Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
권호 표지

Isolation of Bacterial Strain Antagonistic to Pyricularia oryzae and Its Mode of Antifungal Action

  • Bae, Dong-Won (Department of Agricultural Biology, Gyeongsang National University) ;
  • Lee, Joon-Taek (Department of Agricultural Biology, Genetic Engineering Institute, Gyeongsang National University) ;
  • Son, Dae-Young (Genetic Engineering Institute, Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University) ;
  • Lee, Eun Sook (Department of Preparatory Oriental Medicine, Kyungsan University) ;
  • Kim, Hee-Kyu (Department of Agricultural Biology, Genetic Engineering Institute, Gyeongsang National University)
  • Published : 2000.12.01
Download PDF
⟨ Previous Next ⟩

Abstract

An antagonistic bacterium PM-1 which strongly inhyibits the growth of Pyricularia oryzae was isolated and identified as paenibacillus macerans. The antifungal substances of the strain PM-1 showed the broad antifungal spectra against P.oryzae races. Relating to the localization test, it was found that the antifungal substances existed not only in the cytoplasm but also in the culture supernatant, and importantly the antifungal activity of the latter was stronger than that of the former. The extracellular antifungal substances were extremely heat-stable up to $121^{\circ}C$ for 15 min. The substances were optimally produced at $20^{\circ}C$ and pH 10.0 in a potato dextrose broth. The culture filtrate of the strain PM-1 caused a partial swelling of the mycelia of P.oryzae, and it prevents the normal growth of the fungus as well. This result suggested that the antifungal substances secreted by the strain PM-1 potentially inhibited the germination of P.oryzae.

Keywords

References

  1. Annu. Rev. Phytopathol. v.24 Biosynthesis and functions of fungal melanin Bell, A. A.;M. H. Wheeler
  2. Annu. Rev. Phytopathol. v.30 Breeding rice for resistance to pests Bonman, J. M.;G. S. Khush;R. J. Nelson
  3. Annu. Rev. Phytopathol. v.26 Role of antibiosis in the biocontrol of plant disease Fravel, D. R.
  4. Agric. Biol. Chem. v.47 Screening of antifungal antibiotics according to activites inducing morphological abnormalities Gunji, S.;K. Arima;T. Beppu
  5. J. Am. Chem. Soc. v.91 Studies on polyoxins, antifungal antibiotics. VIII. The structure of polyoxins Isono, K.;K. Asahi;S. Suzuki
  6. J. Antibiotics v.23 Studies on validamycins, new antibiotics. II. Production and biological properties of validamycins A and B Iwasa, T.;E. Higashide;H. Yamamoto;M. Shibata
  7. Kor. J. Plant Pathol. v.11 Antifungal effects on plant pathogenic fungi and characteristics of antifungal substances produced by Bacillus subtilis SJ-2 isolated from sclerotia of Rhizoctonia solani Kim, B. S.;K. Y. Cho
  8. Kor. J. Plant Pathol v.14 Purification of antifungal antibiotic NH-B1 from actinomycete NH 50 to plant pathogenic fungi Kim, H. K.;B. S. Kim;S. S. Moon;B. K. Hwang
  9. Kor. J. Appl. Microbiol. Biotechnol. v.25 Biological control of Pyricularia oryzae spot with the antibiotic substances produced by Bacillus sp. KL-3 Kim, K. Y.;S. D. Kim
  10. Kor. J. Appl. Microbiol. Biotechnol. v.25 Isolation and structure determination of antifngal antibiotics from Bacillus subtilis YB-70, a powerful biocontrol agent Kim, Y. S.;J. K. Son;D. C. Moon;S. D. Kim
  11. Kor. J. Appl. Mocrobiol. Biotechnol. v.25 Antifungal activity of Bacillus sp. SS279 biocontrol of apple white rot fungus, Botryosphaetia dothidea Kim, S. S.;G. J. Joo;J. Y. Uhm;Y. J. Kim;K. O. Rhee
  12. Bergey's Manual of Systematic Bacteriology v.2 Krieg, N. R.;J. G. Holt
  13. Pestic. Sci. v.39 Microbiol fungicides the natural choice Lange, L.;J. Breinholt;F. W. Rasmussen;R.I. Nielsen
  14. J. Microbio. Biotechnol. v.9 Immunosupperssive activity of cepacidine A, a novel antifungal antibiotic prosuced by pseudomonas cepacia Lee, C.-H.;J. W. Suh;Y. H. Cho
  15. J. Microbiol. Biotechnol. v.8 Antifungal activity medium-chain (C6-C13) alkenals against, and their inhibitory effect on the plasma membrane H+-ATPase of Sacharomyces cerevisiae Lee, J. R.;S. H. Lee;I.Kubo;S. D. Hong
  16. Magnaporthe grisea (Pyricularia oryzae), The blast fungus Advances in pathology genetic of plant pathogenic fungi Leung, H.;M. Taga;D. S. Ingram;D. H. Williums
  17. Phytopathology v.76 Production and partial characterization of antifungal substances antagonistic to Monilinia fructicola from Bacillus subtilis Mckeen, C. D.;C. C. Reilly;P. L. Pusey
  18. Annu. Rev. Phytophathol. v.18 pathogen variability and host resistance in rice blast disease Ou, S. H.
  19. Pestic. Sci. v.39 Diversity of microbial aspects of biocontrol products Porter, N.;F. M. Fox
  20. Pestic. Sci. v.37 Technical and commercial aspects of biocontrol products Powel, K. A.;A. R. Jutsum
  21. Plant Dis. v.68 Fungicde resistance; A continuing challenge Staub, T.;D. Sozzi
  22. Biological control of rice leaf blast Sy, A. A.;L. Albertini;M. Moletti;R. S. Zeigler;S. A. Leong;P. S. Teng
  23. J. Antibiotics v.11 Blasticidin S, a new antibiotic Takeuchi, S.;K. Hirayama;K. Ueda;H. Sasaki;H. Yonehara
  24. J. Antibiotics v.18 A new antibiotic, kasugamycin Umezawa, H.;Y. Okami;T. Hashimoto;Y. Suhara;M. Hamada;T. Takeuchi
  25. J. Microbiol. Biotechnol. v.4 Isolation, physico-chemical properties, and biological activity of new thiopeptide antibiotics, kimorexins Yeo, W. H.;S. K. Kim;S. S. Kim;S. H. Yu;E. K. Park