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Antioxidant and Anti-inflammatory Properties of Two Extracts from Lycium ruthenicum Murray Fruit (흑구기자 열매의 생리활성 평가 연구)

  • Zou, Jie;Lee, Ji-An
    • Journal of Convergence for Information Technology
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    • v.11 no.5
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    • pp.190-198
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    • 2021
  • The aim of the study was to evaluate the physiological activity of Lycium ruthenicum to investigate their potential as a raw material that can be used in the cosmetic industry. L. ruthenicum fruit extracts were obtained using 70% methanol(LRM) and hot-water(LRW). The DPPH and ABTS radical scavenging abilities were higher in the LRM extract than in the LRW extract. The FRAP value of LRM was about 1.3-fold greater than that of LRW. The polyphenol contents of LRM and LRW were 31.883±1.395 mg/g and 27.748±2.741 mg/g respectively. LRM inhibited the generation of NO in LPS-stimulated RAW264.7 cells. LRM also attenuated the expression of COX-2, PGE2, IL-6, and TNF-α induced by LPS. These results suggests that L. ruthenicum fruits could be use as a source of natural antioxidants and anti-inflammatory agent in cosmetic products.

The Qualitative Analysis on Experiences of Caregiving Middle-aged Woman's Biological Parents (중년기 여성의 친부모 부양경험에 관한 질적 연구)

  • Park, Jong-Hwan;Shin, Seung-Ok
    • The Journal of the Korea Contents Association
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    • v.18 no.12
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    • pp.611-623
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    • 2018
  • Ten middle-aged women who support their biological parents have been interviewed to investigate their experiences of caregiving their parents. As a qualitative research method, data were collected through in-depth interviews and analyzed by Colaizzi's phenomenological method. Consequently 244 meaningful sentences were selected from the collected interview data and then were sorted into 67 meaningful sentences and, consequently into 9 sub-themes. Finally four high-level themes are wrapped through the summarizing process. The first theme is motivation of caregiving. Inevitable cases are most common for caregiving their biological parents. And, general responsibilities to their parents are also deduced as a motivation of caregiving. The second theme is a psychological phenomenon of middle-aged women while they provide consistent caregiving their biological parents even though they have some troubles with their parents. The third theme is meaning of caregiving. For some women, caregiving their biological parents is their own happiness and gratitude such as lifelong gifts. The final theme is influence of caregiving. Further understanding their parents, and more distinct prospecting their future lives are unexpected fruits from the caregiving their biological parents.

Developmental inhibition of Drosophila suzukii by ionizing radiation

  • KIM, Junheon;KIM, Jeongmin;LEE, Yeon Jeong;PARK, Chung Gyoo
    • Entomological Research
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    • v.48 no.5
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    • pp.331-338
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    • 2018
  • Spotted wing drosophila (SWD) has emerged as a major invasive insect pest of small berry fruits in the Americas and Europe since the late 2000s. Thus, phytosanitary treatment of commodities for export is imperative to prevent the movement of viable SWD to newer areas. In the present study, all developmental stages of SWD were irradiated with different doses of gamma and electron beam radiation to assess developmental inhibition to identify potential quarantine doses of the radiations. Ionizing radiation induced developmental inhibition of all stages of SWD. The effective doses for 99% inhibition ($ED_{99}$) of hatching, pupariation, and adult emergence from irradiated eggs for gamma radiation were 882, 395 and 39 Gy, respectively, compared with 2849, 687, and 41 Gy, respectively, for electron beam radiation. The $ED_{99}$ for inhibition of pupariation and adult emergence in irradiated larvae were 703 and 47 Gy, respectively, for gamma radiation, and 619 and 33 Gy, respectively, for electron beam radiation. Pupal irradiation did not completely inhibit adult emergence, even at 300 Gy. However, irradiation with ${\geq}100Gy$ of puparia induced adult sterility, with no egg production at all. The $ED_{99}$ for inhibition of $F_1$ egg hatchability from adults irradiated with gamma radiation and electron beam radiation was estimated to be 424 and 125 Gy, respectively. The results of the present study suggest that gamma radiation and electron beam radiation are alternatives for phytosanitary treatment. Irradiation with 100 Gy could be suggested as a potential dose for egg, larval, and pupal quarantine treatment of SWD.

Fruit Consumption by Fruit Type and Its Relation to Metabolic Factors in Korean Adults - 2016 Korea National Health and Nutrition Survey - (한국 성인에서 과일 종류별 섭취 및 대사적 지표와의 관련성 - 2016 국민건강영양조사 자료를 활용하여 -)

  • Bae, Yun-Jung;Lee, Kyung-Haeng;Yu, Kwangwon
    • The Korean Journal of Food And Nutrition
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    • v.33 no.6
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    • pp.721-731
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    • 2020
  • This study used a food-frequency questionnaire to estimate fruit consumption by sex, age, and type of fruit to analyze the association between fruit type and metabolic factors. Using food-frequency-questionnaire data from 3,091 adults aged 19~64 years (1,184 men and 1,907 women) in the 2016 Korea National Health and Nutrition Examination Survey, this study analyzed weekly fruit consumption and the consumption frequency of strawberry, oriental melon, watermelon, peach, grape, apple, pear, persimmon/dried persimmon, tangerine, banana, orange, and kiwi by sex and age groups. Apple was the most-consumed fruit (459.45 g/wk), followed by pear (165.80 g/wk), watermelon (124.80 g/wk), and banana (115.32 g/wk). After adjustment for confounding factors, ED: Please give examples. in the women who ate more than the median consumption of apple, the prevalence of hyperglycemia was 27.4% (OR: 0.726, 95% CI: 0.539~0.979) (p=0.0362), and of high triglycerides, 25.7% (OR: 0.743, 95% CI: 0.564~0.978) (p=0.0340). These rates were lower than those of women who ate less than the median consumption of apple. The results of this study suggest that apple consumption helps improve metabolic indicators in Korean adults.

Oxidative Stability of Vacuum-Packed Chicken Wings Marinated with Fruit Juices during Frozen Storage

  • Rupasinghe, Rashmi A.;Alahakoon, Amali U.;Alakolanga, Achala W.;Jayasena, Dinesh D.;Jo, Cheorun
    • Food Science of Animal Resources
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    • v.42 no.1
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    • pp.61-72
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    • 2022
  • Antioxidants present in fruits and vegetables have a potential to reduce disease risk, and increase the shelf life of food products by reducing lipid oxidation. The effect of marination with antioxidants-rich fruit juices on quality characteristics of vacuum-packed chicken wings were examined during frozen storage. Chicken wings were mixed separately with marinades containing pineapple juice, June plum juice, and mango juice and kept for 12 h and 24 h. Three best marination conditions were selected based on a sensory evaluation. Antioxidant activity and total phenolic content of fruit juices, and marinade uptake, and marinade loss of marinated chicken wings were determined. In addition, vacuum packed marinated chicken wings were tested for pH, water holding capacity (WHC), 2-thiobarbituric acid reactive substances (TBARS) value and antioxidant activity over a 4-wk frozen storage. The best sensory properties were reported from chicken wings marinated with pineapple juice for 24 h, mango juice for 24 h, and June plum juice for 12 h (p<0.05) compared to other marinade-time combinations. Mango juice showed the highest antioxidant activity (92.2%) and total phenolic content (38.45 ㎍/mL; p<0.05) compared to other fruit juices. The pH and WHC of vacuumpacked chicken wings were slightly decreased over the frozen storage (p<0.05). Moreover, chicken wings marinated with mango juice had the lowest TBARS values and the highest 2,2-diphenyl-1-picryl-hydrazyl-hydrate free radical scavenging activity. In conclusion, mango juice was selected among tested as the most effective marinade for enhancing the oxidative stability of lipid while maintaining the other meat quality traits of vacuum-packed chicken wings.

An External and Micromorphological Identification for Pharbitidis Semen and its Congeneric Species (외부 및 미세형태 비교를 통한 견우자(牽牛子) 기원종 및 동속이종(同屬異種) 감별)

  • Song, Jun-Ho;Yang, Sungyu;Choi, Goya;Moon, Byeong Cheol
    • The Korea Journal of Herbology
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    • v.33 no.4
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    • pp.43-51
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    • 2018
  • Objectives : Pharbitidis Semen, the seeds of Ipomoea nil (L.) Roth or I. purpurea (L.) Roth, is well-known traditional herbal medicine in Korea. But it is often marketed as a different seed or mixtures of its closely related species. Thus, the present study aims to provide external and micromorphological characters and identification key by using stereoscope (ST) and scanning electron microscope (SEM) for discriminating authentic of Pharbitidis Semen. Methods : A discrimination on external morphological characteristics of sepals, fruits, seeds, and hilum, testa cell micromorphology in the original plants and its congeneric species was carried out using digital calipers, ST, and SEM. Results : Number of valves (degree of apex of each valve), number of seeds per locule, hairy in capsules and size, luster, density of hairy, hilum shape in seeds and shape of cell, anticlinal, periclinal wall in testa may have high discriminative value. The seeds of Ipomoea nil as an original plant of Pharbitidis Semen were distinguished from other species by the relative larger in size, ovoid-trigonous in shape, mostly flabellate or triangular to trapezoid in outline (c.s.), dull, and puberulent in surface and thicken anticlinal wall. Conclusions : On the basis of the results, an identification key of Pharbitidis Semen and closely related species is provided. Our observations suggest that the combination of morphological characters and other studied results could be helpful in the successfully identified authentic herbal medicines. Moreover, micromorphological characters using SEM could be useful for discriminating authentic medicines.

A review of the immunomodulatory activities of polysaccharides isolated from Panax species

  • Hu, Yeye;He, Yang;Niu, Zhiqiang;Shen, Ting;Zhang, Ji;Wang, Xinfeng;Hu, Weicheng;Cho, Jae Youl
    • Journal of Ginseng Research
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    • v.46 no.1
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    • pp.23-32
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    • 2022
  • Panax polysaccharides are biopolymers that are isolated and purified from the roots, stems, leaves, flowers, and fruits of Panax L. plants, which have attracted considerable attention because of their immunomodulatory activities. In this paper, the composition and structural characteristics of purified polysaccharides are reviewed. Moreover, the immunomodulatory activities of polysaccharides are described both in vivo and in vitro. In vitro, Panax polysaccharides exert immunomodulatory functions mainly by activating macrophages, dendritic cells, and the complement system. In vivo, Panax polysaccharides can increase the immune organ indices and stimulate lymphocytes. In addition, this paper also discusses the membrane receptors and various signalling pathways of immune cells. Panax polysaccharides have many beneficial therapeutic effects, including enhancing or activating the immune response, and may be helpful in treating cancer, sepsis, osteoporosis, and other conditions. Panax polysaccharides have the potential for use in the development of novel therapeutic agents or adjuvants with beneficial immunomodulatory properties.

Evaluation of Antioxidant and Anticancer Activity of Guarana and Graviola in Human Colon Cancer (과라나와 그라비올라가 인체 대장암에 미치는 항산화 및 항암효과에 대한 연구)

  • Lee, Myeong-Seon
    • Journal of Korea Entertainment Industry Association
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    • v.13 no.1
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    • pp.217-223
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    • 2019
  • Colon cancer is the most common form of cancer diagnosis in worldwide. There are growing interests in the health benefits associated with consumption of fruits and vegetables, especially for the prevention of cancer, cardiovascular or other chronic diseases. The objective of the present study was to investigate the antioxidant and anticancer activities of natural product, guarana(GR) and graviola(GV) in human colon carcinoma HCT-116 cells. MTT assay, flow cytometry analysis were employed to investigate the anticancer mechanism and DPPH assay was determined to the antioxidant activity to scavenge free radicals in extract of these. All two extracts showed significantly antioxidant activity at 50mg/ml of concentration. GR and GV reduced HCT-116 cell proliferation in a dose dependent manner. Specially GR treatment(96.65±3.71) also significantly increased the sub-G1 population more than GV(79.58±2.87) treatment in HCT-116 at the concentration of 10mg/ml, as shown by flow cytometry assay. Statistical analyses revealed GR and GV exhibited significantly high (P < 0.05) cytotoxicity in HCT-116. These findings indicate that GN and GV may serve as novel therapeutic agents for colon cancer treatment and future leads for drug development.

Potentiality of Beneficial Microbe Bacillus siamensis GP-P8 for the Suppression of Anthracnose Pathogens and Pepper Plant Growth Promotion

  • Ji Min Woo;Hyun Seung Kim;In Kyu Lee;Eun Jeong Byeon;Won Jun Chang;Youn Su Lee
    • The Plant Pathology Journal
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    • v.40 no.4
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    • pp.346-357
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    • 2024
  • This study was carried out to screen the antifungal activity against Colletotrichum acutatum, Colletotrichum dematium, and Colletotrichum coccodes. Bacterial isolate GP-P8 from pepper soil was found to be effective against the tested pathogens with an average inhibition rate of 70.7% in in vitro dual culture assays. 16S rRNA gene sequencing analysis result showed that the effective bacterial isolate as Bacillus siamensis. Biochemical characterization of GP-P8 was also performed. According to the results, protease and cellulose, siderophore production, phosphate solubilization, starch hydrolysis, and indole-3-acetic acid production were shown by the GP-P8. Using specific primers, genes involved in the production of antibiotics, such as iturin, fengycin, difficidin, bacilysin, bacillibactin, surfactin, macrolactin, and bacillaene were also detected in B. siamensis GP-P8. Identification and analysis of volatile organic compounds through solid phase microextraction/gas chromatography-mass spectrometry (SPME/GC-MS) revealed that acetoin and 2,3-butanediol were produced by isolate GP-P8. In vivo tests showed that GP-P8 significantly reduced the anthracnose disease caused by C. acutatum, and enhanced the growth of pepper plant. Reverse transcription polymerase chain reaction analysis of pepper fruits revealed that GP-P8 treated pepper plants showed increased expression of immune genes such as CaPR1, CaPR4, CaNPR1, CaMAPK4, CaJA2, and CaERF53. These results strongly suggest that GP-P8 could be a promising biocontrol agent against pepper anthracnose disease and possibly a pepper plant growth-promoting agent.

A Strip Sensor Based on PbO2/Carbon Paste Electrode to Determine Sweetener Contents in Fruits (이산화납/탄소 반죽 전극을 이용한 과당 농도 측정 스트립센서)

  • Lee, Jae Seon;Cho, Joo Young;Heo, Min;Lim, Woo-Jin;Lee, Sang Eun;Nam, Hakhyun;Cha, Geun Sig;Shin, Jae Ho
    • Journal of the Korean Electrochemical Society
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    • v.17 no.2
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    • pp.130-137
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    • 2014
  • A strip sensor based on $PbO_2$/carbon paste electrode was prepared by a screen-printing method, and employed to electrochemically determine the concentration of fruit sweeteners(i.e. glucose, sucrose, and fructose). The $PbO_2$/carbon paste electrode could monitor electrocatalytic oxidation of organic compounds such as carbohydrates, and measure the levels of natural sweeteners without enzyme. Severe interference from ascorbic acid was effectively reduced by modifying the electrode surface with a Nafion membrane. The response level of the Nafion/$PbO_2$/carbon paste electrode increased in the order of fructose, sucrose, and glucose, which corresponds to the order of sweetness perceived by humans.