• Title/Summary/Keyword: tolerance or resistance

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Application of genomics into rice breeding

  • Ando, Ikuo
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2017.06a
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    • pp.13-13
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    • 2017
  • By the progress of genome sequencing, infrastructures for marker-assisted breeding (MAB) of rice came to be established. Fine mapping and gene isolation have been conducted using the breeding materials derived from natural variations and artificial mutants. Such genetic analysis by the genome-wide dense markers provided us the knowledge about the many genes controlling important traits. We identified several genes or quantitative trait loci (QTL) for heading date, blast resistance, eating quality, high-temperature stress tolerance, and so on. NILs of each gene controlling heading date contribute to elongate the rice harvest period. Determination of precise gene location of blast resistance gene pi21, allowed us to overcome linkage drag, co-introduction of undesirable eating quality. We could also breed the first practical rice cultivar in Japan with a brown planthopper resistance gene bph11 in the genetic back-ground of an elite cultivar. Discovery of major and minor QTLs for good eating quality allowed us to fine-tune of eating quality according to the rice planting area or usage of rice grain. Many rice cultivars have bred efficiently by MAB for several traits, or by marker-assisted backcross breeding through chromosome segment substitution lines (CSSLs) using genetically diverse accessions. We are also systematically supporting the crop breeding of other sectors by MAB or by providing resources such as CSSLs. It is possible to pyramid many genes for important traits by using MAB, but is still difficult to improve the yielding ability. We are performing a Genomic Selection (GS) for improvement of rice biomass and grain yield. We are also trying to apply the genome editing technology for high yield rice breeding.

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Anaesthetic Tolerance of Juvenile Black Rockfish Sebastess schlegeli, Produced for Wild Stock Enhancement

  • Son, Maeng-Hyun;Park, Min-Woo;Myeong, Jeong-In;Kim, Dae-Jung;Kim, Byoung-Hak;Jo, Q-Tae;Jeon, Im-Gi
    • Ocean and Polar Research
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    • v.23 no.3
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    • pp.285-290
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    • 2001
  • The strength of juvenile black rockfish, Sebastes schlegeli, raised in different hatcheries for wild stock enhancement was evaluated in terms of resistance to an anesthetizing agent, tricaine methane-sulfonate (MS-222), and exposure to drying. The working dosage of MS-222 varied significantly with fish size and hatchery population. Smaller fish were less resistant to the chemical than larger ones. MS-222 effects also differed with fish growth history. The fish cultured in embanked populations showed stronger resistance, earlier recovery, and lower mortality, compared to those cultured in land-based tanks or collected from wild stocks. Similar results were seen in juveniles challenged to dry exposure. These results suggest that an embanked population of black rockfish is more resistant to anesthetic stress, expressed as anesthesia recovery and mortality, and that this population is healthier than others.

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Parental inheritance of heat stress tolerance during grain filling period in wheat

  • Ko, Chan Seop;Ou, Meong Kyu;Hyun, Jong Nae;Kim, Kyung Hun;Kim, Jin Baek;Hong, Min Jeong;Seo, Yong Weon
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2017.06a
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    • pp.142-142
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    • 2017
  • Wheat (Triticum asetivum L.) is one of the major grain crops worldwide. The reduced productivity ascribed by adverse environment is increasing the risk of food security. Wheat cultivars have been actively released by public side since 1960s in Korea. Each variety has been developed for superior regional adaptation, pest resistance and mostly high yield. Heat stress tolerance is one of the major parameters that threaten wheat production in Korea. Heat stress during grain filling period has been conceived as critical level and directly influences on wheat production. We evaluated 11 common wheat cultivars ("Baegjoong", "Dajung", "Goso", "Hanbaek", "Jokyoung", "Joeun", "Jopum", "Keumgang", "Olgeuru", "Sinmichal", "Uri") that were exposed to abnormally high temperature during the grain filling period. Each plant was grown well in a pot containing "Sunshine #4" soil in controlled phytotron facility set on $20^{\circ}C$ and 16 h photoperiod. At 9 day-after-anthesis (DAA9), plants were subjected to a gradual increase in temperature from $20^{\circ}C$ to $33^{\circ}C$ and maintained constantly at $33^{\circ}C$ for 5 days. After the treatment, plants were subjected to gradual decrease to normal temperature ($20^{\circ}C$) and continue to grow till harvest. Seeds were harvested from each tiller/plant. Total chlorophyll contents decrease level as well as grain parameters were measured to evaluate varietal tolerance to heat stress. We also divide each spike into five regions and evaluate grain characteristics among the regions in each spike. The obtained results allow us to classify cultivars for heat stress tolerance. The pedigree information showed that typical wheat lines provide either tolerance or susceptible trait to their off-springs, which enable breeders to develop heat stress tolerance wheat by appropriate parental choice.

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A study on the fracture behavior of Ti/Al laminates under high velocity impact (고속충격을 받는 Ti/Al 적층재의 파괴거동에 관한 연구)

  • Sohn, Se-Won;Lee, Doo-Sung;Hong, Sung-Hee
    • Proceedings of the KSME Conference
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    • 2003.04a
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    • pp.267-272
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    • 2003
  • In order to investigate the effect of face material on Ti/Al alloy laminates under high velocity impact, a ballistic testing was conducted. Ballistic resistance of these materials was measured by protection ballistic limit($V_{50}$), a statistical velocity with 50% probability penetration. Fracture behaviors and ballistic tolerance, described by penetration modes, were respectfully observed, by $V_{50}$ test and Projectile Through Plates (PTP) test at velocities greater than $V_{50}$. PTP tests were conducted with $0^{\circ}$obliquity at room temperature using 5.56mm ball projectile. $V_{50}$ tests with $0^{\circ}$obliquity were also done with projectiles that were able to achieve near or complete penetration during PTP tests. Resistance to penetration, and penetration modes which face material was Titanium alloy, were compared to those which face material was anodized Al alloy after cold-rolling.

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The Perforation Behavior of the Anodized AI Light Armor under High Velocity Impact

  • Sohn, Se-Won;Lee, Doo-Sung;Kim, Hee-Jae;Hong, Sung-Hee
    • International Journal of Precision Engineering and Manufacturing
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    • v.4 no.4
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    • pp.45-50
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    • 2003
  • In order to investigate the effect of surface treatment (Anodizing) and rolling on AI 5083-H131 alloy, under hyper velocity impact, a ballistic testing was conducted. Ballistic resistance of these materials was measured by a protection ballistic limit ($V_{50}$)' a statistical velocity with 50% probability of penetration. Perforation behavior and ballistic tolerance, described by penetration modes, were respectfully observed, by $V_{50}$ test and Projectile Through Plates (PTP) test at velocities greater than $V_{50}$. PTP tests were conducted with 0$^{\circ}$ obliquity at room temperature using 5.56mm ball projectiles. $V_{50}$ tests with 0$^{\circ}$ obliquity were also done with projectiles that were able to achieve near or complete penetration during PTP tests. Resistance to penetration, and penetration modes of Al 5052-H34 alloy were compared to those of Al 5083-H 131 alloy.

Probiotic Properties of Bifidobacteria Isolated from Feces of Infants (유아 분변에서 분리한 비피도박테리아의 프로바이오틱스 기능성 연구)

  • Kang, Chang-Ho;Kim, YongGyeong;Han, Seul Hwa;Jeong, Yulah;Park, HyeMin;Paek, Nam-Soo
    • Journal of Dairy Science and Biotechnology
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    • v.37 no.1
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    • pp.40-48
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    • 2019
  • Bifidobacteria are a prototype probiotic, which normally inhabit the intestinal tract of humans. In the present study, four species of Bifidobacterium isolated from the feces of infants were characterized. The tolerance for acid or bile salt, autoaggregation, and antibiotic resistance of the bacteria were examined. The four species were resistant to low pH, bile salts, and up to 3% bile acid. Autoaggregation rates were as high as 90%. The bacteria were consistently resistant to gentamicin, kanamycin, streptomycin, ciprofloxacin, and nalidixic acid. Due to their tolerance to environmental factors like acid and bile salts, B. longum MG723, B. breve MG729, B. bifidum MG731, and B. animalis subsp. lactis MG741 are potentially valuable as probiotics and may be useful for industrial application.

Resistance of Cucumber Grafting Rootstock Pumpkin Cultivars to Chilling and Salinity Stresses

  • Xu, Yang;Guo, Shi-rong;Li, He;Sun, Hong-zhu;Lu, Na;Shu, Sheng;Sun, Jin
    • Horticultural Science & Technology
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    • v.35 no.2
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    • pp.220-231
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    • 2017
  • Grafting using a pumpkin (Cucurbita sp.) rootstock is an effective way to improve cucumber (Cucumis sativus) resistance to a combination of chilling and salinity stresses. We evaluated the tolerance of 15 pumpkin cultivars to chilling, salinity, and combined stresses at the germination and seedling stages. Selected plant characteristics, including germination rate, germination potential, germination index, plant height, stem thickness, fresh weight, and dry weight, were analyzed. We used the unweighted pair group method with arithmetic mean for cluster analyses to determine the stress tolerance levels of the pumpkin cultivars. The 15 cultivars were divided into three clusters: tolerant, moderately tolerant, and susceptible to stress treatments. The stress tolerances of all cultivars were variable in the germination and seedling stages, and most cultivars were not tolerant to individual treatments of chilling or salinity stresses at both stages. These results suggest that identifying suitable cultivars for use as rootstock during cucumber grafting should involve the evaluation of stress tolerance during different growth stages. Additionally, cultivars tolerant to chilling stress may not be tolerant to salinity stress; therefore, the choice of pumpkin rootstock should depend on where the grafted plant will be grown. Cultivars tolerant to a combination of chilling and salinity stresses may be useful as rootstock for cucumber grafting. Our findings may serve as reference material for choosing appropriate pumpkin rootstocks for cucumber grafting.

Surface Modification of Reverse Osmosis Membrane Skin Layer by Silane Compound (Silane 화합물을 이용한 역삼투막 활성층의 표면개질)

  • Lee Yong-Taek;Shin Dong-Ho;Kim No-Won
    • Membrane Journal
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    • v.16 no.2
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    • pp.106-114
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    • 2006
  • This study is concerned with preparation of chlorine resistant (CR) thin layer composite (TFC) membranes. The novel method for making CR membranes from commercially available RO membranes is based on sol-gel condensation of trialkoxyalkylsilane derivatives. The silane coupling agents used in this study have different number of alkyl carbon chain group (methyltriethoxysilane; METES and octyltriethoxysilane; OCTES). The OCTES composite membranes have a significant tolerance to chlorine compared to commercial polyamide RO membrane or METES composite membranes. The surface properties of membranes were examined to explain a significant difference of chlorine tolerance between OCTES composite membrane and the other two membranes. In this study, we tried several surface analyses to explain difference of chlorine tolerance. The element composition results of surface analysis by EDX confirmed that both silane fixed on polyamide firmly, The surface roughness and contact angle results showed long chain alkyl group of OCTES enhancing hydrophobicity considerably than METES. The hydrophobicity plays an important role in chlorine resistance of membrane.

A Study on the Glucose and Immunoreactive Insulin Response during Oral Glucose Tolerance Test in Patients with Chronic Liver Diseases (만성(慢性) 간(肝) 질환(疾患)에서 경구적(經口的) 당부하(糖負荷) 시험시(試驗時) 혈당(血糖) 및 혈장(血漿) 인슐린의 변동(變動)에 관(關)한 연구(硏究))

  • Choe, Kang-Won;Lee, Hong-Kyu;Koh, Chang-Soon;Lee, Mun-Ho
    • The Korean Journal of Nuclear Medicine
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    • v.7 no.1
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    • pp.31-38
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    • 1973
  • The blood glucose and plasma immunoreactive insulin(IRI) levels were measured glucose tolerance test in 7 healthy subjects and 6 patients with chronic liver diseases. The glucose tolerance was impaired in 5 of the 6 patients and normal in 1. Plasma IRI responses were markedly increased and delayed in all patients, suggesting insulin resistance. Patients with more glucose intolerance showed less increase in plasma IRI than the group with less intolerance. It is suggested that some insulin antagonists may decrease the peripheral insulin sensitivity and stimulate compensatory hyperactivity of pancreatic islets. If the compensatory hyperactivity is inadequate due to genetic predisposition to diabetes mellitus or exhaustion of ${\beta}$-cells of pancreatic islsts, the glucose intolerance and overt diabetes mellitus may ensue.

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An in vitro Actinidia Bioassay to Evaluate the Resistance to Pseudomonas syringae pv. actinidiae

  • Wang, Faming;Li, Jiewei;Ye, Kaiyu;Liu, Pingping;Gong, Hongjuan;Jiang, Qiaosheng;Qi, Beibei;Mo, Quanhui
    • The Plant Pathology Journal
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    • v.35 no.4
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    • pp.372-380
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    • 2019
  • Pseudomonas syringae pv. actinidiae (Psa) is by far the most important pathogen of kiwifruit. Sustainable expansion of the kiwifruit industry requires the use of Psa-tolerant or resistant genotypes for the breeding of tolerant cultivars. However, the resistance of most existing kiwifruit cultivars and wild genotypes is poorly understood, and suitable evaluation methods of Psa resistance in Actinidia have not been established. A unique in vitro method to evaluate Psa resistance has been developed with 18 selected Actinidia genotypes. The assay involved debarking and measuring the lesions of cane pieces inoculated with the bacterium in combination with the observation of symptoms such as callus formation, sprouting of buds, and the extent to which Psa invaded xylem. Relative Psa resistance or tolerance was divided into four categories. The division results were consistent with field observations. This is the first report of an in vitro assay capable of large-scale screening of Psa-resistance in Actinidia germplasm with high accuracy and reproducibility. The assay would considerably facilitate the breeding of Psa-resistant cultivars and provide a valuable reference and inspiration for the resistance evaluation of other plants to different pathogens.