• Journal of Korean Neurosurgical Society
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• v.66 no.6
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• pp.642-651
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• 2023

Objective : Endothelial colony-forming cells (ECFCs) have been reported to play an important role in the pathogenesis of moyamoya disease (MMD). We have previously observed stagnant growth in MMD ECFCs with functional impairment of tubule formation. We aimed to verify the key regulators and related signaling pathways involved in the functional defects of MMD ECFCs. Methods : ECFCs were cultured from peripheral blood mononuclear cells of healthy volunteers (normal) and MMD patients. Low-density lipoproteins uptake, flow cytometry, high content screening, senescence-associated β-galactosidase, immunofluorescence, cell cycle, tubule formation, microarray, real-time quantitative polymerase chain reaction, small interfering RNA transfection, and western blot analyses were performed. Results : The acquisition of cells that can be cultured for a long time with the characteristics of late ECFCs was significantly lower in the MMD patients than the normal. Importantly, the MMD ECFCs showed decreased cellular proliferation with G1 cell cycle arrest and cellular senescence compared to the normal ECFCs. A pathway enrichment analysis demonstrated that the cell cycle pathway was the major enriched pathway, which is consistent with the results of the functional analysis of ECFCs. Among the genes associated with the cell cycle, cyclin-dependent kinase inhibitor 2A (CDKN2A) showed the highest expression in MMD ECFCs. Knockdown of CDKN2A in MMD ECFCs enhanced proliferation by reducing G1 cell cycle arrest and inhibiting senescence through the regulation of CDK4 and phospho retinoblastoma protein. Conclusion : Our study suggests that CDKN2A plays an important role in the growth retardation of MMD ECFCs by inducing cell cycle arrest and senescence.

• Journal of Soil and Groundwater Environment
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• v.12 no.6
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• pp.17-25
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• 2007

Convergent flow tracer test for 2 m (IW-1 well) and 5 m (IW-2 well) of test scale was conducted at the alluvial aquifer with high permeability and storativity. Pumping rate for convergent flow tracer test were $2,500m^3/day$, and the chloride tracer of 5 kg was instantaneously injected into IW-1 and IW-2 wells. Differences of first arrival time and peak concentration were analyzed by using the concentration breakthrough curves of chloride. Recovered chloride mass were analyzed by recovered cumulative mass curves. And, increment and decrement for chloride concentration were analyzed through chloride concentration versus recovered cumulative mass ratio graphs. Also, increment and decrement ratios of chloride concentration were estimated through linear regression analyses for increment and decrement intervals of chloride concentration. Longitudinal dispersivities were estimated by quot;Converging Radial Flow With Instantaneous Injectionquot; method using CATTI code. Longitudinal dispersivities estimated by CATTI code were 0.4152 m between pumping well and IW-1 well, and 3.2665 m between pumping well and IW-2 well. Longitudinal dispersivity was increased according to far distance from the pumping well. The longitudinal dispersivity according to distance were estimated as 0.21 between pumping well and IW-1 well, and 0.65 between pumping well and IW-2 well.

• Korea-Australia Rheology Journal
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• v.15 no.4
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• pp.197-208
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• 2003

Two measures of distributive mixing are examined: the standard deviation $\sigma$ and the maximum error E, among average concentrations of finite-sized samples. Curves of E versus sample size L are easily interpreted in terms of the size and intensity of the worst flaw in the mixture. E(L) is sensitive to the size of this flaw, regardless of the overall size of the mixture. The measures are used to study distributive mixing for time-periodic flows in a rectangular cavity, using the mapping method. Globally chaotic flows display a well-defined asymptotic behavior: E and $\sigma$ decrease exponentially with time, and the curves of E(L) and $\sigma$ (L) achieve a self-similar shape. This behavior is independent of the initial configuration of the fluids. Flows with large islands do not show self-similarity, and the final mixing result is strongly dependent on the initial fluid configuration.

• Proceedings of the KIEE Conference
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• 1987.07b
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• pp.1217-1220
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• 1987

We have developed a Ultra Sound Pulsed Doppler System with two-dimensional M-mode image and Spectrum analyzer. The image of the M-mode is composed of time and depth axes. The Spectrum analyzer shows the spectrum of Doppler signal which represents the velocity component of time dependent blood-flow behavior. The spectrogram using Spectrum analyzer is composed of frequency and amplitude axes. The outputs of the system are audio signals, velocity curves, velocity profiles, M-mode images and spectrogram.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.25 no.10
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• pp.1337-1345
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• 2001

Numerical investigation is made to study the effects of thermosolutal convection on the formation of macrosegregation in a Pb-Sn alloy solidification process in a two dimensional confined rectangluar mold. The basic equations are sovled using the Contrinum Model theory with the SIMPE algorithm during the solidification process. In addition, to track the liquid-solid interface with time variations, the moving boundary condition was adopted and moving irregular interface shapes were treated with the time-dependent, boundary-fitted coordinate system. As the temperature reduces from the liquidus to the solidus, the liquid concentration of Sn, the lighter constituent, increases. Then the buoyancy-driven flow due to temperature and liquid composition gradients occurs in the mushy region and forms the complicated macrosegregation maps. belated to this phenomena, effects on the macrosegregation formation depending on the cooling condition and gravity values are examined.

• 한국신재생에너지학회:학술대회논문집
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• 2008.10a
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• pp.44-46
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• 2008

Air-breathing polymer electrolyte membrane fuel cells (PEMFC) are highly promising particularly for small-power applications up to tens watts class. A distinctive feature of the air-breathing PEMFC is its simple system configuration in which axial fans operate for dual purposes, supplying both oxidant and coolant in a single manner. In the present study, a nominal 80W air-breathing PEMFC system is developed and investigated to determine the optimal operating strategy through parametric studies (i.e., reactant humidity, and fanblowing flow rate). The cell voltage distributions are examined as a function of time to evaluate the system performance under various operating conditions.

• Steel and Composite Structures
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• v.5 no.6
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• pp.421-441
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• 2005

The present work reports on a numerical simulation of a compartment fire. The fire was modeled using a simplified approach, where combustion is simulated as a volumetric heat release. Computations were performed with the commercial code CFX 5.6. Radiation was modeled with a differential approximation (P1 model), while turbulence effects upon the mean gas flow were dealt with a SST turbulence model. Simulations were carried out using a transient approach, starting at the onset of ignition. Results are provided for the temperature field time evolution, thus allowing a direct comparison with the analytical and experimental data. The high spatial resolution available for the results proved to be of great utility for a more detailed analysis of the thermal impact on the steel structure.

• Korean Journal of Air-Conditioning and Refrigeration Engineering
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• v.12 no.10
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• pp.908-916
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• 2000

In this paper, the characteristics of moisture ventilation in the lithium ion battery manufacturing dry room are studied numerically using standard $k-\varepsilon$ turbulence model. Both the steady-state and the unsteady behaviors of moisture ventilation are analyzed by considering local and uniform moisture generation. In order to evaluate the characteristics of moisture ventilation, three scales of ventilation efficiency and characteristic ventilation time are presented from the numerical results. It was shown that moisture distribution was dependent strongly on the flow field. The characteristics of moisture ventilation were improved by 20% and 40% in terms of the 1st scale of ventilation efficiency (SVE1) and the 2nd scale of ventilation efficiency (SVE2), respectively, through the modifications of design variables such as the addition of inlets, outlets and partition. A significant improvement in the characteristic ventilation time and the moisture exhaust efficiency was also made by these modifications.

• Journal of the Society of Naval Architects of Korea
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• v.41 no.4
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• pp.17-29
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• 2004

Internal waves are computed using a ghost fluid method on an unstructured grid. Discontinuities in density and dynamic pressure are captured in one cell without smearing or oscillations along a multimaterial interface. A time-accurate incompressible Navier-Stokes/Euler solver is developed based on a three-point backward difference formula for the physical time marching. Artificial compressibility is introduced with respect to pseudotime and an implicit method is used for the pseudotime iteration. To track evolution of an interface, a level set function is coupled with the governing equations. Roe's flux difference splitting method is used to calculate numerical fluxes of the coupled equations. To get higher order accuracy, dependent variables are reconstructed based on gradients which are calculated using Gauss theorem. For each edge crossing an interface, dynamic pressure is assigned for a ghost node to enforce the continuity of total pressure along the interface. Solitary internal waves are computed and the results are compared with other computational and experimental results.

• Bulletin of the Korean Chemical Society
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• v.16 no.7
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• pp.595-600
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• 1995

The kinetics of the reaction between O(3P) and C2H4 was investigated by measuring time-dependent concentrations of OH resulting from the reaction by using the LIF detection. Oxygen atoms were generated by titrating microwave discharged N2/He with NO to the chemiluminescent end point. The operating pressures in the flow reactor ranged from 5 to 15 torr and the mixtures consisted of He/O(3/P)/C2H4 in the approximate ratios from 100/1/0.1 to 100/1/1. The controlled residence time prior to the detection were estimated to be 0.8-17 ms at the reactor pressure of 7 torr. Experimentally determined profiles both in shape and magnitude were compared with the computed OH density for a specified set of experimental parameters, allowing us to arrive at a complete mechanism for the reaction of O(3P) with ethylene.