• Title/Summary/Keyword: taxonomic studies

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Systematic Studies on Korean Rodents : VIII. Analyses of Morphometric Characters, Chromosomal Karyotype, and Mitochondrial DNA Restriction Fragments in Siberian Chipmunks from Korea (Tamias sibiricus barberi Johnson and Jones), with the Comparison of Morphometric Characters of Siberian Chipmunks from Manchuria (Tamias sibiricus orientalis Bonhote) (한국산 설치류의 계통분류학적 연구: 8. 다람쥐(Tamias sibiricus barberi Johnson and Jones)의 형태적 형질, 염색체 핵형 및 미토콘드리아 DNA절단 단편의 분석과 만주다람쥐(Tamias sibiricus orientalis Bonhote)와의 형태적 형질의 비교)

  • Hung Sun Koh
    • Animal Systematics, Evolution and Diversity
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    • v.10 no.2
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    • pp.231-243
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    • 1994
  • Samples of Siberian chipmunks (Tamias sibiricus borberi ) from Korea were analyzed by multivariate, air-drying , and blot-hybridization methods in order to determine the degree of variations of morphological characters, chromosomal karyotype, and mtDNA restriction fragment patterns, respectively, Moreover, morphometric characters of samples of Siberian chipmunks from Manchuria(T.sibiricus orientalis) were also analyzed in order to clarify the taxonomic status of the subspecies barberi. Siberian chipmunks from six localities in Korea were in morphometric characters more or less similar with one another and Siberian chipmunks from Manchuria were not so distinct enough to from a subgroup. Siberian chipmunks from Four localities in Korea were identical in their karyotypes (2n=38) : samples from three localities in Korea were nore or less similar in their mtDNA fragment patterns. Siberian chipmunks from Korea appeared to be a omogeneous population and as noted by Corbet (1978) subspecies barberi is the synonym of subspecies orientalis. The scientific name of Siberian chipmunks from Korea and Manchuria is T. sibiricus orientalis, although systematic studies with the samples from North korea, Manchuria and China will be necessary for their taxonomic reconsideration.

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Taxonomic Examination of Isoëtes L. (Isoëtaceae) in South Korea (우리나라 물부추속 (물부추과)에 대한 분류학적 고찰)

  • Jung, Jongduk;Kim, Changkyun;Kim, Hojun;Choi, Hong-Keun
    • Korean Journal of Plant Taxonomy
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    • v.39 no.2
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    • pp.63-73
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    • 2009
  • $Iso{\ddot{e}}tes$ L. ($Iso{\ddot{e}}taceae$) is difficult to identify among the infrageneric taxa because of morphological simplicity. Since two species (I. japonica and I. coreana) had been reported, I. jejuensis and I. hallasanensis were described as two new species from Jeju Island, South Korea. The habitats of all species were confirmed by current studies except for I. japonica. We compared the morphological and anatomical characters of seven species, three domestic species and four species in adjacent regions (I. taiwanensis, I. sinensis, I. japonica, and I. asiatica). I. asiatica differs from other species in the lobe number of corm, spreading sporophylls, lack of stomata and degree of velum development. All of the seven species were identified by the characteristics of their mega- and microspores. Especially, it was possible to identify the four species in South Korea by the types of ornamentation on their megaspores. Each species has a specific type of megaspore ornamentation: I. coreana - cristate, I. jejuensis - rugulate, I. hallasanensis - echinate, and I. japonica - reticulate. We also discussed the phylogeny of the genus in East Asia in relation to recent studies based on the second intron of LEAFY sequence and amplified fragment length polymorphism (AFLP) analysis. We suggest strategies for the conservation of the endangered $Iso{\ddot{e}}tes$ species in South Korea.

Differences in fecal and cecal microbiota in C57BL/6J mice fed normal and high fat diet (고지방 식이 조절에 따른 C57BL/6J 마우스의 분변과 맹장에서 나타나는 미생물생태 차이)

  • Lee, Sunwoo;Vineet, Singh;Unno, Tatsuya
    • Journal of Applied Biological Chemistry
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    • v.64 no.4
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    • pp.399-405
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    • 2021
  • A number of studies have been conducted to prevent obesity due to the worldwide increasing rate of obesity and its adverse effects on our health. Recently, a relationship between obesity and gut microbiome has been reported. Fecal and cecal microbiota are generally targeted for examining the gut microbiome during dietary interventions. There is, however, no common understanding on which microbiota and how results elucidated from the data would differ. In this study, we conducted dietary induced obesity study and compared fecal and cecal microbiota affected by dietary interventions. Normal Diet and high fat diet were fed to 6 weeks old mice for 12 weeks, and 16 S rRNA genes amplified from fecal and cecal DNA were sequenced using MiSeq. Our results show that the 𝛼-diversity showed significant differences between the dietary interventions as well as cecal and fecal microbiota. The difference in the taxonomic compositions between cecal and fecal microbiota had become clearer at the family and genus level. At the genus level, Faecalibaculum and Lactobacillus were more abundant in the cecal and fecal microbiota, respectively. In general dietary intervention studies, dietary effects are more significant than type difference. However, the microbiota analysis results should be interpreted carefully, considering both diet and samples (feces/caecum).

A report of 43 unrecorded bacterial species within the phyla Bacteroidetes and Firmicutes isolated from various sources from Korea in 2019

  • Kang, Heeyoung;Kim, Haneul;Yi, Hana;Kim, Wonyong;Yoon, Jung-Hoon;Im, Wan-Taek;Kim, Myung Kyum;Seong, Chi Nam;Kim, Seung Bum;Cha, Chang-Jun;Jeon, Che Ok;Joh, Kiseong
    • Journal of Species Research
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    • v.10 no.2
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    • pp.117-133
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    • 2021
  • In 2019, 43 bacterial strains were isolated from food, soil, marine environments, human, and animals related sources from the Republic of Korea. Based on the analysis of 16S rRNA gene sequence, these isolates were allocated to the phyla Bacteroidetes and Firmicutes as unrecorded species in Korea. The 10 Bacteroidetes strains were classified into the families Bacteroidaceae, Chitinophagaceae, Cytophagaceae, Flavobacteriaceae, and Prolixibacteraceae (of the orders Bacteroidales, Chitinophagales, Cytophagales, Flavobacteriales, and Marinilabiliales, respectively). The 33 Firmicutes strains belonged to the families Bacillaceae, Paenibacillaceae, Planococcaceae, Staphylococcaceae, Clostridiaceae, Lachnospiraceae, Peptostreptococcaceae, Enterococcaceae, Lactobacillaceae, Leuconostocaceae, and Streptococcaceae (of the orders Bacillales, Clostridiales, and Lactobacillales). These unrecorded bacteria were determined based on taxonomic criterion (>98.7%; 16S rRNA gene sequence similarity). In addition, their phylogenetic affiliation, as well as cell and colony morphologies, staining reactions, and physiological and biochemical properties were investigated. Therefore, we report 43 isolates as unrecorded species, and described basic features, isolation source, and locations of these strains.

Studies on the Film-Forming Yeast Isolated from Apple Wine -(I) Isolation and Identification of Yeast Strain (사과주(酒)에서 분리(分離)한 산막효모(産膜酵母)에 관(關)한 연구(硏究) -(1) 균주(菌株)의 분리(分離) 및 동정(同定))

  • Chung, Ki Taek;Song, Hyung Ik
    • Current Research on Agriculture and Life Sciences
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    • v.1
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    • pp.159-163
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    • 1983
  • Film-formation, which often occurs during storage of apple wine, owing to contamination by film-forming yeasts, results in inferior products. Therefore, for the purpose of preventing this occurrence, we isolated and identified yeast strain. Among the total number of 45 yeast strains which were isolated from contaminated apple wine in winery, the strains FY-4 and FY-5 were found to be useful. The strain FY-5, which greatly formed film on apple wine, was identified as Hansenula beijerinckii or similar strain according to taxonomic characteristics.

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Mitochondrial Genetic Variation of Pen Shell, Atrina pectinata in Korea and Japan

  • Kim, Dongsung;Rho, Hyun Soo;Jung, Jongwoo
    • Animal Systematics, Evolution and Diversity
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    • v.33 no.3
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    • pp.169-175
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    • 2017
  • In the northwestern Pacific region, the pen shell (Atrina pectinata) is a widely distributed bivalve and economically important in fisheries. Recently, stock of this species has been greatly reduced due to overexploitation and marine pollution, which arouses interest in conservation. Studies on genetic and taxonomic entities of pen shells have not been tried in Korea, which makes difficult to take measures for effective conservation of this marine resource. In this study, we investigated mitochondrial genetic polymorphism of pen shells collected from 4 locations in Korea and Japan using cytochrome c oxidase I (COI) gene sequences. A total of 39 haplotypes were identified among 86 individuals of pen shell. Although only 5 haplotypes were shared, no significant genetic differentiation was observed between Korean and Japanese populations. These results suggest that pen shell populations of these regions share an ancestral population which might have experienced expansion during the Pleistocene, but gene flow must have been highly restricted after expansion.

Taxonomic Studies on Spider Mites (Tetranichidas: Acarina) of III. Spider Mites Parasitic on Conifers (한국산 응애과(거미강: 진드기목)의 분류학적 연구 3. 침엽수에 기생하는 응애류)

  • Lee, Won-Koo;Lee, Byung-Hoon;Kim, Byung-Jin
    • Animal Systematics, Evolution and Diversity
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    • v.5 no.1
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    • pp.13-24
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    • 1989
  • Specimens of spider mites parasiting on conifer trees were collected from Korea during the period from May, 1986 to June, 1988. As a result, seven species belonging to the tribe Tetranychini, subfamily Tetranychinae, family Tetranychidae were identified as follows: 1. Panonychus citri(McGregor, 1916), *2. Oligonychus clavatus(Ehara,1959), *. O.hondoensis(Ehara,1954), *4. O.pustulosus Ehara, 1962, 5. O.karamatus(Ehara, 1956), 6. O.perditus Pritchard and Baker, 1955, 7. O. ununguis(Jacobi, 1905). Of which, three species with asterisk are newly recorded from Korea.

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Nuclear DNA Quantification of Some Ceramialean Algal Spermatia by Fluorescence Microscopic Image Processing and their Nuclear SSU rDNA Sequences

  • Choi, Han-Gu;Lee, Eun-Young;Oh, Yoon-Sik;Kim, Hyung-Seop;Lee, In-Kyu
    • ALGAE
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    • v.19 no.2
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    • pp.79-90
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    • 2004
  • Nuclear DNA contents of spermatia from eight ceramiacean and four dasyacean algae (Ceramiales, Rhodophyta) and microspores from two land plants were estimated by fluorescence microscopic image processing and their nuclear SSU rDNA sequence data were analyzed. In frequency distribution patterns, the DAPI-stained nuclear volume (NV) of spermatia showed two peaks corresponding to 1C and 2C. Nuclear 2C DNA contents estimated from NV were 0.45-2.31 pg in ceramiacean and 0.40-0.57 pg in dasyacean algae and 8.42-9.51 pg in two land plants, Capsicum annuum and Nicotiana tabacum. By nuclear patterning of vegetative cells derived from an apical cell, 2C DNA contents of spermatia were 2.31 pg in an alga having uninucleate and non-polyploid nucleus (Aglaothamnion callophyllidicola), 0.45-1.94 pg in algae having uninucleate and polyploid nucleus (Antithamnion spp. and Pterothamnion yezoense), and 0.40-0.62 pg in algae having multinucleate and non-polyploid nuclei (Griffithsia japonica and dasyacean algae). Each mature spermatium and microspore (pollen grain) seemed to have a 2C nucleus, which may provide a genetic buffering system to protect the genetic content of a spermatium and microspore from potentially lethal mutations. Nuclear DNA content and SSU rDNA sequence of Antithamnion sparsum from Korea were reasonably different from those of Antithamnion densum from France. The data did not support the previous taxonomic studies that these two taxa could be conspecific.

Characteristics of Lactobacillus reuterii BSA-131 Isolated from Swine Intestine (가축의 장관에서 분리한 Lactobacillus reulterii BSA-131의 특성)

  • Chang, Young-Hyo;Kim, Jong-Keun;Kim, Hong-Joong;Yoon, Jung-Hoon;Kim, Won-Yong;Choi, Yang-Woong;Lee, Won-Joon;Kim, Young-Bae;Park, Yong-Ha
    • Microbiology and Biotechnology Letters
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    • v.27 no.1
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    • pp.23-27
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    • 1999
  • The development and industrial application of intestinal anaerbic bacteria is highly important in the munufacture of foodstuffs and medical products. We have conducted studies, screening anaerobic intestinal bacteria for acid and bile tolerance and for pathogens suppression in the intestine of swine. One such study was taxonomic in nature and dealt with the isolate's biochemical responses. The results indicated that the BSA-131 strian ioslated. which suppressed various pathogens (13 typical strains), were identified as Lactobacillus reuterii. The strain, which named L. reuterii BSA-131, was able to tolerate acid condition down to pH2 and could also tolerate bile at 5%. The strain exhibited resistance to a range of antibiotics including, cephalexin, erythromycin, flumequine, furazolidine, gentamycin, penicillin G, norfloxacin, spectinomycin, tetracycline, tiamuline, neomycin, chloramphenicol, kanamycin etc.

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New Report of Three Xanthid Crabs (Crustacea: Decapoda: Xanthidae) from Korea

  • Lee, Sang-Kyu;Shin, Myung-Hwa;Park, Tae-Seo;Kim, Won
    • Animal Systematics, Evolution and Diversity
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    • v.28 no.2
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    • pp.117-125
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    • 2012
  • Many members of xanthid crabs have the black coloured fingers and are found easily in the intertidal or subtidal zone. Three xanthids, $Danielea$ $noelensis$ (Ward, 1934), $Etisus$ $anaglyptus$ H. Milne Edwards, 1834, and $Gaillardiellus$ $rueppelli$ (Krauss, 1843), are newly reported from Korean waters as a result of continuous taxonomic studies on crabs. Of these, the present specimen of $D.$ $noelensis$ has rather projecting angle on the antero-external border of the merus of the third maxilliped differed from that of the original description. The genus $Danielea$ Ng and Clark, 2003 and the genus $Etisus$ H. Milne Edwards, 1834 are also reported first time from Korean fauna. Their descriptions are provided with illustrations. Korean Xanthoidea now consists of 28 species belonging to 24 genera.