• Asian-Australasian Journal of Animal Sciences
• /
• v.27 no.8
• /
• pp.1088-1097
• /
• 2014

The study aimed to investigate the effects of gynosaponin on in vitro methanogenesis under different forage-concentrate ratios (F:C ratios). Experiment was conducted with two kinds of F:C ratios (F:C = 7:3 and F:C = 3:7) and gynosaponin addition (0 mg and 16 mg) in a $2{\times}2$ double factorial design. In the presence of gynosaponin, methane production and acetate concentration were significantly decreased, whereas concentration of propionate tended to be increased resulting in a significant reduction (p<0.05) of acetate:propionate ratio (A:P ratio), in high-forage substrate. Gynosaponin treatment increased (p<0.05) the butyrate concentration in both F:C ratios. Denaturing gradient gel electrophoresis (DGGE) analysis showed there was no apparent shift in the composition of total bacteria, protozoa and methanogens after treated by gynosaponin under both F:C ratios. The real-time polymerase chain reaction (PCR) analysis indicated that variable F:C ratios significantly affected the abundances of Fibrobacter succinogenes, Rumninococcus flavefaciens, total fungi and counts of protozoa (p<0.05), but did not affect the mcrA gene copies of methanogens and abundance of total bacteria. Counts of protozoa and abundance of F.succinogenes were decreased significantly (p<0.05), whereas mcrA gene copies of methanogens were decreased slightly (p<0.10) in high-forage substrate after treated by gynosaponin. However, gynosaponin treatment under high-concentrate level did not affect the methanogenesis, fermentation characteristics and tested microbes. Accordingly, overall results suggested that gynosaponin supplementation reduced the in vitro methanogenesis and improved rumen fermentation under highforage condition by changing the abundances of related rumen microbes.

• Bulletin of the Korean Chemical Society
• /
• v.35 no.5
• /
• pp.1279-1284
• /
• 2014

The binding of TATA-binding protein (TBP) to the TATA-box containing promoter region is aided by many other transcriptional factors including TFIIA and TFIIB. The mechanistic insight into the assembly of RNA polymerase II preinitation complex (PIC) has been gained by either directly altering a function of target protein or perturbing molecular interactions using drugs, RNAi, or aptamers. Aptamers have been found particularly useful for studying a role of a subset of PIC on transcription for their ability to inhibit specific molecular interactions. One major hurdle to the wide use of aptamers as specific inhibitors arises from the difficulty with traditional assays to validate and determine specificity, affinity, and binding epitopes for aptamers against targets. Here, using a technique called the bio-layer interferometry (BLI) designed for a label-free, real-time, and multiplexed detection of molecular interactions, we studied the assembly of a subset of PIC, TBP binding to TATA DNA, and two distinct classes of aptamers against TPB in regard to their ability to inhibit TBP binding to TFIIA or TATA DNA. Using BLI, we measured not only equilibrium binding constants ($K_D$), which were overall in close agreement with those obtained by electrophoretic mobility shift assay, but also kinetic constants of binding ($k_{on}$ and $k_{off}$), differentiating aptamers of comparable KDs by their difference in binding kinetics. The assay developed in this study can readily be adopted for high throughput validation of candidate aptamers for specificity, affinity, and epitopes, providing both equilibrium and kinetic information for aptamer interaction with targets.

• The Journal of The Korea Institute of Intelligent Transport Systems
• /
• v.14 no.5
• /
• pp.30-37
• /
• 2015

The study for enhancing the data transmission rate of the next generation wireless communication system using MIMO system operating in the frequency selective fading environment is currently actively conducted. Mixed signal from each transmitted antennas are received at antennas. The training signal with orthogonal property is needed to separate the mixed signal and enable to estimate channel and time synchronization. In this paper we introduce several training sequences used in MIMO communication system and proposed the modified WeCAN sequence with good auto-correlation property in interested area. We compared auto-correlation property of each sequence via simulation and compared the performance of sequences in doppler shift and multipath fading channel.

• Proceedings of the Korean Vacuum Society Conference
• /
• 2016.02a
• /
• pp.324-326
• /
• 2016

In this study, we have investigated indium tin zinc oxide (ITZO) as an active channel for non-volatile memory (NVM) devices. The electrical and memory characteristics of NVM devices using multi-stack gate insulator SiO2/SiOx/SiOxNy (OOxOy) with Si-rich SiOx for charge storage layer were also reported. The transmittance of ITZO films reached over 85%. Besides, ITZO-based NVM devices showed good electrical properties such as high field effect mobility of 25.8 cm2/V.s, low threshold voltage of 0.75 V, low subthreshold slope of 0.23 V/dec and high on-off current ratio of $1.25{\times}107$. The transmission Fourier Transform Infrared spectroscopy of SiOx charge storage layer with the richest silicon content showed an assignment at peaks around 2000-2300 cm-1. It indicates that many silicon phases and defect sources exist in the matrix of the SiOx films. In addition, the characteristics of NVM device showed a retention exceeding 97% of threshold voltage shift after 104 s and greater than 94% after 10 years with low operating voltage of +11 V at only 1 ms programming duration time. Therefore, the NVM fabricated by high transparent ITZO active layer and OOxOy memory stack has been applied for the flexible memory system.

• Journal of Korean Society of Transportation
• /
• v.24 no.3 s.89
• /
• pp.197-205
• /
• 2006

The study suggested a demand forecasting method which explicitly reflects transfer between various transport modes especially related light rail transit project with multi-modal transit system. The suggested method classifies several groups depending on characteristic of trips and applies different demand model for each group to explain travel pattern more realistically More specifically. the trips was classified by trips within the LRT route, trips between inside and outside of the LRT route. and through trips via the LRT route. The study also suggested a evaluation measurement of time saving due to the LRT construction, which are consistent along with the do-case and the do-nothing-case even though some mode shift could be happen after introducing the LRT.

• Journal of the Korean Society of Marine Environment & Safety
• /
• v.18 no.3
• /
• pp.193-198
• /
• 2012

Long term changes in winter time(JFM) sea surface temperature(SST) and marine ecosystem of different Korean waters during last five to six decades were illustrated. Fishing intensity with climate-ocean variability(e.g. SST) have been increasing since 1970s in all of the Korean marine waters. Winter SST around Korean waters went to colder regime in early 1980s and after the late 1980s increased gradually. After 1988/89 CRS all of the waterbody started warmer regime and well coincided with the CRS phenomena. Large predatory, small pelagic and crustacean and mollusks abundance were well coincided by the warmer SST regime after 1988/89 CRS and changed the fishery from demersal fishery to pelagic fishery. Ecosystem parameter of Mean Trophic Level(MTL) showed continuous decreasing trend since mid of 1970s which was mostly affected by the increasing of lower trophic level species. Fishing in balance(FIB) index showed increasing pattern since early 1970s to the late of 1970s and from early 1980s it was almost stable until now. Finally wasp-waist population of anchovy and Japanese sardine have a greater impact to the whole MTL since early 1970s.

• Asian-Australasian Journal of Animal Sciences
• /
• v.18 no.3
• /
• pp.427-432
• /
• 2005

The purpose of this study was performed as model study using four animals to investigate the correction between the changes in Differential Scanning Calorimetry thermogram of muscle proteins during storage and meat freshness. M. longissimus dorsi of pork was obtained immediately after slaughter and chilled/stored at either $-2^{\circ}C$ or $25^{\circ}C$ for up to 96 h for analyses. DSC thermograms were determined and compared with pH values, ATP-related compounds, K-values, volatile basic nitrogen (VBN) levels, bacterial counts and electrophoretic behavior. Changes in pH, bacterial counts, VBN and K-values were associated with increased storage temperature and time. The levels of pH values, bacterial counts, VBN and K-values of pork samples stored at $25^{\circ}C$ were higher than those of the pork samples stored at $-2^{\circ}C$. ATP concentration decreased faster in samples stored at $25^{\circ}C$. Only IMP increased in samples stored at $-2^{\circ}C$, whereas the concentration of hypoxanthine and inosine increased in samples stored at $25^{\circ}C$. One exothermic peak and two endothermic peaks appeared on the thermograms of pork stored at either temperature. Lower transition temperature of myosin, sarcoplasmic protein and actin peaks were observed. The freshness parameters of K-value, VBN and hypoxanthine showed highly negative correlations (-0.742- -0.9980) to the changes in transition temperature. Therefore, the shift temperature on DSC thermogram can be used as an indicator of the freshness parameters of meat.

• The Journal of Society for e-Business Studies
• /
• v.14 no.2
• /
• pp.41-56
• /
• 2009

The purpose of this paper is to present a statistical model that can predict the rapid shift of users' attention by analyzing the lifecycle patterns of news in a social news aggregation service. Internet news service sites have a distinct characteristic in a sense that users' attention change very quickly in a short period of time. In this research, we propose a regression model for each news category which can model the decay pattern of users' attention and the content promotion policy of a social news aggregator is proven to be a major source of the rapid growth in the popularity of news. The proposed model is expected to be useful for evaluation of the social news aggregation service provider's content promotion policy that attempts to maximize users' attention as well as the diversity of news contents.

• The Journal of Society for e-Business Studies
• /
• v.21 no.4
• /
• pp.15-28
• /
• 2016

As the industry of Mobile Internet has been gradually developed, the major device for accessing the web is predicted to shift from PC to Mobile devices in the near future. This drastic increase of demand in Mobile devices has drawn a more convenient standard of living by transcending time and space in our lives, however, it triggered various side effects such as a digital divide. This study was conducted to analyze how people utilize the contents via Mobile devices and their active attitude toward Mobile Internet by dividing individual characteristics into generations, education level and income level, and also identify whether Mobile contents utilization has an effect on the active attitude. The research results could be used for establishing a policy to narrow the gap in Mobile contents use and active attitude by individual characteristics.

• The Journal of the Institute of Internet, Broadcasting and Communication
• /
• v.17 no.1
• /
• pp.53-58
• /
• 2017

UFMC is known as the one among novel multi-carrier modulation techniques which are designed for replacing OFDM for 5G wireless communication systems. It is the generalized model of OFDM and FBMC, which combines the advantages of OFDM and FBMC and avoids their weak points. UFMC is more robust in synchronization condition like Time-frequency misalignment compared to CP-OFDM. Moreover UFMC is more proper to burst uplink transmission like M2M 5G Communications. In this paper we analyze the BER performance in various channels and speeds. The simulation result shows that the BER performance is lowered when mobile devices are moving fast and the BER performance is so sensitive for the good channel environment.