• Title/Summary/Keyword: time-consistency

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Optimal filter materials for protist quantification via droplet digital PCR

  • Juhee Min;Kwang Young Kim
    • ALGAE
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    • v.39 no.1
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    • pp.51-56
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    • 2024
  • The use of droplet digital polymerase chain reaction (ddPCR) has greatly improved the quantification of harmful protists, outperforming traditional methods like quantitative PCR. Notably, ddPCR provides enhanced consistency and reproducibility at it resists PCR inhibitors commonly found in environmental DNA samples. This study aimed to determine the most effective filter material for ddPCR protocols by assessing the reproducibility of species-specific gene copy numbers and filtration time across six filter types: cellulose acetate (CA), mixed cellulose ester (MCE), nylon (NY), polycarbonate (PC), polyethersulfone (PES), and polyvinylidene fluoride (PVDF). The study used two species of Chattonella marina complexes as a case study. Filtration rates were slower for NY, PC, and PVDF filters. Moreover, MCE, NY, PES, and PVDF yielded lower DNA amounts than other filters. Importantly, the CA filter exhibited the lowest variance (38-39%) and the highest determination coefficients (R2 = 0.92-0.96), indicating superior performance. These findings suggest that the CA filter is the most suitable for ddPCR quantification of marine protists, offering quick filtration and reliable reproducibility.

Point Cloud Measurement Using Improved Variance Focus Measure Operator

  • Yeni Li;Liang Hou;Yun Chen;Shaoqi Huang
    • Current Optics and Photonics
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    • v.8 no.2
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    • pp.170-182
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    • 2024
  • The dimensional accuracy and consistency of a dual oil circuit centrifugal fuel nozzle are important for fuel distribution and combustion efficiency in an engine combustion chamber. A point cloud measurement method was proposed to solve the geometric accuracy detection problem for the fuel nozzle. An improved variance focus measure operator was used to extract the depth point cloud. Compared with other traditional sharpness evaluation functions, the improved operator can generate the best evaluation curve, and has the least noise and the shortest calculation time. The experimental results of point cloud slicing measurement show that the best window size is 24 × 24 pixels. In the height measurement experiment of the standard sample block, the relative error is 2.32%, and in the fuel nozzle cone angle measurement experiment, the relative error is 2.46%, which can meet the high precision requirements of a dual oil circuit centrifugal fuel nozzle.

A Development of Model for Prospective On Dock Terminal System through Analysis of Real Case in BCTOC (BCTOC 사례분석을 통한 합리적인 온 독 시스템의 모델 개발)

  • Kim, W.K.;Do, P.L.;Kim, J.Y.;Yang, W.
    • Journal of Korean Port Research
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    • v.13 no.1
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    • pp.51-64
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    • 1999
  • This study is on the focus of the cost-effective and time-effective in the BCTOC which commenced the On Dock Termainal System in 1998 for the first time in Korea. On this process of study two categories should be considered : (1) Improvement on Business Process (2) Improvement on Information Technology. With these two accesses a new future-oriented and prospective model could be put on our hand as follows. (1) Improvement on Business Process \circled1Consistency of container In-Out Operation \circled2Simplicity of Rate appraisal \circled3Unified operation on Billing and Request (2) Improvement on Information Technology \circled1Scale up the Information Application \circled2Constructing an one-spot simultaneous Information Holding System \circled3Combination of Internet and Integrated Database System.

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Fault Detection Using Propagator for Kalman Filter and Its Application to SDINS

  • Yu, Jae-Jong;Lee, Jang-Gyu;Park, Chan-Gook
    • 제어로봇시스템학회:학술대회논문집
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    • 2003.10a
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    • pp.978-983
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    • 2003
  • In this paper, we propose a fault detection method for extended Kalman filter in decentralized filter structure. To detect a fault, a consistency between filter output and a monitoring signal is tested. State propagators are used to obtain the monitoring signal. However, the output of state propagator increases in magnitude and finally diverges as time runs. To solve such problem, two-propagator method was proposed for linear system. Two propagators are reset by Kalman filter output, alternatively, to avoid divergence. But a test statistics change abruptly at the reset instant in that method. Hence a N-step propagator method is proposed to fix up the problem. In the N-step propagator, only time propagations are performed from k-N+1 step to k step without measurement updates. A test statistics are defined by errors and its covariance between extended Kalman filter and N-step propagator. These fault detection methods are applied to integrated strapdown inertial navigation system (SDINS). By computer simulation, it is shown that the proposed methods detect a fault effectively.

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Foreground Extraction in Thermal Videos Based on Selective Histogram Bins (선택적 히스토그램 빈 기반 열화상 영상 전경 추출)

  • Yu, Gwang-Hyun;Zaheer, Muhammd Zaigham;Kim, Jin-Young;Sin, Do-Seong
    • Journal of Digital Contents Society
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    • v.19 no.4
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    • pp.757-770
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    • 2018
  • Foreground extraction is the most significant step in thermal imaging based surveillance systems. This step needs to be efficient in terms of time and memory consumption in order for the system to provide real time results but usually this efficiency reciprocates with the accurateness of the ROI detection. In this study, novel selective histogram bins based two background & foreground separation approaches for thermal videos processing have been proposed which exploit the temporal-consistency property of the thermal images in a given environment and can save over 80% memory than their simplest counterpart temporal median filtering.

A Study on Prescription Intention of Botanical Drug in Korean Medicine Doctors (한의사들의 천연물 신약 처방의사에 관한 연구)

  • Kwon, Yong Chan;Bin, Sung Oh;Koo, Jin Suk;Seo, Bu Il
    • The Korea Journal of Herbology
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    • v.30 no.5
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    • pp.7-13
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    • 2015
  • Objectives : The purpose of this study was to examine the prescription intention of Korean medicine doctors on botanical drug.Methods : The subjects in this study were 340 Korean medicine doctors in Korean medical institutions, on whom a survey was conducted from July 1 to August 31, 2014.Results : The factors that affected the prescription decision making of the Korean medicine doctors were drug superiority, level of Interest, Recognition, marketing of sales associates of pharmaceutical companies and satisfaction with information provided by those companies. When the internal consistency of the variables was measured, that was above 0.8.Conclusions : Accordingly, well-planned education and promotion efforts are required to encourage Korean medicine doctors to put more prescription intention in botanical drug. This study had some limitations : First, the subjects were selected in a manner to allow for the convenience of this researcher, and the findings might not be generalizable. Second, there was a problem with the time for the study in that there were quite fierce disputes on botanical drug at that time. Third, the majority of the subjects run their own hospitals, and the number of them was small. So it's not possible for them to represent every Korean medicine doctor.

Comparison of steady and unsteady simulation methodologies for predicting no-load speed in Francis turbines

  • Hosseinimanesh, Hossein;Devals, Christophe;Nennemann, Bernd;Guibault, Francois
    • International Journal of Fluid Machinery and Systems
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    • v.8 no.3
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    • pp.155-168
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    • 2015
  • No-load speed is an important performance factor for the safe operation of hydropower systems. In turbine design, the manufacturers must conduct several model tests to calculate the accurate value of no-load speed for the complete range of operating conditions, which are expensive and time-consuming. The present study presents steady and unsteady methods for calculating no-load speed of a Francis turbine. The steady simulations are implemented using a commercial flow solver and an iterative algorithm that relies on a smooth relation between turbine torque and speed factor. The unsteady method uses unsteady RANS simulations that have been integrated with a user subroutine to compute and return the value of runner speed, time step and friction torque. The main goal of this research is to evaluate and compare the two methods by calculating turbine dynamic parameters for three test cases consisting of high and medium head Francis turbines. Overall, the numerical results agreed well with experimental data. The unsteady method provided more accurate results in the opening angle range from 20 to 26 degrees. Nevertheless, the steady results showed more consistency than unsteady results for the three different test cases at different operating conditions.

Nonparametric estimation of hazard rates change-point (위험률의 변화점에 대한 비모수적 추정)

  • 정광모
    • The Korean Journal of Applied Statistics
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    • v.11 no.1
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    • pp.163-175
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    • 1998
  • The change of hazard rates at some unknown time point has been the interest of many statisticians. But it was restricted to the constant hazard rates which correspond to the exponential distribution. In this paper we generalize the change-point model in which any specific functional forms of hazard rates are net assumed. The assumed model includes various types of changes before and after the unknown time point. The Nelson estimator of cumulative hazard function is introduced. We estimate the change-point maximizing slope changes of Nelson estimator. Consistency and asymptotic distribution of bootstrap estimator are obtained using the martingale theory. Through a Monte Carlo study we check the performance of the proposed method. We also explain the proposed method using the Stanford Heart Transplant Data set.

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The Evaluation of Usefulness New Assistant Device to Increase Patient Convenience and Processes Efficiency of Radiographic Procedures for Merchant View (Merchant View 촬영 시 환자 편의와 업무 효율성 증대를 위한 새로운 촬영 보조기구 개발 및 유용성 평가)

  • Son, Sang-Hyuk;Kim, Sung-Kyu
    • Korean Journal of Digital Imaging in Medicine
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    • v.12 no.1
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    • pp.43-50
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    • 2010
  • The representative study of Patellofemoral Joint Merchant method can show the view for inspection of the sulcus angle, congruence angle and diagnose whether there are the vertical fractures and recognize the degree of dislocation and patella subluxation. However, anatomical correlation about the degree of knee joint curvature changes during position adjustment and distortion of the image reduces reproducibility. In order to resolve these problems, Merchant method needs to use assisting device which reduces the occurrence of repeat projection and effectively to increase unification of examination and the consistency of the image. However, there are disadvantages for patients who take other examinations. For example, they have to change the position for every examination and it might cause the patient's discomfort and increase of examination time. In this study, we newly devised commercial assisting device which improves the reproducibility of the images and reduces inconvenience of patients movement. Further research should be taken to obtain a image without patient's movement and to reduce the time of the examination than existing method.

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Wide-area Frequency-based Tripped Generator Locating Method for Interconnected Power Systems

  • Kook, Kyung-Soo;Liu, Yilu
    • Journal of Electrical Engineering and Technology
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    • v.6 no.6
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    • pp.776-785
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    • 2011
  • Since the Internet-based real-time Global Positioning System(GPS) synchronized widearea power system frequency monitoring network (FNET) was proposed in 2001, it has been monitoring the power system frequency in interconnected United States power systems and numerous interesting behaviors have been observed, including frequency excursion propagation. We address the consistency of a frequency excursion detection order of frequency disturbance recorders in FNET in relation to the same generation trip, as well as the ability to recreate by power systems dynamic simulation. We also propose a new method, as an application of FNET measurement, to locate a tripped generator using power systems dynamic simulation and wide-area frequency measurement. The simulation database of all the possible trips of generators in the interconnected power systems is created using the off-line power systems dynamic simulation. When FNET detects a sudden drop in the monitoring frequency, which is most likely due to a generation trip in power systems, the proposed algorithm locates a tripped generator by finding the best matching case of the measured frequency excursion in the simulation database in terms of the frequency drop detection order and the time of monitoring points.