• Journal of Advanced Research in Ocean Engineering
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• v.1 no.3
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• pp.157-167
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• 2015

In order to provide simple and accurate wave theory in design of offshore structure, an analytical approximation is introduced in this paper. The solution is limited to flat bottom having a constant water depth. Water is considered as inviscid, incompressible and irrotational. The solution satisfies the continuity equation, bottom boundary condition and non-linear kinematic free surface boundary condition exactly. Error for dynamic condition is quite small. The solution is suitable in description of breaking waves. The solution is presented with closed form and dispersion relation is also presented with closed form. In the last century, there have been two main approaches to the nonlinear problems. One of these is perturbation method. Stokes wave and Cnoidal wave are based on the method. The other is numerical method. Dean's stream function theory is based on the method. In this paper, power series method was considered. The power series method can be applied to certain nonlinear differential equations (initial value problems). The series coefficients are specified by a nonlinear recurrence inherited from the differential equation. Because the non-linear wave problem is a boundary value problem, the power series method cannot be applied to the problem in general. But finite number of coefficients is necessary to describe the wave profile, truncated power series is enough. Therefore the power series method can be applied to the problem. In this case, the series coefficients are specified by a set of equations instead of recurrence. By using the set of equations, the nonlinear wave problem has been solved in this paper.

• Molecules and Cells
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• v.37 no.7
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• pp.526-531
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• 2014

Human PARP family consists of 17 members of which PARP-1 is a prominent member and plays a key role in DNA repair pathways. It has an N-terminal DNA-binding domain (DBD) encompassing the nuclear localisation signal (NLS), central automodification domain and C-terminal catalytic domain. PARP-1 accounts for majority of poly-(ADP-ribose) polymer synthesis that upon binding to numerous proteins including PARP itself modulates their activity. Reduced PARP-1 activity in ageing human samples and its deficiency leading to telomere shortening has been reported. Hence for cell survival, maintenance of genomic integrity and longevity presence of intact PARP-1 in the nucleus is paramount. Although localisation of full-length and truncated PARP-1 in PARP-1 proficient cells is well documented, subcellular distribution of PARP-1 fragments in the absence of endogenous PARP-1 is not known. Here we report the differential localisation of PARP-1 Nterminal fragment encompassing NLS in PARP-$1^{+/+}$ and PARP-$1^{-/-}$ mouse embryo fibroblasts by live imaging of cells transiently expressing EGFP tagged fragment. In PARP-$1^{+/+}$ cells the fragment localises to the nuclei presenting a granular pattern. Furthermore, it is densely packaged in the midsections of the nucleus. In contrast, the fragment localises exclusively to the cytoplasm in PARP-$1^{-/-}$ cells. Flourescence intensity analysis further confirmed this observation indicating that the N-terminal fragment requires endogenous PARP-1 for its nuclear transport. Our study illustrates the trafficking role of PARP-1 independently of its enzymatic activity and highlights the possibility that full-length PARP-1 may play a key role in the nuclear transport of its siblings and other molecules.

• Steel and Composite Structures
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• v.48 no.4
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• pp.443-459
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• 2023

This paper investigates the stability of a bi-directional functionally graded (BD-FG) cylindrical beam made of imperfect concrete, taking into account size-dependency and the effect of geometry on its stability behavior. Both buckling and dynamic behavior are analyzed using the modified coupled stress theory and the classical beam theory. The BD-FG structure is created by using porosity-dependent FG concrete, with changing porosity voids and material distributions along the pipe radius, as well as uniform and nonuniform radius functions that vary along the beam length. Energy principles are used to generate partial differential equations (PDE) for stability analysis, which are then solved numerically. This study sheds light on the complex behavior of BD-FG structures, and the results can be useful for the design of stable cylindrical microstructures.

• Advances in concrete construction
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• v.15 no.4
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• pp.215-228
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• 2023

The vibrational behavior of nanoelements is critical in determining how a nanostructure behaves. However, combining vibrational analysis with stability analysis allows for a more comprehensive knowledge of a structure's behavior. As a result, the goal of this research is to characterize the behavior of nonlocal nanocyndrical beams with uniform and nonuniform cross sections. The nonuniformity of the beams is determined by three distinct section functions, namely linear, convex, and exponential functions, with the length and mass of the beams being identical. For completely clamped, fully pinned, and cantilever boundary conditions, Eringen's nonlocal theory is combined with the Timoshenko beam model. The extended differential quadrature technique was used to solve the governing equations in this research. In contrast to the other boundary conditions, the findings of this research reveal that the nonlocal impact has the opposite effect on the frequency of the uniform cantilever nanobeam. Furthermore, since the mass of the materials employed in these nanobeams is designed to remain the same, the findings may be utilized to help improve the frequency and buckling stress of a resonator without requiring additional material, which is a cost-effective benefit.

• Journal of the Computational Structural Engineering Institute of Korea
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• v.28 no.2
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• pp.123-130
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• 2015

This paper presents a new formulation for transient simulations of microwave propagation in heterogeneous unbounded domains. In particular, perfectly-matched-layers(PMLs) are introduced to allow for wave absorption at artificial boundaries used to truncate the infinite extent of the physical domains. The development of the electromagnetic PML targets the application to engineering mechanics problems such as structural health monitoring and inverse medium problems. To formulate the PML for plane electromagnetic waves, a complex coordinate transformation is introduced to Maxwell's equations in the frequency-domain. Then the PML-endowed partial differential equations(PDEs) for transient electromagnetic waves are recovered by the application of the inverse Fourier transform to the frequency-domain equations. A mixed finite element method is employed to solve the time-domain PDEs for electric and magnetic fields in the PML-truncated domain. Numerical results are presented for plane microwaves propagating through concrete structures, and the accuracy of solutions is investigated by a series of error analyses.

• Journal of Life Science
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• v.23 no.3
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• pp.347-354
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• 2013

Innate immunity is the ability to differentiate infectious agents from self. The innate immune system is comprised of a complicated network of recognition and effector molecules that act together to protect the host in the early stage of an infectious challenge. Mannose-binding lectin (MBL or mannose-binding protein, MBP) belongs to the family of $Ca^{2+}$-dependent lectins (C-type lectin with a collagen-like domain), which are considered an important component of innate immunity. While it is associated with increased risk and severity of infections and autoimmunity, the most frequent immuno-deficiency syndrome was reported to be low MBL level in blood. Deficiency of human MBL is caused by mutations in the coding region of the MBL gene. Rat homologue gene of human MBL gene was used to study functions of wild type and mutant MBL proteins. Although extensive studies have yielded the structural information of MBL, the functions of MBL, especially mutant MBL, still require investigation. We previously reported the cloning of rat wild-type MBL gene and the production of a truncated form of MBL protein and its antibody. Here, we present the cloning of mutant MBL cDNA in collagen-like domain (R40C, G42D, and G45E) using site-directed mutagenesis and differential behaviors of wild type and mutant MBL in cells. The major difference between wild type and mutant MBL was that while wild type MBL was secreted, mutant MBL was inhibited for secretion, retained in endoplasmic reticulum, and still functioned as a lectin.

• Korean Journal of Plant Tissue Culture
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• v.24 no.5
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• pp.305-311
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• 1997

Bacillus thuringiensis, a gram-positive soil bacterium, is characterized by its ability to produce crystalline inclusions during sporulation. The crystal proteins exhibit a highly specific insecticidal activity. An insecticidal crystal protein (ICP), Cry II A, is specifically toxic to both lepidopteran and dipteran insects. In this study, tobacco plants transformed by the cry II A gene have been generated. The Cry II A crystal protein was purified from E. coli JM103 harboring cry II A gene by differential solubility. The activated Cry II A was prepared by tryptic digestion. The purified protoxin (70 kDa) and the activated toxin (50 kDa) were analyzed by SDS-PAGE. To generate the transgenic tobacco having cry II A gene, the cry II A gene was subcloned to a plant expression vector, pSRL2, having two CaMV 35S promoters. The recombinant plasmid was transformed into tobacco (N. tabacum var. Petit Havana SR1) by Agrobacterium-mediated leaf disc transformation. Through the regeneration, six putative transgenic tobacco plants were obtained and three transformants were confirmed by Southern blot analysis. It has been found that one plant had single copy of cry II A gene, another had two copies of the gene, and the third had a truncated gene. After the immunochemical confirmation of cry II A expression in plants, the transgenic tobacco plants will be used to study the genetics of future generation with the insecticidal crystal protein gene cry II A.