• Title/Summary/Keyword: swelling strain

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Oral tissue response to soft tissue expanders prior to bone augmentation: in vitro analysis and histological study in dogs

  • Yoo, Jung Min;Amara, Heithem Ben;Kim, Min Kyoung;Song, Ju Dong;Koo, Ki-Tae
    • Journal of Periodontal and Implant Science
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    • v.48 no.3
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    • pp.152-163
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    • 2018
  • Purpose: To determine whether the swelling and mechanical properties of osmotic self-inflating expanders allow or not the induction of intraoral soft tissue expansion in dogs. Methods: Three different volumes (0.15, 0.25, and 0.42 mL; referred to respectively as the S, M, and L groups) of soft tissue expanders (STEs) consisting of a hydrogel core coated with a silicone-perforated membrane were investigated in vitro to assess their swelling behavior (volume swelling ratio) and mechanical properties (tensile strength, tensile strain). For in vivo investigations, the STEs were subperiosteally inserted for 4 weeks in dogs (n=5). Soft tissue expansion was clinically monitored. Histological analyses included the examination of alveolar bone underneath the expanders and thickness measurements of the surrounding fibrous capsule. Results: The volume swelling ratio of all STEs did not exceed 5.2. In tensile mode, the highest mean strain was registered for the L group ($98.03{\pm}0.3g/cm$), whereas the lowest mean value was obtained in the S group ($81.3{\pm}0.1g/cm$), which was a statistically significant difference (P<0.05). In addition, the S and L groups were significantly different in terms of tensile strength ($1.5{\pm}0.1g/cm$ for the S group and $2.2{\pm}0.1g/cm$ for the L group, P<0.05). Clinical monitoring showed successful dilatation of the soft tissues without signs of inflammation up to 28 days. The STEs remained volumetrically stable, with a mean diameter in vivo of 6.98 mm, close to the in vitro post-expansion findings (6.69 mm). Significant histological effects included highly vascularized collagen-rich fibrous encapsulation of the STEs, with a mean thickness of $0.67{\pm}0.12mm$. The bone reaction consisted of resorption underneath the STEs, while apposition was observed at their edges. Conclusions: The swelling and mechanical properties of the STEs enabled clinically successful soft tissue expansion. A tissue reaction consisting of fibrous capsule formation and bone loss were the main histological events.

Isolation and identification of cellulolytic Actinomycetes (Cellulose 분해 방선균의 분리 및 동정)

  • 정현호;성하진;최용진;양한철
    • Microbiology and Biotechnology Letters
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    • v.14 no.5
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    • pp.377-383
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    • 1986
  • About 300 cellulolytic actinomycetes isolated from soils were tested for their cellulase activities estimated by means of filter paper swelling and carboxymethyl cellulose saccharifying activity. Then, 16 isolates which had shown relatively high levels of CMCase activity were selected and examined for their abilities of $\beta$-glucosidase production. Among them strain No. 109 was found to have highest level of intracellular $\beta$-glucosidase, and selected for the further studies. In this paper, the cultural, morphological and physiological properties, and cell wall composition of strain No. 109 were described in relation to the taxonomic status of this actinomycete. Based on the results obtained in these experiments strain No. 109 was identified to be a similar species to Streptomyces tanashiensis.

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Histopathological Studies on Effect of Dropwort on Cadmium Poisoning in Mice (미나리가 마우스의 카드뮴 중독에 미치는 병리조직학적 관찰)

  • 장종식
    • Journal of Veterinary Clinics
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    • v.16 no.1
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    • pp.13-18
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    • 1999
  • This study was undertaken to find out the effect of dropwort on histopathological changes of cadmium toxicity in mice. Thirty two mice of BALB/c strain were divided into a control group (A) and three experimental groups (B, C, D). Each group was consisted of four males and four females : group A received tap water and basal diet, group B received tap water and diet supplemented with 100 mg/kg of cadmium, group C received dropwort juice and diet supplemented with 100 mg/kg of cadmium, group D received dropwort juice and basal diet. All mice were dissected on the 35th day. Pathological changes in liver, kidney, spleen, heart, lung, stomach and muscle were observed. Group D showed no significant change as the control group. But group B showed many cytoplasmic vacuoles and necrosis of hepatocytes, glomerular swelling, and degeneration and necrosis of convoluted tubules in kidney, marked congestion and hemorrhage, and a large number of variably sized alveolar macrophages appearance in lung and swelling in some part of muscle fiber. On the other hand, group C showed a little convalescent changes and maintained their normal architectures in liver, kidney, lung and muscle.

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A tunable inverse-hemisphere-shaped Bragg grating sensor (튜닝가능한 역반구형의 브래그 그레이팅 센서)

  • Ryu, Yunha;Kim, Kyoungsik
    • Transactions of the Society of Information Storage Systems
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    • v.9 no.2
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    • pp.48-50
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    • 2013
  • In this work, we investigated the diffraction of inverse-hemisphere shaped polymer grating. The grating was fabricated by using soft lithography of hexagonally close-packed PS nanospheres. The periodicity of the grating was tuned by swelling in acetone and the diffraction wavelength shift induced from lattice change was measured. This device can be used as a strain gauge or a chemical sensor.

Fission accelerated steady-state post irradiation examinations - Part II

  • Sobhan Patnaik;Geoffrey L. Beausoleil II;Luca Capriotti
    • Nuclear Engineering and Technology
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    • v.56 no.10
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    • pp.4158-4168
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    • 2024
  • The Advanced Fuels Campaign's Fission Accelerated Steady State Testing (FAST) approach at Idaho National Laboratory creates a benchmark for evaluating accelerated irradiation via control rodlets and advanced metal fuel alloys for sodium-cooled fast reactors (SFRs). FAST experiments have been developed to generate prototypic temperature conditions during steady state irradiations of scaled geometric fuel pins. This approach helps to attain higher burn ups at a much faster rate than previous irradiation tests. For this study, the results from profilometry, fission gas release, and metallography of a FAST experiment are presented. Profilometry determined 0 % effective strain in the rodlets. The fission gas release fraction was measured from puncture/collection analysis. Constituent redistribution was observed in two specimens despite the peak fuel temperatures being below the normal ranges in which redistribution is expected. Metallography of the two higher temperature specimens showed typical swelling with the solid pin closing the fuel-cladding gap and the annular specimen having a fully closed annulus. Additionally, metallography indicated no swelling, no redistribution, and a homogenous microstructure for specimens with lower irradiation temperature. Post irradiation examination of FAST rodlets generally showed the expected representative behavior of metallic fuels within SFRs.

Isolation of Bacterial Strain Antagonistic to Pyricularia oryzae and Its Mode of Antifungal Action

  • Bae, Dong-Won;Lee, Joon-Taek;Son, Dae-Young;Lee, Eun Sook;Kim, Hee-Kyu
    • Journal of Microbiology and Biotechnology
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    • v.10 no.6
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    • pp.811-816
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    • 2000
  • An antagonistic bacterium PM-1 which strongly inhyibits the growth of Pyricularia oryzae was isolated and identified as paenibacillus macerans. The antifungal substances of the strain PM-1 showed the broad antifungal spectra against P.oryzae races. Relating to the localization test, it was found that the antifungal substances existed not only in the cytoplasm but also in the culture supernatant, and importantly the antifungal activity of the latter was stronger than that of the former. The extracellular antifungal substances were extremely heat-stable up to $121^{\circ}C$ for 15 min. The substances were optimally produced at $20^{\circ}C$ and pH 10.0 in a potato dextrose broth. The culture filtrate of the strain PM-1 caused a partial swelling of the mycelia of P.oryzae, and it prevents the normal growth of the fungus as well. This result suggested that the antifungal substances secreted by the strain PM-1 potentially inhibited the germination of P.oryzae.

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Antifungical Activity of Autochthonous Bacillus subtilis Isolated from Prosopis juliflora against Phytopathogenic Fungi

  • Abdelmoteleb, Ali;Troncoso-Rojas, Rosalba;Gonzalez-Soto, Tania;Gonzalez-Mendoza, Daniel
    • Mycobiology
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    • v.45 no.4
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    • pp.385-391
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    • 2017
  • The ability of Bacillus subtilis, strain ALICA to produce three mycolytic enzymes (chitinase, ${\beta}$-1,3-glucanase, and protease), was carried out by the chemical standard methods. Bacillus subtilis ALICA was screened based on their antifungal activity in dual plate assay and cell-free culture filtrate (25%) against five different phytopathogenic fungi Alternaria alternata, Macrophomina sp., Colletotrichum gloeosporioides, Botrytis cinerea, and Sclerotium rolfesii. The B. subtilis ALICA detected positive for chitinase, ${\beta}$-1,3-glucanase and protease enzymes. Fungal growth inhibition by both strain ALICA and its cell-free culture filtrate ranged from 51.36% to 86.3% and 38.43% to 68.6%, respectively. Moreover, hyphal morphological changes like damage, broken, swelling, distortions abnormal morphology were observed. Genes expression of protease, ${\beta}$-1,3-glucanase, and lipopeptides (subtilosin and subtilisin) were confirmed their presence in the supernatant of strain ALICA. Our findings indicated that strain ALICA provided a broad spectrum of antifungal activities against various phytopathogenic fungi and may be a potential effective alternative to chemical fungicides.

High Deformable Concrete (HDC) element: An experimental and numerical study

  • Kesejini, Yasser Alilou;Bahramifar, Amir;Afshin, Hassan;Tabrizi, Mehrdad Emami
    • Advances in concrete construction
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    • v.11 no.5
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    • pp.357-365
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    • 2021
  • High deformable concrete (HDC) elements have compressive strength rates equal to conventional concrete and have got a high compressive strain at about 20% to 50%. These types of concrete elements as prefabricated parts have an abundance of applications in the construction industry which is the most used in the construction of tunnels in squeezing grounds, tunnel passwords from fault zones or swelling soils as soft supports. HDC elements after reaching to compressive yield stress, in nonlinear behavior have hardening combined with increasing strain and compressive strength. The main aim of this laboratory and numerical research is to construct concrete elements with the above properties so the compressive stress-strain behavior of different concrete elements with four categories of mix designs have been discussed and finally one of them has been defined as HDC element mix design. Furthermore, two columns with and without implementing of HDC elements have been made and stress-strain curves of them have been investigated experimentally. An analysis model is presented for columns using finite element method adopted by ABAQUS. The results obtained from the ABAQUS finite element method are compared with experimental data. The main comparison is made for stress-strain curve. The stress-strain curves from the finite element method agree well with experimental results. The results show that the dimension of the HDC samples is significant in the stress-strain behavior. The use of the element greatly increases energy absorption and ductility.

Isolation and Characterization of Bacillus sp. Producing Broad-Spectrum Antibiotics Against Human and Plant Pathogenic Fungi

  • Chen, Na;Jin, Min;Qu, Hong-Mei;Chen, Zhi-Qiang;Chen, Zhao-Li;Qiu, Zhi-Gang;Wang, Xin-Wei;Li, Jun-Wen
    • Journal of Microbiology and Biotechnology
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    • v.22 no.2
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    • pp.256-263
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    • 2012
  • A strain of bacterium producing antifungal antibiotic was isolated and identification of the strain was attempted. We could identify the bacterium as being a Bacillus sp., based on morphological observation, physiological characteristics, and 16S rDNA sequence analysis, thus leading us to designate the strain as Bacillus sp. AH-E-1. The strain showed potent antibiotic activity against phytopathogenic and human pathogenic fungi by inducing mycelial distortion and swelling and inhibiting spore germination. The antibiotic metabolite produced by the strain demonstrated excellent thermal and pH (2-11) stability, but was labile to autoclaving. From these results, we could find a broader antifungal activity of Bacillus genus. Isolation and characterization of the active agent produced by the strain are under progress.

Isolation and Characterization of Burkholderia cepacia strain YJK2, Antagonistic Microorganism of Paprika Pathogens (파프리카 병원균들에 대한 길항미생물, Burkholderia cepacia strain YJK2의 분리 및 특성)

  • Yang, Soo-Jeong;Kim, Hyung-Moo;Ju, Ho-Jong
    • Korean Journal of Organic Agriculture
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    • v.23 no.1
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    • pp.133-148
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    • 2015
  • Although several adverse effects have been increased in recent years, synthetic agro-chemicals have been widely used to control diseases on paprika. This research was conducted to isolate and to characterize the antagonistic microorganism to control major paprika diseases, gray mold rot, fruit and stem rot, phytophthora blight, sclerotium rot, and wilt disease. Analysis of the fatty acid and analysis of the 16S rDNA gene sequence revealed that YKJ2 isolated in this research belongs to a group of Burkholderia cepacia. Specially, 16S rDNA gene sequence of YKJ2 showed 99% of sequence similarity with B. cepacia. Observation through the optical microscope revealed that YKJ2 was effective on suppression of the spore germination and the hyphal growth of pathogens. YKJ2 treatment on pathogens induced marked morphological changes like hyphal swelling and degradation of cell wall. In the case of phytophthora blight, the zoosporangium formation was restrained. On the basis of the results of this study, we propose that an antagonistic microorganism, B. cepacia, found in this study naming as "B. cepacia strain YKJ2" and has great potential as one of biological control agents against major diseases of paprika.