• Molecules and Cells
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• 제26권3호
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• pp.278-284
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• 2008

An open reading frame, designated GerGTII and located downstream of the polyketide synthase genes, has been identified as a chalcosyltransferase by sequence analysis in the dihydrochalcomycin biosynthetic gene cluster of Streptomyces sp. KCTC 0041BP. The deduced product of gerGTII is similar to several glycosyltransferases, authentic and putative, and it displays a consensus sequence motif that appears to be characteristic of a sub-group of these enzymes. Specific disruption of gerGTII within the S. sp. KCTC 0041BP genome by insertional in-frame deletion method, resulted complete abolishment of dihydrochalcomycin and got the 20-O-mycinosyl-dihydrochalconolide as intermediate product in dihydrochalcomycin biosynthesis which was confirmed by electron spray ionization-mass spectrometry and liquid chromatography-mass spectrometry. Dihydrochalcomycin also was recovered after complementation of gerGTII.

• BMB Reports
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• 제37권4호
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• pp.503-506
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• 2004

Glucose-1-phosphate uridylyltransferase from E. coli K12 was used to convert uridine-5'-triphosphate and glucose-1-phosphate to UDP-D-glucose. The conversion was efficient and completed within 5 minutes under the employed conditions. In addition, thymidine-5'-monophosphate kinase and acetate kinase were proven to be non-specific, converting udridine-5'-monophosphate to uridine-5'-triphosphate with 55% conversion after 6 h, which was much slower than the production of TTP under the same conditions (complete conversion within one hour). Since these two reactions could proceed under the same conditions, a one-pot synthesis of UDP-D-glucose with ATP regeneration was designed from easily available starting materials, and conversion up to 40% by HPLC peak integration was achieved given a reaction time of 4 h.

• 한국정보처리학회:학술대회논문집
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• 한국정보처리학회 2009년도 추계학술발표대회
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• pp.77-78
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• 2009

In this paper, we study a pattern-aware write cache policy using a NAND flash memory in disk-based mobile storage systems. Our work is designed to face a mix of a number of sequential accesses and fewer non-sequential ones in mobile storage systems by redirecting the latter to a NAND flash memory and the former to a disk. Experimental results show that our policy improves the overall I/O performance by reducing the overhead significantly from a non-volatile cache over a traditional one.

• 한국동물학회:학술대회논문집
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• 한국동물학회 1998년도 한국생물과학협회 학술발표대회
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• pp.218.1-218
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• 1998

No Abstract, See Full Text

• 대한용접접합학회:학술대회논문집
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• 대한용접접합학회 2006년도 춘계 학술대회 개요집
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• pp.100-102
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• 2006

• Journal of Microbiology and Biotechnology
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• 제28권2호
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• pp.298-304
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• 2018

The single-vessel multienzyme UDP-${\alpha}$-$\text\tiny{D}$-glucose recycling system was coupled with a forward glucosylation reaction to produce novel glucose moiety-conjugated derivatives of different tetracycline antibiotic analogs. Among five tetracycline analogs used for the reaction, four molecules (chlorotetracycline, doxytetracycline, meclotetracycline, and minotetracycline) were accepted by a glycosyltransferase enzyme, YjiC, from Bacillus licheniformis to produce glucoside derivatives. However, the enzyme was unable to conjugate sugar units to rolitetracycline. All glucosides of tetracycline derivatives were characterized by ultraviolet absorbance maxima, ultra-pressure liquid chromatography coupled with photodiode array, and high-resolution quadruple time-of-flight electrospray mass spectrometry analyses. These synthesized glucosides are novel tetracycline derivatives.

• 한국멀티미디어학회논문지
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• 제17권11호
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• pp.1263-1269
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• 2014

As rapid development of new hardware control interface technology, new concepts have been being proposed and emerged. In this paper, a new approach based on leap motion system is proposed. While we employ a position information from sensor, the hand gesture recognition is suggested with the pre-defined patterns. To do this, we design a recognition algorithm with hand gesture and finger patterns. We apply the proposed scheme to 3-dimensional avatar controling and editing software tool for making animation in the cyber space as a representative application. This proposed algorithm can be used to control computer systems in medical treatment, game, education and other various areas.

• 한국산학기술학회:학술대회논문집
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• 한국산학기술학회 2011년도 춘계학술논문집 1부
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• pp.126-129
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• 2011

본 연구에서는 경제성장과 발전이라는 목표로 인해 오로지 성장만을 위해 달려온 현대시대가 직면해 있는 문제점들에 대한 새로운 방향전환으로 진행되고 있는 생태 하천 복원사업의 장점 및 문제점 등을 하천 복원 사례의 비교분석을 통해 파악하고자 하였다. 이를 위하여 생태하천 복원사업이 이루어진 양재천, 학의천, 홍재천의 답사를 통하여 하천자연도 평가점수표를 작성하였으며, 문헌조사를 통하여 생태하천 복원결과 및 효과를 비교하였다. 이에 따라 생태하천 복원사업에서 반드시 고려해야할 사항을 정리하고 앞으로의 발전방향을 제시하였다.

• Molecules and Cells
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• 제26권4호
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• pp.362-367
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• 2008

We identified a 1,134-bp putative type III polyketide synthase from the sequence analysis of Streptomyces peucetius ATCC 27952, named Sp-RppA, which is characterized as 1,3,6,8-tetrahydroxynaphthalene synthase and shares 33% identity with SCO1206 from S. coelicolor A3(2) and 32% identity with RppA from S. griseus. The 1,3,6,8-tetrahydroxynaphthalene synthase is known to catalyze the sequential decarboxylative condensation, intramolecular cyclization, and aromatization of an oligoketide derived from five units of malonyl-CoA to give 1,3,6,8-tetrahydroxynaphthalene, which spontaneously oxidizes to form 2,5,7-trihydroxy-1,4-naphthoquinone (flaviolin). In this study, we report the in vivo expression and in vitro synthesis of flaviolin from purified gene product (Sp-RppA).

• Neonatal Medicine
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• 제16권1호
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• pp.76-80
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• 2009

저자들은 자궁 내 발육 지연으로 입원한 신새아가 요도하열, 잠복고환, 폐동맥판 협착이 동반되어 시행한 염색체 검사에서 불균형 전도로부터 재조합된 염색체이상의 결과로 8번 염색체 단완 결실과 장완 중복을 보인 1례를 경험하였기에 문헌 고찰과 함께 보고하는 바이다.