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Development of Hepatocyte Spheroids Immobilization Technique Using Alternative Encapsulation Method

  • Kim, Sungd-Po;Lee, Doo-Hoon;Park, Jung-Keug
    • Biotechnology and Bioprocess Engineering:BBE
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    • v.3 no.2
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    • pp.96-102
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    • 1998
  • Primary hepatocytes of small animals such as rat and rabbit were often used for the study of extracorporeal liver support systems. Freshly isolated rat hepatocytes form spheroids within tow days when cultivated as suspension in spinner vessels. These spheroids showed enhanced liver specific functions and more differentiated morphology compared to hepatocytes cultured as monolayers However, shear stress caused by continuous agitation deteriorated spheroids gradually. In this work we immobilized spheroids to prolong liver specific activities. First, hepatocyte spheroids were suspended in collagen solution containing calcium chloride and then dropped into alginate solution. A thin layer of calcium alginate was formed around the droplet and then was removed after the inner collagen was gelled by treatment of sodium citrate buffer. Spheroids embedded in collagen-gel bead maintained liver specific functions such as albumin secretion rate longer than hepatocyte spheroids exposed to shear stress. Therefore, we suggest that this immobilization technique may offer an effective long-term hepatocyte cultivation and facilitase the development of a bioartificial liver support device.

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CDMA Pilot Receiving Circuit Using Sequence Estimator (시퀀스 추정기를 사용하는 CDMA 파일럿 수신회로)

  • Lee, Seong-Min
    • Journal of the Korea Institute of Military Science and Technology
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    • v.9 no.4
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    • pp.32-38
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    • 2006
  • In this paper a sequence estimator of CDMA communication system is suggested. A sequence estimator uses Galois Field operation. A sequence estimator can provide another CDMA pilot signal which is un-modulated spreaded signal. A estimated sequence signal and received signal have no correlation. Tow signals can be summed using MRC(maximal ratio combine) method. The stronger signal can be added as a larger ratio, but the weaker signal can be added as a smaller ratio. We can distinguish strong signal using SNR estimator. Therefore it is possible to receive an additional pilot signal, and to support more reliable communications by using sequence estimator.

A Plan for Reducing SAR Call-outs for Fishing Vessels in Korean Waters

  • Smith, Matthew V.
    • Journal of Navigation and Port Research
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    • v.39 no.2
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    • pp.83-88
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    • 2015
  • Korea has a systemic problem with lack of engine maintenance, especially among the Commercial Fishing Vessel fleet. This results in a inordinate percent of SAR call-outs for vessels with engine failure. These SAR call-outs lead to a free tow to shore by KCG or by one of its volunteer associates. Although these tows are not a terrible economic burden on KCG, it is a burden in terms of time and resources mis-allocated. This paper proposes a Commercial Fishing Vessel Examination (CFVE) program modeled after the program run by USCG. It is expected that adopting the CFVE program, KCG may be able to create a culture of safety among fishermen; thus, replicating the USCG's success rates.

LPE GROWTH OF $La_{2-x}Sr_xCuO_4$ SINGLE-CRYSTALLINE FILMS

  • Tanaka, Isao;Tanabe, Hideyoshi;Watauchi, Satoshi;Kojima, Hironao
    • Proceedings of the Korea Association of Crystal Growth Conference
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    • 1999.06a
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    • pp.371-387
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    • 1999
  • La2-xSrxCuO4 single-crystalline films were prepared on bulk single crystals of Zn-doped La2CuO4 as the substrates by LPE technique using tow deferent methods. When prepared using an alumina crucible in normal electrical furnace, the La2-xSrxCuO4 films were contaminated with less than 3 at% aluminum from the alumina crucibles. Aluminum contamination either reduced or completely destroyed the superconductivity of the La2-xSrxCuO4 films. For LPE growthby modified TSFZ method using an infrared heating furnace without crucibles, the La2-xSrxCuO4 films of x=0.11 showed superconducting with Tconset 36 K, which is 10 K higher than that in the La2-xSrxCuO4 bulk single crystals.

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Effect of SiC mean particle size on mechanical properties and microstructure of $Si_{3}N_{4}/SiC$ nanocomposites (SiC 입자크기가 $Si_{3}N_{4}/SiC$ 초미립복합재료의 기계적 특성과 미세구조에 미치는 영향)

  • 황광택;김창삼;정덕수;오근호
    • Journal of the Korean Crystal Growth and Crystal Technology
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    • v.6 no.3
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    • pp.392-398
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    • 1996
  • $Si_{3}N_{4}/SiC$ nanocomposites reinforced with tow different mean particle size were fabricated by hot press. Grain growth of matrix gran was inhibited by adding of SiC particles, and then number of equiaxed and fine grains were increased. The effect of grain growth inhibition was higher in the nanocomposites dispersed small size SiC. herefore fracture strength and hardness were increased, but fracture toughness was decreased in small size SiC dispersed samples.

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A Single-Stage PFC AC/DC Forward Converter With Semi-automatic Current Shaping (전류 불연속 모드로 동작하는 1단 방식의 역률 보상 AC/DC 포워드 컨버터)

  • 강필순;김원호;박성준;김철우
    • Proceedings of the KIPE Conference
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    • 1999.07a
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    • pp.319-322
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    • 1999
  • This paper presents a novel single-stage Power Factor Corrected(PFC) AC/DC forward converter with semi-automatic current shaping in order to achieve the unity power factor and an isolated output. Since the proposed circuit is combined a boost converter used for PFCs with a forward converter used for DC to DC conversion, the over-all size of system could be reduced. And thanks to the zero voltage switching(ZVS) in both switches, the voltage stress can be reduced considerably. A simple auxiliary circuit adopted into the secondary of transformer is composed of lossless components for reducing surge voltage. A prototype which has tow IGBT(Insulated Gate Bypolar Transistor) modules as switching device is manufactured to evaluate the proposed topology. The characteristics of the proposed circuit are tested, and the validity is verified by experimental results.

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A study on SFCL systems for power system application (실계통적용을 위한 초전도한류시스템에 대한 연구)

  • Lee Seung Ryul;Kim Jong Yul;Yoon Jae Young
    • Progress in Superconductivity and Cryogenics
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    • v.7 no.1
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    • pp.51-56
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    • 2005
  • The recovery time of developing SFCL(Superconducting Fault Current Limiter) has an uncertainty. In general, the recovery time is estimated at 1 sec and more, even though the Process of SFCL technology is considered. However, auto reclosing time of circuit breaker is 0.3 sec in Korean power system. It is impossible to apply only one SFCL to power system because the recovery time is over the reclosing time of protection system. This study proposes two new SFCL systems for power system application. The proposed systems consider auto reclosing action for the protection in practical power system and consist of tow parallel SFCLS.

A Dynamic Response Analysis of Tension Leg Platforms in Waves (I) (인장계규식 해양구조물의 동적응답해석(I))

  • 구자삼;김진하;이창호
    • Journal of Ocean Engineering and Technology
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    • v.9 no.1
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    • pp.161-172
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    • 1995
  • A numerical procedure is described fro predicting the motion and structural responses of tension leg platforms(TLPs) in waves. The developed numerical approach is based on a combination of a three dimensional source distribution method and the dynamic response analysis method, in which the superstructure of TLPs is assumed flexible instead of the rigid body assumption used in tow-step analysis method. Both the hydrodynamic interactions among TLP members, such as columns and pontoons, and the structural whole structure are formulated using element-fixed coordinate systems which have the origin at the node of the each hull element and move parallel to a space-fixed coordinate system. Numerical results are compared with the experimental and numerical ones, which are obtained in the literature, concerning the motion and structural responses of a TLP in waves. The results of comparison confirmed the validity of the proposed approach.

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A Study on Computing Pit Excavation Volume by Terrain Surface Approximation (지형곡면해석에 의한 토공량 계산에 관한 연구)

  • 문두열;정범석
    • Journal of Ocean Engineering and Technology
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    • v.16 no.6
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    • pp.37-43
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    • 2002
  • The calculation of earthwork plays a major role in the plan or design phase of many civil engineering projects, such as seashore reclamation; and thus, it has become very important to improve upon its accuracy. There have been common drawbacks to earlier methods of ground profiling, such as dialing with sharp corners or the grid points of any tow straight lines. In this paper, we prepose an algorithm for finding a terrain surface using the natural boundary conditions and the both direction spline method, which interpolates the given three-dimensional data by using spline. As a result of this study, the algorithm of the proposed two methods to estimate pit excavation volume should provide a better accuracy than Spot height, Chambers, Chen, or Lin method. Also, the mathematical model mentioned offers maximum accuracy in estimating the volume of a pit excavation.

Bending Characteristics of Carbon Fabric/Polymeric Foam for Sandwich Structures (직조 탄소섬유 발포 고분자 샌드위치 구조의 굽힘특성)

  • Chang Seung Hwan;Jang Tae Seong;Choi Jin Ho;Cheon Seong Sik
    • Proceedings of the Korean Society For Composite Materials Conference
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    • 2004.10a
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    • pp.131-134
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    • 2004
  • In this paper, a representative unit volume (RUV) model was employed to simulate thermoforming process of carbon fabric-polymeric foam sandwich structures. Thermoforming simulations, which capture crimp angles and amplitude changes of carbon fabric with respect to different types of foams under the operating pressure were conducted with the help of RUV model. Changed shapes of tow structure after thermoforming were reflected in the two dimensional to determine mechanical properties of skin parts, i.e_ carbon fabric composites after thermoforming. Bending simulations with respect to different foam systems as well as different moduli of carbon fabric composites were successfully carried out by using properties obtained from two-dimensional analyses.

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