• Journal of Plant Biotechnology
• /
• v.44 no.2
• /
• pp.125-134
• /
• 2017

Enhanced disease susceptibility1 (EDS1) is a regulator of basal defense responses required for resistance mediated by TIR-NBS-LRR containing R proteins. We identified three transcripts of EDS1-like genes encompassing diverse/separate expression patterns, based on the transcriptome analysis by Next Generation Sequencing (NGS) of V. flexuosa inoculated with Elsinoe ampelina. These genes were designated VfEDL1 (Vitis flexuosa Enhanced Disease Susceptibility1-like1), VfEDL2 and VfEDL3, and contained 2464, 1719 and 1599 bp, with 1791, 1227 and 1599 bp open reading frames (ORFs), encoding proteins of 596, 408 and 532 amino acids, respectively. The predicted amino acid sequences of all three genes showed the L-family lipase-like domain (class 3 lipase domain), and exhibited a potential lipase catalytic triad, aspartic acid, histidine and serine in the conserved G-X-S-X-G. All three VfEDL genes were upregulated at 1 hpi against the bacterial and fungal pathogens Rizhobiumvitis and E. ampelina, respectively, except VfEDL1, which was downregulated against E. ampelina at all time points. Against E. ampelina, VfEDL2 and VfEDL3 showed downregulated expression at later time points. When evaluated against R. vitis, VfEDL1 showed downregulated expression at all time points after 1 hpi, while VfEDL3 showed upregulation up to 24 hpi. Based on the expression response, all three genes may be involved in plant resistant responses against R. vitis, and VfEDL2 and VfEDL3 show additional resistant responses against E. ampelina infection.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
• /
• v.33 no.6
• /
• pp.636-642
• /
• 2007

Background: CpG DNA plays an important role in immune cell function. This study examined whether the temporal control of toll-like receptor (TLR)9 by CpG DNA can regulate the expression of matrix metalloproteinase-9(MMP-9). Methods and materials: Macrophages were cultured in the presence of 10% FBS. For the various MMP genes analysis, RT-PCR and real-time PCR were performed. In addition, zymography assay performed for the MMP activity. The phosphorylation assay did for the ERK1/2 and NF${\kappa}B$ activation, and luciferase promoter assay was for the NF${\kappa}B$ activity. Results: CpG DNA induced the mRNA expression of MMP-2, MMP-9, and MMP-13, but not of MMP-7, MMP-8, and MMP-12, in a time-dependent manner. Especially, the mRNA expression of MMP-9 was strongly induced by CpG DNA using real-time RT-PCR. The TLR9 inhibitor, chloroquine, suppressed CpG DNA-induced MMP-9 expression and its activity. Moreover, CpG DNA induced the phosphorylation of ERK and the inhibition of ERK by U0126 suppressed CpG DNA-induced MMP-9 expression and its activity. CpG DNA stimulated $I{\kappa}B-{\alpha}$ degradation and luciferase activity. In addition, pretreatment of SN-50, the inhibitor of NF${\kappa}B$, strongly blocked the CpG DNA-induced MMP-9 expression and activity. Conclusion: These observations suggest that CpG DNA may play important roles in the activation of macrophages by regulating the production of MMP-9 via the sequential TLR9-ERK-NF${\kappa}B$ signaling pathway.

• Journal of the Korean Society of Fisheries and Ocean Technology
• /
• v.53 no.4
• /
• pp.386-395
• /
• 2017

For scientific research, a number of acoustic surveys using commercial echosounders equipped in fishing vessels were conducted throughout the world; however, few studies were performed in South Korea. Hence, this research is an preliminary study for presenting the application of a sounder from a fishing vessel. The fishing operations using a pair trawler (7 Cheonghae) was conducted in the Northwest-Western sea of Jeju Island from 20 to 23 April, 2016. Substantial impulse noises and attenuated signals were eliminated by the latest algorithms. Acoustic signals were grouped into the fish aggregations and long layer-like signals. The fish aggregations appeared between 30 and 60 m, and long layer-like signals showed the diurnal vertical migration. Energetic, morphological and positional properties of the fish aggregations and layer-like signals were described. The fish aggregations appeared mainly between sunrise and sunset; however layer-like signals tended to be presented regardless of time in consideration of the time series analysis. On the basis of the consignment sales, Scomberomorus niphonius, the target species of F/V 7 Cheonghae, was the highest catch with 4,280 kg (74.6%) and might have appeared in fish aggregations and layer forms.

• Food Science and Preservation
• /
• v.13 no.6
• /
• pp.732-739
• /
• 2006

This study was performed to investigate the changes in quality properties of extract from roasted Pleurotus eryngii by microwave-assisted extraction (MAE). MAE was carried out under different conditions including ethanol concentration, extraction time and microwave power. Total phenolics compound contents, electron donating ability (EDA) and nitrite-scavenging ability at pH 1.2 were high at 50% ethanol concentration. Superoxide dismutase (SOD)-like activity was high at 75% ethanol concentration. Soluble solid and total phenolics compound content decreased as extending extraction time up to 7 min. EDA decreased as increased extraction time. SOD-like activity and nitrite-scavenging ability were not significantly different. The highest soluble solid content, total phenolics compound content and EDA were obtained at 100 W extraction of microwave power. SOD-like activity was high at 25 W. But microwave power did not significantly affect the nitrite-scavenging ability.

• 한국HCI학회:학술대회논문집
• /
• 2008.02a
• /
• pp.141-146
• /
• 2008

As the interest about ubiquitous environment is increasing, there are many researches about the services in this environment. These services have important issues in interpreting the users' context, using many kinds of sensors, like PDA, GPS and accelerometers. Low level raw data, which sensors like accelerometers calibrates, are hard to use, and to provide real-time services preprocessing and interpreting the data into context, in real-time, is important. This paper describes a context integrate system which can integrate these sensors and also sensors which has raw data, like accelerometers and physiological sensors, and define the context interpret rule with XML. The proposing system reduces programming operations when adding a sensor to the sensor network or modifying the context interpreting rule by using XML. By using this system, we implemented a real-time data monitoring system which can describe the numeric data into graphs, and assist the user to validate the data and results of the preprocess phase, and also support the external services and applications to use the context of the user.

• Journal of Oriental Neuropsychiatry
• /
• v.24 no.3
• /
• pp.281-292
• /
• 2013

Objectives : A growing body of evidence has suggested that the dysfunction of glutamatergic systems plays a pivotal role in major depressive disorder (MDD). This study was performed to investigate the antidepressant-like effects of the ethanolic extract of Gastrodia elata Bl (GE) in mouse models and to investigate the role of ${\alpha}$-amino-3-hydroxy-5-methyl-4-isoxazole-propionic acid (AMPA) receptors in producing these antidepressant-like effects. Methods : The forced swim test (FST) and tail suspension test (TST) were used to investigate GE's behavioral effects in mice. Additional biochemical and behavioral experiments with NBQX, an AMPA receptor antagonist, were undertaken to determine whether the antidepressant-like properties of GE are involved in AMPA receptor throughput. Results : Oral administration of GE extract (1,600 mg/kg) 1h prior to testing significantly reduced the immobility times in the FST and TST. These antidepressant-like effects of GE extract were increased dose-dependently. Pre-treatment with NBQX significantly attenuated the reduction in immobility time induced by the GE extract in the FST and TST. Conclusions : The ethanolic extract of GE may exert antidepressant-like effects with involvement of AMPA receptor.

• Toxicological Research
• /
• v.32 no.4
• /
• pp.275-280
• /
• 2016

Prenatal nicotine exposure over an entire pregnancy has been associated with an increased prevalence of hyperactivity, anxiety-like behavior and depression-like behavior in mature rats. However, the effects of maternal nicotine exposure in late gestation and lactation on the psychology and behavior of adolescent rat offspring are unclear. Thus, we investigated the effect of nicotine exposure during late gestation and lactation on anxiety-like and impulsive decision-making behavior in adolescent offspring of rat. Female rats were orally exposed to nicotine which is within range of plasma level of human chronic smokers during the period of third last period of gestation and lactation. When the offspring were weaned, we observed alterations in the anxiety-like behavior and decision-making ability of adolescent rat offspring using light/dark box test and T-maze delay-based cost-benefit decision-making task. The maternal consumption of nicotine reduced both the time spent in the light compartment and the number of transitions compared to nicotine-free rats. Moreover, such nicotine exposed adolescent offspring rats showed impulsive decision making which chose the instant reward in a decision-making situation. We found that nicotine exposure during late gestation and lactation induces an increase in anxiety-like and impulsive decision-making behavior at this developmental stage. These findings suggest that maternal nicotine-exposed offspring are at an increased risk of developing anxious and impulsive behavior.

• Journal of Institute of Control, Robotics and Systems
• /
• v.12 no.2
• /
• pp.194-200
• /
• 2006

The SoC and digital technology development recently enabled the emergence of information devices and control devices because the SoC presents many advantages such like lower power consumption, greater reliability, and lower cost. However, it is nearly impossible to use the SoC without operating systems because the SoC is included with many peripherals and complex architecture. It is required to use embedded operating systems and real-time operating systems may be used as an embedded operating system. So far, real-time operating systems are widely used to implement a Real-Time system since it meets developer's requirements. However, real-time operating systems have disadvantages including a lack of standards, expensive development, and license. Embedded Linux is able to overcome their disadvantages. In this paper, the implementation of control system platform for a mobile robot using real-time Embedded Linux is described. As a control hardware system platform, XScale board is used. As the real-time Embedded Linux, RTAI is adopted which is open source and royalty free, and supports various architectures and real-time devices, such like real-time CAN and real-time COM. This paper shows the implementation of RTAI on XScale board that means the porting procedure. We also applied the control system platform to the mobile robot and compared the Real-Time serial driver with non real-time serial driver. Experimental results show that that using RTAI is useful to build real-time control system with powerful functionalities of Linux.

• Journal of Environmental Science International
• /
• v.17 no.1
• /
• pp.37-44
• /
• 2008

The electro-chemical decolorization of Rhodamine B (RhB) in water has been carried out by electro Fenton-like process. The effect of distance, material and shape of electrode, NaCl concentration, current, electric power, $H_2O_2$ and pH have been studied. The results obtained that decrease of RhB concentration of Fe(+)-Fe(-) electrode system was higher than that of other electrode system. The decrease of RhB concentration was not affected electrode distance and shape. Decolorization of electro Fenton-like reaction, which was added $H_2O_2$ onto the electrolysis using electrode was higher than electrolysis. Addition of NaCl decreased the electric consumption. The lower pH is, the faster initial reaction rate and reaction termination time observed.

• Wind and Structures
• /
• v.19 no.1
• /
• pp.89-111
• /
• 2014

The dynamics of a tornado-like vortex with touching down is investigated by using the LES turbulence model. The detailed information of the turbulent flow fields is provided and the force balances in radial and vertical directions are evaluated by using the time-averaged axisymmetric Navier-Stokes equations. The turbulence has slightly influence on the mean flow fields in the radial direction whereas it shows strong impacts in the vertical direction. In addition, the instantaneous flow fields are investigated to clarify and understand the dynamics of the vortex. An organized swirl motion is observed, which is the main source of the turbulence for the radial and tangential components, but not for the vertical component. Power spectrum analysis is conducted to quantify the organized swirl motion of the tornado-like vortex. The gust speeds are also examined and it is found to be very large near the center of vortex.