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Intrageneric Relationships of Trichoderma Based on Internal Transcribed Spacers and 5.8S rDNA Nucleotide Sequences

  • Kim, Gi-Young;Lee, Goang-Jae;Ha, Myung-Gyu;Lee, Tae-Ho;Lee, Jae-Dong
    • Mycobiology
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    • v.28 no.1
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    • pp.11-16
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    • 2000
  • The nucleotide sequences of the internal transcribed spacer (ITS) regions of the ribosomal DNA including the 5.8S ribosomal RNA gene (rDNA) have been determined for 11 species in order to analyze their intrageneric relationships. The total length of these sequences ranged from 530 nucleotides for Trichoderma reesei KCTC 1286 to 553 nucleotide for Trichoderma koningii IAM 12534. Generally speaking, the length of ITS1 region was about 30 nucleotides longer than that of the ITS2 region. Also, the sequences of 5.8S rDNA were more conserved in length and variation than those of ITS regions. Although the variable ITS sequences were often ambiguously aligned, the conserved sites were also found. Thus, a neighbor-joining tree was constructed using the full sequence data of the ITS regions and the 5.8S rDNA. The Trichoderma genus used to be grouped on the basis of the morphological features and especially the shape of phialides needs to be reexamined. The phylogenetic tree displayed the presence of monophylogeny in the species of Trichoderma. Therefore, it was difficult to distinguish the intrageneric relationships in the Trichoderma genus.

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An AFLP-based Linkage Map of Japanese Red Pine (Pinus densiflora) Using Haploid DNA Samples of Megagametophytes from a Single Maternal Tree

  • Kim, Yong-Yul;Choi, Hyung-Soon;Kang, Bum-Yong
    • Molecules and Cells
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    • v.20 no.2
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    • pp.201-209
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    • 2005
  • We have constructed an AFLP-based linkage map of Japanese red pine (Pinus densiflora Siebold et Zucc.) using haploid DNA samples of 96 megagametophytes from a single maternal tree, selection clone Kyungbuk 4. Twenty-eight primer pairs generated a total of 5,780 AFLP fragments. Five hundreds and thirteen fragments were verified as genetic markers with two alleles by their Mendelian segregation. At the linkage criteria LOD 4.0 and maximum recombination fraction 0.25(${\theta}$), a total of 152 markers constituted 25 framework maps for 19 major linkage groups. The maps spanned a total length of 2,341 cM with an average framework marker spacing of 18.4 cM. The estimated genome size was 2,662 cM. With an assumption of equal marker density, 82.2% of the estimated genome would be within 10 cM of one of the 230 linked markers, and 68.1% would be within 10 cM of one of the 152 framework markers. We evaluated map completeness in terms of LOD value, marker density, genome length, and map coverage. The resulting map will provide crucial information for future genomic studies of the Japanese red pine, in particular for QTL mapping of economically important breeding target traits.

Length of stay in PACU among surgical patients using data mining technique (데이터 마이닝을 활용한 외과수술환자의 회복실 체류시간 분석)

  • Yoo, Je-Bog;Jang, Hee Jung
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.14 no.7
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    • pp.3400-3411
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    • 2013
  • The data mining is a new approach to extract useful information through effective analysis of huge data in numerous fields. This study was analyzed by decision making tree model using Clementine C&RT(Classification & Regression Tree, CART) as data mining technique. We utilized this data mining technique to analyze medical record of 1,500 people. Whole data were assorted by length of stay in PACU and divided into 3 groups. The result extracted by C5.0 decision tree method showed that important related factors for lengh of stay in PACU are type of operation, preoperative EKG abnormality, anesthetics, operative duration, age.

A Space Efficient Indexing Technique for DNA Sequences (공간 효율적인 DNA 시퀀스 인덱싱 방안)

  • Song, Hye-Ju;Park, Young-Ho;Loh, Woong-Kee
    • Journal of KIISE:Databases
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    • v.36 no.6
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    • pp.455-465
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    • 2009
  • Suffix trees are widely used in similar sequence matching for DNA. They have several problems such as time consuming, large space usages of disks and memories and data skew, since DNA sequences are very large and do not fit in the main memory. Thus, in the paper, we present a space efficient indexing method called SENoM, allowing us to build trees without merging phases for the partitioned sub trees. The proposed method is constructed in two phases. In the first phase, we partition the suffixes of the input string based on a common variable-length prefix till the number of suffixes is smaller than a threshold. In the second phase, we construct a sub tree based on the disk using the suffix sets, and then write it to the disk. The proposed method, SENoM eliminates complex merging phases. We show experimentally that proposed method is effective as bellows. SENoM reduces the disk usage less than 35% and reduces the memory usage less than 20% compared with TRELLIS algorithm. SENoM is available to query efficiently using the prefix tree even when the length of query sequence is large.

Optimal Location of Mail Distribution Center using Steiner Tree (Steiner Tree 이론을 이용한 우편물 교환센터인 최적 워치선정)

  • Yang, Seong-Deog;Lyu, Woong-Gyu;Lee, Sang-Joong
    • Journal of the Korean Institute of Illuminating and Electrical Installation Engineers
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    • v.22 no.9
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    • pp.82-87
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    • 2008
  • Faster, safer and cheaper mailing of the postal matter is essential for surviving in the competitive market of home-delivery service. In the past, the domestic delivery business has been nu by only a few number of companies. But more and more number of companies including medium size ones are participating in the business, and the competition is getting severe. This paper proposes a method to select the optimal location of mail distribution centers that minimally connect the local mail centers of some major cities in Korea using the Steiner Tree theory, which is about connecting a finite number of points with a minimal length of paths and has been used in the distribution system optimization and optimal routing of the transmission lines of the electric power system. By using Steiner Tree theory in finding the best location of the postal delivery hub, we may expect the reduction of transportation cost and the increase of profit, resulting in acquiring the superior position in the competitive delivery business. It is expected that we may use the Steiner Tree theory in finding the best location of the electric power substation for the nott higher EHV(extreme high voltage) transmission network.

Electrical Pulses of Internal Partial Discharges Accompanying with Electrical Tree in LDPE (LDPE에서 발생된 전기트리에 수반된 내부 부분방전 펄스 해석)

  • Kang, S.H.;Park, Y.G.;Kwon, S.S.;Shin, T.S.;Lim, K.J.
    • Journal of the Korean Society of Safety
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    • v.14 no.1
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    • pp.216-221
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    • 1999
  • The correlation between propagation of electrical tree and internal partial discharges is discussed. We use specimens with needle-plane electrode system made of LDPE(Low Density Polyethylene), observed inception and propagation of electrical tree by optical microscope and investigated the characteristics of the partial discharge (PD) pulses accompanying with propagation of electrical tree. In the specimens with needle-shaped void, the tree propagates branch type. The length of the tree has good linear relation with average discharge power of PD pulses. In the specimens without needle-shaped void, the tree grows bush type tree. The correlation between the area of the tree and the average discharge power of PD pulses has linear relation.

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Shooting and Seedling Characteristics according to Growth Regulators, Length and Diameter of Scion in Root Cuttings of Lacquer Tree (Rhus verniciflua) (옻나무 근삽시 생장조절물질과 삽수의 길이 및 직경에 따른 신초발생과 묘소질)

  • 두홍수;권태호;양문식
    • Korean Journal of Plant Resources
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    • v.14 no.1
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    • pp.15-23
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    • 2001
  • Effects of growth regulators, scion length and scion diameter on shooting and seedling characteristics were investigated to increase seedling products by root cuttings of lacquer tree. Shoots developed and emerged above-ground from the root scion at 3∼5 weeks after cut plating, most of that was a shoot but some of that were 2∼3 shoots. Otherwise, non-shooting was the reason that cortical layer was decayed. Soaking in zeatin was the most effective on shooting of root cuttings and high concentration was effective. Also soaking on high contents of BA or kinetin was effective on shooting but was lower totally than soaking on zeatin. Seedling characteristics among 3 growth regulators and 3 concentrations were not any difference in statistical significant, but were move excellent than control. As root scion was tall, shoots were developed at early. 15cm length scion was the most excellent to use seedlings but 3cm length scion was wickedness. 10cm scion, however, was highest to product of seedlings than any others. 0.3∼0.5 cm length scion was showed 76% shooting rate at 10 weeks after cut planting, but seedling characteristics were not any difference among the kind of 3 diameters.

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Mulberry Yields under Several Planting Densities on Hilly Land (경사지 뽕밭에서 식재밀도에 따른 수량의 경년적 변화에 관한 연구)

  • 이종한;김동일
    • Journal of Sericultural and Entomological Science
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    • v.29 no.1
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    • pp.7-11
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    • 1987
  • The influence of planting density, on the mulberry yield, had been studied for 5 years period since 1982. For this purpose, a hilly land (15$^{\circ}$) was selected and 5 planting density level (926, 1,667, 2,780 and 3,444 tree/10a) were used. Following results were obtained. 1. Vinyl mulching promoted 2 days earlier of green tip sprouting stage and 3 days earlier of shallow-bill stage, compared with the non-mulching. 2. It was shown that increase of the length of the branch with vinyl mulching and decrease of the mulching effect with the higher density of plants from, 2,780 tree/10a. 3. When the planting density was higher, length and width of leaf was decreased sharply, and chlorosis of the lower part of the branch was occured heavily. 4. With the higher planting density, number of branches per tree and average length of the branch was decreased. Number of shorter branch-normal branch ratio were increased. However, number of branches and total length of branch per area were shown opposite. 5. Yield of mulberry was increased for the peroid of 3 years after planting with the all the levels of planting density after that, yield increase was dully in 926 tree plot, and more dully in 1,677 trees plot, while decrease of the yield were showen with density of 2,780 and 3,444 trees plots. 6. The highest average yield for the 5 years were obtained from 2,780 trees plot, 46.6% higher than in 926 trees plot, followed 40% higher in 3,444 trees plot, and 29.9% higher in 1,667 trees plot. 7. Soil pH, organic matter, Ca and Mg decreased with cultivation, especially P2O5, pH, Ca and Mg in the density of more than 926 trees plot.

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Tree Biotechnology and Environmental Concerns

  • Kant, Tarun;Emmanuel, C.J.S.K.
    • Journal of Plant Biotechnology
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    • v.6 no.1
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    • pp.1-7
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    • 2004
  • Forestry sector has witnessed some unprecedented events in the recent past both in terms of galloping biotechnological developments and heated environmental debates over risks associated with release of transgenic trees. Improvements in the in vitro propagation techniques has made it possible to develop tissue culture based plant regeneration protocols just for about any tree species. And with the inclusion of every new species within the realms of tissue culture technology, it becomes a candidate for genetic improvement through recombinant DNA technology, the so called genetic engineering. Poplars and their hybrids serve as the model tree species on which most of the genetic transformation work as been carried out. A lot of work has also gone in genetic transformation of fruit trees and trees of horticultural interests. Trees have been successfully transformed for traits ranging from reduction of length of juvenile phase to alteration of tree architecture to altering wood quality by lignin and cellulose modification. More-over trees have been genetically engineered successfully to combat various types of insect pests and pathogens causing diseases. But all these developments have ignited controversies over the possible benefits and risks associated with transgenic plantations by various environmental agencies and activists. Solutions to most of these concerns can be found out with more intensive prioritized research.

A New Low-Skew Clock Network Design Method (새로운 낮은 스큐의 클락 분배망 설계 방법)

  • 이성철;신현철
    • Journal of the Institute of Electronics Engineers of Korea SD
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    • v.41 no.5
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    • pp.43-50
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    • 2004
  • The clock skew is one of the major constraints for high-speed operation of synchronous integrated circuits. In this paper, we propose a hierarchical partitioning based clock network design algorithm called Advanced Clock Tree Generation (ACTG). Especially new effective partitioning and refinement techniques have been developed in which the capacitance and edge length to each sink are considered from the early stage of clock design. Hierarchical structures obtained by parhtioning and refinement are utilized for balanced clock routing. We use zero skew routing in which Elmore delay model is used to estimate the delay. An overlap avoidance routing algorithm for clock tree generation is proposed. Experimental results show significant improvement over conventional methods.