• Korean Chemical Engineering Research
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• v.58 no.2
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• pp.280-285
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• 2020

We have developed a constitutive display system of the Pseudomonas fluorescens SIK W1 TliA lipase on the cell surface of Escherichia coli using E. coli outer membrane protein C (OmpC) as an anchoring motif, which is an economical compared to induced system. For the constitutive expression of truncated OmpC-TliA fusion proteins, gntT104 promoter was employed. Cell growth was not affected by over expression of fusion protein during entire culture time, suggesting cell lysis was not a problem. The localization of truncated OmpC-TliA fusion protein on the cell surface was confirmed by immunofluorescence microscopy and measuring whole cell lipase activity. Constitutively displayed lipase was very stable, retaining activity enantioselectivity throughout the five repeated reactions. These results suggest that OmpC from E. coli be a useful anchoring motif for displaying enzymes on the cell surface without any inducers, and this stable surface display system can be employed for a broad range of biotechnological applications.

• Journal of the Optical Society of Korea
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• v.15 no.3
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• pp.305-309
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• 2011

We present an efficient design approach for a plasmonic slot waveguide using a genetic algorithm. The analyzed structure consists of a nanometric slot in a thin metallic film embedded within a dielectric. To achieve high confinement without long propagation length, the thickness and width of the slot are optimally designed in order to optimize the figures of merit including mode confinement and propagation length. The optimized design is based on the finite element method and enhances the guiding and focusing of light power propagation.

• Proceedings of the KIEE Conference
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• 1999.07b
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• pp.969-971
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• 1999

Wavelet analysis is applying to many fields such as the time-frequency localization of a time series and a time varying data. In this paper, a statistical testing based Wavelet power spectrum analysis for the stationary Nino3 Sea Surface Temperature(SST) data was executed. Specially, the 95% confidence level for SST was effective in searching the periods of El-Nino using various wavelet basis functions.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.21 no.5
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• pp.69-76
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• 2020

The sound signal radiated from an underwater source is received by the hydrophone of the system, including multi-path time-delay and multi-path signal by sea surface and bottom reflection. The system using a time-delay between received signals for the source localization shows performance degradation due to incoherence by the multi-path propagation environment and the disturbance of a marine environment. Various types of array and signal processing have been used for robust source range and bearing estimation in this environment. In this paper, we use a line array composed of doublet array and an estimated time-delay correction method for robust localization performance in a multi-path propagation environment. Three doublet arrays are located on the same line, and the time-delay between signals received on each doublet array is estimated in a two-step procedure. The estimated time-delay value is obtained by the cross-correlation function and corrected by the interaction formula between the center-frequency of received signal and the geometry of the array with respect to aperture. By this proposed procedure, the range and bearing of source from array were calculated. In order to confirm the validity of the proposed method and array, we simulated localization and estimation using the Monte-Carlo method.

• Transactions of the Korean Society of Machine Tool Engineers
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• v.12 no.5
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• pp.34-39
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• 2003

The paper will present chip formation mechanism and surface integrity generation mechanism based on the systematical experimental tests. Some basic factors such as the end milling cutter tooth number, cutting forces, cutting temperature, cutting vibration the chip status, the surface roughness, the hardness distribution and the metallographic texture of the machined surface layer are involved. The chip formation mechanism is typical thermal plastic shear localization at high cutting speed with less number of shear ribbons and bigger shear angle than that at low speed, which means lack of chip deformation. The high cutting speed with much more cutting teeth will be beneficial to the reduction of cutting forces, enlarge machining stability mot depression of temperature increment anti-fatigability as well as surface roughness. The burrs always exist both at low cutting speed and at high cutting speed. So the deburring process should be arranged for milling titanium alloy in my case.

• The Korean Journal of Zoology
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• v.35 no.2
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• pp.149-160
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• 1992

Using the protein A-gold complex, the mvoabrillogenesis and actin localization of cultured myoblast were invastisated. In the superstructural changes of mvogenic cell during differentiation, pectoral myoblasts contained large nucleus and numerous ribosomes but no myofibrils during the first 24 hr of cultures. Mvoblast initiated to differentiate at 3-day of culture contained the primitive myofibrillar structure. At 96 hr of culture, the mvofibrillar structure showed reletively discernable Z band but pools defined A, H and M bands. The feature of sarcomeric structure showed more defined form at cultur 5 day. In the aspect of actin localization, actin wvas diffusely detected throughout the cytoplasm of myogenic cell and nucleus during the proliferating stage. At 72 hr of culture, with the appearantc oi primitive mvofibrils, gold particles were observed in surrounding of myofibrils but still presented in overall of cytoplasm, especially in the surface and lumen of endoplasmic reticulum. With the gradual increase of culture time, local distribution of actin was readily detected within cytoplasm. In the 5-day specimen of cultures, gold particles precisely indicate the sites of actin localifation within the sarcomere. These results indicate the time of onset of myofibrill appearance and the biosynthetic and incorporation pathway of actin molecules into sarcomeric structure during myofibrillogenesis. Thus, in the present study, the first mvoabrillar structure was detected at culture 3 day, and the initiation of assembly into a typical sarcmeric structure was observed at culture 5 day. It seems, however, that the course of events on myofibrillogenesis of cultured myoblasts can be changed with great dependence of culture conditions including the number and groluth rate of mononucleated mvoblasts after seeding although the fundamental process shows identical appearances.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.16 no.11
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• pp.7773-7780
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• 2015

Analysis of existing kettle and its heating system has been the topic for localization technology development. Test pieces are made, polished and etched for existing kettle analysis. Surface of test pieces is observed using SEM, the kettle is verified to be made by deep drawing process from Ferrite-Perlite material. The kettle is also identified to be plated $16{\sim}49{\mu}m$ of thickness with Nickel(16%). Also heat transfer characteristics based on hot wire arrangement is investigated and optimal hot wire system is developed. Developed control system detects overheating and stops the whole system on the long operating time. Developed kettle takes the performance evaluation test for volume expansion and satisfied for standard 'KS G3602'.

• Korean Journal of Remote Sensing
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• v.37 no.5_1
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• pp.1163-1175
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• 2021

In autonomous driving, road markings are an essential element for object tracking, path planning and they are able to provide important information for localization. This paper presents an approach to update and measure road surface markers with HD maps as well as matching using inverse perspective mapping. The IPM removes perspective effects from the vehicle's front camera image and remaps them to the 2D domain to create a bird-view region to fit with HD map regions. In addition, letters and arrows such as stop lines, crosswalks, dotted lines, and straight lines are recognized and compared to objects on the HD map to determine whether they are updated. The localization of a newly installed object can be obtained by referring to the measurement value of the surrounding object on the HD map. Therefore, we are able to obtain high accuracy update results with very low computational costs and low-cost cameras and GNSS/INS sensors alone.

• Transactions of the Korean Society for Noise and Vibration Engineering
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• v.21 no.12
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• pp.1120-1131
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• 2011

Researches using time reversal acoustics(TRA) for impact localization have been paid attention to recently. Dispersion characteristics of Lamb waves, which restrict the utility of classical nondestructive evaluation based on time-of-flight information, can be compensated through the application of TRA to Lamb waves on a plate. This study investigates the spatial focusing performance of time reversal Lamb waves on a plate using finite element analysis. In particular, the virtual sensor effect caused by multiple wave reflections at the boundaries of a plate is shown to enable the spatial focusing of Lamb waves though a very small number of surface-bonded piezoelectric(PZT) sensors are available. The time window size of forward response signals, are normalized with respect to the number of virtual active sensors. Then their effects on the spatial focusing performance of Lamb waves are investigated.

• Journal of the Korean Society of Manufacturing Technology Engineers
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• v.22 no.3
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• pp.452-458
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• 2013

Over the past several years, many studies have been carried out in the field of 3D data inspection systems. Several attempts have been made to improve the quality of manufactured parts. The introduction of laser sensors for inspection has made it possible to acquire data at a remarkably high speed. In this paper, a robust inspection technique for detecting defects in 3D pressed parts using laser-scanned data is proposed. Point cloud data are segmented for the extraction of features. These segmented features are used for shape matching during the localization process. An iterative closest point (ICP) algorithm is used for the localization of the scanned model and CAD model. To achieve a higher accuracy rate, the ICP algorithm is modified and then used for matching. To enhance the speed of the matching process, aKd-tree algorithm is used. Then, the deviation of the scanned points from the CAD model is computed.