• Title/Summary/Keyword: sunflower

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Purification and Properties of Sunflower Seed $\alpha$-Galactosidase by Affinity Chromatography. (Galactomannan 이용에 관한 연구;Affinity Chromatography법에 의한 해바라기씨 유래 $\alpha$-Galactosidase의 정제 및 성질)

  • 박귀근;김욱동;박영서;강종백;소림수행
    • Microbiology and Biotechnology Letters
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    • v.26 no.4
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    • pp.316-322
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    • 1998
  • An ${\alpha}$-D-galactosidase (${\alpha}$-D-galactoside galactohydrolase, EC 3. 2. 1. 22) from sunflower seed was purified by affinity chromatography using N-$\varepsilon$-aminocaproyl-${\alpha}$-D-galactopyranosylamine coupled to sepharose and its properties were examined. The specific activity of the purified enzyme, tested with p-nitrophenyl-${\alpha}$-D-galactopyranoside as substrate, was 291.66 units/mg protein, representing an 115-folds purification of the original crude extract. The final preparation obtained from by Sephadex G-25 chromatography showed a single band on SDS-polyacrylamide gel electrophoresis. The molecular weight was determined to be 42,000 by SDS-polyacrylamide gel electrophoresis. The purified galactosidase showed maximum activity at pH 4.5 and 55$^{\circ}C$, and was stable in the pH and temperature ranges of 4.0 to 5.0 and 30 to 55$^{\circ}C$, respectively. The enzyme activity was inhibited by Ag$\^$2+/, Hg$\^$2+/ and Co$\^$2+/. The enzyme activity was not affected considerably by treatment with other metal compounds. The enzyme liberated galactose from melibiose, raffinose, copra galactomannan, guar gum and locust bean gum by TLC, and also the hydrolysis rate of substrate was compared by HPLC.

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Agronomic Characteristics of Sunflower (Helianthus annuus L.) Collections (해바라기 수집종의 작물학적 특성)

  • Kim, In-Jae;Nam, Sang-Young;Lee, Yun-Ho;Kim, Seong-Jin;Choi, Seong-Yel;Rho, Chang-Woo;Lee, Jung-Gwan;Song, In-Gyu;Kim, Hong-Sig
    • Korean Journal of Plant Resources
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    • v.23 no.1
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    • pp.1-6
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    • 2010
  • To evaluate agronomic characteristics for the use of biodiesel crop, 328 collections of sunflower (Helianthus annus L.) were obtained from Genebank in Rural Development Adminstration (RDA). The necessary days from seeding to emergence of collections were from 7 to 12 days, and the days to flowering were widely distributed from 55 to 86 days. Stem length ranged from 131 to 345 cm with a mean of 259 cm, and the mean maturing days were 35 days. The number of head flower was 1~23 ea per plant, and the mean size of head flower was 17.6 cm with a range from 14.7 to 21.3 cm (72.8%). The mean seed number per head flower was 1,430 ea, and the weight of seed per plant ranged from 23 to 379 g with a mean of 91.4 g. The mean seed length was 11.7 mm with a range from 9.0 to 21.5 mm, and the mean diameter was 6.4 mm. The mean weight of seed per litter, 1000 grain weight and seed weight per plant were 322.5 g, 63.3 g and 204 g, respectively. Variation of number of head per plant was largest and weight of grain per plant was large in next among growth and grain characteristics. At the results of correlation analysis among characteristics, the seed diameter was getting bigger and the days for flowering dates were prolonged in the higher stem length plant, but the days to maturing and growth duration was shortened.

Dewaxing of Sunflower Seed Oil (해바라기 유(油)의 탈납)

  • Rhee, Joon-Shick
    • Korean Journal of Food Science and Technology
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    • v.11 no.2
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    • pp.112-117
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    • 1979
  • By using the existing caustic refining system with a minimum modification and by using a combination of various emulsifiers(0.2 % sodium hexametaphosphate. 0.05 % sodium lauryl sulfate and 0.001 % dioctyl sodium sulfosuccinate), a new economical dewaxing process for sunflower seed oil was developed in order to reduce the cost of the dewaxing process. The results indicate that the waxes can be removed satisfactorily from the sunflower seed oil by emulsifying. batchwise or continuously, the oil with the aqueous surfactant solution, followed by centrifugation at ambient temperatures $(16{\sim}27^{\circ}C)$. Dewaxing loss for the batch process was satisfactory for both low wax-and high wax-crude oil, whereas dewaxing loss for the continuous process needs to be improved. The results indicate that initial level of wax content (low wax vs. high wax) did not affect the loss for batch process (0.82 % vs. 0.62 %), but affected the loss for continuous processes, regardless of the type of mixing mode (2.28 % and 5.68 % for low wax-and high wax-oil, respectively). It was also noted that the type of mixing mode (centactor vs. static mixer) for the continuous process affected the loss, regardless of the wax content (5.2 % and 2.8 % for contactor and static mixer, respectively).

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Enzymatic Synthesis of Low Trans Fats Using Rice Bran Oil, Palm Stearin and High Oleic Sunflower Seed Oil (미강유, 팜스테아린 및 고올레인산 해바라기씨유를 이용한 저트랜스 유지의 효소적 합성)

  • Kim, Ji-Young;Lee, Ki-Teak
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.38 no.4
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    • pp.470-478
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    • 2009
  • Low trans fats were synthesized by interesterification of rice bran oil (RBO), palm stearin (PS) and high oleic sunflower seed oil (HO) using TLIM from Thermomyces lanuginosa. After 24-h reaction, physicochemical characteristics such as fatty acid and triacylglycerol composition, solid fat content, melting point, tocopherol, oryzanol and phytosterol contents were evaluated. Trans fatty acid contents of the produced low-trans fats showed less than 0.5 wt%. Mostly, triacylglycerol species in the products were palmitoyl-linoleoyl-oleoyl-glycerol (PLO), palmitoyl-oleoyl-oleoyl-glycerol (POO) and palmitoyl-oleoyl-palmitoyl-glycerol (POP). Total tocopherol contents ranged from 6.94 to 11.83 mg/100 g while $0.18{\sim}0.49$ mg/100 g of $\gamma$-oryzanol and $182.47{\sim}269.08$ mg/100 g of phytosterols were observed depending on the substrates ratios. When the content of PS in the reaction substrate was increased, solid fat content and slip melting points were increased.

Comparison of Physicochemical Properties and Antioxidative Activities of Sunflower Sprout According to Germination Day (발아일수에 따른 해바라기 싹의 이화학적 특성 및 항산화성 비교)

  • Roh, Kyung-Rea;Ko, Seong-Hee;Kim, Chul-Jai
    • Journal of the Korean Society of Food Culture
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    • v.27 no.1
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    • pp.66-74
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    • 2012
  • It is well known that sunflower (SF) sprout has more beneficial effects than SF seed due to increased levels of phytochemical components such as vitamins, total phenolics, and isoflavones during germination. This study investigated the physicochemical properties and antioxidative activities of SF seed during both germination and cultivation. In a proximate analysis, the water content of SF groat was 9.17% and then increased to 15.32% on the 11th day after seeding. On a dry weight basis, crude fat content decreased while the contents of carbohydrates, crude protein, and crude ash increased. As cultivation proceeded, the contents of minerals were in decreasing order of K, Mg, Ca, Na, Zn, Fe, Mn, and Cu, in which SF sprout cultivated for 9 or 11 days contained the highest mineral contents. Though vitamin C was not detected on SF groat, the content of vitamin C continuously increased up to the 5th day of cultivation and then decreased gradually. Vitamin E content in SF groat was higher than that in SF sprout. It was also found that the vitamin E content in SF sprout was the highest on the 5th day of cultivation. Daidzin was not detected in SF groat, but its concentration reached a maximum on the 5th day of cultivation in SF sprouts. Furthermore, higher amounts of daidzein were observed on the 3rd, 5th, and 9th days of cultivation. The highest total isoflavone content was observed on either the 3rd or 5th day of cultivation. The highest content of total phenolics was observed on the 5th of cultivation. When DPPH radical and peroxyl radical scavenging activities of SF sprout were measured in order to measure antioxidant efficacy, it was found that 5 day-cultivated SF sprout had the highest scavenging activities. In conclusion, SF sprout cultivated for 9 or 11 days was found to be a good source of minerals. Furthermore, the fifth-day after seeding was the optimal time for the production of SF sprout with effective natural antioxidant activity and high amounts of functional components such as vitamins, total phenolics, and isoflavones.

Mobilization of Heavy Metals Induced by Button Mushroom Compost in Sunflower

  • Lee, Jong-Jin;Lee, Heon-Hak;Kim, Sung-Chul;Yoo, Jeoung-Ah;Lee, Chan-Jung;Yoon, Min-Ho
    • Korean Journal of Soil Science and Fertilizer
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    • v.48 no.5
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    • pp.469-476
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    • 2015
  • Button mushroom compost (BMC) was prepared by fermenting the mixture of waste button mushroom bed collected from Boryeong area in South Korea (4): sawdust (8) : pig and fowl manure (1) for 40 days at $30^{\circ}C$. The BMC compromised diverse microorganisms including aerobic bacteria $8.1{\times}10^6cfu\;g^{-1}$, Gram negative bacteria $1.7{\times}10^7cfu\;g^{-1}$, genus Bacillus $6.4{\times}10^6cfu\;g^{-1}$, genus Pseudomonas $1.5{\times}10^4cfu\;g^{-1}$, actinomycetes $1.0{\times}10^4cfu\;g^{-1}$, and fungi $3.5{\times}10^3cfu\;g^{-1}$. BMC was used as a microbial inoculant for estimating the mobilization of heavy metals in soil or plant. When metal solubilization potential of BMC was assessed in a batch experiment, the inoculation of BMC was shown to increase the concentrations of water soluble Co, Pb, Cd, and Zn by 29, 26, 27, and 43% respectively, than those of non-inoculated soils. BMC-assisted growth promotion and metal uptake in sunflower (Helianthus annuus) was also evaluated in a pot experiment. In comparison with non-inoculated seedlings, the inoculation led to increase the growth of H. annuus by 17, 15, 18, and 21% respectively in Co, Pb, Cd, and Zn contaminated soils. Moreover, enhanced accumulation of Co, Pb, Cd, and Zn in the shoot and root systems was observed in inoculated plants, where metal translocation from root to the above-ground tissues was also found to be enhanced by the BMC. The apparent results suggested that the BMC could effectively be employed in enhancing phytoextraction from the soils contaminated with heavy metals such as Co, Pb, Cd, and Zn.

Effect of partially replacing soybean meal with sunflower meal with supplementation of multienzymes on growth performance, carcass characteristics, meat quality, ileal digestibility, digestive enzyme activity and caecal microbiota in broilers

  • Yaqoob, Muhammad Umar;Yousaf, Muhammad;Imran, Safdar;Hassan, Safdar;Iqbal, Waqar;Zahid, Muhammad Umer;Ahmad, Naveed;Wang, Minqi
    • Animal Bioscience
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    • v.35 no.10
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    • pp.1575-1584
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    • 2022
  • Objective: An experiment was conducted to evaluate the effects of partially replacing soybean meal (SBM) with sunflower meal (SFM) with added exogenous multienzymes (MEs) on various biological parameters in broilers. Methods: One week-old, 400 broiler chicks were randomly divided into four treatments (control, 3SFM, 6SFM, and 9SFM) with 5 replicates/treatment (20 chicks/replicate). Control diet was without SFM and MEs, while diets of 3SFM, 6SFM, and 9SFM treatments were prepared by replacing SBM with SFM at levels of 3%, 6%, and 9%, respectively, and were supplemented with MEs (100 mg/kg). Feeding trial was divided into grower (8 to 21 day) and finisher phases (22 to 35 day). External marker method was used to measure the nutrient digestibility. At the end of trial, twenty birds (one birds per replicate) with similar body weight were slaughtered for samples collection. Results: No significant effect of dietary treatments was found on all parameters of growth performance and carcass characteristics, except relative weight of bursa. Weight (25.0 g) and length (15.80 cm) of duodenum were significantly (p<0.05) higher in 3SFM than control. Lowest (p<0.05) villus height/crypt depth ratio was found in 3SFM and 9SFM than control. Most of meat quality parameters remained unaffected, however, highest pH of breast meat (6.16) and thigh meat (6.44) were observed in 9SFM and 3SFM, respectively. Lowest (p<0.05) cook loss of thigh meat was found in 6SFM (31.76%). Ileal digestibility of crude protein was significantly (p<0.05) higher in 3SFM (72.35%) than control (69.46%). In addition, amylase (16.87 U/mg) and protease (85.18 U/mg) activities were significantly (p<0.05) higher in 3SFM than control. However, cecal microbial count remained unaffected. Conclusion: Partial replacement (up to 9%) of SBM with SFM, with added MEs can help to improve the nutrient digestibility, intestinal morphology, and digestive enzyme activities without affecting cecal microbial count and growth performance in broilers.

An Analysis on Characteristics of Surface Discharge on Polymer Insulator Using UV Detected Images (자외선 검출이미지를 이용한 폴리머 애자의 연면방전 특성해석)

  • Shong, Kil-Mok;Kim, Young-Seog;Park, Sang-Taek
    • Proceedings of the KIEE Conference
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    • 2009.04a
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    • pp.313-314
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    • 2009
  • In this paper, it's described the UV detected images on polymer insulators using UV camera. The patterns of UV detected images are classified into 3 types such as jellyfish(J), amoeba(A), sunflower(S). J type is detected by high electric field in air A type is detected by early surface discharge. And, S type is detected by the serious problem in surface of insulator.

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Fiber source and inclusion level affects characteristics of excreta from growing pigs

  • Mpendulo, Conference Thando;Chimonyo, Michael;Ndou, Saymore Petros;Bakare, Archibold Garikayi
    • Asian-Australasian Journal of Animal Sciences
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    • v.31 no.5
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    • pp.755-762
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    • 2018
  • Objective: The objective of the study was to determine the influence of varying fibrous diets on fecal characteristics of growing pigs. Methods: A total of 104 pigs (initial weight $18{\pm}2.0kg$) were used in the study. They were housed in individual pens and fed on diets containing maize cob, grass hay, lucerne hay, maize stover, and sunflower husk. These fibers were included at 0, 80, 160, 240, 320 and 400 g/kg. Fecal and urine samples were collected. Results: Fecal output was largest amongst pigs fed on diets containing grass hay and maize stover (p<0.05). Nitrogen content was highest in feces from pigs fed on sunflower husk (p<0.05). Pigs fed on diets containing maize stover and maize cobs produced the largest concentrations of short chain fatty acids. Acetate concentration was high in feces of pigs fed maize stover than those fed grass hay and lucerne hay (p<0.05). As the level of fiber inclusion increased, fecal consistency and nitrogen content increased linearly (p<0.05). Urea nitrogen decreased as the inclusion level increased across all the fibers (p<0.05), with maize cobs containing the largest content of urea nitrogen. As dietary fiber content increased, fecal nitrogen content also increased (p<0.05). Conclusion: It was concluded that different fiber sources influence fecal characteristics, thereby having different implications on pig waste management. It is vital to monitor fiber inclusion thresholds so as to easily manage environmental pollutants such as butyrate that contribute to odors.