• Journal of Microbiology and Biotechnology
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• v.27 no.3
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• pp.624-632
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• 2017

Penicillium expansum causes blue mold rot, a prevalent postharvest disease of pome fruit, and is also the main producer of the patulin. However, knowledge on the molecular mechanisms involved in this pathogen-host interaction remains largely unknown. In this work, a two-dimensional gel electrophoresis-based proteomic approach was applied to probe changes in P. expansum 3.3703 cultivated in apple juice medium, which was used to mimic the in planta condition. The results showed that the pH value and reducing sugar content in the apple juice medium decreased whereas the patulin content increased with the growing of P. expansum. A total of 28 protein spots that were up-regulated in P. expansum when grown in apple juice medium were identified. Functional categorization revealed that the identified proteins were mainly related to carbohydrate metabolism, secondary metabolism, protein biosynthesis or degradation, and redox homeostasis. Remarkably, several induced proteins, including glucose dehydrogenase, galactose oxidase, and FAD-binding monooxygenase, which might be responsible for the observed medium acidification and patulin production, were also detected. Overall, the experimental results provide a comprehensive interpretation of the physiological and proteomic responses of P. expansum to the host plant environment, and future functional characterization of the identified proteins will deepen our understanding of fungi-host interactions.

• Journal of Microbiology and Biotechnology
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• v.28 no.2
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• pp.218-226
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• 2018

Nanometric Lactobacillus plantarum nF1 (nLp-nF1) is a biogenics consisting of dead L. plantarum cells pretreated with heat and a nanodispersion process. In this study, we investigated the immune-enhancing effects of nLp-nF1 in vivo and in vitro. To evaluate the immunostimulatory effects of nLp-nF1, mice immunosuppressed by cyclophosphamide (CPP) treatment were administered with nLp-nF1. As expected, CPP restricted the immune response of mice, whereas oral administration of nLp-nF1 significantly increased the total IgG in the serum, and cytokine production (interleukin-12 (IL-12) and tumor necrosis factor alpha (TNF-${\alpha}$)) in bone marrow cells. Furthermore, nLp-nF1 enhanced the production of splenic cytokines such as IL-12, TNF-${\alpha}$, and interferon gamma (IFN-${\gamma}$). In vitro, nLp-nF1 stimulated the immune response by enhancing the production of cytokines such as IL-12, TNF-${\alpha}$, and IFN-${\gamma}$. Moreover, nLp-nF1 given a food additive enhanced the immune responses when combined with various food materials in vitro. These results suggest that nLp-nF1 could be used to strengthen the immune system and recover normal immunity in people with a weak immune system, such as children, the elderly, and patients.

• The Korean Journal of Pain
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• v.31 no.3
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• pp.206-214
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• 2018

Background: Immune responses appear to be affected by anesthetics and analgesics. We investigated the effects of caudal tramadol on the postoperative immune response and pain management in pediatric patients. Methods: Sixty ASA-I pediatric patients aged 3-10 years undergoing lower abdominal surgery. Patients were randomly assigned either to a caudal bupivacaine (0.25%) group (group B), or a group that received caudal tramadol (1 mg/kg) added to the bupivacaine (0.25%) (group T). Both were diluted in a 0.9% NaCl solution to a total volume of 1ml/ kg. The systemic immune response was measured by collecting blood samples preoperatively, at the end of anesthesia, and at 24 and 72 hours postoperatively, and studied for interleukin IL-6, C-reactive proteins (CRP) cortisol levels, and leucocytes with its differential count. Postoperative pain was assessed along with sedation scales. Results: Postoperative production of IL-6 was significantly higher in group B at the end of anesthesia, than at the $24^{th}$ hour, and at the $72^{nd}$ hour in group B and group T, respectively. The immune response showed leukocytosis with increased percentages of neutrophil and monocytes, and a decreased lymphocyte response rate within both groups with no significant differences between the groups. Cortisol and CRP were significantly higher in group B. Conclusions: Adding tramadol to a caudal bupivacaine block can attenuate the pro-inflammatory cytokine response, Cortisol, and CRP in children undergoing lower abdominal surgery.

• The Journal of the Korea Contents Association
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• v.9 no.7
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• pp.294-303
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• 2009

The primary purpose of this study is to examine the effects of contents quality characteristics on user's satisfaction and continuous usage intention in UCC services. Based on relevant literature reviews, this study posits five contents quality characteristics, that is, accuracy, completeness, variety, playfulness, and timeliness as key determinants of user's satisfaction and continuous usage intention. And then we structured a research model and hypotheses about relationship between these variables. A total 315 usable survey responses of UCC service users have been employed in the analysis. The major findings from the data analyses are as follows. Firstly, four contents quality characteristics of completeness, variety, playfulness, and timeliness had a positive influence upon user's satisfaction. Secondly, four contents quality characteristics of accuracy, completeness, playfulness, and timeliness had a positive influence upon continuous usage intention. Lastly, user's satisfaction had very significantly related to continuous usage intention in UCC services. From this study, we expect to suggest practical and managerial implications to UCC service providers.

• The Journal of Industrial Distribution & Business
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• v.10 no.9
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• pp.45-52
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• 2019

Purpose - This study examined the relationship between the priorities of information, usability, mobility, reliability and empathy, which are the lower dimensions of mobile tourism app service characteristics, on the perceived value and satisfaction of tourists. Research design, data, methodology - Data collection was conducted from June 10, 2019 to June 20, 2019, and a total of 500 questionnaires were distributed to collect 352 copies. Factor analysis, reliability verification, and covariance structure analysis were carried out using SPSS 23.0 and AMOS 23.0 for 321 parts for statistical analysis. Results - First, information, mobility, reliability, and empathy have a significant effect on perceived value. However, usefulness did not appear to have a significant effect on perceived value. This analysis results can increase the reliability of the tourism app, it can be seen that the accurate tourist information services are used in the tourist app or in the place required. Second, information, usability, mobility, and empathy had a significant effect on satisfaction. However, reliability did not have a significant effect on satisfaction. Third, perceived value had a significant effect on satisfaction. Fourth, it had a significant effect on information, empathy, and intention to use. However, the usefulness, mobility, and reliability did not have a significant effect on the behavioral intention. Lastly, perceived value and satisfaction had a significant effect on behavioral intention. Conclusions - The analysis result is that the tourism app that is being used is provided with a reliable and accurate tourism information service, it can be seen that you are using the tourism app in the place or on the go. Therefore, it is relatively difficult to use the tourism app, and it is easy to understand the contents of the service and timely information. Therefore, it is possible to communicate with other users and provide customized services according to needs and desires. The study has the following limitations. This may influence the progress of the research because the respondents' responses to the mobile tourism app service are from different experiences. In addition the accuracy of providing accurate information, the usefulness of using the mobile tourism app, and the ease of communicating with other users can be understood as being relatively satisfactory.

• The Journal of the Korea Contents Association
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• v.20 no.5
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• pp.359-366
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• 2020

The purpose of this study is to explore factors inducing dental hygiene student's activeness during clinical practice. A conceptual study was conducted for dental hygiene students who participated in the clinical practice for the first semester of 2019. The focus question was, 'What factors induced activeness during clinical practice?', a total of 33 statements were drawn by analyzing the responses. Results of multidimensional scale analysis and cluster analysis, there are six factors that induce activeness 'provide information on clinical practice', 'possibility to participate in clinical practice', 'possibility of adaptation', 'attitude of educator', 'patience of educator', and 'attitude toward trainees'. Among them, the most factor inducing activeness was the 'possibility to participate in practice'. The four items inducing activeness were 'provide a training manual', 'can be observed closely,' 'practice for a sufficient period', and 'the educator's attitude is kind'. The results of this study could be used as basic data to enhance the clinical practice effect of dental hygiene students.

• Earthquakes and Structures
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• v.12 no.1
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• pp.67-77
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• 2017

In this paper, it is aimed to present a comparative study about the structural behavior of tall buildings consisting of different type of materials such as concrete, steel or timber using finite element analyses and experimental measurements on shaking table. For this purpose, two 1/60 scaled 28 and 30-stories wooden building models with $40{\times}40cm$ and $35{\times}35cm$ ground/floor area and 1.45 m-1.55 m total height are built in laboratory condition. Considering the frequency range, mode shapes, maximum displacements and relative story drifts for structural models as well as acceleration, displacement and weight limits for shaking table, to obtain the typical building response as soon as possible, balsa is selected as a material property, and additional masses are bonded to some floors. Finite element models of the building models are constituted in SAP2000 program. According to the main purposes of earthquake resistant design, three different earthquake records are used to simulate the weak, medium and strong ground motions. The displacement and acceleration time-histories are obtained for all earthquake records at the top of building models. To validate the numerical results, shaking table tests are performed. The selected earthquake records are applied to first mode (lateral) direction, and the responses are recorded by sensitive accelerometers. Comparisons between the numerical and experimental results show that shaking table tests are enough to identify the structural response of wooden buildings. Considering 20%, 10% and 5% damping rations, differences are obtained within the range 4.03-26.16%, 3.91-65.51% and 6.31-66.49% for acceleration, velocity and displacements in Model-1, respectively. Also, these differences are obtained as 0.49-31.15%, 6.03-6.66% and 16.97-66.41% for Model-2, respectively. It is thought that these differences are caused by anisotropic structural characteristic of the material due to changes in directions parallel and perpendicular to fibers, and should be minimized using the model updating procedure.

• Asian-Australasian Journal of Animal Sciences
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• v.12 no.3
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• pp.381-387
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• 1999

Two experiments were conducted to subdivide threonine (exp. 1) and glycine (exp. 2) requirements of broilers into maintenance and growth requirements. Purified diets containing five graded levels of threonine (exp. 1) and glycine (exp. 2) were fed to growing chicks to estimate threonine (exp. 1) and glycine (exp. 2) requirements for growth and maintenance. A model developed to divide threonine requirement for maintenance from that for growth yielded a requirement for growth of 8.946 mg/g weight gain and 0.341 mg/mg N gain; the maintenance requirement was 0.033 or 0.030 mg per unit of metabolic body size $(Wg^{0.75})$. The plateau of plasma threonine concentration occurred at 279.4 mg threonine intake/day. The total threonine requirement was 289.1 mg/day or 0.69% of the diet, 294.1 mg/day or 0.71% of the diet based on weight gain and nitrogen gain responses, respectively. These estimates were in close agreement with previous estimates of threonine requirements. From the relationship of weight gain to N gain, 5.46% of the retained protein consisted of threonine; the reported threonine content of chick muscle was 4.02%. The glycine requirement for maintenance could not be determined due to failure to obtain data allowing extrapolation to zero response. However, ADG increased slightly up to 0.56% glycine.

• Asian-Australasian Journal of Animal Sciences
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• v.20 no.6
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• pp.832-841
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• 2007

Rectal Temperature;Thermoregulation;Sows;Breed;The effects of season (hot vs. warm) in a tropical humid climate, parity (primiparous vs. multiparous) and breed (Creole: CR, Large White: LW) on rectal temperature (RT) were studied for a total of 222 lactations obtained in 85 sows (43 CR and 42 LW; 56 primiparous and 166 multiparous) over a 28-d lactation, between June 2002 and April 2005. Mean daily ambient temperature was higher during the hot season than during the warm season (26.0 vs. $24.1^{\circ}C$) and relative humidity was high and similar in both seasons (89% on average). At farrowing, BW was lower (172 vs. 233 kg) and backfat thickness was higher (37 vs. 21 mm) in CR than in LW sows (p<0.01). During the hot season, the reduction of average daily feed intake (ADFI) was more pronounced in LW than in CR sows (-920 vs. -480 g/d, p<0.05). Rectal temperature was higher at 1200 than at 0700hr, which coincides with the maximum and the minimum values of daily ambient temperature. The daily RT increased ($+0.9^{\circ}C$; p<0.01) between d -3 and d 7 (d 0: farrowing day), remained constant between d 7 and d 25 and decreased (p<0.01) thereafter (i.e. $-0.6^{\circ}C$ between d 25 and d 32). The average daily RT was significantly higher during the hot than during the warm season (38.9 vs. $38.6^{\circ}C$; p<0.01). It was not affected by breed, but the difference in RT between the hot and warm seasons was more pronounced in LW than in CR sows (+0.4 vs. $+0.2^{\circ}C$; p<0.05). Parity influenced the RT response; it was greater in primiparous than in multiparous sows (38.9 vs. $38.7^{\circ}C$; p<0.01). This study suggests that thermoregulatory responses to heat stress can differ between breeds and between parities.

• Asian Pacific Journal of Cancer Prevention
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• v.15 no.1
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• pp.253-256
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• 2014

Background: The standard treatment in the metastatic colorectal cancer consists of 5-FU based infusional regimens. However, with oral fluoropyrimidines, equal tumor responses may be obtained. Capecitabine causes macrocytosis of the cells by inhibition of DNA synthesis. In this context, a relationship was found between mean corpuscular volume (MCV) and response to therapy in breast cancer patients treated with Capecitabine, but whether this relationship also pertains in colorectal cancer has not been established. Materials and Methods: A total of 102 metastatic colorectal cancer patients treated with a oxaliplatin (XELOX)${\pm}$Bevacizumab combination were retrospectively evaluated. Patients were randomized into three groups. Hematological parameters (MCV, MPV, PCT, PLT, NLR) were recorded retrospectively, before treatment and after 3 cycles of chemotherapy. Results: After three cycles of therapy, 20 (19.6%) patients had progressive disease (PD), 41 (40.1%) had stable disease (SD), and 41 (40.1%) demonstrated a partial response (PR). In 62 (60.7%) treatment was with capesitabin plus XELOX therapy, and in 40 (39.2%) it was XELOX-Bevacizumab combination therapy. There was no difference among three groups before the treatment in terms of MCV, MPV, PCT, PLT, and NLR. MCV showed significant increase in chemotherapy response groups (PR and SD). In addition, a significant decrease was observed for platelet count in chemotherapy response groups. While NLR decrease was seen in only a PR group, PCT decrease was observed in all three groups. PCT and PLT values were higher in patients receiving Bevacizumab. Conclusions: PLT, PCT, MPV, and NLR values were decreased due to Capecitabine-based chemotherapy, however MCV was increased. PCT and PLT values were higher in patients who received Bevacizumab than those who did not. MCV, PLT, and NLR can be considered as important factors in predicting response to colorectal carcinoma treatment.