• The Korean Journal of Malacology
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• v.26 no.4
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• pp.297-302
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• 2010

Water temperature, salinity and density on egg development of the sunray surf clam, Mactra chinensis were investigated for artificial seedling production. The required time from fertilization to D-shaped larvae were 33.8 hours in $18^{\circ}C$, 20.6 hours in $23^{\circ}C$, 18.2 hours in $28^{\circ}C$ and 15.0 hours in $33^{\circ}C$. The development duration was reduced with increasing temperature. The relationships between temperature and the required time from egg to each developmental stage were described as follows: 2-cell, 1/h = 0.1051WT - 1.4782; 8-cell, 1/h = 0.037WT - 0.3686; gastrula, 1/h = 0.008WT - 0.0521; trochophore, 1/h = 0.0041WT - 0.0235; D-shaped larva, 1/h = 0.0024WT - 0.0102. Biological minimum temperature for the egg development was estimated to be $8.0^{\circ}C$ in average. The possible range of temperature for the development of D-shaped larvae was $18-28^{\circ}C$ and optimum of water temperature for the development of egg was $23^{\circ}C$. The possible range of salinity for the development of D-shaped larvae was 20-35 psu and optimum of salinity for the development of egg was 30-35 psu over 25 psu at least. The density of fertilized egg was below 40 per 1 ml in rearing seawater for elevating the development rate from fertilized egg to D-shaped larva.

• The Korean Journal of Malacology
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• v.21 no.2 s.34
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• pp.107-111
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• 2005

In the present study, the fecundation and fertilization behaviors of the trumpet shell, Charonia fampas sauliae were observed from November 2003 to March 2004. Female has multiple fecundation and multiple fertilization, and the fertilization continued for two days. The fertilized female stayed with the egg mass to protect the eggs, and the fecundation was made during the fertilization. The highest survival rate of egg capsule was 60% at $15^{\circ}C$. Charonia lampas sauliae fed with Chaetoceros calcitrans, Isochysis galbana, and Pavlova lutheri. Growth and survival rates of C. lampas sauliae in the conditions of various water temperature were observed. The highest survival rate was 23% at $15^{\circ}C$ reared for 60 days. The growth was presented in the $15^{\circ}C$ group, with shell length from $408{\pm}21.52{\mu}m\;to\;625{\pm}19.76{\mu}m$ during the experiment.

• Journal of Aquaculture
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• v.17 no.2
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• pp.97-102
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• 2004

We examined the effects of stimulants including sunlight and UV-irradiation on the spawning induction and early development of the noble scallop, Chlamys nobilis. The sunlight stimulation resulted in nuch faster spawning induction (100% success within 40 minutes) compared to UV-irradiation (100% success within 70 minutes). Early development of the scallop larva took place between 15$^{\circ}C$ to 3$0^{\circ}C$. The time to reach the early D-shaped stage was 63.5, 31.5, 18.5 and 17.0 hours at 15, 20, 25 and 3$0^{\circ}C$, respectively. The correlations between the water temperature-(WT) regimes and the time (t) required for each developmental stage are as follows. 2 cell stage: 1/t=0.0606WT-0.6194 ($r^2$=0.9791) 8 cell stage: 1/t=0.0304WT-0.3453 ($r^2$=0.9941) Morula: 1/t=0.0100WT-0.1049 ($r^2$=0.9663) Trochophore: 1/t=0.0058WT-0.0618 ($r^2$=0.9848) D-shaped larva: 1/t=0.0030WT-0.0282 ($r^2$=0.9731) These correlations indicated that the biological minimum temperature of the species is around 10.44$^{\circ}C$. The highest survival rate up to D-shaped larva at different water temperature was observed at $25^{\circ}C$.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.11 no.2
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• pp.115-121
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• 1978

Early developmental stages of Mactra chinensis were bioassayied to determin the water quality of the costal waters of Busan. The water samples were obtained at 12 stations from July 12 to July 17, 1977. Water quality evaluation was made in terms of the rates of normal development to abnormal development in three indicatory states, i. e. fertilization membrane formation, morula and trochophore larvae. The pollution degree of the waters brought from five swimming beaches was found to be highest at Haewundae followed by Songdo, Kwang-anri, Songjeong and Dadaepo with decreasing order. The highest value of the water at Haewundae was mainly due to the accidental oil spill from an oil tanker which happened on July 7, 1977. At Dongsamdong the rate of total abnormal development was $47.17\%$. This result is apparently attributable to fish byproducts discarded from a number of restaurants. At Chungmudong where a large fish-market is located, the rate of total abnormal development was $61.14\%$. At Suyeng Bay and Yongho Bay the rates of total abnormal development were $73.82\%$ and $72.90\%$ respectively. At these bays the drainage presumably contains a large amount of chemical pollutants from the industrial areas. This result shows that no organisms can normally breed in these regions.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.6 no.1_2
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• pp.76-80
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• 1973

Cyclina sinensis is an edible bivalve inhabiting wide tidal flats which are exposed to the air at ebb tide along the western coast of Korea. Over the period of June to September 1971, some specimens from a tidal flat near Inchon were submerged in sea water with various concentrations of ammonium hydroxide added and careful observations were made on their fertilization, early development, and metamorphosis of the larvae. The highest rate of fertilization was demonstrated by individuals treated with 1/1000 normal solution of ammonium hydroxide and their fertilized eggs followed normal development, i.e., two cell stage 1.5 hours after fertilization, blastular stage after 4 hours, and trochophore stage after 6 hours. Within 24 hrs after fertilization C. sinensis larvae have acquired the form of early straight-hinge veliger with the mean prodissoconch I with the length of $110\mu$. It takes seven days to get the umbo stage with the mean shell length of $190\mu$ and twenty days to get the morphosing stage with the mean shell length of $260\mu$. The larvae were cultured to the metamorphosing stage with the shell length of $270\mu$ in the laboratory condition.

• Journal of Aquaculture
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• v.16 no.1
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• pp.24-30
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• 2003

Studies of the seed production of Urechis unicinctus were conducted under the laboratory conditions to obtain some information for the U.unicinitus culture. The experiment included developmental studies of the egg development, larval culture, sediment preference and growth of young U.unicinctus. The experiment were conducted from March to August, 2000. The adults of U.unicinctus collected in Namhae-do, Korea. The developments of the fertilized eggs were observed under a light-microscope at intervals of one hour after containing with density of one individual per 1 $m\ell$. The larvae were fed with Phaeodactylum tricornutum cultured at the laboratory. The concentration of the phytoplankton for the feed was 30,000 cells per individual larva. With progress of development, the food concentration was gradually increased, up to 10,000 cells per individual for the young U.unicinctus. Trochophore larvae appeared on the 68 hours after hatching. On the 32 days after hatching, over 50% of fertilized eggs developed into young U.unicinctus. In order to investigate the effect of sediment on the growth and burrowing of U.unicinctus, the young worms were reared in tanks with different grain sizes. The highest value of sediment preference and survival rate of U.unicinctus was shown in the mixture sediment group with below 0.10 mm, 1.01∼12.00 mm, over 3.01 mm and shell. The lowest value in both sediment preference and survival rate of U.unicinctus was observed in 1.0l∼2.00 mm grain size.

• The Korean Journal of Malacology
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• v.28 no.2
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• pp.145-156
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• 2012

Larval development of the Manila clam Ruditapes philippinarum reared in an indoor tank system was examined in this study using light microscope and scanning electron microscope. To induce spawning and subsequent larval development, clams were collected from the intertidal zone at Gim-nyeong harbor in Jeju Island in August 2011. After 2 days of rearing in the tank, all Manila clams spawned in the midnight. Non-feeding trochophore larvae appeared 7hrsafter fertilization and the first D-shape larvae could be observed at 19 hrs. Twenty one days after fertilization the pediveliger larvae crawling on the bottom of the tank with well-developed foot were observed. Histology indicated that all the clams used in this study were in the ripe stage prior to spawning and the gonad-somatic index (GSI), a ratio of the egg mass to the tissue weight, of the ripe female measured by ELISA was 28.6%. The GSI of female clam declined to 17.3% after the massive spawning in the tank, suggesting that Manila clam discharged 40% of the total eggs during the first spawning event. In conclusion, spawning and subsequent larval development of Manila clam was successfully carried out in this study using an indoor tank system, and the information obtained in the present study could be useful in future Manila clam hatchery development.

• The Korean Journal of Malacology
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• v.29 no.4
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• pp.263-272
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• 2013

The development of swift's scallop Chlamys swiftii, reared in the laboratory, has been examined through the investigation of morphological characteristics in fertilized egg, larvae and juvenile. Eggs were fertilized with a dilute sperm solution to improve the survival of fertilized eggs. Developing larvae were maintained at a temperature of $16{\pm}0.5^{\circ}C$ and salinity concentration of 33 ppt. We have investigated the fertilization rates and egg number spawned at several stimulating conditions such as sunlight exposure, air dry, seawater temperature rise ($5^{\circ}C$) and seawater temperature rise ($5^{\circ}C$) after exposure of air dry for spawning induction of swift's scallop Chamys swiftii. Stimulation treated with sunlight exposure and seawater temperature rise ($5^{\circ}C$) have shown the spawning number of 700,000-900,000 and 700,000-800,000 per individual, respectively while stimulation treated with seawater temperature rise ($5^{\circ}C$) after exposure of air dry have shown the high spawning number of 1,000,000-1,500,000 per individual. Survival rate of D-shaped larvae of swift's scallop put into the different seawater temperatures of $8^{\circ}C$, $12^{\circ}C$, $16^{\circ}C$, $20^{\circ}C$ and $24^{\circ}C$ has been 4.1%, 11.6%, 32.7%, 18.6% and 3.2%, respectively. Fertilized eggs with the diameter of $72{\mu}m$ developed into trochophore larvae of $103{\pm}3.8{\mu}m$ shortly after 35 hours and to D larvae of $129{\pm}10.4{\mu}m$ shell length within 72 hours. It took 336 hours to become initial Umbo-stage larva of $145{\pm}16.8{\mu}m$ shell length. Post larvae, which have been $197{\pm}13.6{\mu}m$ shell length, spontaneously have settled in the attachment substances. It have required 528 hours from fertilized eggs to early attached juvenile to become initial juvenile size of $245{\pm}15.8{\mu}m$ shell length.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.8 no.3
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• pp.185-195
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• 1975

The comparative studies Were conducted with respect to the artificial spawning early embryonic development, metamorphosis and growth of two species Meretrix lusoria and Cyclina sinensis collected from Inchon, Anmyon island and Buan areas from 1969 to 1974. The highest rate of artificial spawning of M. lusoria, which treated with a dilute ammoniun hydroxide(4/100-5/100N)-seawater solutions, was $25.0-33.3\%$, whereas in C. sinensis the rate of spawning was lower than that of M, lusoria under the similar experimental conditions$(12.5-19.0\%)$. However, the rate of artificial spawning of C. sinensis increased $40\%$ by repeated thermal stimulation. The rate of artificial fertilization of M. lusoria and C. sinensis showed highest value from those individuals which were treated with 1/1000N $NH_4OH$ solution. Their fertilized eggs, then, showed a normal development in the 1/1000N $NH_4OH$ solution. In the early embryonic development of M. lusoria and C. sinensis, the appearance of each of polar body, trochophore and D-shaped veliger were observed around 50min. 5-6 hours, and 23 hours after artificial fertilization respectively. The larval shell lengths of M. lusoria reached to $109,5{\pm}0.7\mu,\;144.6{\pm}1.3\mu$ and $208.0{\pm}0.0\mu$ around, 1, 11 and 20 days, after fertilization respectively. The larval shell lengths of C. sinensis reached to $110.5{\pm}0.6\mu,\;147.8{\pm}1.7\mu,\;and\;235.0{\pm}0.0\mu$ around 1, 10 ana 20 days, after fertilization respectively. The correlations of relative growth rate between the shell length(L) and sell height(H) found by the following simple formula from D-shaped veliger to metamorphosing stage. H=0.77L+6.82 for M. lusoria H=0.75L+8.50 for C. sinensis.

• The Korean Journal of Malacology
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• v.17 no.1
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• pp.45-55
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• 2001

Gametogenes, reproductive cycle, first sexual maturity(biological minimum size), sex ratio and larval development of the marsh clam Corbicula japonica were investigated monthly by histological observations. Samples were collected in brackish water of Gokgang stream, Kyungsangbuk-Do, Korea, from August 1997 to July 1998. Sexuality of Corbicula japonica is dioecious and the species are an oviparous clam. The gonads are irregularly arranged from the sub-region of mid-intestinal gland in visceral cavity to reticular connective tissue of foot. The ovary is composed of a number of ovarian sac which are branched arborescent. Oogonia actively proliferate along the germinal epithelium of ovarian sac, in which young oocytes are growing. The testis is composed of a number of testicular tubules, and the epithelium of the tubule has function of germinal epithelium, along which spermatogonia actively proliferate. A great number of undifferentiated mesenchymal tissue and eosinophilic granular cells are abundantly distributed between developing oocytes and spermatocytes in the early developmental stages. With the further development of the ovary and testis these tissue and cells gradually disappear. Then the undifferentiated mesenchymal tissue and eosinophilic granular cells are considered to be related to the growing of the oocytes and spermatocytes. The spawning period is from July to September, and the main spawning occur between July and August when seawater temperatures reach above 22$^{\circ}C$. The reproductive cycle of this species can be divided into five successive stages; early active (February to April), late active (May to July), ripe (June to September), partially spawned (July to September), degenerative (September to October) and resting stage (October to February). Percentages of first sexual maturity of female and male clams ranging in length from 10 mm to 12 mm are over 50% and 100% for clams over 16.0 mm in shell length. Fertilized eggs or Corbicula japonica were 80-90 ${\mu}{\textrm}{m}$ in diameter. In the early embryonic development of C. japonica, the appearance of polar body, trochophore and D-shaped veliger were observed around 40 min., 27 hours and 4 days after spawning, respectively, at a water temperature of 26.5-28.$0^{\circ}C$. The size of larvae of early umbo stage was about 185-210 ${\mu}{\textrm}{m}$ in shell length, 160-180 ${\mu}{\textrm}{m}$ in shell height around 7 days after fertilization. The correlation of relative growth between the culture day (D) and shell length (SL) was expressed by the following simple formula from D-shaped veliger to metamorphosing stage; SL = 13.300D + 209.36($r^2$= 0.9078).