• BMB Reports
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• v.34 no.2
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• pp.109-113
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• 2001

The Serratia marcescens metalloprotease inhibitor (SmaPI) is a proteinase inhibitor toward Serratia marcescens metalloprotease (SMP). The three-dimensional structure of SmaPI was calculated by computer modeling using the structure complex between SMP and the Erwinia chrysanthemi inhibitor as a template. Based on this model structure, the substitution of the amino acid residues, Ala4, Leu-5, Pro-6, and Thr-7, were located at the hinge region of the N-terminal segment by site-directed mutagenesis. Although the A4R and T7A mutant SmaPIs showed a nearly full inhibitory activity, the inhibitory activity of SmaPI decreased significantly when the Leu-5 was converted to Ala, Gly, Ile, or Val. Surprisingly, the L5I and L5V mutant SmaPIs showed less inhibitory activities than the L5A mutant. From these results, we suggested that the orientations and positions of respective aliphatic groups in the side-chain of position 5 mainly affected the inhibitory activity of SmaPI. The overall side-chain hydrophobicity was only slightly affected. The side-chain of the Leu-5 residue contributed approximately 0.79 kcal/mol out of 8.44 kcal/mol to the binding of SmaPI with SMP The inhibitory activities of P6A and F6G were also severely decreased. The Pro-6 may have a critical role in maintaining the strict conformation of the N-terminal portion that may be important in the inhibitory activity of SmaPI. In conclusion, Leu-5 and Pro-6 have crucial roles in the inhibitory function of SmaPI toward SMP.

• Journal of Plant Biotechnology
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• v.42 no.3
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• pp.235-238
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• 2015

Adventitious buds were obtained from isolated cotyledons cultured on MS medium with various concentrations of 6-benzylamino purine (BA) and thidiazuron (TDZ). The highest numbers of adventitious buds were obtained on MS medium supplemented with 0.2 mg/L BA. Experimental culturing with half the petiole portion and half with the terminal segments were grown on MS medium contained with 0.2 mg/L BA. Frequency of the adventitious bud induction was variable accordingly to the type of cultured explants. Explants with the half petiole showed the highest adventitious bud induction rate (80%) compared to explants of half with terminal segment (20%). An elongated shoot from the buds and growth of advent roots were both possible on the 1/2 MS medium without a plant growth regulator. These results offer an effective way in which clonal propagation can be accomplished.

• BMB Reports
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• v.42 no.9
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• pp.552-560
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• 2009

Cyclooxygenases (COX-1 and COX-2) are ER-resident proteins that catalyze the committed step in prostanoid synthesis. COX-1 is constitutively expressed in many mammalian cells, whereas COX-2 is usually expressed inducibly and transiently. Abnormal expression of COX-2 has been implicated in the pathogenesis of chronic inflammation and various cancers; therefore, it is subject to tight and complex regulation. Differences in regulation of the COX enzymes at the posttranscriptional and posttranslational levels also contribute significantly to their distinct patterns of expression. Rapid degradation of COX-2 mRNA has been attributed to AU-rich elements (AREs) at its 3’UTR. Recently, microRNAs that can selectively repress COX-2 protein synthesis have been identified. The mature forms of these COX proteins are very similar in structure except that COX-2 has a unique 19-amino acid (19-aa) segment located near the C-terminus. This C-terminal 19-aa cassette plays an important role in mediation of the entry of COX-2 into the ER-associated degradation (ERAD) system, which transports ER proteins to the cytoplasm for degradation by the 26S proteasome. A second pathway for COX-2 protein degradation is initiated after the enzyme undergoes suicide inactivation following cyclooxygenase catalysis. Here, we discuss these molecular determinants of COX-2 expression in detail.

• BMB Reports
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• v.44 no.10
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• pp.669-673
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• 2011

Human methionine sulfoxide reductase B3A (hMsrB3A) is an endoplasmic reticulum (ER) reductase that catalyzes the stereospecific reduction of methionine-R-sulfoxide to methionine in proteins. In this work, we identified an antimicrobial peptide from hMsrB3A protein. The N-terminal ER-targeting signal peptide (amino acids 1-31) conferred an antimicrobial effect in Escherichia coli cells. Sequence and structural analyses showed that the overall positively charged ER signal peptide had an Argand Pro-rich region and a potential hydrophobic ${\alpha}$-helical segment that contains 4 cysteine residues. The potential ${\alpha}$-helical region was essential for the antimicrobial activity within E. coli cells. A synthetic peptide, comprised of 2-26 amino acids of the signal peptide, was effective at killing Gram-negative E. coli, Klebsiella pneumoniae, and Salmonella paratyphi, but had no bactericidal activity against Gram-positive Staphylococcus aureus.

• Animal Systematics, Evolution and Diversity
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• v.19 no.2
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• pp.177-188
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• 2003

Two new species of the genus Orecturus are described os associates of octocorals in Cheju Island, Korea. Orecturus longicaudatus n. sp. associated with a gorgonacean coral differs from its congeners by having a large body and a long anal somite. Orecturus similis n. sp., associated with an alcyonacean coral, is similar to O. excavatus but can be distinguished from it by possessing a single seta on the second segment of maxilla, the terminal claw of maxilliped which is evenly tapering, and the different ornamentation in legs 1 and 4.

• Journal of Species Research
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• v.5 no.1
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• pp.49-156
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• 2016

Fourteen new species and A. coreana Morimoto, 1970 of Allobathynella Morimoto and Miura, 1957 are (re-)described and illustrated from the Korean Penninsula. Comparison of the external morphology of these species and two previously known species, A. japonica Morimoto and Miura, 1957 and A. shinjongieei Park and Cho, 2008 enables us to amend the generic diagnosis. A 7-segmented antennule turns out to be inconsistent character due to A. wonjuensis sp. nov., which has a 6-segmented antennule. On the other hand, the exopodal distal segment of thoracopods I-VII with a tiny terminal knob and with outer seta covered by long and strong barbs is proved to be a autapomorphic character of the genus. The 14 new species belong to the mirabilis-group and display more or less similar morphology. However, they differ from A. coreana, A. japonica and A. shinjongieei and from each other not only in quantitative but also in qualitative characters. These differences are summarized in tables. The species inhabit hyporheic zones of rivers and each appears to be highly endemic to a given tributary, having a range limit less than 100 km.

• Journal of Advanced Navigation Technology
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• v.12 no.2
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• pp.164-169
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• 2008

This paper is using motion tracking by image segmentation to monitor intruders and to confirm based on mobile the relevant information. First, detect frame in animation that film fixed area, and make use of image subtraction between two frame that adjoin, segment fixed backing and target who move. Segmental foreground object to the edge detecting the location specified by the edge of the median estimate extracted by analyzing the motion of the intruders to monitor. When a motion is detected, the detected image is transmitted by using the W AP pull basis image transmission method on the mobile client data terminal.

• Animal Systematics, Evolution and Diversity
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• v.21 no.2
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• pp.193-199
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• 2005

The first zoea of Liomera bella belonging to the subfamily Liomerinae is described and illustrated for the first time. Its morphological characteristics are compared with those of other known species of the Xanthidae The general morphology of it corresponds well with zoeas of the Xanthidae. Based on the zoeal morphology, the zoea of L. bella shows a greatest affinity with those of Xontho incisus and Pseudomedaeus agassizii by having the exopod of antenna with two setae, the terminal segment of the endopod of the second maxilliped with six setae, and the fork of telson with one stout and one smaller lateral spines.

• Advances in pediatric surgery
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• v.4 no.1
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• pp.74-78
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• 1998

Early recognition and surgical treatment of Hirschsprung's disease prevents serious mortality and morbidity from enterocolitis and obstruction. Usually this disease is characterized by a single aganglionic segment of the colon extending distally to the anal margin. In surgical treatment, the surgeon performs a frozen section biopsy to confirm whether there are ganglion cells. If there are intervening ganglionic sites in aganglionic bowel, there may be confusion in diagnosis and treatment. The authors experienced one case of total colonic aganglionosis with skip area. A transverse loop colostomy was performed on a 7 day-old male baby with colon perforation due to Hirschsprung's disease. But intestinal obstruction persisted and required two more operations to find the true nature of the disease. There were aganglionic segments from the anal margin to the terminal ileum 3.7cm proximal to the ileocecal valve. The entire transverse colon and appendix were normally ganglionated.

• Animal Systematics, Evolution and Diversity
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• v.31 no.1
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• pp.31-41
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• 2015

A new species of the genus Farranula Wilson, 1942 (Cyclopoida, Corycaeidae) is described based on both sexes collected off Chuuk Island in Micronesia (West Central Pacific). The new species F. dahlae differs from its close congener F. gibbula (Giesbrecht, 1891) in the following combination of characters in both sexes: body length is longer, length to width ratio of caudal rami is larger, basal element of maxilliped is distinctly longer, and terminal spine to distal segment ratio of P4 is smaller; while in females, lateral margins of fourth pedigerous somite are extended to mid-region of second urosomal somite, maximum width of the second urosomal somite is located at middle region in dorsal and lateral views, and length ratio of caudal seta III to seta V is much larger; and in males, sharply contracted portion of second somite is located at two-thirds distance from anterior margin. Some additional morphological details of F. gibbula are given and a key to species of the genus Farranula is provided.