• Nuclear Engineering and Technology
• /
• v.53 no.2
• /
• pp.498-508
• /
• 2021

Micro reactors are increasingly being considered for utilization as distributed power sources. Hence, the probabilistic safety assessment (PSA) of a direct supercritical-CO₂-cooled fast reactor, called micro modular reactor (MMR), was performed in this study; this reactor was developed using innovative design concepts. It adopted a modular design and passive safety systems to minimize site constraints. As the MMR is in its conceptual design phase, design weaknesses and valuable safety insights could be identified during PSA. Level 1 internal event PSA was carried out involving literature survey, system characterization, identification of initiating events, transient analyses, development of event trees and fault trees, and quantification. The initiating events and scenarios significantly contributing to core damage frequency (CDF) were determined to identify design weaknesses in MMR. The most significant initiating event category contributing to CDF was the transients with the power conversion system initially available category, owing to its relatively high occurrence frequency. Further, an importance analysis revealed that the safety of MMR can be significantly improved by improving the reliability of reactor trip and passive decay heat removal system operation. The findings presented in this paper are expected to contribute toward future applications of PSA for assessing unconventional nuclear reactors in their conceptual design phases.

• Biomolecules & Therapeutics
• /
• v.31 no.1
• /
• pp.48-58
• /
• 2023

Interferon regulatory factor 3 (IRF3) integrates both immunological and non-immunological inputs to control cell survival and death. Small GTPases are versatile functional switches that lie on the very upstream in signal transduction pathways, of which duration of activation is very transient. The large number of homologous proteins and the requirement for site-directed mutagenesis have hindered attempts to investigate the link between small GTPases and IRF3. Here, we constructed a constitutively active mutant expression library for small GTPase expression using Gibson assembly cloning. Small-scale screening identified multiple GTPases capable of promoting IRF3 phosphorylation. Intriguingly, 27 of 152 GTPases, including ARF1, RHEB, RHEBL1, and RAN, were found to increase IRF3 phosphorylation. Unbiased screening enabled us to investigate the sequence-activity relationship between the GTPases and IRF3. We found that the regulation of IRF3 by small GTPases was dependent on TBK1. Our work reveals the significant contribution of GTPases in IRF3 signaling and the potential role of IRF3 in GTPase function, providing a novel therapeutic approach against diseases with GTPase overexpression or active mutations, such as cancer.

• Journal of the Korean Society of Systems Engineering
• /
• v.19 no.2
• /
• pp.32-45
• /
• 2023

Implementing Severe Accident Management (SAM) strategies is crucial for enhancing a nuclear power plant's resilience and safety against severe accidents conditions represented in the analysis of Station Blackout (SBO) event. Among these critical approaches, the In-Vessel Retention (IVR) through External Reactor Vessel Cooling (IVR-ERVC) strategy plays a key role in preventing vessel failure. This work is designed to evaluate the efficacy of the IVR strategy for a high-power density reactor APR1400. The APR1400's plant is represented and simulated under steady-state and transient conditions for a station blackout (SBO) accident scenario using the computer code, ASYST. The APR1400's thermal-hydraulic response is analyzed to assess its performance as it progresses toward a severe accident scenario during an extended SBO. The effectiveness of emergency operating procedures (EOPs) and severe accident management guidelines (SAMGs) are systematically examined to assess their ability to mitigate the accident. A group of associated key phenomena selected based on Phenomenon Identification and Ranking Tables (PIRT) and uncertain parameters are identified accordingly and then propagated within DAKOTA Uncertainty Quantification (UQ) framework until a statistically representative sample is obtained and hence determine the uncertainty bands of key system parameters. The Systems Engineering methodology is applied to direct the progression of work, ensuring systematic and efficient execution.

• Journal of Drive and Control
• /
• v.21 no.3
• /
• pp.9-19
• /
• 2024

Many modern hydraulic excavators now use EPPRVs (Electronic Proportional Pressure Reducing Valves) in their pilot systems to control the spool displacement of the main hydraulic system. However, the performance of these systems is often limited by factors such as magnetic hysteresis, mechanical wear, and transient responses influenced by operating conditions and component installation. This paper presents a pressure feedback controller for excavator pilot systems that utilize EPPRVs. This controller significantly reduces steady-state pressure control errors and mitigates the hysteresis effects commonly seen in traditional open-loop systems. To achieve this, we integrated EPPRVs with the main hydraulic valve and injected a chirp signal into the solenoid current. By doing so, we were able to measure the frequency response of the pilot system across different operating pressures and estimate the system dynamics model. Using these models, we designed a set of PI pressure feedback controllers that are guaranteed to be stable. These controllers were then integrated with a gain scheduler based on a lookup table. Experimental results demonstrate that when the developed pressure feedback controller is incorporated into the conventional open-loop controller, it effectively reduces steady-state pressure control errors and mitigates hysteresis.

• Applied Biological Chemistry
• /
• v.38 no.5
• /
• pp.387-392
• /
• 1995

To study the regulatory expression mechanism of soybean glycinin gone, Gy2, the 5' upstream region of the gene was searched for the presence of putative regulatory elements by nucleotide sequencing. It revealed various kinds of regulatory sequence elements commonly found in plant storage protein genes. There were canonical promoter sequences, TATA box (TATAAT) and AGGA box (GAAT) which are common in the 5' upstream region of the plant genes. The embryo factor binding sequence, RY repeat, CACA sequences, ${\alpha}$-conglycinin enhancer-like sequences were also found. To delineate the function of these sequences, 5' upstream deletion mutants of Gy2 were prepared and fused to the ${\alpha}$-glucuronidase (GUS) gene. Each chimeric construct was transferred into soybean protoplasts for transient assay, which led to the identification of the sequences between -281 and -223, -170 and -122, of Gy2 promoter as negative regulatory elements, and the sequences between -223 and -170, -122 and -16 as positive regulatory elements. These results are consistent in transformed tobacco plants as well. The serially deleted promoter fragments fused to the GUS were transformed into Nicotiana tabacum by Agrobacterium tumefaciens using the binary vector system. GUS activity of Gy2 promoter deletion constructs was detected only in seeds but not in leaves with different levels of expression as in transient assay. These results suggest that the glycinin Gy2 promoter drives a tissue-specific expression in transgenic tobacco plants.

• Proceedings of the Korea Society of Environmental Toocicology Conference
• /
• 2003.05a
• /
• pp.107-107
• /
• 2003

The heavy metal cadmium is a xenobiotic toxicant of environmental and occupational concern and it has been classified as a human carcinogen. Inhalation of cadmium has been implicated in the development of emphysema and pulmonary fibrosis, but, the detailed mechanism by which cadmium induces adverse biological effects is not yet known. Therefore, we undertook the investigation of genes that are induced after cadmium exposure to illustrate the mechanism of cadmium toxicity For this purpose, we employed the polymerase chain reaction-based suppression subtractive hybridization technique. We identified 29 different cadmium-inducible genes in human peripheral mononuclear cells, such as macrophage migration inhibitory factor, lysophosphatidic acid acyltransferase-${\alpha}$, enolase-1${\alpha}$, VEGF, Bax, neuron-derived orphan receptor-1, and Nur77, which are known to be associated with inflammation, cell survival, and apoptosis. Induction of these genes by cadmium treatment was further confirmed by semi-quantitative reverse-transcription polymerase chain reaction. Further, we found that these genes were also induced after cadmium exposure in normal human lung fibroblast cell line, WI-38, suggesting potential use of this induction profile to monitor cadmium toxicity in the lung. Next, Nur77, one of cadmium-inducible genes, was further studied since the products of Nur77 are known to be involved in the apoptotic process of lung cells. Following cadmium treatment, Nur77 gene expression was increased at protein-level in A549 cells. Consistently, the reporter containing Nur77 binding sequence was activated by 2.5-fold after exposure to cadmium in reporter gene analysis by transient transfection experiments. When the plasmid encoding dominant negative Nur77 that represses the transcriptional function of wild-type Nur77 was transfected into A549 cells, the expression of Bax was significantly reduced, suggesting that induction of Nur77 was an important process in cadmium-induced apoptosis in the cells. Cadmium induced the expression of Nur77 in vivo, confirming the relevance of the data obtained in viro. Together our results suggest that Nur77 gene expression in exposure to cadmium leads apoptosis of lung cells which may cause pathological changes in lung.

• Journal of Communications and Networks
• /
• v.11 no.6
• /
• pp.544-555
• /
• 2009

This work continues a trend of developments aimed at exploiting the physical layer of the open systems interconnection (OSI) model to enhance wireless network security. The goal is to augment activity occurring across other OSI layers and provide improved safeguards against unauthorized access. Relative to intrusion detection and anti-spoofing, this paper provides details for a proof-of-concept investigation involving "air monitor" applications where physical equipment constraints are not overly restrictive. In this case, RF fingerprinting is emerging as a viable security measure for providing device-specific identification (manufacturer, model, and/or serial number). RF fingerprint features can be extracted from various regions of collected bursts, the detection of which has been extensively researched. Given reliable burst detection, the near-term challenge is to find robust fingerprint features to improve device distinguishability. This is addressed here using wavelet domain (WD) RF fingerprinting based on dual-tree complex wavelet transform (DT-$\mathbb{C}WT$) features extracted from the non-transient preamble response of OFDM-based 802.11a signals. Intra-manufacturer classification performance is evaluated using four like-model Cisco devices with dissimilar serial numbers. WD fingerprinting effectiveness is demonstrated using Fisher-based multiple discriminant analysis (MDA) with maximum likelihood (ML) classification. The effects of varying channel SNR, burst detection error and dissimilar SNRs for MDA/ML training and classification are considered. Relative to time domain (TD) RF fingerprinting, WD fingerprinting with DT-$\mathbb{C}WT$ features emerged as the superior alternative for all scenarios at SNRs below 20 dB while achieving performance gains of up to 8 dB at 80% classification accuracy.

• Journal of Korean Neurosurgical Society
• /
• v.46 no.4
• /
• pp.340-345
• /
• 2009

Objective : We investigated the locations of compressing vessels in hemifacial spasm. To approach compression sites, we described and evaluated the efficacy of the infranuchal infrafloccular (INIF) approach. Methods : A retrospective review of 31 consecutive patients who underwent microvascular decompression (MVD) through INIF with a minimum follow-up of 1 year was performed. Along the intracranial facial nerve, we classified the compression sites into the transitional zone (TRZ), the central nervous system (CNS) segment and the peripheral nervous system (PNS) segment. The INIF approach was used to inspect the CNS segment and the TRZ. Subdural patch graft technique was used in order to achieve watertight dural closure. The cranioplasty was performed using polymethylmethacrylate. The outcome and procedure-related morbidities were evaluated. Results : Twenty-nine patients (93%) showed complete disappearance of spasm. In two patients, the spasm was resolved gradually in 2 and 4 weeks, respectively. Late recurrence was noted in one patient (3%). The TRZ has been identified as the only compression site in 19 cases (61.3%), both the TRZ and CNS segment in 11 (35.5%) and the CNS segment only in 1 (3.2%). There was no patient having a compressing vessel in the PNS segment. Infection as a result of cerebrospinal fluid leak occurred in one patient (3%). Delayed transient facial weakness occurred in one patient. Conclusion : The TRZ and the CNS segment were more vulnerable area to the compression of vessels. We suggest that surgical avenue with the INIF approach provides early identification of this area.

• Journal of Korean Neurosurgical Society
• /
• v.54 no.2
• /
• pp.86-92
• /
• 2013

Objective : Surgical results for anterior choroidal artery (AChA) aneurysms have previously been reported as unsatisfactory due to inadvertent occlusion of the AChA, while the low incidence of AChA aneurysms hampers the accumulation of surgical experience. The authors reviewed their related surgical experience to document the ischemic complications and surgical outcomes. Methods : Identification of the AChA at its origin by rigorous visual scrutiny, careful microdissection, and meticulous clip placement to avoid the AChA origin are all crucial surgical maneuvers. A retrospective review of a surgical series of 62 consecutive cases of an AChA aneurysm between 2004 and 2012 was performed. Results : All patients, except for five (8.1%) with a small residual neck, showed complete aneurysm obliteration in postoperative angiographic evaluations. There was no incidence of procedure-related permanent AChA syndrome or oculomotor nerve palsy, while three (4.8%) patients suffered from transient AChA syndrome. The clinical outcomes [the 3-month modified Rankin Scale (mRS)] of the patients were related to their preoperative World Federation of Neurologic Surgeons (WFNS) grade. As regards the 3-month mRS, significant differences were found between patients with an unruptured aneurysm (WFNS grade 0; n=20), good-grade subarachnoid hemorrhage (WFNS grade 1-3; n=30), and poor-grade subarachnoid hemorrhage (WFNS grade 4-5; n=4). Conclusion : In surgical treatment of AChA aneurysms, a risk of AChA insufficiency can be minimized by taking every precaution to preserve the AChA patency and intraoperative monitoring. In addition, the surgical outcome is primarily determined by the preoperative clinical state.

• Transactions of the Korean Society of Automotive Engineers
• /
• v.22 no.2
• /
• pp.60-66
• /
• 2014

A control oriented model of the Lean $NO_x$ trap (LNT) was developed to determine the timing of $NO_x$ regeneration. The LNT model consists of $NO_x$ storage and reduction model. Once $NO_x$ is stored ($NO_x$ storage model), at the right timing $NO_x$ should be released and then reduced ($NO_x$ reduction model) with reductants on the catalyst active sites, called regeneration. The $NO_x$ storage model simulates the degree of stored $NO_x$ in the LNT. It is structured by an instantaneous $NO_x$ storage efficiency and the $NO_x$ storage capacity model. The $NO_x$ storge capacity model was modeled to have a Gaussian distribution with a function of exhaust gas temperature. $NO_x$ release and reduction reactions for the $NO_x$ reduction model were modeled as Arrhenius equations. The parameter identification was optimally performed by the data of the bench flow reactor test results at space velocity 50,000/hr, 80,000/hr, and temperature of $250-500^{\circ}C$. The LNT model state, storage fraction indicates the degree of stored $NO_x$ in the LNT and thus, the timing of the regeneration can be determined based on it. For practical purpose, this model will be verified more completely by engine test data which simulate the NEDC transient mode.