Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Proteome Changes in Penicillium expansum Grown in a Medium Derived from Host Plant

  • Xia, Xiaoshuang (School of Food and Biological Engineering, Jiangsu University) ;
  • Li, Huan (School of Food and Biological Engineering, Jiangsu University) ;
  • Liu, Fei (School of Food and Biological Engineering, Jiangsu University) ;
  • Zhang, Ye (School of Food and Biological Engineering, Jiangsu University) ;
  • Zhang, Qi (Key Laboratory of Detection for Mycotoxins, Ministry of Agriculture) ;
  • Wang, Yun (School of Food and Biological Engineering, Jiangsu University) ;
  • Li, Peiwu (Key Laboratory of Detection for Mycotoxins, Ministry of Agriculture)
  • Received : 2016.05.10
  • Accepted : 2016.12.19
  • Published : 2017.03.28
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Abstract

Penicillium expansum causes blue mold rot, a prevalent postharvest disease of pome fruit, and is also the main producer of the patulin. However, knowledge on the molecular mechanisms involved in this pathogen-host interaction remains largely unknown. In this work, a two-dimensional gel electrophoresis-based proteomic approach was applied to probe changes in P. expansum 3.3703 cultivated in apple juice medium, which was used to mimic the in planta condition. The results showed that the pH value and reducing sugar content in the apple juice medium decreased whereas the patulin content increased with the growing of P. expansum. A total of 28 protein spots that were up-regulated in P. expansum when grown in apple juice medium were identified. Functional categorization revealed that the identified proteins were mainly related to carbohydrate metabolism, secondary metabolism, protein biosynthesis or degradation, and redox homeostasis. Remarkably, several induced proteins, including glucose dehydrogenase, galactose oxidase, and FAD-binding monooxygenase, which might be responsible for the observed medium acidification and patulin production, were also detected. Overall, the experimental results provide a comprehensive interpretation of the physiological and proteomic responses of P. expansum to the host plant environment, and future functional characterization of the identified proteins will deepen our understanding of fungi-host interactions.

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References

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