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An Analysis of Morphological Variation in Abies koreana Wilson and A. nephrolepis (Traut.) Maxim. of Korea (Pinaceae) and Their Phylogenetic Problems (한국산(韓國産) 분비나무와 구상나무의 형질분석(形質分析)과 종간유연관계(種間類緣關係))

  • Chang, Chin-Sung;Jeon, Jeong Ill;Hyun, Jung Oh
    • Journal of Korean Society of Forest Science
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    • v.86 no.3
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    • pp.378-390
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    • 1997
  • Ten total populations of Korean fir (Abies koreana Wilson) and Manshurian fir [A. nephrolepis (Traut.) Maxim.] were sampled from south Korea to investigate patterns of intraspecific variation in these species and to evaluate a recognition of the two species. Principal components analysis and cluster analysis were performed both on seed-cone data and on needle morphology data. The characters that contributed most to the separation between A. koreana and A. nephrolepis along three principal components axis were leaf width, length of seed, width of seed wing, length of seed wing, cone width, width of scale, and length of bract tip, but these characters were not diagnostic because of overlap in reality. Therefore, all these characters were not reliable in distinguishing these two taxa including bract position (exerted and recurved vs. exerted and straight). The individuals of A. koreana from Mt. Chi-ri appeared quite unique probably on account of its larger cone size and longer scale tip, while those from Mt. Hal-la of A. koreana were generally distinct from others in terms of their larger seed and seed wing and longer scale width. The Mt. Duk-yu specimens of A. korecana appeared somewhat smaller but more data were needed due to the small sampling size. Generally, the gradual clinal geographic trends made evident by the position of resin ducts in leaves of A. koreana can be detected. The southern populations, Mt. Hal-la (an insular population) were generally distinct from the northern populations (Mt. Chi-ri, Mt. Ga-ya and Mt. Duk-yu) in terms of their position of resin duct (medial, within mesophyll vs marginal, close to epidermis : 100% vs 75 or 50%). Although no sharp boundary separating these two species could be detected based on cone and needle morphology, the observed clinal pattern was distinct in northern populations of A. koreana and southern population of A. nephrnlepis. In a preceding study of the flavonoids variation of 20 species in eastern Asia, flavanone (5-deoxyflavanone) was found to be characteristic of A. faxoniana Rehder et Wilson, A. georgei Orr of China and A. koreana of Korea. A. faxoniana, which is assumed to be primitive species, has position of resin duct relative to both the medial and the marginal, while A. georgei and A. koreana are identified by marginal position of resin duct. With respect of foliar flavonoids chemistry, A. koreana was distinct from A. nephrolepis : the southmost samples (Mt. Hal-la and Mt. Chi-ri) contained additional flavonoids derivatives (mainly flavanone) that were not found in the northmost samples of A. nephrolepis except a few individuals from Mts. Seo-rak and Tae-bak populations of Kwang-won province. The presence of A. koreana type flavonoids in two Chinese species suggested that position of resin duct may be a phyletic character. Abies koreana including two Chinese taxa, exhibited the most elaborate and specialized flavonoids profile within the Abies in eastern Asia. Contrary to our initial expectations, the apparent intermediates between A. nephrolepis and A. koreana in Duk-yu and Ga-ya mountains were found. The pattern of variation on position of resin duct and flavonoids chemistry in these populations of A. kareana suggested that genetic interchange or natural hybridization had occurred between these two species. The evidence needed to resolve the status of this taxon is still inconclusive in our opinion until intermediate individuals from Mts. Duk-yu and Ga-ya show indication of hybridization between the two species.

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A Retrospective Study of 94 Hypercalcemic Dogs(2002-2004) (94 마리 고칼슘혈증 개들에 대한 회고연구(2002-2004))

  • Cho, Tae-Hyung;Kang, Byeong-Teck;Park, Chul;Jung, Dong-In;Yoo, Jong-Hyun;Kim, Ju-Won;Kim, Ha-Jung;Lim, Chae-Young;Lee, So-Young;Kim, Jung-Hyun;Woo, Eung-Je;Park, Hee-Myung
    • Journal of Veterinary Clinics
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    • v.24 no.4
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    • pp.479-485
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    • 2007
  • A retrospective study of 94 hypercalcemic dogs was performed to find out most common causes that lead to hypercalcemia through investigating dogs referred to the Veterinary Teaching Hospital of Konkuk University from 2002 to 2004. During the study period, hypercalcemia was found in 94 dogs of 19 breeds, and they were evaluated as case group. Control group was made up of 94 dogs of 18 breeds without hypercalcemia admitted for the same study period. For general signalments, there were no significant differences between case and control group with the exception of age distribution. Shih-tzu(17.02%) and Yorkshire terrier(26.60%) was the most common breed in case and control group, respectively. The most common diseases associated with hypercalcemia were chronic renal failure (18.09%), acute renal failure(14.89%), and renal calculi(6.38%). Malignant neoplasia(lymphoma, hemangiosarcoma, chronic lymphocytic leukemia, mammary gland tumor, and multiple myeloma) and endocrinopathies(hyperadrenocorticism, hyperthyroidism, hypoadrenocorticism, and hypothyroidism) occupied 8.5% and 6.4%, respectively. This report is a first retrospective study of hypercalcemic dogs in South Korea.

Perception on food allergy labelling and management of nutritional education among higher grade elementary school students in Jeju area (제주지역 초등학교 고학년 학생들의 식품알레르기 유발표시제에 대한 인식도 및 영양교육 운영 실태 조사)

  • Lee, Yunkyoung;Kim, Hyun-Hee;Ko, Yang-Sook
    • Journal of Nutrition and Health
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    • v.48 no.6
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    • pp.530-541
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    • 2015
  • Purpose: This study was conducted to examine the prevalence of food allergy, food allergy knowledge, and perception on food allergen labelling along with management of substitute meals in school foodservice in elementary school students in Jeju, South Korea. Methods: Six elementary schools in Jeju-si were randomly selected, and the higher grade elementary school students (n=1,500), from fourth graders to sixth graders, were surveyed using a questionnaire in July 2014. A total of 1,000 responses were used for data analysis. Results: The percentage of students who had experienced food allergy was 16.7%; 26.4% had been diagnosed as having a food allergy by doctors. Milk, crabs, and shrimps were the most frequent allergenic foods with common symptoms including urticaria followed by itching, and vomiting. As the student's grade went up, the knowledge scores on food allergy showed a significant increase. Although students with and without food allergy experience had a similar level of knowledge on food allergy and perception on food allergen labelling, students with food allergy experience had a significantly lower level of healthy dietary practices. Last, only 20.4% of students with food allergies were offered substitute meals by school foodservices, and 30.8% of students received food allergy education. Conclusion: For prevention and management of food allergies in school foodservice, schools should provide an individualized substitute meal plan and food allergy education to students, parents, and teachers.

Proximate Composition and Sterol Content of 35 Species of Marine Invertebrates (35종 해산 무척추동물의 일반성분조성과 스테롤함량)

  • JEONG Bo-Young;CHOI Byeong-Dae;MOON Soo-Kyung;Lee Jong-Soo;JEONG Woo-Geon;KIM Poong-Ho
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.32 no.2
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    • pp.192-197
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    • 1999
  • Proximate composition, sterol and $\alpha$-tocopherol ($\alpha$-Toc) contents of 35 species of marine invertebrates (13 species of Bivalvia, 10 species of Gastropoda, 4 species of Cephalopoda, 4 species of Crustacea, 2 species of Ascidacea and 2 species of Holothuroidea), which caught off the coast of Tongyeong of the Southern sea (Nam-Hae), Korea, were determined. Protein content showed relatively high level in herbivorous (seaweed feeder) and carnivorous species such as Gastropoda ($17.2\pm4.30\%$), Crustacea ($16.8\pm4.06\%$) and Cephalopoda ($15.3\pm3.24\%$), but showed low in plankton feeder and mud swallow such as Bivalvia ($11.8\pm2.49\%$), Ascidacea ($8.20\pm1.00\%$) and Holothuroidea ($3.94\pm1.13\%$). Total lipid (TL) content was low in most marine invertebrates, ranged from $0.24\%$ (blue colored sea cucumbers) to $1.96\%$ (sea squirts). Ascidacea contained the largest amount of TL in all samples, while Holothuroidea contained the smallest amount of TL, and other classes contained about $1\%$ of TL. Carbohydrates was rich in plankton feeder and Herbivorous species (seaweed feeder) such as Ascidacea ($6.60\pm4.53\%$), Bivalvia ($3.15\pm1.82\%$) and Gastropoda ($3.02\pm1.61\%$), while poor in carnivorous species such as Crustacea ($0.52\pm0.57\%$) and Cephalopoda ($1.00\pm0.63\%$). Moisture content was highest in Holothuroidea ($92.0\pm1.94\%$) and the lowest in Gastropoda ($77.0\pm1.95\%$): There were negative correlations between moisture and protein content, or TL, between protein and TL content; y= -0.8716x+184.452 (r=-0.87, p<0.001), $y=128.52e^{-0.0601x}$ (r=-0.55, P<0.001), $y=6.3047e^{-0.0904x}$(r=-0.42, P<0.02), respectively. Sterol content was ranged from 56 mg/100 g edible portion of red colored sea cucumbers to 216 mg/100 g edible portion of cockle and was high in the following order; Cephalopoda (148 $\pm$30.0mg)>Bivalvia (121$\pm$35.0mg)>Gastropoda (118$\pm$20.0mg)>Crustacea (116$\pm$31.0mg)>Ascidacea (78.0$\pm$2.00 mg)>Holothuroidea(62.0$\pm$8.00 mg). Crustacea contained 1$\~$2 mg $\alpha$-Toc/100 g edible portion whereas other classes contained trace or less than 1 mg/100 g of edible portion.

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Effects of Oyster-Shell Meal on Improving Spring Chinese Cabbage Productivity and Soil Properties (굴 패화석 비료가 배추 수량 및 토양 특성에 미치는 효과)

  • Lee, Ju-Young;Lee, Chang-Hoon;Yoon, Young-Sang;Ha, Byung-Hyun;Jang, Byoung-Choon;Lee, Ki-Sang;Lee, Do-Kyoung;Kim, Pil-Joo
    • Korean Journal of Soil Science and Fertilizer
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    • v.38 no.5
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    • pp.274-280
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    • 2005
  • Enormous amount of oyster-shell waste has been illegally disposed at oyster farm sites along the southern coast of Korea and then made a serious problem in environmental side. To increase the consumption of oyster shell meal as a soil amendment, the effects of oyster shell meal on soil properties and spring Chinese cabbage productivity were evaluated in silt loam soil to which 0, 4, 8, 12 and $16Mg\;ha^{-1}$ of oyster-shell meal fertilizer were added. Hydrated lime treatment ($2Mg\;ha^{-1}$) was selected as a control. Oyster-shell meal fertilizer, which made by a simple crushing and has high alkalinity as a calcium materials, had significant effects on neutralizing acid soil and on supplying calcium element. Spring Chinese cabbage yields increased with shell meal application. Plant uptakes of macro-nutrients such as P and C, and micro-nutrient such as B were significantly increased by oyster-shell meal application and then contributed to promote Chinese cabbage growth. The highest yield was achieved following the addition of $8Mg\;ha^{-1}$ shell meal application, and the same yield with that in lime treatment was at the $4Mg\;ha^{-1}$. Oyster-shell meal had more substantial effect on suppling calcium and on improving soil pH than that of lime. In conclusion, oyster shell meal fertilizer could be a good supplement to other inorganic soil amendments to improve nutrient balances in upland soils.

Disease Reaction of a Japonica Rice, Keumo3, and Detection of a Linked DNA Marker to Leaf Blast Resistance ("금오3호"의 벼 잎도열병 저항성 특성 및 저항성 연관 마커 탐색)

  • Lee, Jong-Hee;Kwak, Do-Yeon;Pakr, Dong-Soo;Roh, Jae-Hwan;Kang, Jong-Rae;Kim, Choon-Song;Jeon, Myeong-Gi;Yeo, Un-Sang;Yi, Gihwan;Shin, Mun-Sik;Oh, Byeong-Geun;Hwang, Hung-Goo
    • Korean Journal of Breeding Science
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    • v.40 no.4
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    • pp.408-413
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    • 2008
  • Rice blast resistance is considered one of the most important traits in rice breeding and the disease, caused by Magnaporthe grisea Barr, has brought significant crop losses annually. Moreover, breakdown of resistance normally occurs in two to five years after cultivar release, thus a more durable resistance is needed for better control of this disease. We developed a new variety, Keumo3, which showed strong resistance to leaf blast. It was tested in 2003 to 2007 at fourteen blast nursery sites covering entire rice-growing regions of South Korea. It showed resistance reactions in 12 regions and moderate in 2 regions without showing susceptible reactions. Durability test by sequential planting method indicated that this variety had better resistance. Results showed that Keumo3 was incompatible against 19 blast isolates with the exception of KI101 by artificial inoculation. To understand the genetic control of blast resistance in rice cultivar Keumo3 and facilitate its utilization, recombinant inbred lines (RIL) consisting of 290 F5 lines derived from Akidagomachi/Keumo3 were analyzed and genotyped with Pizt InDel marker zt56591. The recombination value between the marker allele of zt56591 and bioassay data of blast nursery test was 1.1%. These results indicated that MAS can be applied in selecting breeding populations for blast resistance using zt56591 as DNA marker.

진도의 담수산 물벼룩류와 요각류의 출현특성에 관한 생태학적 연구

  • Yoon, Seong-Myeong;Chang, Cheon-Young;Kim, Won
    • Animal Systematics, Evolution and Diversity
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    • v.11 no.1
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    • pp.39-64
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    • 1995
  • A faunistic and ecological study on the occurrence of freshwater cladocerans and copepods was accomplished from Chindo, South Korea. Collections were made from total 35 stations, comprising the various freshwater habitats like reservoirs, streams, swamps, bogs, ricefields, ditch, pond, and spring during the periods of July 23-25, and November 1-3 in 1994. Twenty seven cladoceran species of 17 genera of 6 families in 2 orders, and 28 copepod species of 21 genera of 6 families in 3 orders were collected during this research period, of which Daphnia obtusa Kurz and Elaphoidella bidens (Schmeil) are newly recorded from Korea. In reservoirs, Diaphanosoma sp. and Thermocyclops taihokuensis were dominant in July, and then succeeded by Bosmina longirostris and Cyclops vicinus vicinus in November. Thermocyclops crassus co-occurred with 7: taihokuensis at both seasons, was frequent in November after T. taihokuensis precipitately decreased. In other stagnant waters, 7: taihokuensis and Moina weismanni were dominant at ponds in July and in November, respectively. At ricefields in July Moina macrocopa and T. taihokuensis were dominant, but in November M. macrocopa and Paracyclops fimbriatus were. At streams, cladocerans were relatively rare, but became more rich in November. The representative cladoceran species were Bosmina longirostris as a plankton, and Chydorus sphaericus as a epibenthic species. Concerning copepods, nearly all the stations of streams except a few ones adjacent to seashore showed the similiar species constitutions, of which E. serrulatus and M, pehpeiensis were most frequent and abundant. At a mountain streamlet and a spring, the occurrence of Alona sp., Attheyella byblis Chang and Kim, 1992 and A. tetraspinosa Chang, 1993 is quite interesting and deserved much attention in the taxonomical point of view. Seventeen major cladocerans and copepods from lentic habitats and 13 major cladocerans and copepods from lotic habitatats were clustered using average taxonomic distance and UPGMA to infer the co-occurrence relations among species. As for lentic habitats, two large phena were appeared at first. The one phenon consisted of Diaphanosoma sp. and T taihokuensis, and showed its predominancy over the various habitats and its dominancy was rapidly decreased in November. The other phenon frequently occurred rather in November, and subdivided into three subgroups. On the other hand, as for lotic habitats, 13 species were also grouped into 2 large phena. The first one comprised 4 species, which were dominant and highly frequent at nearly all the lotic habitats, and subdivided into three subgroups according to their seasonal fluctuation types. The second one was also subdivided into three phena, the first of which comprised only one species, Microcyclops varicans, and occurred at most of the stations along stream with steadiness through the research period; the second phenon, Chydorus sphaericus, occurred much frequently in November; the last phenon included a few heterogenous subgroups.

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Characteristics of Particulate Carbon in the Ambient Air in the Korean Peninsula (한반도 권역별 대기 중 입자상 탄소 특성 연구)

  • Lee, Yeong-jae;Park, Mi-kyung;Jung, Sun-a;Kim, Sun-jung;Jo, Mi-ra;Song, In-ho;Lyu, Young-sook;Lim, Yong-jae;Kim, Jung-hoon;Jung, Hae-jin;Lee, Sang-uk;Choi, Won-Jun;Ahn, Joon-young;Lee, Min-hee;Kang, Hyun-jung;Park, Seung-myeong;Seo, Seok-jun;Jung, Dong-hee;Hyun, Joo-kyeong;Park, Jong-sung;Hwang, Tae-kyung;Hong, You-deog;Hong, Ji-hyung;Shin, Hye-jung
    • Journal of Korean Society for Atmospheric Environment
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    • v.31 no.4
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    • pp.330-344
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    • 2015
  • Semi-continuous measurements of $PM_{2.5}$ mass, organic and elemental carbon were made for the period of January to October 2014, at six national air monitoring stations in Korea. OC and EC concentrations showed a clear seasonal variation with the highest in winter (January) and the lowest in summer (August). In winter, the high carbonaceous concentrations were likely influenced by increased fuel combustion from residential heating. OC and EC concentrations varied by monitoring stations with 5.9 and $1.7{\mu}g/m^3$ in Joongbu area, 4.2 and $1.2{\mu}g/m^3$ in Honam area, 4.0 and $1.3{\mu}g/m^3$ in Yeongnam area, 3.7 and $1.6{\mu}g/m^3$ in Seoul Metropolitan area, 3.0 and $0.8{\mu}g/m^3$ in Jeju Island, 2.9 and $0.7{\mu}g/m^3$ in Baengnyeong Island respectively. The concentrations of OC and EC comprised 9.6~ 15.5% and 2.4~ 4.7% of $PM_{2.5}$. Urban Joongbu area located adjacent to the intersection of several main roads showed the highest carbon concentration among six national air monitoring station. On the other hand, background Baengnyeong Island showed the lowest carbon concentration and the highest OC/EC ratio (4.5). During the haze episode, OC and EC were enhanced with increase in $PM_{2.5}$ about 1.3~ 3 and 1.3~ 4.0 times respectively. The concentrations of OC, EC in the Asian dust case are about 1~ 2.4 times greater than in the nondust case. The origins of air mass pathways arriving at Seoul, using the backward trajectory analysis, can be mostly classified into 6 groups (Sector I Northern Korea including the sea of Okhotsk, Sector II Northern China including Mongolia, Sector III Southern China, Sector IV South Pacific area, Sector V Japan, Sector VI Southern Korea area). When an air mass originating from northern China and Mongolia, the OC concentrations were the most elevated, with a higher OC/EC ratio (2.4~ 3.3), and accounting for 17% of $PM_{2.5}$ mass on average.

Microbiological Safety of Various Gouda Cheeses Produced from Raw Milk (비살균 원유로 제조된 다양한 Gouda 치즈의 미생물 안전성)

  • Choi, Cheol;Kim, Dong-Hyeon;Lim, Hyun-Woo;Chon, Jung-Whan;Song, Kwang-Young;Kim, Se-Hyung;Kim, Hyunsook;Seo, Kun-Ho
    • Journal of Dairy Science and Biotechnology
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    • v.36 no.2
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    • pp.106-120
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    • 2018
  • Since 2018, the production and sales of ram-milk cheese ripened for over 60 days has been permitted in South Korea. Hence, this study aimed to examine the microbiological changes in 7 different types of Gouda cheese. During the aging period, traditional raw-milk Gouda Cheeses 1 and 2 did not contain Salmonella spp. during the 60-day storage period and no E. coli after 20-day storage. Coliform bacteria were not detected in Cheese 1 after 40 days; however, they were detected in Cheese 2 up to 60 days. Salmonella spp. were inhibited during the 60-day storage period in Cheese 3 (Salmonella spp.-contaminated raw-milk Gouda cheese), Cheese 4 (Cheese 3 contaminated with lactic acid bacteria DH 5 isolated from Kefir) and Cheese 5 (Cheese 3 contaminated with lactic acid bacteria DN1 isolated from Kefir). In particular, inhibition of Salmonella spp. was more prominent in Cheese 4 and Cheese 5 than in Cheese 3. During 60-day storage, Cheese 6 had a significantly reduced lactic acid bacteria. Furthermore, in Cheese 7, E. coli, E. Salmonella ssp. were rarely detected, and lactic acid bacteria were slightly greater in Cheese 7 than in other cheeses during the 60-day period. Moreover, all samples from Cheese 1 to Cheese 7 were not contaminated with Listeria monocytogenes, Staphylococcus aureus, Clostridium perfringens, and E. coli O157:H7.

Association of Melanocortin 4 Receptor (MC4R) Genotypes with Fatty Acid Compositions in an Intercross Population between Duroc and Jeju Native Pigs (제주재래돼지와 듀록 참조축군에서 Melanocortin 4 Receptor (MC4R) 유전자형과 지방산 조성간의 관련성 분석)

  • Kang, Yong-Jun;Kim, Sang-Geum;Kim, Su-Yeon;Shin, Moon-Cheol;Woo, Jae-Hoon;Kim, Nam-Young;Shin, Sang-Min;Choi, Jae-Young;Yoo, Ji-Hyun;Park, Nam-Geon;Yang, Byoung-Chul;Cho, In-Cheol
    • Journal of Life Science
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    • v.30 no.1
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    • pp.58-63
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    • 2020
  • This study was conducted to examine association between melanocortin-4-receptor (MC4R) genotypes and fatty acid (FA) composition in an F2 intercross between Duroc and Jeju (South Korea) Native pigs (JNP). Fourteen FA composition traits were measured in more than 290 F2 progeny population produced between Duroc and JNP. All experimental pigs were successfully genotyped for the MC4R c.1426A>G (p.Asp298Asn) single nucleotide polymorphism (SNP) by using Taq I PCR-RFLP methods. We detected three MC4R genotypes, AA, AG, and GG with 0.299, 0.542, and 0.159 genotype frequencies, respectively. The MC4R AA genotype animals showed higher levels in palmitic acid (C16:0, p<0.05), stearic acid (C18:0, p<0.01), eicosenoic acid (C20:1n9, p<0.05), saturated fatty acid (SFA, p<0.01) than GG homozygotes, respectively. Whereas MC4R GG genotype showed higher values in linoleic acid (C18:2n6, p<0.001), linolenic acid (C18:3n3, p<0.001), linolenic acid (C18:3n6, p<0.001), arachidonic acid (C20:4n6, p<0.001) and unsaturated fatty acid (USFA, p<0.01) than AA pigs, respectively. The MC4R GG genotype was associated with increasing USFA and decreasing SFA in the F2 progeny population produced between Duroc and JNP. Our finding suggests that the MC4R polymorphisms can be used as a valuable genetic marker for Duroc and JNP breeding programs to improve meat quality and to control FA compositions.