• Title/Summary/Keyword: proteomics

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Development and Applications of Proteomics Technology (Proteomics 기술의 개발 및 응용)

  • 이지원;이은규
    • KSBB Journal
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    • v.16 no.2
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    • pp.99-106
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    • 2001
  • Proteomics research includes identification and quantitation of single protein and/or protein complex, profiling of protein expression changes in response to biological perturbations, characterization of protein functions and interactions, and elucidation the linkage between proteins and diseases. In this review paper, recent developments in the basic technologies involved in the proteomics research such as 2-dimensional PAGE and mass spectrometry are discussed. Also, the application areas of proteomics technology such as protein expression mapping and cell map proteomics are introduced with the focus on new drug development.

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Korean plant proteomics: pioneers in plant stress physiology

  • Lee, Young-Woo;Bea, Suh-Yeon;Seo, Sang-Gyu;Shim, Ie-Sung;Kim, Sun-Hyung;Kim, Sang-Gon;Kang, Kyu-Young;Kim, Sun-Tae
    • Journal of Plant Biotechnology
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    • v.38 no.2
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    • pp.151-161
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    • 2011
  • Plant proteomics is the large-scale studies of proteins, particularly on their structures and functions, governed by the physiological metabolism of plant cells. With the development of techniques and strategies in proteomics, proteomics approach is moving forward in systems biology handling sophisticated components of major signaling and biochemical pathways in plants responding to their environment. In Korea, pioneers in plant proteomics are trying to catch up with global trends in plant proteomics; these researchers are not only improving existing techniques in protein extraction but also developing new techniques in proteomics context. In the hot field of abiotic and biotic stress proteomics, 29 and 9 out of 74 papers have been published during the review period from 2005 to 2010, respectively. This present review article provides an overview on the output of Korean plant proteomers while paying special attention to both abiotic and biotic stress proteomics.

Proteomic Studies in Plants

  • Park, Ohk-Mae K.
    • BMB Reports
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    • v.37 no.1
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    • pp.133-138
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    • 2004
  • Proteomics is a leading technology for the high-throughput analysis of proteins on a genome-wide scale. With the completion of genome sequencing projects and the development of analytical methods for protein characterization, proteomics has become a major field of functional genomics. The initial objective of proteomics was the large-scale identification of all protein species in a cell or tissue. The applications are currently being extended to analyze various functional aspects of proteins such as post-translational modifications, protein-protein interactions, activities and structures. Whereas the proteomics research is quite advanced in animals and yeast as well as Escherichia coli, plant proteomics is only at the initial phase. Major studies of plant proteomics have been reported on subcellular proteomes and protein complexes (e.g. proteins in the plasma membranes, chloroplasts, mitochondria and nuclei). Here several plant proteomics studies will be presented, followed by a recent work using multidimensional protein identification technology (MudPIT).