• 대한한방내과학회지
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• 제1권1호
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• pp.35-43
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• 1976

The effects of root of the Bupleurum falcatum L. on the serum transaminase (SGP-T, SGO-T) activities and the content of total cholesterol in serum from normal and alloxan-diabetic rabbits were observed. The preparations were extracted by methanol, and extracted samples were orally administrated to the normal and alloxandiabetic rabbits. The transaminase activities and the content of total cholesterol in serum of normal rabbits were significantly increased by administration of the alloxan. The increased serum transaminase activities of the alloxandiabetic rabbits were significantly decreased after 96 hours by administration of the extracted samples. The increased content of total cholesterol in serum of the alloxan-diabetic rabbits were gradually decreased by administration of the extracted samples and were recovered to the control levels after 12 days. According to the above results, it would be concluded that the extract of Bupleuri Radix influences for the recovery of the increased contents of total cholesterol and serum transaminase activities by alloxan.

• 한국환경보건학회지
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• 제4권1호
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• pp.23-25
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• 1977

Transaminase has been a popular methods in the clinical laboratory for the diagnosis of particular diseases. The methods we are familiar with should be considered further on the serum states provided on the test to be done. (serum freshness, temperature and duration preserved) I have studied transaminase activities of the serum preserved under frozen (-10$\circ$C), refrigerated (4$\circ$C) and room temperature conditions. At a given interval those activities of serum are measured and then the results are compared with the optical density and calculated for the standard deviation and percent variation (Table 1. & Fig. 1.) G.O.T. activity is maintained decreased at a relatively constant rate from 2nd day to 14th day preservation under frozen and refrigerated but its activity variation at 25$\circ$C are far greater than the above conditions throughout the test periods. Elevated G.P.T. level of serum compared with G.O.T. are remained at any temperature and duration. Unless the determination of amino acid level study can not ruled out clearly.

• 생명과학회지
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• 제9권4호
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• pp.375-381
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• 1999

To evaluate the protective effects of extracts of silkworm on carbon tetrachloride-induced hepatotoxicity, serum glutamic-oxaloacetic transaminase, glutamic-pyruvic transaminase and lactic dehydrogenase activities, malondialdehydes values and glutathione-S-transferase activity were measured in ICR mice. Extracts of silkworm was administered orally at 30min after the administration of CCl4 Mice were sacrificed at 24h after the administration of extracts of silkworm. The activities of serum aminotransferase and the hepatic content of lipid peroxide after carbon tetrachloride treatment were markedly increased than normal control but those levels were decreased by the treatment of butanol soluble fraction of silkworm methanol extract. Glutathione S-transferase activity was decreased by carbontetrachloride than control, but also inhibited by the treatment of butanol soluble fraction of silkworm methanol extract.

• 생약학회지
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• 제16권1호
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• pp.1-6
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• 1985

Effect of various fractions from the roots of Patrinia scabiosaefolia (Valerianaceae) and whole plants of Melandryum firmum (Caryophyllaceae) on enzyme activities in mice was investigated. The butanol fractions from both plants caused a significant elevation of serum transaminase activities when administered intraperitoneally, but did not, orally. Prolonged exposure by oral administration of both plants elevated hepatic cytochrome p-450 content, indicating the induction of drug metabolizing enzymes in liver.

• 한국식품영양과학회지
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• 제29권2호
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• pp.193-197
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• 2000

The effects of black soybena extracts on enzymes activies of rat were evaluated in present study. Eighty-four male Sprague-Dawley rats weighing 100$\pm$10g were divided into twelve groups which consisted of black soybean extract, Pb and Cd solution, and black soybean extract plus Pb or Cd soln groups. The weight gain was increased in black soybean extracts and Pb soln solution group but decreased in Cd soln solution group. The results obtained form the experiment were as follows: Glutamate pyruvate trasaminase (GPT) and glutamate oxaloacetate oxaloacetate transaminase (GOT) activities were not significantly different among experimental groups. The lactate dehydrogenase (LDH) activities of black soybean extract administered groups were decreased than those of Pb and Cd solution group. Black soybean group increased cholinesterase (ChEase) activity as compared to administration of Pb and Cd soln group.

• 대한약리학회지
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• 제9권1호
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• pp.47-51
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• 1973

A benzomorphan derivative, pentazocine has both opioid agonistic actions and weak narcotic antagonistic activity. In this paper, authors attempted to study the dose response of pentazocine on the blood sugar level, serum transaminase and alkaline phosphatase activity in rabbits. Eighteen rabbits were devided into 3 groups, and each group were injected with pentazocine 5mg, 15mg, and 30mg/kg respectively. And metabolic effects were investigated measuring the change of blood sugar contents, serum transaminase (S-GOT, S-GPT) activities and alkaline phosphatase activities in rabbits. The results were obtained as follows; 1. Pentazocine significantly increased the blood sugar content in rabbits. 2. Pentazocine significantly increased the serum GOT activity but in the serum GPT activity, it significantly increased in large dose. 3. Pentazocine significantly increased serum alkaline phosphatase activity.

• 한국응용곤충학회지
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• 제15권4호
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• pp.169-178
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• 1976

솔잎혹파리 (Thecodiplosis japonensis Uchi. et Inouye)의 충영속 유충과 충영을 탈출하여 낙하한 토중의 유충을 대상으로 체액을 분석, 측정코저 종이 크로마토그라피법, micro-Kjeldahl법, 박층 크로카토그라피 법, 왈부르그 검압계법, Bessey-Lowry법과 Reitman-Frankel법으로 측정 또는 분석하였다. 충영과 유충과 토중의 유충에 따라 유리 아미노산, 총 질소함량의 변화, 탄수화물의 변화, 지질의 종류와 변화, 호흡능, phosphatase, GOT, ?의 활성도가 측정되었다. 유리 아미노산, 총 질소함량, 지질함량, 호흡능, acid phosphatase의 황성, GPT의 활성은 충영속의 유충에서 토중의 유충으로 진행함에 따라 감소하는 경향을 나타냈다. 그러나 trehalose의 함량과 alkaline phosphatase의 활성은 증가하는 경향을 나타냈다.

• 한국동물학회지
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• 제17권3호
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• pp.131-138
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• 1974

成熟한 Sprague-Dawley 系의 雄性흰쥐를 $30\\pm 0.5^\\circ C$에서 240時間 그리고 $33\\pm 0.5^\\circ C$에서 64時間 各各 溫熱曝露시켰으며, 溫熱曝露 期間中 여러 時間區에서 血淸 glutamic oxaloacetic transaminase (GOT)와 血淸 glutamic pyruvic transaminase (GPT)의 活性을 Reitman 및 Frankel (1957)의 方法에 依해서 Coleman-Model 295E Spectrophotometer로서 各各 550m$\\mu$에서 測定하였다. $23\\pm 1^\\circ C$인 對照群에 比較하여 보면, $30\\pm 0.5^\\circ C$ 및 $33\\pm 0.5^\\circ C$에서 溫熱曝露된 흰쥐는 各各 血淸의 GOT 및 GPT의 活性이 有意性있게 上昇되었다. 서로 다른 高溫의 溫熱에 曝露된 흰쥐의 血淸 GOT 活性은 16 및 30時間 區間中에서 또한 64 및 72時間 區間中에서 各各 有意性있게 上昇되었다. 한편 $30\\pm 0.5^\\circ C$에서 72時間後에는 血淸 GOT 活性은 正常群으로 되돌아가는 傾向이 나타났다. 서로 다른 高熱의 溫熱로 曝露된 흰쥐의 血淸GPTdml 活性은 初期인 4時間區에서 有意性있게 上昇되었으며, 이어서 16時間區와 64 및 72時間 區間中에서 各各 上昇되었다. 한편 $30\\pm 0.5^\\circ C$에서는 114時間後에 약간 增加하는 傾向이 나타났다. $30\\pm 0.5^\\circ C$와 $33\\pm 0.5^\\circ C$에서 各各 溫熱曝露된 흰쥐의 酵素活性을 比較하여 보면, $33\\pm 0.5^\\circ C$에 曝露된 흰쥐의 血淸 GOT 및 血淸 GPT의 活性은 各各 16, 30 및 64時間區에서 有意性있게 높은 活性을 나타내었다. 이러한 結果로 미루어 보아 連續的 溫熱曝露는 非正常의 아미노基 轉移代謝를 招來하는 것으로 思料된다.

• Archives of Pharmacal Research
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• 제26권1호
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• pp.43-46
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• 2003

The anti-oxidant activities of tectochrysin, a major compound of propolis, were investigated. Tectochrysin exhibited a significant decrease in serum transaminase activities elevated by hepatic damage induced by $CCl_4$-intoxication in rats. Tectochrysin tested exhibited a lipid peroxidation causing a significant decrease in MDA production in TBA-reactant assay. Tectochrysin was strong in the increase in the anti-oxidant enzymes such as hepatic cytosolic superoxide dismutase, catalase and glutathione peroxidase activities in $CCl_4$-intoxicated rats. These results suggest that tectochrysin possess not only the anti-oxidant, but also the activities in $CCl_4$-intoxicated rats. Especially, tectochrysin was found to cause significant increases in the rat liver cytosolic SOD, catalase, GSH-px activities as well as a significant decrease in the MDA production.

• 한국유기농업학회지
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• 제9권3호
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• pp.85-92
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• 2001

The effect of Dolichos lablab leave extract on enzyme activities in mice blood was investigated in this study to clarify the new useful application of the Plant leaves. There were not significant differences in the enzyme activities in mice blood among treatment fed with the leave extract and non fed control. The feeding treatment with the extract showed a tendency to activity compared to the nun fed control. The activities of glutamate oxaloacetate transaminase, glutamate pyruvate transaminase and lactate dehydrogenase in mice blood at the treatment fed with lead and the leave extract were significantly low compared to their activities at the non fed treatment with the extract, respectively. The choline esterase activity was high at the leave extract feeding. The cadmium dietary treatment showed the same result as the lead treatment. In conclusion, the physiological function of the Jebikong leave was significantly in creased when the mice was stressed by the hear metal intake. Therefore, the plant leave extract would consider the reduction of heart metal effect.