• Radiation Oncology Journal
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• v.12 no.3
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• pp.295-300
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• 1994

It was the purpose of present study to develop a new thermoseed for heating deep-seated tumors and assessment of the effect of magnetic control on thermoseeds. Aqueous suspension of iron micro spheres (Ferropolysaccharide) was injected directly into the VX-2 hepatoma and heated with 1.2 MHz inductive radiofrequency unit. Aqueous thermoseed suspension was delivered to the tumor by simple percutaneous injection. The limitation of the thermoseed heating method is the positional change of thermoseed particles in the tumor after implantation. The thermoseed particles could enter the systemic blood circulation and cause a severe embolization of a critical organ. To minimize this limitation, we have used the magnetic control after loading the thermoseed in the tumor, W hen ferropolysaccharides were exposed to a strong magnetic field, they magnetized and subsequently exerted a magnetic force on each other, forming larger aggregates of particles. The size of aggregated Particles were too big to enter the systemic blood circulation. Thus, unlike other thermoseed method, we hold the thermoseed particles stationary in the tumor. The temperature of the injected site and immediate vicinity elevated by $4-5^{\circ}C$. The temperature of the surrounding normal hepatic tissue elevated by $1-2^{circ}C$ only. The heating effect within the tumor was variable depending on the density of ferromagnetic aqueous suspension. Our results suggest that inductive heating of tumor injected with ferropolysaccharide solution offers the possibility of effective heat delivery to the defined tumor volume, which is difficult to heat with other heating devices.

• Journal of Biomedical Engineering Research
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• v.23 no.3
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• pp.189-196
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• 2002

Transdermal drug delivery offers an alternative method to the conventional oral and injection delivery method. Its advantages include its ability to deliver drugs directly into systemic circulation. However, there have been restrictions in its application to deliver drugs because of the skin's barrier function. In this study, we try to combine a Sonophoresis and oxygen Pressure method in order to increase the Permeability of the skin. we used water as the compound and by utilizing the skin impedance method. we measured the hydration Permeability of skin Ultrasound was applied using a sonicator(Solcare-U1000. Solco, Korea) operating at a frequency of 1MHz. oxygen Pressure was applied using a compressor(Oxyjet-Pointer, Nora Bode. Germany) operating at a pressure of 2Bar/cm2. Experiment was performed in vivo for 42 People. We divided the subjects into four smaller groups. A different transdermal drug delivery method was applied for each group on the back of their hand. We measured the skin impedance variations on the hand. during a 20-minute time Period. The control group did not show any significant increase or variation of skin impedance to water. In comparison to the control group(Passive diffusion) the hydration Permeability of the ultrasound group and the oxygen Pressure group was approximately 25 and 30 times higher consecutively. Futhermore, the hydration permeability of the combination of ultrasound and oxygen Pressure group was about 70-fold higher in comparison to the control group(passive diffusion) . The results reveal that a combination of ultrasound and oxygen Pressure will significantly enhance transdermal water transport compared when only one of them is used.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• v.25 no.4
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• pp.304-310
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• 1999

This study was designed to find the effect of Minocycline loaded microcapsule applied locally to tissue by measuring drug concentration in tissue and serum by HPLC and to achieve optimal drug delivery system and duration to a specific target site. Control group were administrated minocycline intramuscularly twice a day with $0.2{\mu}g/100g$ for 1 to 10 days. In experimental group, surgical wound was created on Rt. cheek and then minocycline loaded microcapsule was applied into the space between superficial and deep layer of masseter muscle. Animals were sacrificed at 1, 3, 5, 7, 10 days after initial administration, blood was obtained from heart and right masseter muscle was excised. Blood sample was centrifuged at 3000rpm for 15min. Tissue sample was homogenized, left at room temperature for 48hr and centrifuged at 4000g for 5min. Supernatant was completely dried and dissolved in distilled water. Analysis was conducted using a ${\mu}Bondapack$ C18 column. The mobile phase was 0.2M Ammonium Oxalate/0.1M EDTA/DMF=11/4/5 solution, which was injected into the column and detected with photodiode detector at 344nm wavelength. The results were as follows : 1. This method was reliable, could be replicated and suitable for minocycline analysis in tissue as well as serum. 2. In tissue, concentration of minocycline of experimental group was higher than that of control group for 5days. 3. Except 1 day, concentration of minocycline in serum of experimental group was lower than that of control group. 4. Concentration of minocycline in tissue was much higher than that in serum. From these results, minocycline loaded microcapsule might be effective tool for local drug delivery system might be useful for treatment of infections of oral and maxillofacial region and management of infected surgical wound, minimizing systemic effects.

• Journal of Microbiology and Biotechnology
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• v.19 no.3
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• pp.307-313
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• 2009

In order to systemically investigate the possibility of using coxsackievirus B3 (CVB3) to deliver foreign genes in vivo, a recombinant strain of CVB3 encoding the renilla gene (CVB3-renilla) was constructed. The recombinant CVB3 resulted in extensive and transient expression of the renilla protein within mouse organs, especially the pancreas. The level of expression was generally dependent upon the viral titer present. Moreover, the CVB3-renilla strain was completely attenuated. Interestingly, the recombinant CVB3 vector was expressed much more strongly in mouse organs than was a comparable adenoviral vector. The CVB3-renilla strain did not express the renilla gene in mice with pre-existing coxsackievirus-specific neutralizing antibodies, but direct organ-specific administration of the virus during open-peritoneum surgery was able to circumvent this immunity. This coxsackievirus vector may represent a useful means for delivering and expressing foreign genes in mouse models in an acute and extensive fashion.

• Journal of Periodontal and Implant Science
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• v.24 no.3
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• pp.529-540
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• 1994

Rapidly progressive periodontitis is known to be usually associated with systemic problems and improved with antibiotic therapy. Recent experiments in which bioresorbable polycaprolactone was polymerized with minocycline has shown that the system released effective antibiotic concentration during the 7 days' period. This clinical trial was to compare the efficacy of a minocycline film(poly-caprolactone+polyglycol+10% minocycline) insertion plus supragingival scaling(MS) or subgingival scaling & root planing(MSRP) with the scaling(S) or subgingival scaling & root planing alone(SRP), at improving the periodontal condition in RPP. Fifteen patients were examined for plaque accumulation, gingival inflammation, probing depth and attachment loss at baseline, then 1, 2, 4 and 8 weeks after 4 treatment regimens were randomly undergone in 4 comparable sites(PD>5mm, LA>3mm) in each subject. Results revealed statistically significant treatement effect with a reduction in a probing depth in SRP(2.0mm), MS(1.8mm), and MSRP(2.1mm). There was no significant reduction in the supragingival scaling alone group(0.6mm). Similarly, attachment levels were significantly improved in the SRP(1.5mm), MS(2.0mm) and MSRP(2.0mm) groups. Net % BOP reduction at 8 week compared to baseline was 6.7% (S), 26.7% (MS), 26.7% (SRP), and 33.3% (MSRP). MSRP produced the greatest improvement in BOP at 8 week. This data suggests that a subgingival minocycline delivery system as a adjunct to scaling alone or scaling & root planing may produce significant clinical benefits over scaling alone in rapidly progressive periodontitis patients.

• Korean Journal of Occupational Health Nursing
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• v.30 no.4
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• pp.186-195
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• 2021

Purpose: The current status and rationale of industrial accidents needs to be examined to develop scientific and systemic preventive measures. Methods: The aim of this study is to analyze the current data on industrial accidents provided by the Ministry of Employment and Labor and categorize work-related deaths by types of industries and annual report. Results: First, the highest number of deaths occurred in industries that had less than 50 people. Second, in the manufacturing industry, the highest death rate was found in workers in the age group 50-59 years. In the construction industry, workers aged 50 and above had the highest number of deaths. In other industries, workers aged 60 and above had the highest number of deaths. Third, the highest number of deaths occurred in workers with less than one year of experience in any industry Fourth, in most industries, the highest work-related deaths occurred during weekdays (Monday~Friday). In 2015, the warehouse delivery industry had 33% higher work-related deaths on the weekends (Saturday and Sunday) as compared to other industries. Fifth, in most industries, the highest work-related deaths occurred from 8 AM to 6 PM. The warehouse delivery industry had higher work-related deaths from 10 PM to 8 AM as compared to other industries. Conclusion: In order to increase the efficacy of industrial accident prevention, it is necessary to establish an effective health management system and apply strict safety management activities.

• The Journal of Korean Obstetrics and Gynecology
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• v.22 no.4
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• pp.173-186
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• 2009

Purpose: 1. To analyze the satisfaction and cognition of postpartum care and to aid the spread of oriental medicine in postpartum care. 2. Get a basic guideline of O.M.D's postpartum care. Methods: We studied the satisfaction and cognition of postpartum care with questionnaire from January 2009 to August 2009, who was treated in ○○ Oriental Medical Postpartum care center during 2 weeks. 61 puerperants answered us. Results: 1. In choosing Postpartum care center, puerperants concerned about new born infants' care(50.8%) and treatment for discomfort after delivery(27.86%). 2. 22 puerperants(36.6%) had a positive view in folk remedy for postpartum care and 34 puerperants(55.73%) thought it would be good to eliminate edema and loose weight. 3. Most of puerperants(80.32%) chose oriental medical postpartum care because they wanted to receive oriental medical treatment. 4. 5 puerperants(8.19%) were very satisfied with oriental medical postpartum care than other type of postparum care which they experienced at first delivery. 47(77.04%) were satisfied, 2(3.27%) were unsatisfied and no one was very satisfied. 5. To the questionnaire about the effect of strength recovery, lochia excretion, edema limination, increasing amount of breastmilk, reducing arthralgia, about 36.8% puerperants showed positive view. 6. The positive view about Acupuncture, cupping, moxibustion, herbal treatment was, respectively, 90.16%, 49.18%, 73.77%, 86.88%. Conclusion: Puerperants were satisfied with oriental medical postpartum care and had positive cognition in oriental medical treatment. There is much capability for extension of oriental medicine in postpartum care. So continuous concern, systemic development and publicity about oriental medical postpartum care are required.

• The Journal of Korean Academy of Prosthodontics
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• v.51 no.4
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• pp.332-338
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• 2013

Data collection including checking the problem list and analyzing the etiology cannot be overemphasized in full mouth rehabilitation cases. It is well reported that favorable prognosis of prosthodontic treatment depends on accurate diagnosis. Seventy seven year old female patient presented with chief complaints of chewing pain on maxillary and mandibular left premolars and unesthetic existing prosthesis. Clinical evaluation, radiographic examination and cast analysis were performed to gather comprehensive data and problem lists. Treatment planning was based on collected data and related articles. Abutment evaluation, prognosis depending on various types of prosthesis, and maintenance were considered as well. Occlusal vertical dimension was increased to solve esthetic dissatisfaction and insufficient interocclusal space. Patient adaptation was verified with provisional restorations followed by diagnostic wax up. Function, esthetics and occlusal stability were verified during 8 week follow-up period. Prosthodontic reconstruction based on systemic analysis, diagnosis, and treatment plan led to satisfactory result after delivery of definitive prosthesis.

• Korean Journal of Veterinary Research
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• v.53 no.4
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• pp.199-205
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• 2013

Avian pathogenic Escherichia coli (APEC) is a causative agent for a number of extra intestinal diseases and account for significant losses to the poultry industry. Since protective immunity against APEC is largely directed to virulence antigens, we have individually expressed four different viulence antigens, papA, papG, IutA, and CS31A, using an attenuated Salmonella Typhimurium and a plasmid pBB244. Following oral immunization of mice with combination of two or four of these strains, serum IgG and mucosal IgA responses were elicited against each antigen represented in the mixture. The antigen-specific mucosal IgA responses were significantly higher in the group of mice immunized with the heat-labile Escherichia coli enterotoxin B subunit (LTB) strain than those in the group of mice immunized without the LTB strain. While, there was no significant difference between these two groups in antigen-specific serum IgG responses. The results showed that LTB could act as mucosal immune adjuvant. To assess the nature of immunity, the distribution of antigen-specific IgG isotypes was analyzed. All groups promoted Th1-type immunity as determined by the IgG2a/IgG1 ratio. Thus, our findings provided evidence that immunization with a combination of several vaccine strains is one of the strategies of developing effective vaccines against APEC.

• Journal of Pharmaceutical Investigation
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• v.24 no.1
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• pp.1-9
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• 1994

In order to study systemic peptide delivery through the ocular route, the stabilities of methionine enkephalin (Met-Enk) and $[D-ala^2]-methionine$ enkephalinamide (YAGFM) in the corneal extracts of rabbits were investigated using reversed phase HPLC. Met-Enk was found to be hydrolyzed most rapidly in the corneal epithelium, but YAGFM was relatively stable. Aminopeptidases appeared to contribute over 60% to the degradation of Met-Enk and the degradation rate of Met-Enk followed the first order kinetics. The half-lives of Met-Enk in the extracts of the corneal epithelium and endothelium were 36 and 673 min, respectively. From the effects of enzyme inhibitors, it was found that the application of the mixture of amastatin, thimerosal and EDTA was very useful for the inhibition of peptide degradation.