Acknowledgement
This research was supported by a National Research Foundation (NRF) grant funded by the Korean government (MSIT) (NRF-2016R1A6A1A03012647, NRF-2020- R1A2C3005053, NRF-2022M3I6A1085991) to KYK.
References
- Devonshire, A. S., Honeyborne, I., Gutteridge, A., et al. 2015. Highly reproducible absolute quantification of Mycobacterium tuberculosis complex by digital PCR. Anal. Chem. 87:3706-3713. doi.org/10.1021/ac5041617
- Djurhuus, A., Port, J., Closek, C. J., et al. 2017. Evaluation of filtration and DNA extraction methods for environmental DNA biodiversity assessments across multiple trophic levels. Front. Mar. Sci. 4:314. doi.org/10.3389/fmars.2017.00314
- Harrison, J. B., Sunday, J. M. & Rogers, S. M. 2019. Predicting the fate of eDNA in the environment and implications for studying biodiversity. Proc. R. Soc. B 286:20191409. doi.org/10.1098/rspb.2019.1409
- Lee, H.-G., Kim, H. M., Min, J., et al. 2017. An advanced tool, droplet digital PCR (ddPCR), for absolute quantification of the red-tide dinoflagellate, Cochlodinium polykrikoides Margalef (Dinophyceae). Algae 32:189-197. doi.org/10.4490/algae.2017.32.9.10
- Lee, H.-G., Kim, H. M., Min, J., et al. 2020. Quantification of the paralytic shellfish poisoning dinoflagellate Alexandrium species using a digital PCR. Harmful Algae 92:101726. doi.org/10.1016/j.hal.2019.101726
- Liang, Z. & Keeley, A. 2013. Filtration recovery of extracellular DNA from environmental water samples. Environ. Sci. Technol. 47:9324-9331. doi.org/10.1021/es401342b
- Majaneva, M., Diserud, O. H., Eagle, S. H. C., Bostrom, E., Hajibabaei, M. & Ekrem, T. 2018. Environmental DNA filtration techniques affect recovered biodiversity. Sci. Rep. 8:4682. doi.org/10.1038/s41598-018-23052-8
- Min, J. & Kim, K. Y. 2022. Quantification of the ichthyotoxic raphidophyte Chattonella marina complex by applying a droplet digital PCR. Algae 37:281-291. doi. org/10.4490/algae.2022.37.11.30
- Pilliod, D. S., Goldgerg, C. S., Arkle, R. S. & Waits, L. P. 2014. Factors influencing detection of eDNA from a stream-dwelling amphibian. Mol. Ecol. Resour. 14:109-116. doi.org/10.1111/1755-0998.12159
- Pinheiro, L. B., Coleman, V. A., Hindson, C. M., et al. 2012. Evaluation of a droplet digital polymerase chain reaction format for DNA copy number quantification. Anal. Chem. 84:1003-1011. doi.org/10.1021/ac202578x
- R Core Team. 2021. R: a language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria. Available from: https://www.Rproject.org/. Accessed Sep 15, 2022.
- Reid, A. J., Carlson, A. K., Creed, I. F., et al. 2019. Emerging threats and persistent conservation challenges for freshwater biodiversity. Biol. Rev. 94:849-873. doi.org/10.1111/brv.12480
- Spens, J., Evans, A. R., Halfmaerten, D., et al. 2017. Comparison of capture and storage methods for aqueous macrobial eDNA using an optimized extraction protocol: advantage of enclosed filter. Methods Ecol. Evol. 8:635-645. doi.org/10.1111/2041-210X.12683
- Strickler, K. M., Fremier, A. K. & Goldberg, C. S. 2015. Quantifying effects of UV-B, temperature, and pH on eDNA degradation in aquatic microcosms. Biol. Conserv. 183:85-92. doi.org/10.1016/j.biocon.2014.11.038
- Van Oss, C. J., Good, R. J. & Chaudhury, M. K. 1987. Mechanism of DNA (Southern) and protein (Western) blotting on cellulose nitrate and other membranes. J. Chromatogr. A 391:53-65. doi.org/10.1016/s0021-9673(01)94304-3