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Enzymatic Synthesis of Low Trans Fats Using Rice Bran Oil, Palm Stearin and High Oleic Sunflower Seed Oil

미강유, 팜스테아린 및 고올레인산 해바라기씨유를 이용한 저트랜스 유지의 효소적 합성

  • Kim, Ji-Young (Dept. of Food Science and Technology, Chungnam National University) ;
  • Lee, Ki-Teak (Dept. of Food Science and Technology, Chungnam National University)
  • Published : 2009.04.30

Abstract

Low trans fats were synthesized by interesterification of rice bran oil (RBO), palm stearin (PS) and high oleic sunflower seed oil (HO) using TLIM from Thermomyces lanuginosa. After 24-h reaction, physicochemical characteristics such as fatty acid and triacylglycerol composition, solid fat content, melting point, tocopherol, oryzanol and phytosterol contents were evaluated. Trans fatty acid contents of the produced low-trans fats showed less than 0.5 wt%. Mostly, triacylglycerol species in the products were palmitoyl-linoleoyl-oleoyl-glycerol (PLO), palmitoyl-oleoyl-oleoyl-glycerol (POO) and palmitoyl-oleoyl-palmitoyl-glycerol (POP). Total tocopherol contents ranged from 6.94 to 11.83 mg/100 g while $0.18{\sim}0.49$ mg/100 g of $\gamma$-oryzanol and $182.47{\sim}269.08$ mg/100 g of phytosterols were observed depending on the substrates ratios. When the content of PS in the reaction substrate was increased, solid fat content and slip melting points were increased.

미강유, 팜스테아린 및 고올레인산 해바라기씨유의 효소적 interesterification을 통하여 저트랜스 유지를 합성하였고, 합성 시 Thermomyces lanuginose로부터 획득된 TLIM 효소를 이용하였다. 반응 기질의 비율에 따라 합성된 저트랜스 유지의 solid fat content(SFC)를 살펴본 결과, 전체적으로 고올레인산 해바라기씨유의 비율이 높아질수록 각 온도에 해당하는 SFC는 감소하는 경향을 보인 반면, 팜스테아린의 비율이 높아질수록 SFC는 증가하는 경향을 보였다. DSC를 통한 흡열 및 발열 곡선 분석결과, 팜스테아린의 함량이 높아질수록 피크가 높은 온도 쪽으로 이동하는 것을 확인할 수 있었다. 저트랜스 유지의 지방산 조성을 살펴본 결과, palmitic acid(C16:0), oleic acid(C18:1), linoleic acid(C18:2)가 주요 지방산들로써 전체 지방산 조성의 90% 이상을 차지하고 있었고, 총 트랜스지방산은 비율별로 0.5 area%의 수치를 보이며, 1% 미만의 낮은 함량을 보였다. Triacylglycerol (TAG) 조성을 reverse-phase HPLC를 이용하여 분석한 결과, 주요 TAG 조성은 OOL, PLO, PLP, OOO, POO, POP, POS 등으로 측정되었으며, 그 중 PLO와 POO 및 POP가 전체 조성의 50% 이상을 차지하였다. 한편, 여러 비율별로 합성된 저트랜스 유지의 총 tocopherol은 $6.94{\sim}11.83$ mg/100 g의 함량을 보였고, oryzanol은 $0.18{\sim}0.49$ mg/100 g의 함량을 보였으며, phytosterol 함량은 전체적으로 $182.48{\sim}269.07$ mg/100 g 사이의 다양한 수치를 나타내었다.

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