• 한국약용작물학회지
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• 제22권3호
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• pp.196-202
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• 2014

Depression is one of the most common neuropsychiatric disorders and has been associated with the neuroendocrine system and alterations in behavior. Schisandra chinensis Baillon is one of major medicinal plants used as a Korea medicine and food sources, and has been processed in the fields of various food products and medicinal herbs. The chronic mild stress (CMS) protocol is widely used to evoke depressive-like behaviors in laboratory mice or rat. The CMS procedure induced some behavioral changes that are compatible with the common expectations, i.e. 'anhedonic' behavior and can affect corticosterone level. The present study, Schisandra chinensis extract administration by daily gavage from the 3 weeks exhibited an antidepressant-like effect on CMS-induced depression in mice. Schisandra chinensis extract administration at dose of 200mg/kg significantly increased the sucrose consumption, and decreased the immobility durations in forced swim test and tail suspension test. Furthermore the corticosterone level decreased than control group. In conclusion, Schisandra chinensis extract showed antidepressant-like effects on sucrose preference test, forced swimming test and tail suspension test based on CMS model.

• 대한본초학회지
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• 제31권3호
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• pp.49-57
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• 2016

Objectives： Polygonati Rhizoma (PR) has containing the bioactive compounds such as poly sccharide A,B,C, oligosaccharide, amino acid, it has reported to anti-diabetes and hypertension, atherosclerosis. In this study, we were evaluates antioxidant and anti-physical fatigue effects of PR and steamed PR.Methods : The sample was divided into 5 groups-PR0 (PR without steaming process), PR1 (PR with once steaming process), PR3 (PR with third steaming process), PR6 (PR with sixth steaming process), PR9 (PR with ninth steaming process). We measured anti-oxidant activity through contents of polyphenol, flavonoid and DPPH, ABTS free radical scavenging capacity. And, anti-physical fatigue effect was evaluated using the swimming test, and the AMPK protein expressions in soleus muscle.Results ： As a result, polyphenol, flavonoid, DPPH, ABTS free radical scavenging capacity of PR were increased as steaming times. Anti-physical fatigue effects by swimming test, PR0 have significantly increased, but steamed PR groups were decreased. The AMPK protein expressions of PR0 and PR1 groups were increased comparing with PR3, PR6 and PR9. All groups had effects on decreasing TG, creatine in blood serum, but had no effects on TC in blood serum.Conclusions ： In conclusion, PR with 9 steaming process was more excellent than not-processed PR in anti-oxidant effect such as DPPH, ABTS radical scavenging activity and contents of polyphenol, flavonoid, but, not-processed PR increased swimming times than processed PR. These results suggest that processed PR has anti-oxidant effect as steaming times, and not-processed PR may be a novel potential anti-physical fatigue agents than processed PR.

• Journal of Ginseng Research
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• 제24권4호
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• pp.202-205
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• 2000

계지인삼탕중 고려인삼을 보기조양 효능이 더 강한 고려홍삼으로 바꾼 계지홍삼탕을 시료로 항산화작용과 지구력효과를 비교검토 하였다. 항산화작용중 AAPH에 의한 적혈구 용혈작용은 고려홍삼과 계지홍삼탕이 유효하게 억제되었으나,DPPH에 대한 유리산소 소거율은 BHT보다는 약하였지만 농도의존적으로 유의성 있게 감소되었고, in vivo에서 hAPn처리후 시간경과(1시간, 3시간, 6시간)에 따른 MDA제거율도두 실험군에서 대조군에 비해 시간과 농도에 비례하여 유의하게 감소하였지만 6시간후에는 두 실험군간에 통계적 편차는 없었다. 수영법에 의한 지구력 측정에서는 두 실험군이 대조군에 비해 유의한 증가를 보였지만 시료 투여후 2주에는 고려홍삼이 보다 유효했고, 4주째는 계지홍삼탕이 저농도(25mg/rg)에서만 유효했다. 이상의 결과로 보아 고려삼과계지홍삼탕은 항산화 및 지구력 증진 효과가 있는 것으로 사료되지만 지속적인 보완 연구가 필요하다고 사료된다.

• 한국식품과학회지
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• 제11권1호
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• pp.50-55
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• 1979

인삼 nectar, 인삼추출물이 함유된 orange juice, 인삼 drink 제등을 정상식이에 첨가 (555 mg Ginseng extract/kg of diet, 1,110 mg Ginseng extract/kg diet)하여 이유 직후의 Sprgue-Dawley strain 백쥐 40마리를 8마리씩 5군으로 나누어 12주간 사육하였다 12주후에 체중증가율, 사료효율, 장기의 무게, hematocrit value, serum cholesterol content, serum GOT and GPT 활성등을 측정하여 정상식이군과 비교한 결과 체중증가율, 사료효율, 장기의 무게, hematocrit value, SGOT 활성등을 모두 정상식이군에 비해서 실험군이 통계적으로 유의차를 보이지 않았으나, s-GOT 활성은 대조군에 비해 실험군이 다소 낮은 경향을 관찰할 수 있었고, s-cholesterol 농도는 대조군에 비해 다소 높은 경향이었으나 통계적인 유의차는 없었다. 백쥐 각군의 간, 신장, 비장등의 조직세포를 검사한 바 하등의 이상 조직을 발견하지 못하였다. 12주 실험이 끝난 후 체중을 측정하고 swimming test를 실시한 결과 대조군에 비해 실험군의 수영시간이 연장되었으며 V군에서는 유의성있게 연장되었다.

• 대한본초학회지
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• 제30권3호
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• pp.13-17
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• 2015

Objectives : Taraxacum platycarpum H. Dahlstedt has been reported to have several biological properties such as skin hydration and antiinflammation. The purpose of this study was to examine the antidepressive effects of water extract of T. platycarpum (WTP) on an animal model of depression. Methods : In the present study, normal ICR mice (4 weeks) were used, and orally administered with WTP (25, 50 and 100 mg/kg). Depression-like behavior was monitored the forced swimming test (FST) and tail suspension test (TST) in mice. The locomotor activity was evaluated to eliminate the false-positive activity in the open field test (OFT). Fluoxetine, the selective serotonin reuptake inhibitor, as a positive control was intraperitoneally administered at a dose of 15 mg/kg at 30 min before starting the behavioral test. Moreover, we evaluated the effects of WTP on the expression of brain-derived neurotrophic factor (BDNF) and the extracellular signal-regulated kinase (ERK)/ cyclic AMP response-element binding protein (CREB) signaling pathway in the hippocampus using Western blot. Results : The administration of WTP (50 and 100 mg/kg) significantly (P < 0.05, respectively) reduced the immobility time during FST and TST without accompanying changes in locomotor activity by OFT. Furthermore, WTP at dose of 100 mg/kg increased the BDNF expression and the phosphorylation of ERK and CREB in the hippocampus region. Conclusions : These results suggest that WTP has a useful anti-depressant effect through the regulation of BDNF/ERK/CREB signaling pathway.

• 대한한의학회지
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• 제21권2호
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• pp.68-78
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• 2000

Objective : This research was performed to investigate protective effects of Sophora subprostrata, against ischemic brain damage after a middle cerebral artery(MCA) occlusion. The effect was estimated using histological test, neurobehavioural test, and biochemical test. Methods : Rats(Sprague-Dawley) were divided into four groups: Sham operated group, MCA occluded group, Sophora subprostrata administrated group after MCA occlusion, and Normal group. The MCA was occluded by intraluminal method. Sophora subprostrata was administrated orally twice(l and 4 hours) after middle cerebral artery occlusion. The neurobeavioural test was performed at 3 hours, 6 hours, 9 hours and 24 hours after the surgery by posture reflex test and swimming behavioural test. All groups were sacrificed at 24 hours after the surgery. The brain tissue was stained with 2% triphenyl tetrazolium chioride(TTC) or 1 % cresyl violet solution, to examine effect of Sophora subprostrata on ischemic brain tissue. The blood samples were obtained from the heart of rats. Tumor necrosis factor-a level was measured from sera using Enzyme-Linked Immunoabsorbent Assay(ELISA). Results : The results showed that (1) Sophora subprostrata reduced infarct size and total infarct volume by 54.8% compared to the control group, (2) that neuronal death, which was shown by decrease in cell number and size, was attenuated significantly in the boundary area of the infarction, (3) that serum $TNF-{\alpha}$ㆍlevel was reduced significantly, and finally, there was significant recovery of motor deficit at 3 hours after MCA occluded by Swimming behavioural test. Conclusions :In conclusion, Sophora subprostrata has protective effects against ischemic brain damage at the early stage of ischemia.

• 한국생명과학회:학술대회논문집
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• 한국생명과학회 2008년도 제49회 학술심포지움 및 국제학술대회
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• pp.37.2-37.2
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• 2008

• Journal of Pharmaceutical Investigation
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• 제14권4호
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• pp.178-182
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• 1984

Extract of drugs in commerce was administered to mice for 3 days and 7 days and its antifatigue effects were compared with those of P. ginseng extract and A. sessiliflorum extract. Mice were used for these experiments divided into ethyl alcohol and water extract of R. glutinosa(saengjihwang), R. glutinosa (sookjihwang), A. japonica(changchool), A. japonica(baekchool), P. officinale, C. japonica, C. morifolium, A. calamus were given ordinary dose rally. For antifatigue test, swimming performance were determined after the extract administration. The group of P. officinale gave high improvement of the swimming performance in ethyl alcohol extract group.

• Journal of Pharmaceutical Investigation
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• 제14권4호
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• pp.195-199
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• 1984

Extract of drugs in commerce was administered to mice for 3 days and its fatigue effects were compared with those of P. ginseng extract and A. sessiliflorum extract. Mice were used for these experiments divided into ethyl alcohol and water extract of P. cocos, S. japonica, P. multiflorum, L. chinense, A. cochinchinensis, S. indicum, N. nucifera were given ordinary dose orally. For antifatigue test, swimming performance were determined after administration of the extract The group of P. cocos gave high improvement of the swimming performance in ethyl alcohol extract group. And the group of S. japonica an improvement was also observed in water extract group.

• 동의신경정신과학회지
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• 제18권1호
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• pp.1-14
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• 2007

Objective : The purpose of this study was to investigate the protective effects of Cheongsimondam-tang(COT) on the animal model of depression induced immobilization stress. Method 1) Male rats were used for this experiment. The subject were divided into 4 groups(1. normal 2. saline solution administered during immobilization stress treatment 3. COT of 100mg/kg administered 4. COT of 400mg/kg administered). 2) Immobilization stress was treated for 1 hours on day. During 2 days of immobilization stress treatment, they were executed forced swimming test, passive avoidance test, elevated plus maze test. Corticosterone in blood were measured Results 1) In EPM test, stress group showed significantly increased anxiety, COT groups showed significantly decreased anxiety. 2) In forced swimming test, COT groups did not show significantly decreased immobilization. 3) In passive avoidance test, stress group showed significantly decreased learning execution, COT groups showed significantly increased learning execution. 4) Stress group showed significantly increase in serum level of corticosterone, COT of 400mg/kg group showed significantly decreased serum level of corticosterone. Conclusion : These results suggest that Cheongsimondam-tang(COT) is effective in the treatment of depression.