• Journal of the Microelectronics and Packaging Society
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• v.25 no.3
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• pp.13-19
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• 2018

The soldering property of Pb-containing solder(Sn-Pb) and Pb-free solders(Sn-3.0Ag-0.5Cu and Sn-1.0Ag-0.7Cu-1.6Bi-0.2In) for solar combiner box module was compared. The solar combiner box module was composed of voltage and current detecting modules, diode modules, and other modules. In this study, solder paste printability, printing shape inspection, solder joint property, X-ray inspection, and shear force measurements were conducted. For optimization of Pb-free soldering process, step 1 and 2 were divided. In the step 1 process, the printability of Pb-containing and Pb-free solder alloys were estimated by using printing inspector. Then, the relationship between void percentages and shear force has been estimated. Overall, the property of Pb-containing solder was better than two Pb-free solders. In the step 2 process, the property of reflow soldering for the Pb-free solders was evaluated with different reflow peak temperatures. As the peak temperature of the reflow process gradually increased, the void percentage decreased by 2 to 4%, but the shear force did not significantly depend on the reflow peak temperature by a deviation of about 0.5 kgf. Among different surface finishes on PCB, ENIG surface finish was better than OSP and Pb-free solder surface finishes in terms of shear force. In the thermal shock reliability test of the solar combiner box module with a Pb-free solder and OSP surface finish, the change rate of electrical property of the module was almost unchanged within a 0.3% range and the module had a relatively good electrical property after 500 thermal shock cycles.

• Journal of Bio-Environment Control
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• v.22 no.2
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• pp.138-145
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• 2013

In order to develop the efficient control algorithm of the two-fluid fogging system, cooling experiments for the many different types of fogging cycles were conducted in tomato greenhouses. It showed that the cooling effect was 1.2 to $4.0^{\circ}C$ and the cooling efficiency was 8.2 to 32.9% on average. The cooling efficiency with fogging interval was highest in the case of the fogging cycle of 90 seconds. The cooling efficiency showed a tendency to increase as the fogging time increased and the stopping time decreased. As the spray rate of fog in the two-fluid fogging system increased, there was a tendency for the cooling efficiency to improve. However, as the inside air approaches its saturation level, even though the spray rate of fog increases, it does not lead to further evaporation. Thus, it can be inferred that increasing the spray rate of fog before the inside air reaches the saturation level could make higher the cooling efficiency. As cooling efficiency increases, the saturation deficit of inside air decreased and the difference between absolute humidity of inside and outside air increased. The more fog evaporated, the difference between absolute humidity of inside and outside air tended to increase and as the result, the discharge of vapor due to ventilation occurs more easily, which again lead to an increase in the evaporation rate and ultimately increase in the cooling efficiency. Regression analysis result on the saturation deficit of inside air showed that the fogging time needed to change of saturation deficit of $10g{\cdot}kg^{-1}$ was 120 seconds and stopping time was 60 seconds. But in order to decrease the amplitude of temperature and to increase the cooling efficiency, the fluctuation range of saturation deficit was set to $5g{\cdot}kg^{-1}$ and we decided that the fogging-stopping time of 60-30 seconds was more appropriate. Control types of two-fluid fogging systems were classified as computer control or simple control, and their control algorithms were derived. We recommend that if the two-fluid fogging system is controlled by manipulating only the set point of temperature, humidity, and on-off time, it would be best to set up the on-off time at 60-30 seconds in time control, the lower limit of air temperature at 30 to $32^{\circ}C$ and the upper limit of relative humidity at 85 to 90%.

• Korean Journal of Environmental Biology
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• v.38 no.1
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• pp.127-136
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• 2020

This study was conducted to investigate the population characteristics of Nile tilapia (Oreochromis niloticus) in the Daegu Metropolitan City thermal effluent stream (Dalseo Stream) from January to November 2019. The collected fish were identified as 4,247 individuals of 20 species from a total of eight families. The dominant species was O. niloticus with 1,306 individuals and a high relative abundance (30.75%). The water temperature of Dalseo Stream was maintained above 10℃ throughout the year, which means that O. niloticus could inhabit it even in winter. The length-weight analysis showed a regression coefficient b of 3.1496, and a condition factor (k) of 0.0025 with a positive slope. Comparing the water temperature of Dalseo Stream and the total length of O. niloticus per investigation period, the 0-age individuals appeared May 29 when the water temperature was maintained above 22℃. In conclusion, the thermal effluent of Dalseo Stream allowed O. niloticus to survive in winter and maintain stable growth conditions and life cycles. The results of this study will inform ecological information on O. niloticus, which suggests that river management efforts should consider the management of O. niloticus populations for the conservation of fish species diversity.

• Development and Reproduction
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• v.10 no.4
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• pp.263-269
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• 2006

To evaluate the potentiality of industrial use of cysteine proteinase inhibitor (cystatin) of tilapia egg and serum stability of the tilapia cystatin on low temperature storage and heat treatment was studied. When the eggs were stored at $4^{\circ}C$ for 3 days the cystatin activity was not changed much, while the supernatant of egg homogenate lost its cystatin activity significantly, remaining only about 65% of initial activity. When the eggs and serum were subjected to repeated freeze at $-20^{\circ}C$ and thaw at room temperature once a day, the egg cystatin was decreased after 5 cycles of freeze and thaw. However the serum cystatin was not changed by the 5 times repetition of freeze and thaw. More than 80% of egg cystatin activity was remained when the egg was heated at $35^{\circ}C$ for 30 min, but less than 10% was remained when heated at $50^{\circ}C$. On the other hand, the serum cystatin was very resistant to heat, remaining about 74% after heating at as high as $80^{\circ}C$ for 30 min. In summary, the egg cystatin was more stable when stored as intact form of egg rather than as supernatant of homogenate when stored at refrigeration. Egg cystatin was relatively stable against repeated freeze-thaw, and serum was found to be more stable in cysteine proteinase inhibitory activity than egg. Egg cystatin was not very resistant to heat treatment, while serum cystatin was quite resistant to high temperature heat treatment. These results suggest that tilapia egg and serum, especially the serum, would be a useful source for cysteine proteinase inhibitor in surimi production.

• Proceedings of the Korean Vacuum Society Conference
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• 2013.08a
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• pp.88-89
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• 2013

A variety of influenza A viruses from animal hosts are continuously prevalent throughout the world which cause human epidemics resulting millions of human infections and enormous industrial and economic damages. Thus, early diagnosis of such pathogen is of paramount importance for biomedical examination and public healthcare screening. To approach this issue, here we propose a fully integrated Rotary genetic analysis system, called Rotary Genetic Analyzer, for on-site detection of influenza A viruses with high speed. The Rotary Genetic Analyzer is made up of four parts including a disposable microchip, a servo motor for precise and high rate spinning of the chip, thermal blocks for temperature control, and a miniaturized optical fluorescence detector as shown Fig. 1. A thermal block made from duralumin is integrated with a film heater at the bottom and a resistance temperature detector (RTD) in the middle. For the efficient performance of RT-PCR, three thermal blocks are placed on the Rotary stage and the temperature of each block is corresponded to the thermal cycling, namely $95^{\circ}C$ (denature), $58^{\circ}C$ (annealing), and $72^{\circ}C$ (extension). Rotary RT-PCR was performed to amplify the target gene which was monitored by an optical fluorescent detector above the extension block. A disposable microdevice (10 cm diameter) consists of a solid-phase extraction based sample pretreatment unit, bead chamber, and 4 ${\mu}L$ of the PCR chamber as shown Fig. 2. The microchip is fabricated using a patterned polycarbonate (PC) sheet with 1 mm thickness and a PC film with 130 ${\mu}m$ thickness, which layers are thermally bonded at $138^{\circ}C$ using acetone vapour. Silicatreated microglass beads with 150~212 ${\mu}L$ diameter are introduced into the sample pretreatment chambers and held in place by weir structure for construction of solid-phase extraction system. Fig. 3 shows strobed images of sequential loading of three samples. Three samples were loaded into the reservoir simultaneously (Fig. 3A), then the influenza A H3N2 viral RNA sample was loaded at 5000 RPM for 10 sec (Fig. 3B). Washing buffer was followed at 5000 RPM for 5 min (Fig. 3C), and angular frequency was decreased to 100 RPM for siphon priming of PCR cocktail to the channel as shown in Figure 3D. Finally the PCR cocktail was loaded to the bead chamber at 2000 RPM for 10 sec, and then RPM was increased up to 5000 RPM for 1 min to obtain the as much as PCR cocktail containing the RNA template (Fig. 3E). In this system, the wastes from RNA samples and washing buffer were transported to the waste chamber, which is fully filled to the chamber with precise optimization. Then, the PCR cocktail was able to transport to the PCR chamber. Fig. 3F shows the final image of the sample pretreatment. PCR cocktail containing RNA template is successfully isolated from waste. To detect the influenza A H3N2 virus, the purified RNA with PCR cocktail in the PCR chamber was amplified by using performed the RNA capture on the proposed microdevice. The fluorescence images were described in Figure 4A at the 0, 40 cycles. The fluorescence signal (40 cycle) was drastically increased confirming the influenza A H3N2 virus. The real-time profiles were successfully obtained using the optical fluorescence detector as shown in Figure 4B. The Rotary PCR and off-chip PCR were compared with same amount of influenza A H3N2 virus. The Ct value of Rotary PCR was smaller than the off-chip PCR without contamination. The whole process of the sample pretreatment and RT-PCR could be accomplished in 30 min on the fully integrated Rotary Genetic Analyzer system. We have demonstrated a fully integrated and portable Rotary Genetic Analyzer for detection of the gene expression of influenza A virus, which has 'Sample-in-answer-out' capability including sample pretreatment, rotary amplification, and optical detection. Target gene amplification was real-time monitored using the integrated Rotary Genetic Analyzer system.

• Journal of the Korean Electrochemical Society
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• v.17 no.1
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• pp.49-56
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• 2014

As an electrolyte additive, the effects of lithium bis(oxalate)borate (LiBOB) on the electrochemical properties of a carbon-coated silicon monoxide (C-coated SiO) negative electrode are investigated. The used electrolyte is 1.3M $LiPF_6$ that is dissolved in ethylene carbonate (EC), fluoroethylene carbonate (FEC), and diethyl carbonate (DEC) (5:25:70 v/v/v) with or without 0.5 wt. % LiBOB. In the LiBOB-free electrolyte, the film resistance is not so high in the initial period of cycling that lithiation is facilitated to generate the crystalline $Li_{15}Si_4$ phase. Due to repeated volume change that is caused by such a deep charge/discharge, cracks form in the active material to cause a resistance increase, which eventually leads to capacity fading. When LiBOB is added into the electrolyte, however, more resistive surface film is generated by decomposition of LiBOB in the initial period. The crystalline $Li_{15}Si_4$ phase does not form, such that the volume change and crack formation are greatly mitigated. Consequently, the C-coated SiO electrode exhibits a better cycle performance in the later cycles. At an elevated temperature ($45^{\circ}C$), wherein the effect of film resistance is less critical, the alloy ($Li_{15}Si_4$ phase) formation is comparable for the LiBOB-free and added cell to give a similar cycle performance.

• Clean Technology
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• v.22 no.3
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• pp.175-180
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• 2016

Tyrosinases catalyze the hydroxylation of a monophenol (monophenolase activity) and the conversion of an o-diphenol to o-quinone (diphenolase activity), which are mainly involved in the modification of tyrosine residues into 3,4-dihydroxyphenyl-alanine (DOPA) and DOPA/DOPAquinone-derived intermolecular cross-linking. Previously, we obtained a slightly acidic and cold-active tyrosinase, tyrosinase-CNK, by our recombinant protein approach. The enzyme showed optimal activity at pH 6.0 and 20 ℃ with an abnormally high monophenolase/diphenolase activity ratio and still had approximately 50% activity compared with the highest activity even in ice water. Here, we investigated reaction stability of the recombinant tyrosinase-CNK as a psychrophilic enzyme. The enzyme showed remarkable thermal stability at 0 ℃ and the activity was well conserved in repeated freeze-thaw cycles. Although water-miscible organic solvent as reaction media caused the activity decrease of tyrosinase-CNK as expected, the enzyme activity was not additionally decreased with increased concentration in organic solvents such as ethanol and acetonitrile. Also, the enzyme showed high salt tolerance in chaotropic salts. It was remarkably considered that 2⁺ metal ions might inhibit the incorporation of Cu²⁺ into the active site. We expect that these results could be used to design tyrosinase-mediated enzymatic reaction at low temperature for the production of catechols through minimizing unwanted self-oxidation and enzyme inactivation.

• International Journal of Highway Engineering
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• v.18 no.6
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• pp.123-130
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• 2016

OBJECTIVES : The objective of this research is to determine the moisture resistance of the freeze-thaw process occurring in low-noise porous pavement using either hydrated-lime or anti-freezing agent. Various additives were applied to low-noise porous asphalt, which is actively paved in South Korea, to overcome its disadvantages. Moreover, the optimum contents of hydrated-lime and anti-freezing agent and behavior properties of low-noise porous asphalt layer are determined using dynamic moduli via the freeze-thaw test. METHODS : The low-noise porous asphalt mixtures were made using gyratory compacters to investigate its properties with either hydrated-lime or anti-freezing agent. To determine the dynamic moduli of each mixture, impact resonance test was conducted. The applied standard for the freeze-thaw test of asphalt mixture is ASTM D 6857. The freeze-thaw and impact resonance tests were performed twice at each stage. The behavior properties were defined using finite element method, which was performed using the dynamic modulus data obtained from the freeze-thaw test and resonance frequencies obtained from non-destructive impact test. RESULTS : The results show that the coherence and strength of the low-noise porous asphalt mixture decreased continuously with the increase in the temperature of the mixture. The dynamic modulus of the normal low-noise porous asphalt mixture dramatically decreased after one cycle of freezing and thawing stages, which is more than that of other mixtures containing additives. The damage rate was higher when the freeze-thaw test was repeated. CONCLUSIONS : From the root mean squared error (RMSE) and mean percentage error (MPE) analyses, the addition rates of 1.5% hydrated-lime and 0.5% anti-freezing agent resulted in the strongest mixture having the highest moisture resistance compared to other specimens with each additive in 1 cycle freeze-thaw test. Moreover, the freeze-thaw resistance significantly improved when a hydrated-lime content of 0.5% was applied for the two cycles of the freeze-thaw test. Hence, the optimum contents of both hydrated-lime and anti-freezing agent are 0.5%.

• Journal of the Korean Society for Nondestructive Testing
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• v.36 no.4
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• pp.281-287
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• 2016

Fiber Bragg grating (FBG) sensors are applied in structural health monitoring (SHM) in various application fields because of their ease of multiplexing and capability of performing absolute measurements. Moreover, the packaging methods of FBG sensors accelerate their commercialization rapidly. However, long-term SHM exposes the FBG sensors to cyclic thermal loads, and a investigation is required because it finally leads to the signal instability of the FBG sensors. In this study, the effects of sensor packaging methods two methods are generally used for the FBGs: (bonding both sides of the FBG or bonding the FBG directly on signal stability of FBG sensors are investigated. Tests are conducted on specimens in a thermal chamber, over a temperature range from $-20^{\circ}C$ to $60^{\circ}C$ for 300 cycles. Signal characteristics such as Bragg wavelength, light intensity and full width at half maximum are examined and are compared with those of the FBG sensors, obtained in a previous study under direct bonding conditions. From the comparison, it is observed that the FBG sensors with bonding on both sides of the FBG demonstrate higher signal stabilities when exposed to cyclic thermal loads during long-term SHM. Consequently, it guarantees more effectiveness when packaging the FBG sensors.

• Horticultural Science & Technology
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• v.16 no.1
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• pp.21-24
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• 1998

RAPD markers were analyzed in order to detect the genetic variation and diversity of the fifty-two melon lines. SDS extraction method produced more and purer DNA than CTAB method. RAPD reaction conditions were optimized as follows ; 10ng template DNA, 270nM primer, $200{\mu}M$ each of dATP, dCTP, dGTP and dTTP, $0.3{\mu}unit$ dynazyme and 10x buffer brought to $15{\mu}l$ final volume with distilled water. The adequate annealing temperature was $39^{\circ}C$ and forty cycles of amplification produced the best RAPD band patterns. Among a total of 123 bands from 12 random primers, 25 polymorphic bands(20%) were selected as reliable markers. The average number of polymorphic bands per primer was 2.1 among the 52 lines. Intragroup genetic relationship based on the marker difference was closer than intergroup genetic relationship. The 52 lines could be grouped into two major group (Korean landraces and melon lines) and then melon group subdivided into two subgroups (net melon lines and no-net melon). This result corresponded to morphological grouping. Eight RAPD markers separated the Korean landraces and melon groups and four RAPD markers separated net melon and no-net melon groups.