• The Korean Journal of Physiology and Pharmacology
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• v.19 no.6
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• pp.507-514
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• 2015

Nitric oxide (NO) is important in the regulation of bone remodeling, whereas high concentration of NO promotes cell death of osteoblast. However, it is not clear yet whether NO-induced autophagy is implicated in cell death or survival of osteoblast. The present study is aimed to examine the role of NO-induced autophagy in the MC3T3-E1 cells and their underlying molecular mechanism. The effect of sodium nitroprusside (SNP), an NO donor, on the cytotoxicity of the MC3T3-E1 cells was determined by MTT assay and expression of apoptosis or autophagy associated molecules was evaluated by western blot analysis. The morphological observation of autophagy and apoptosis by acridine orange stain and TUNEL assay were performed, respectively. Treatment of SNP decreased the cell viability of the MC3T3-E1 cells in dose- and time-dependent manner. SNP increased expression levels of p62, ATG7, Beclin-1 and LC3-II, as typical autophagic markers and augmented acidic autophagolysosomal vacuoles, detected by acridine orange staining. However, pretreatment with 3-methyladenine (3MA), the specific inhibitor for autophagy, decreased cell viability, whereas increased the cleavage of PARP and caspase-3 in the SNP-treated MC3T3-E1 cells. AMP-activated protein kinase (AMPK), a major autophagy regulatory kinase, was activated in SNP-treated MC3T3-E1 cells. In addition, pretreatment with compound C, an inhibitor of AMPK, decreased cell viability, whereas increased the number of apoptotic cells, cleaved PARP and caspase-3 levels compared to those of SNP-treated MC3T3-E1 cells. Taken together, it is speculated that NO-induced autophagy functions as a survival mechanism via AMPK activation against apoptosis in the MC3T3-E1 cells.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.18 no.3
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• pp.534-544
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• 2017

In order to use the sea flowering plant, Zostera marina, as a cosmetic ingredient, this study was conducted to evaluate its antioxidant effect. We confirmed the formulation stability of the emulsion containing Zostera marina extracts. We studied the antioxidant activity of the dissolved Zostera marina extracts through the DPPH (1,1-Diphenyl-2-picrylhydrazy) radical scavenging activity assay and SOD (superoxide dismutase)-like activity assay. Also, the pH, viscosity and particle dispersion of the emulsion containing Zostera marina extracts were measured using a Turbiscan LAB. The emulsions were measured at one-week intervals in a thermostat chamber ($25^{\circ}C{\pm}1^{\circ}C$, $40^{\circ}C{\pm}1^{\circ}C$) for 28 days. The extracts of Zostera marina showed a DPPH radical scavenging rate of 86.21% and SOD-like activity of 99.24% at 5.00 mg/ml and exhibited a dose-dependent increase in their antioxidant activity. We measured the stability of the pH, viscosity and emulsion particles using the Turbiscan LAB in a thermostat chamber for 28 days. The formulations to which the Zostera marina extracts were added were considered to be stable, due to their negligible physical property changes over time during storage. The results suggest that the Zostera marina extracts with 70% ethanol (v/v) could be used in cosmetics as an antioxidant for the anti-aging of skin.

• Journal of Fisheries and Marine Sciences Education
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• v.20 no.1
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• pp.127-134
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• 2008

The purpose of this study was to determine content of biogenic amines (Histamine, Cadaverine, Putrescine) in the salted mackerel, canned mackerel, mackerel pike, and freeze dried mackerel pike (Guamegi) collectively, all were purchased in the market. Salted mackerel was purchased at five markets (A, B, C, D, and F market) and stored for 10 days at $4{^{\circ}C}$. At the time of purchase, salted mackerel purchased at markets A, B and C was tested for biogenic amine, yet nothing was detected. However, salt mackerel purchased at market D was tested and the histamine concentration was 0.5 mg/100g. Mackerel from market F, exhibited content levels of 0.5 mg/100g histamine and 1.6 mg/100g cadaverine. Those make certain safety during self-life but that purchased F market gradually increased biogenic amine during storage. Prepared salted mackerel of varying levels of freshness and processing methods were analyzed for biogenic amines. Salting process was performed using functional brine salt. VBN (volatile basic nitrogen) was used to establish freshness levels of salted mackerel. Analyses of mackerel muscle determined the values of freshness to be 9.2 mg/100g (good freshness), 18.2 mg/100g (bad freshness), respectively. After 40 days of storage, the content of histamine, cadaverine, putrescine in the freshness of salted mackerel was 2.0 mg/kg, 2.4 mg/kg and 0 mg/kg, respectively. The content of histamine, cadaverine, putrescine in salted mackerel of poor freshness was 71.3 mg/kg, 22.9 mg/kg and 17.8 mg/kg, respectively. It was concluded the presence of biogenic amines during the salting process of salted mackerel significantly effected freshness of materials. The presence of biogenic amines were detected in mackerel that underwent the salting process after 1 month of storage at $4{^{\circ}C}$. The levels of biogenic amines in the brine salted mackerel were higher than those found in dry, salted mackerel; however, the freshness of fish had an insignificant effect on biogenic amines. The presence of histamine was detected in small quantities in canned mackerel and mackerel pike from three companies. Alternatively, cadaverine and putrescine were not detected. Guamegi, vacuumed packed or sealed with a rope was purchased from three markets (A, B, C seafood company). Guamegi was stored and observed for 180 days at $-20{^{\circ}C}$. Histamine was detected in small quantities in all products stored at $-20{^{\circ}C}$. Levels of histamine was dependent on the types of packaging, rope packaging yielded the highest level. However, other amines were not detected.

• Polymer(Korea)
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• v.30 no.3
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• pp.271-278
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• 2006

In order to investigate the structure and dynamics of atatic poly (vinyl alcohol) (PVA)/water system, laser light scattering experiment has been done in the semi-dilute concentration regime at $25^{\circ}C$. The scattering intensity I(q) can be analyzed with the fractal equation of $I(q){\sim}q^{-m}$ instead of Onstein-Zernike type equation. The fractal dimensionality m was found to be constant after reaching the plateau value of $m=2.6{\pm}0.3$ above C=3wt%. The time correlation function of dynamic light scattering has always two different modes such as fast mode and slow one. The cooperative diffusion of fast mode showed concentration independence contrary 4o the reptation theory's concentration dependent exponent of 3/4. The slow mode can be interpreted as the motion of large scale heterogeneities and its strong concentration dependence is apparent with a large negative exponent of -3.0. It is considered that the stereo-regular arrangement with four successive meso units of -OH plays as a key role in forming such heterogeneity.

• Proceedings of the Korea Water Resources Association Conference
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• 2008.05a
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• pp.1899-1903
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• 2008

대수층의 저유량이 풍부한 강변여과수 개발 예정지역의 충적층(지표면하 $25{\sim}35\;m$ 구간)에서 수리전도도와 종분산지수의 규모종속효과를 규명하기 위해 양수시험과 수렴흐름 추적자시험이 수행 되었다. 양수시험과 추적자시험의 규모는 2 m 와 5 m 이었으며 양수시험은 5개 공, 추적자시험은 3개 공을 이용하여 수행되었다. 양수시험은 일정한 양수율($2,500\;m^3/day$)로 수행되었으며, 양수 시작 후 경과시간에 따른 수위변화 자료를 AQTESOLV 3.5 프로그램에 입력하여 해석하였다. 시험 대수층의 수리전도도는 양수정에서 $1.745{\times}10^{-3}\;m/sec$, 양수정에서 이격거리가 2 m 구간에서는 $2.161{\times}10^{-3}\;m/sec$와 $2.270{\times}10^{-3}\;m/sec$ 이었으며, 이격거리가 5 m 구간에서는 $2.452{\times}10^{-3}\;m/sec$와 $2.591{\time}10^{-3}m/sec$로 산정되었다. 그리고, 양수정에서 회복시험 시 Theis(Recovery) 방법에 의해 해석된 수리전도도는 $1.603{\times}10^{-3}\;m/sec$이었다. 양수정에서 관측정의 이격거리(d)에 따른 수리전도도(K) 증가함수는 log K=0.0693 log d-2.671와 log K=0.0817 log d-2.655로 추정되었으며, 결정 계수는 각각 0.965와 0.979로서 매우 높게 나타났다. 따라서 양수정에서의 이격거리가 멀수록 수리전도도가 증가하는 규모종속을 확인하였으며, 또한 시험대수층의 수리전도도가 방사상으로 유사하게 분포하고 있음을 알 수 있었다. 수렴흐름 추적자시험의 양수율은 $2,500\;m^3/day$ 이었으며, 2개의 주입정에 염소이온 5 kg을 순간 주입하였다. 염소이온의 농도이력곡선을 작성하여 초기도달시간과 최고농도의 차이를 분석하였으며, 누적질량회수곡선을 통해 양수 후 경과시간에 따른 염소이온의 질량회수율을 분석하였다. 그리고, 염소이온농도 대 누적질량회수율의 이력그래프를 작성하여 누적질량회수율에 따른 염소이온농도의 증가와 감소 변화를 분석하였다. 또한, 염소이온농도의 증가/감소 구간에 대한 선형회귀분석을 수행하여 농도 증가율과 감소율의 변화를 파악하였다. 양수정에서 관측된 경과시간별 염소이온농도 자료를 CATTI 코드의 "Converging Radial Flow With Instantaneous Injection" 해석법에 적용하여 종분산지수를 추정하였다. 양수정에서 이격거리가 2 m인 경우의 종분산지수는 0.4152 m, 이격거리가 5 m인 경우의 종분산지수는 3.2665 m이었다. 따라서 양수정에서 이격거리가 멀수록 종분산지수가 증가하는 규모종속효과를 확인하였으며, 또한 이격거리에 대한 종분산지수의 비는 각각 0.21과 0.65 정도로서 증가하였다.

• Korea journal of population studies
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• v.33 no.1
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• pp.103-131
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• 2010

This study observes how the conjugal sharing of the roles such as breadwinning, housework/childcare, and leisure activities affects wives' marital satisfaction, and how the relationships among the considered variables are similar or different across age categories. Two dependent variables, the relationship satisfaction and the marital happiness, are measured and estimated as the concept of the marital satisfaction. Analysed is the Korean Longitudinal Survey of Women and Families 2008 collected by Korean Women's Development Institute. Among women in the ages of 30s-50s, full-time housewives with the breadwinning husbands feel happier with their marriage than the wives in dual career families, and those in dual career families are happier than the breadwinning women with the househusbands. Among the women in their 40s, the highest relationship satisfaction is reported by the those of the dual career families. Wives in their 30s prefer equal division of familywork in explaining the relationship satisfaction, while wives in the age of 40s and 50s show only the effect of reducing wives' portion. The co-spousal leisure activity is consistently significant in its positive relationship with the marital satisfaction across the age categories. There is a general tendency that the marital satisfaction is the highest in the youngest age bracket and the lowest in the oldest. For managing better marriages, individual efforts of both spouses as well as social support are required.

• Computational Structural Engineering
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• v.8 no.4
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• pp.87-97
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• 1995

For the same configuration of two-dimensional finite element models, 6-node element exhibits stiffer bending stiffness than 8-node element. This is true in the relation between 16-node element and 20-node element for three-dimensional model. This stiffening phenomenon comes from the elimination of several mid nodes from full-node elements. Therefore, this may be called 'relative stiffness stiffening phenomenon'. It seems that there are a couple of ways to correct the stiffening effect, however, we could find only one effective method-the method of modification of Gauss sampling points-which passes the patch test and does not alter other kinds of stiffness, such as extensional stiffness. The quantity of modification is a function of Poisson's ratios of the constituent materials. We could obtain two modification equations, one for plane stress case and the other for plane strain case. This method can be extended to 3-dimensional solid elements. Except the exact plane strain cases, most 3-dimensional plates could be modeled successfully with 16-node element modified by the equation for the plane stress case. The effectiveness of the modification method is checked by applying it to several examples with excellent improvements. In numerical examples, beams with various boundary conditions are subjected to static and time-dependent loads. Free and forced motion analyses of beams and plates are also tested. The beam and plate may be composed of isotropic multilayers as well as a single layer.

• Journal of the Korean Geotechnical Society
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• v.19 no.6
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• pp.261-271
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• 2003

A condition evaluation procedure for the pavement foundations using multi-load level Falling Weight Deflectometer(FWD) deflections is presented in this paper. A dynamic finite element program incorporating a stress-dependent material model, was used to generate the synthetic deflection database. Based on this synthetic database, the relationships between surface deflections and critical responses, such as stresses and strains in base and subgrade layers, have been established. FWD deflection data, Dynamic Cone Penetrometer(UP) data, and repeated load resilient modulus testing results used in developing this procedure were collected from the Long Term Pavement Performance (LTPP) and North Carolina Department of Transportation (NCDOT) database. Research effort focused on investigation of the effect of the FWD load level on the condition evaluation procedures. The results indicate that the proposed procedure can estimate the pavement foundation conditions. It is also found that structurally adjusted Base Damage Index (BDI) and Base Curvature Index (BCI) are good indicators for the prediction of stiffness characteristics of aggregate base and subgrade respectively. A FWD test with a load of 66.7 kN or less does not improve the accuracy of this procedure. Results from the study for the nonlinear behavior of a pavement foundations indicate that the deflection ratio obtained from multi-load level deflections can predict the type and quality of the pavement foundation materials.

• Proceedings of the Korean Geotechical Society Conference
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• 2005.03a
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• pp.328-337
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• 2005

This work reports results of our study on the dynamic responses of the buried pipelines both along the axial and the transverse directions under various boundary end conditions. We have considered three cases, i.e., the free ends, the fixed ends, and the fixed-free ends for the axial direction, and three more cases including the guided ends, the simply supported ends, and the supported-guided ends for the transverse direction. In order to investigate the effect of the boundary end conditions for the dynamic responses of the buried pipeline, we have devised a computer program to find the solutions of the formulae on the dynamic responses (displacements, axial strains, and bending strains) under the various boundary end conditions considered in this study. The dynamic behavior of the buried pipelines for the forced vibration is found to exhibit two different forms, a transient response and a steady state response, depending on the time before and after the transfer of a seismic wave on the end of the buried pipeline. The former is identified by a slight change in its behavior before the sinusoidal-shaped seismic wave travels along the whole length of the pipeline whereas the latter by the complete form of a sinusoidal wave when the wave travels throughout the pipeline. The transient response becomes insignificant as the wave speed increases. We have observed a resonance when the mode wavelength matches the wavelength of the seismic wave, where the mode number(k) of resonance for the axial direction is found to be $\overline{\omega}/{\pi}V+1/2$ for the fixed-free ends, $\overline{\omega}/{\pi}V+1$ for the free ends, and $\overline{\omega}/{\pi}V$ for the fixed ends, respectively. By adding 10 more modes to the mode number(k) of resonance, we were able to study all the dynamic responses of the buried pipeline for the axial direction. On the other hand, we have not been able to observe a resonance in the analysis for the transverse direction, because the dynamic responses are found to vanish after the seventh mode. From the results of the dynamic responses at the many points of the pipeline, we have found that the responses appeared to be dependent critically on the boundary end conditions. Such effects are found to be most prominent especially for the maximum values of the displacement and the strain and its position.

• Journal of Periodontal and Implant Science
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• v.26 no.4
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• pp.841-858
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• 1996

Epidermal growth factor(EGF) is one of polypeptide growth factors. EGF has been reported as a biological mediator which regulates activities of wound healing process including the cell proliferation, migration and metabolism. The purposes of this study is to evaluate the effects of EGF on the human periodontal ligament cells and human gingival fibroblast cells that promote regeneration of periodntal tissue. The mitogenic effects of epidermal growth factor on human periodontal ligament cells and human gingival fibroblasts were evaluated by determining the incorporation of 5-Bromo-2'-deoxy-uridine into DNA of the cells in a dose dependent manner. The prepared cells were the primary cultured gingival fibroblast and periodontal ligament cells from humans, the fourth or sixth subpassages were used in the experiments. Cells were seeded in DMEM containing 10% FBS. 1, 10, 50, 100, $200{\eta}g/ml$ and epidermal growth factor were added to the quiescent cells for 24 hours, 48 hours and 72 hours. They were labeled with $10\{mu}l/200{\mu}l$ 5-Bromo-2'-deoxy-uridine for the last 6 hours of each culture. The results of the five determinants were presented as mean and S.D.. The results were as follows : The DNA synthetic activity of human gingival fibroblasts were increased dose dependently by epidermal growth factor at 24 hours, 48 hours and 72 hours. The mitogenic effects were similar at the 24 and 48 hours of epidermal growth factor, but the DNA synthetic activity of human gingival fibroblasts generally decreased at 72 hours. The DNA synthetic activity of human periodontal ligament cells were increased dose dependently by epidermal growth factor at 24 hours but the DNA synthetic activity decreased at $200{\eta}g/ml$ of each hour. Generally the maximum mitogenic effects were observed at the 48 hours application of epidermal growth factor. The DNA synthetic activity of human periodontal ligament cells generally decreased lower at 24, 72 hours than at 48 hours the application of epidermal growth factor. In the comparison of DNA synthetic activity between human gingival fibroblasts and human periodontal ligament cells, human periodontal ligament cells had slightly higher proliferation activity than human gingival fibroblasts for a longer time at the high dosage of the epidermal growth factor. In conclusion, epidermal growth factor have important roles in the stimulation of DNA synthesis in human periodontal ligament cells and human gingival fibroblasts, and thus may be useful for clinical applications in periodontal regenerative procedures.