 110~112 days after planting (DAP) at the altitude of 800 m and 108~111 DAP at 600 m. There was no significant differences between altitudes and between cultivars. Tuber dry weight per plant at the altitude of 600 m was lower than 800 m on July 6 (58 DAP), but it increased rapidly from July 21 (73 DAP). At both altitudes, the increase of tuber dry weight per plant from July 6 to August 8 was higher than the other growth period. The time of growth period at which tuber dry weight per plant was the highest was similar at both altitudes that was 118~125 DAP at the altitude 800 m and 118~124 DAP at the altitude 600 m. Dry weight per tuber at the altitude 800m was higher than 600 m due to the number of tubers per plant. A higher increase of crop growth rate (CGR) was shown at the altitude 600 m on July 6 (58 DAP), comparing to at 800 m. The highest tuber dry weight per plant of each cultivar was shown when the planting time was April 29 for 'Superior' and was April 19 for 'Atlantic'. Both the tuber dry weight of plant and the total dry weight were lower at a later planting time. Dry weight per tuber increased quickly during the period between June 30 to August 8. Tuberization period was shortened as the planting time was delayed.

Dormancy-related Change in Endogenous ABA, Batatasin, and Sugar in Stored Tuber and Bulbil of Chinese Yam

  • Lee, In-Jung;Kim, Sang-Kuk;Lee, Sang-Chul;Park, Tae-Shik;Kwon, Soon-Tae
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.47 no.4
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    • pp.297-300
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    • 2002
  • Endogenous ABA and batatasins were first quantified in the stored tubers and bulbils of the Chinese yam, Dioscorea opposita Thunb. cv. 'Tsukune' by GC-MS with comparison of internal standard, and sugar content was also analyzed by HPLC with comparison of external standard. Endogenous ABA content in stored tubers and bulbils was rapidly decreased as storage period prolonged. ABA content of the bulbils was always higher than that of tuber during storage periods. All batatasins of stored tubers and bulbils at 30 days after storage decreased steadily compared to 120 days after storage. On the contrary, batatasin-Ⅲ of the stored tuber and bulbils was only decreased at 60 days after storage. In Dioscorea opposita Thunb. cv. 'Tsukune' like D. japonica, D. alata, and D. bulbifera, may be controlled by endogenous batatasins and ABA, In these compounds, batatasins rather than ABA might be closely related to dormancy-inducing compound during dormancy of the Chinese yam, Dioscorea opposita Thunb. cv. Tsukune.