Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
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In Vitro Micropropagation of Chinese Yam (Dioscorea opposita Thunb.) through the Culture of Micro-tuber Sections and by Addition of Liquid Medium

영여자 절편체 배양 및 액체배지 첨가에 의한 둥근마의 기내 대량번식

  • Kim, Young-Ho (Chungbuk Agricultural Research and Expression Services) ;
  • Lim, Soon-Taek (The Foundation of Agriculture Technology Commercialization and Transfer) ;
  • Han, Bong-Hee (The Foundation of Agriculture Technology Commercialization and Transfer)
  • Received : 2012.04.12
  • Accepted : 2012.06.14
  • Published : 2012.06.30
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Abstract

Shoot tips of chinese yam (Dioscorea opposita Thunb.) were cultured on MS medium containing 0.5 mg/L BA to produce micro-tubers in vitro. To stimulate the formation of shoots and micro-tubers, and produce large micro-tubers, the sections of micro-tubers were cultured on MS media with BA and IAA. The shoot multiplication, and the micro-tuber formation and growth were very effective on the media containing 2.0 mg/L BA and 0.5~1.0 mg/L IAA. Sucrose added to MS medium with 2.0 mg/L BA and 0.5 mg/L IAA to stimulate more micro-tuber growth. The medium added 50 g/L sucrose was very effective in the increase of plant fresh weight and micro-tuber growth. After 4 weeks' culture of micro-tuber sections on the medium with 2.0 mg/L BA, 0.5 mg/L IAA and 50 g/L sucrose, the liquid media were added into the same vessels. The micro-tuber growth was stimulated remarkably by the addition of liquid medium. The addition of 25 $m{\ell}$ liquid medium containing 10 g/L activated charcoal, 3x MS salts and 250 g/L sucrose was the most effective in micro-tuber growth.

Keywords

References

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