• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.172-172
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• 2017

Pollen germination and its' viability in bellflower hybrid system are of great importance. The present study was conducted to investigate the several factors underlying the pollen germination of Platycodon grandiflorum and obtain the basic data for effective artificial pollination for the production of sound specifies. The pollens of Platycodon grandiflorum started germination from one hour after planting, and the germination was actively progressed as time dependent manner. For lighting conditions, the germination of pollens under the light was faster by more than twice than that without the light. Furthermore, the germination was better in the high temperature rather than in the low temperature. The germination rate was higher in the $30^{\circ}C$. For the carbon source, the germination rate was better at the concentration of 15% regardless of the kinds. In particular, the highest value was observed with glucose. The germination rate was decreased substantially as the increasing with the higher pH. The dynamic germination of pollens was observed at the pH 5. With respect to the growth regulator, the higher concentration of NAA induced the higher the germination rate. $GA_3$ showed a good germination rate in $0.05mg{\cdot}L^{-1}$. Meanwhile, for kinetin, lower concentration increased the germination rate, unlike NAA. The higher concentrations of boric acid degraded the germination rate, and the addition of boric acid of $10mg{\cdot}L^{-1}$ demonstrated higher germination rate than the addition of other growth regulators. Notably, the addition of asparaginic acid exhibited the similar results in all test sectors regardless of concentration, whereas a little higher result was observed in the high concentration sector. Taken together, the results concluded that the boric acid was considered as one of the essential minerals that played an important role on the germination of pollens.

• 한국진공학회:학술대회논문집
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• 한국진공학회 2014년도 제46회 동계 정기학술대회 초록집
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• pp.253.1-253.1
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• 2014

Plasma technology has been widely used for decontamination, differentiation, and disease treatment. Recently, studies show that plasma has effects on increasing seed germination and plant growth. In spite of increasing number of studies about plasma effects, the interaction between plasma and plants has been rarely informed. In this study, we have analyzed the effects of nonthermal atmospheric pressure plasma on seed germination and growth of coriander (Coriandum sativum), a medicinal plant. We used to Ar, air, and N2 plasma on seed as feeding gases. Plasma was discharged at 0.62 kV, 200 mA, 9.2 W. Seed germination was increased over time when treated with N2 based DBD plasma for exposure times of 30 seconds and 1 minute, everyday. After 7 days, about 80~100% of seeds were germinated in the treatment with N2 based DBD plasma, compared to control (about 40%, only gas treated seeds). In order to elucidate the mechanism of increased germination, we have analyzed characteristics of changes in plant hormones and seed surface structure by SEM.

• 한국자원식물학회지
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• 제21권3호
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• pp.171-177
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• 2008

Variations in antioxidant capacity of leaves and characteristics of seed germination among five Hibiscus hamabo populations in Korea were evaluated. While the leaves of H. hamabo from Shincheon contained highest Na concentration, those from Hado and Ohzo does lowest. MDA content and SOD activity didn't show significant difference among populations, but total protein and anthocyanin content were significantly different among five populations. The filled seed rates of five populations were ranged from 92.0 to 98.7%, and the moisture contents of seeds from Shinyang and Soando were lower than that of R. syriacus (5.18%). The fresh weights of H. hamabo from Hado and Ohzo were the highest and those of seeds from Shinyang and Soando were the lowest. Significant variations among H. hamabo populations were detected for all seed germination characters: percentage of germination, average germination time and germination rate and uniformity. SOD activities of seeds from Onpyoung and Soando were the highest of five populations, and total protein concentrations of seeds in Ohzo and Shinyang populations were higher than those of other populations. H. hamabo is considered exposed to salt stress and all characteristics of seed germinations were inferior to other species.

• Journal of Forest and Environmental Science
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• 제38권4호
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• pp.284-289
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• 2022

This study was conducted to determine the seed dormancy types of Sambucus racemosa subsp. pendula Nakai and S. williamsii Hance. Low temperature stratification (1, 2, 3, 4 months) and GA3 treatment (1,000 mg/L) were performed on seeds to determine the type of seed dormancy. After the treatment, seeds were placed on a petri dish at 25℃ under light conditions. The germination rate and mean germination time were investigated. Results showed that cold stratification was effective in breaking the dormant state of the seed in both species. In the low temperature stratification treatment, the seed germination performance was improved as the treatment period was prolonged. Gibberellin treatment was effective in breaking the dormant state of S. racemosa subsp. pendula without low temperature stratification. However, S. williamsii did not break the dormant state of the seed by gibberellin treatment without low temperature stratification treatment. In the gibberellin treatment, germination performance was improved according to the low temperature stratification treatment period. As a result of this study, the seeds of S. williamsii have both an intermediate complex and a deep complex morphophysiological dormancy (MPD). In comparison, it was found that the S. racemosa subsp. pendula had intermediate composite MPD.

• 한국식품영양학회지
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• 제7권4호
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• pp.274-283
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• 1994

To provide the efficient application scheme of buckwheat dietary fiber and basic information of seed germination, buckwheat(Fagopyrum esculentum Moench) was germinated at 10$^{\circ}C$ for 7 days and the contents and composition of the total dietary fiber(TDF), insoluble dietary fiber(IDF), soluble dietary fiber(SDF) wire examined at 24 hour intervals. The TDF content in ungerminated seeds was 24.86o on dry weight basis. It decreased for the 1st day of germination, but gradually increased for 7 days afterwords. The contents of IDF and SDF in ungerminated seeds were 22.05, 1.42% respectively. The IDF and SDF contents decreased in the initial stage of germination, but then gradually increased. The composition of the IDF and SDF in the TDF during the germination period showed different tendencies. The IDF decreased with germination time until 5 days and then increased. The SDV increased until 5 days and then decreased gradually. The TDF contents obtained by AOAC method were generally higher than those obtained by Prosky method. The TDF contents obtained by the two method, however, were very closely correlated (r=0.9966, p< 0.01) The IDF(X1) and SDF(X2) showed the significant regression equation(p<0.01) with the root length(Y). The equation was Y: -12.6681+0.5089${\times}$ 1 $\div$ 0.6022Xa and R2 was 0.968.

• 한국약용작물학회지
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• 제4권2호
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• pp.163-171
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• 1996

Lycoris의 종자발아(種子發芽) 특성(特性)을 구명(究明)하고자 온도(溫度), 채종시기(採種時期)와 파종시기(播種時期), 파종용토(播種用土)과 용토(用土)의 수분량(水分量) 및 광선(光線)의 유무(有無)가 종자발아(種子發芽)에 미치는 영향(影響)을 조사(調査)한 결과(結果)는 다음과 같다. 1. 백양꽃과 개상사화(想思花)는 90% 이상 결실(結實)하였고 종자성숙(種子成熟)은 $9{\sim}10$월 상순(上旬)으로 나타났는데 60% 이상의 발아율(發芽率)을 얻는데는 6개월이 소요되었다. 2. 발아적온(發芽適溫)은 $25^{\circ}C$로 나타났고 변온처리(變溫處理)는 $20,\;25^{\circ}C$에서 효과적(效果的)이었다. 3. 채종적기(採種適期)는 9월(月) 20일(日)경이었고 파종시기(播種時期)는 채종(採種) 즉시 파종(播種)하는 것이 가장 좋았다. 4. 파종용토(播種用土)은 사양토가 가장 좋았고 용토(用土)의 수분량(水分量)은 용토(用土) 400mg당 PF 1. 9가 가장 적당하였다. 4. 종자발아(種子發芽)는 암조건(暗條件)이 명조건(明條件)보다 좋은 것으로 나타났다.

• 미생물학회지
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• 제33권1호
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• pp.7-14
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• 1997

성장인자가 포함되지 않은 무기염 액체배지(ISP-4)에서 Streptomyces coelicolor A3(2)의 포자가 발아할 때, 성장인자의 주기적인 요구성이(양 등,1993_ 단백질과 RNA 합성과 어떤 관계를 가지고 있는지를 규명하고자 하ㅣ였다. 발아는 10시간 정도 걸렸으며 이때 성장인자의 요구성이 2시간 주기로 반복되는 것을 재입증하였다. 포자의 크기는 시간에 따라 증가하였으나, 포자수는 표준 평판계수법에서 감소하였다. 포자 집단은 생리적으로 살아있거나, 휴면 중인 포자와 죽은 포자로 구분될 수 있었다. 이러한 발아과정에서 포자를 acridine orange(AO)와 iodonitrotetrazolium chloride(INT)로 염색하여 형광현미경으로 관찰하였을 때 RNA와 단백질도 일정한 주기를 갖고 합성되었으며, 이 주기성은 성장인자의 요구 주기와 거의 일치하였다. 이로써 발아 초기에 포자 집단은 성장인자 중에서 특히 단백질 합성과 관련된 물질인 아미노산이 주기적으로 요구되고 있음을 토론하였다.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.217-217
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• 2017

Seed priming leading to improved emergences, germination speed and uniformity under unfavorable conditions without loss of longevity are the best level of techniques. We studied the possibility to obtain primed seed with reduced mean germination time (MGT) and uniformity but with simple, fast and effective methods. The experiments were carried out at $15^{\circ}C$ for 16 hours with priming treatment. To compare the effects of each treatment, we used 200mM $CaCl_2$ priming or hydro priming or no imbibition after ultrasonication or red light treatment. The light treatment was performed by irradiating 2000 lux of red light for 15, 30, 60, and 120 minutes. Addition, in order to investigate the effect of the priming solution, treated at 200mM $CaCl_2$ for 8 hours. Ultrasonication treatment was performed for 5, 10, and 20 minutes at exposures of 13.0, 17.3, and 21.6 kHz during priming. For liquid smoke (LS) treatment, seed soaked in 0%, 0.5%, 1.0%, 5.0% and 10.0% of diluted water and 200mM $CaCl_2$ solution. After each treatment, the seeds were dried to moisture content ranged 5-8% at $25^{\circ}C$ for 24 hours. The effect of seed treatment was evaluated with germination percentage (GP), MGT, germination index(GI), germination rate(GR), Germination Uniformity(GU) and heath seed percentage(HS). For several factors tested, we found that the desired germination improvement could be obtained by treating the seed with ultrasonication at 17.3 kHz for 5 minutes in water or red light exposure at 2000 lux for 120 min in water that resulted very similar to those used to 200mM $CaCl_2$ priming for 16 hrs. However, LS treatment showed no improvement in all diluted solution. Therefore, the methods applied ultrasonication and red light treatment showed high potential for fast and easy treatment avoiding pollution of salt solution waste.

• 한국산림과학회지
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• 제99권3호
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• pp.423-431
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• 2010

본 연구는 백합나무의 효율적인 기내번식법 개발을 위하여 temporary immersion 방식의 생물반응기(TIB)를 이용한 체세포배 발아에 미치는 몇 가지 요인을 구명하고자 실시하였다. 사용된 생물반응기는 간헐적으로 배지가 식물체에 접촉되도록 설계되어 배지의 침지시간을 임의로 조절하였다. 본 방식을 통한 백합나무 체세포배 발아율은 배지의 침지시간이 상대적으로 길었던 매 4시간 마다 120분씩 침지시킨 처리구와 매 4시간 마다 60분씩 침지시킨 처리구에서 높게 나타났다(각각 91.64%, 85.67%). 하지만 식물체의 과수화 비율은 침지시간이 상대적으로 짧았던 매 6시간 마다 15분씩 침지시킨 처리구와 상대적으로 길었던 매 4시간 마다 120분씩 침지시킨 처리구에서 높게 나타났다(각각 51.61%, 34.28%). 매 4시간 마다 60분씩 배지를 침지시킨 처리구에서 과수화된 식물체가 가장 적게 나타났으며, abscisic acid (ABA), superoxide dismutase (SOD) 및 catalase의 함량이 가장 적은 것으로 나타났다. 본 실험에서 생물반응기내 배지 침지 시간은 체세포배 발아시 스트레스 요소로서 작용하여 발아 및 식물체 생장의 차이를 보여주었다. 이상의 결과는 TIB방식의 생물반응기를 통해 백합나무의 효율적인 발아가 가능함을 보여주었다.

• Journal of Plant Biotechnology
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• 제48권2호
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• pp.115-122
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• 2021

Aspilia africana (Pers) C.D.Adams, a plant used for centuries in many African countries to treat diseases such as osteoporosis, malaria, tuberculosis, and diabetes, is of great pharmaceutical interest, yet there is limited scientific literature on its germination and growth. This research paper describes the effects of different commercial soils on the germination, growth, and chlorophyll content of A. africana. The germination parameters assessed included final germination percentage (FGP), mean germination time (MGT), and germination index (GI). Shoot length, leaf number, and fresh and dry weights were some of the parameters used to assess A. africana growth. The FGP was low and did not vary significantly; the MGT was 7 ~ 10 days; and the GI was significantly higher in PPS soil at 4.61 ± 0.332 days. Aspilia africana plants in HS:PPS soil showed the best overall growth, producing the highest mean leaf number (18.00 ± 1.129), longest mean shoot length (202.43 ± 13.451 mm), and highest mean fresh and dry weights (7.08 ± 1.061 g and 0.629 ± 0.112 g, respectively). The highest chlorophyll content in leaves of A. africana under HS:PPS conditions suggested a higher photosynthetic potential of plants in this soil. The best growth performance of A. africana in the HS:PPS soil could be attributed to a higher amount of certain mineral nutrients such as nitrogen, potassium, and phosphorus in the HS:PPS soil compared to the other soil categories. It is unclear why the FGP of A. africana was low and we recommend an exclusive study to investigate this further.