• Reproductive and Developmental Biology
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• 제35권3호
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• pp.251-256
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• 2011

Human growth hormone (hGH), one of the most important hormones in medicine, is secreted from anterior pituitary gland. Its broad physiological function includes body growth, cell regeneration, increasement of muscle volume, bone density, body fat reduction, and so on. Due to the wide range of therapeutic effects, the hGH produced from E. coli has been commercialized already. In this study, we asked whether it is possible to produce recombinant hGH efficiently from various cultured mammalia cells. To meet this purpose, we chose a retrovirus vector system for transfer and expression of the hGH gene in various mammalian cells. Analyses of RT-PCR, ELISA, and Western blot to determine expression of the hGH gene showed the highest production of the hGH was determined from chicken embronic fibroblast (CEF) cells with the concentration of 8.58 ${\mu}g$/ml. The biological activity of the hGH was similar to the commercially available counterpart. These results suggest that mass production of hGH is possible not only in the E. coli but also in the various mammalian cells.

• Reproductive and Developmental Biology
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• 제40권1호
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• pp.7-13
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• 2016

Effectiveness of transgene transfer into genome is crucially concerned in mass production of the bio-pharmaceuticals using genetically modified transgenic animals as a bioreactor. Recently, the mammary gland has been considered as a potential bioreactor for the mass production of the bio-pharmaceuticals, which appears to be capable of appropriate post-translational modifications of recombinant proteins. The mammary gland tissue specific vector system may be helpful in solving serious physiological disturbance problems which have been a major obstacle in successful production of transgenic animals. In this study, to minimize physiological disturbance caused by constitutive over-expression of the exogenous gene, we constructed new retrovirus vector system designed for mammary gland-specific expression of the hEPO gene. Using piggyBac vector system, we designed to express hEPO gene under the control of mammary gland tissue specific and lactogenic hormonal inducible goat ${\beta}$-casein or mouse Whey Acidic Protein (mWAP) promoter. Inducible expression of the hEPO gene was confirmed using RT-PCR and ELISA in the mouse mammary gland cells treated with lactogenic hormone. We expect the vector system may optimize production efficiency of transgenic animal and reduce the risk of global expression of transgene.

• 미생물학회지
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• 제42권1호
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• pp.73-76
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• 2006

신정전구세포를 이용한 퇴행성 뇌질환의 세포치료나 유전자치료에서 효율적인 유전자 전달을 목적으로 개량된 아데노바이러스 벡터의 실용 가능성을 쥐의 해마에서 유래된 신정전구세포를 이용하여 조사하였다. 외피단백질을 조작한 개략 아데노바이러스벡터는 분화전과 후의 신정전구세포로 1세대 아데노바이러스 벡터에 비해 6배 정도 유전자를 효율적으로 전달하였다. 또한 바이러스의 감염은 신정전구세포가 신경세포나 신정 아교세포로 분화하는데 영향을 미치는 않았다. 따라서 신정전구세포를 이용한 신정질환의 세포치료나 유전자 치료에서 개량된 아데노바이러스로 유전자를 전달하면 치료의 효율성을 향상시킬 수 있을 것이다.

• 대한바이러스학회지
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• 제29권1호
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• pp.33-38
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• 1999

Retroviral vector provide a highly efficient method for gene transfer into eukaryotic cells. This vector system can be divided into two components; the retroviral vector itself and the retroviral packaging cell line. The key improvement in the design of these two components are, focused on two aspects; the reduction of helper virus production and high titer-virus. We used PA317 for retrovirus packaging cell line, for its high producibility of viral titer. To test the ability of the vectors to generate high titer-virus, we have chosen four different retroviral vectors; LN, LNSX, LNCX and LXSN. To test easily the viral titer, we have made recombinant construction with CD4 and CD8, checked their viral titer and stained their surface expression. LXSN which contain SV40 early promoter in front of neo gene showed best results in viral transient transfection assay, dot blot assay and surface expression. In addition, recombinant containing CD8 generally showed much higher viral titration and surface expression than CD4.

• 한국음향학회지
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• 제34권2호
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• pp.108-116
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• 2015

수중 음향 벡터센서는 음압 뿐 아니라 음파의 진행 방향에 관한 정보를 측정할 수 있는 센서이다. 본 논문에서는 미세 외팔보를 이용한 수중 음향 벡터 센서를 구현하기 위해 음향학적 이론을 바탕으로 음향과 기계 구조물의 상호작용을 이론적으로 정립하고자 하였다. 감응 방식으로 압전 효과를 이용한 두 가지 유니모프(unimorph)형태의 모델을 제시하였으며, 제시된 모델에 대하여 압전 미세 외팔보의 거동을 집중 질량 모델을 통해 음파가 임의의 주파수와 각도를 가지고 미세 외팔보로 입사할 때 나오는 신호의 크기를 구할 수 있는 전달함수를 유도하였다. 또한 이를 바탕으로 매우 얇고 유연한 구조물로 미세 외팔보를 설계하면 매질의 입자 속도에 관한 정보를 직접적으로 측정 가능한 센서로 활용할 수 있다는 것을 확인하였다.

• Journal of Power Electronics
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• 제5권4호
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• pp.289-304
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• 2005

This paper describes a simple control structure and power conditioning system for an indirect vector controlled stand-alone induction generator (IG) used to operate under variable speed. The required reactive power for the IG system is supplied by means of a capacitor bank and a voltage-source PWM converter. Using a capacitor bank to transfer the reactive power to the IG under the rated speed and no-load conditions starts the IG operation and reduces the PWM converter size. The vector control structure for the variable speed IG power conditioning system compensates for changes in the electrical three-phase and DC loads while considering the magnetizing curve of the IG. The vector control structure is developed to regulate the DC link voltage of the PWM converter and the IG output voltage. The experimental and simulated performance results of the IG power conditioning system at various speeds and loads are given and show that this proposed scheme can be used efficiently for a variable speed, wind energy conversion system.

• International Journal of Industrial Entomology and Biomaterials
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• 제3권1호
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• pp.75-81
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• 2001

We have constructed a novel recombinant Bombyx mori nuclear polyhedrosis virus (BmNPV) producing the green fluorescent polyhedra. For the production of the fluorescent polyhedra, partial polyhedrin gene containing KRKK as nuclear localization site from the BmNPV polyhedrin gene and the green fluorescent protein (gfp) gene were introduced under the control of p10 promoter of BmNPV. The recombinant BmNPV was stably produced fluorescent polyhedra in the infected Bm5 cells and the morphology of the fluorescent polyhedra was similar to that of wild-type BmNPV. The fluorescent polyhedra had 32 kDa native polyhedrin and 41 kDa fusion protein. From these data, we have further developed a novel BmNPV p10-based transfer vector producing recombinant polyhedra with foreign gene Product. The novel BmNPV P10-based transfer vector is composed of partial polyhedrin gene, factor Xa, and multiple cloning sites.

• 천문학논총
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• 제10권1호
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• pp.91-108
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• 1995

We have intensively examined the structure of photospheric magnetic fields obtained from the calculation of the polarized radiation transfer for the model atmosphere. To determine more reliable magnetic field in the photospheric region composed of umbra, penumbra and quite area, we have calculated the polarized radiative transfer for a magnetically sensitive spectral line, FeI $6302.5{\AA}$, using our composite model representing three kinds of the atmospheric area distinguished by the pixel value of the Stokes I image over the region. Polarization data of the full Stokes parameters, used in this paper had been obtained from the vector magnetograph on Solar Flare Telescope of National Astronomical Observatory at Mitaka(MTK) in Japan. According to our investigation on the active region in the photosphere, it has been found that the large current density(${\geq}8{\times}10^2A/km^2$) and shear angle(${\geq}85^{\circ}$) should be distributed along the magnetic neutral line. To be compared with the results of MTK, our results in transverse magnetic field strength and direction are similar with those of MTK, however our longitudinal field strength at the center of the spot is somewhat(${\sim}1000$ Gauss) larger than MTK.

• KSII Transactions on Internet and Information Systems (TIIS)
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• 제14권1호
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• pp.1-19
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• 2020

In an underlay cognitive simultaneous wireless information and power transfer (SWIPT) network, communication from secondary user (SU) to secondary destination (SD) is accomplished with decode-and-forward (DF) relays. Multiple energy-constrained relays are assumed to harvest energy from SU via power splitting (PS) protocol and complete SU secure information transmission with beamforming. Hence, physical layer security (PLS) is investigated in cognitive SWIPT network. In order to interfere with eavesdropper and improve relay's energy efficiency, a destination-assisted jamming scheme is proposed. Namely, SD transmits artificial noise (AN) to interfere with eavesdropping, while jamming signal can also provide harvested energy to relays. Beamforming vector and power splitting ratio are jointly optimized with the objective of SU secrecy capacity maximization. We solve this non-convex optimization problem via a general two-stage procedure. Firstly, we obtain the optimal beamforming vector through semi-definite relaxation (SDR) method with a fixed power splitting ratio. Secondly, the best power splitting ratio can be obtained by one-dimensional search. We provide simulation results to verify the proposed solution. Simulation results show that the scheme achieves the maximum SD secrecy rate with appropriate selection of power splitting ratio, and the proposed scheme guarantees security in cognitive SWIPT networks.

• Journal of Pharmaceutical Investigation
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• 제34권5호
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• pp.351-362
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• 2004

The basic concept underlying gene therapy is that human diseases may be treated by the transfer of genetics material into specific cells of a patient in order to correct or supplement defective genes responsible for disease development. There are several systems that can be used to transfer foreign genetic material into the human body. Both viral and non-viral vectors are developed and evaluated for delivering therapeutic genes. Viral vectors are biological systems derived from naturally evolved viruses capable of transferring their genetics materials into host cells. However, the limitations associated with viral vectors, in terms of their safety, particularly immunogenecity, and their limited capacity of transgenic materials, have encouraged researchers to increasingly focus on non-viral vectors as an alternative to viral vectors. Although non-viral vectors are less efficient than viral ones, they have the advantages of safety, simplicity of preparation and high gene encapsulation capability. This article reviews the most recent studies highlighting the advantages and the limitation of gene delivery systems focused on non-viral systems compared to viral systems.