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Biochemical Assessment of Deer Velvet Antler Extract and its Cytotoxic Effect including Acute Oral Toxicity using an ICR Mice Model (ICR 마우스 모델을 이용한 녹용 추출물의 생화학적 평가 및 급성 경구 독성을 포함한 세포 독성 효과)

  • Ramakrishna Chilakala;Hyeon Jeong Moon;Hwan Lee;Dong-Sung Lee;Sun Hee Cheong
    • Journal of Food Hygiene and Safety
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    • v.38 no.6
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    • pp.430-441
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    • 2023
  • Velvet antler is widely used as a traditional medicine, and numerous studies have demonstrated its tremendous nutritional and medicinal values including immunity-enhancing effects. This study aimed to investigate different deer velvet extracts (Sample 1: raw extract, Sample 2: dried extract, and Sample 3: freeze-dried extract) for proximate composition, uronic acid, sulfated glycosaminoglycan, sialic acid, collagen levels, and chemical components using ultra-performance liquid chromatography-quadrupole-time-of-light mass spectrometry. In addition, we evaluated the cytotoxic effect of the deer velvet extracts on BV2 microglia, HT22 hippocampal cells, HaCaT keratinocytes, and RAW264.7 macrophages using the cell viability MTT assay. Furthermore, we evaluated acute toxicity of the deer velvet extracts at different doses (0, 500, 1000, and 2000 mg/kg) administered orally to both male and female ICR mice for 14 d (five mice per group). After treatment, we evaluated general toxicity, survival rate, body weight changes, mortality, clinical signs, and necropsy findings in the experimental mice based on OECD guidelines. The results suggested that in vitro treatment with the evaluated extracts had no cytotoxic effect in HaCaT keratinocytes cells, whereas Sample-2 had a cytotoxic effect at 500 and 1000 ㎍/mL on HT22 hippocampal cells and RAW264.7 macrophages. Sample 3 was also cytotoxic at concentrations of 500 and 1000 ㎍/mL to RAW264.7 and BV2 microglial cells. However, the mice treated in vivo with the velvet extracts at doses of 500-2000 mg/kg BW showed no clinical signs, mortality, or necropsy findings, indicating that the LD50 is higher than this dosage. These findings indicate that there were no toxicological abnormalities connected with the deer velvet extract treatment in mice. However, further human and animal studies are needed before sufficient safety information is available to justify its use in humans.

Geochemical Modeling on Behaviors of Radionuclides (U, Pu, Pd) in Deep Groundwater Environments of South Korea (한국 심부 지하수 환경에서의 방사성 핵종(우라늄, 플루토늄, 팔라듐)의 지화학적 거동 모델링)

  • Jaehoon Choi;SunJu Park;Hyunsoo Seo;Hyun Tai Ahn;Jeong-Hwan Lee;Junghoon Park;Seong-Taek Yun
    • Economic and Environmental Geology
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    • v.56 no.6
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    • pp.847-870
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    • 2023
  • The safe disposal of high-level radioactive waste requires accurate predictions of the long-term geochemical behavior of radionuclides. To achieve this, the present study was conducted to model geochemical behaviors of uranium (U), plutonium (Pu), and palladium (Pd) under different hydrogeochemical conditions that represent deep groundwater in Korea. Geochemical modeling was performed for five types of South Korean deep groundwater environment: high-TDS saline groundwater (G1), low-pH CO2-rich groundwater (G2), high-pH alkaline groundwater (G3), sulfate-rich groundwater (G4), and dilute (fresh) groundwater (G5). Under the pH and Eh (redox potential) ranges of 3 to 12 and ±0.2 V, respectively, the solubility and speciation of U, Pu, and Pd in deep groundwater were predicted. The result reveals that U(IV) exhibits high solubility within the neutral to alkaline pH range, even in reducing environment with Eh down to -0.2 V. Such high solubility of U is primarily attributed to the formation of Ca-U-CO3 complexes, which is important in both G2 located along fault zones and G3 occurring in granitic bedrocks. On the other hand, the solubility of Pu is found to be highly dependent on pH, with the lowest solubility in neutral to alkaline conditions. The predominant species are Pu(IV) and Pu(III) and their removal is predicted to occur by sorption. Considering the migration by colloids, however, the role of colloid formation and migration are expected to promote the Pu mobility, especially in deep groundwater of G3 and G5 which have low ionic strengths. Palladium (Pd) exhibits the low solubility due to the precipitation as sulfides in reducing conditions. In oxidizing condition, anionic complexes such as Pd(OH)3-, PdCl3(OH)2-, PdCl42-, and Pd(CO3)22- would be removed by sorption onto metal (hydro)oxides. This study will improve the understanding of the fate and transport of radionuclides in deep groundwater conditions of South Korea and therefore contributes to develop strategies for safe high-level radioactive waste disposal.

A Safety Survey for Residual Pesticides in Agricultural Products in Meal-kits (밀키트(가정간편식) 중 농산물의 잔류농약 안전성 조사)

  • Sung-min Song;Yoo Jung Sun;Hyun-Jung Seo;Hyun Ho Han;Ga Hye Lee;Jung-Im Kim;Meyong-Hee Kim;Myung-Je Heo;Mun-Ju Kwon
    • Journal of Food Hygiene and Safety
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    • v.38 no.6
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    • pp.457-463
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    • 2023
  • To investigate residual pesticide levels in agricultural products contained in Meal-kits, 27 Meal-kit products were collected from marts, Meal-kit shops, and online stores in Incheon City, South Korea. Seventy-six vegetable and thirty-seven mushroom products were analyzed for residual levels of 339 pesticides. Residual pesticides were detected in 23 out of 76 vegetables and were not present in the 37 mushroom products. The residual pesticide detection rate was 20.4% (23/113 cases). The pesticides famoxadone 0.034 mg/kg (standard: 0.01 mg/kg or less, PLS) and fenpyroximate 0.302 mg/kg (standard: 0.01 mg/kg or less, PLS) exceeded their maximum residue levels (MRL). This survey revealed that various types of pesticides remain in agricultural products in Meal-kits. Due to the nature of Meal-kit products, there is no separate standard for residual pesticides in agricultural products. Therefore, continuous monitoring of residual pesticides is necessary.

A study on the status of dietary behavior and nutrient intakes according to the gender and the level of physical activity of adolescents: using the 7th (2017-2018) Korea National Health and Nutrition Examination Survey (청소년의 성별과 신체활동 수준에 따른 식행동 및 영양섭취실태: 제7기 2,3차년도 (2017-2018년) 국민건강영양조사 자료 이용)

  • Seo Yeon Kim;Sun A Choi;Jeong Ok Rho
    • Journal of Nutrition and Health
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    • v.56 no.6
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    • pp.683-701
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    • 2023
  • Purpose: The purpose of the study was to analyze the status of dietary behavior and nutrition intakes according to the gender and the level of physical activity of adolescents. Methods: Data from 659 adolescents aged 13 to 18 years who participated in the Korea National Health and Nutrition Examination Survey (2017-2018) were analyzed. Collected data were analyzed using complex sampling data analysis with SPSS v. 27.0. Results: The adolescents were categorized into three groups based on their physical activity as follows: The first group with high physical activity (8.9%), a second group with normal physical activity (29.7%), and a third group without physical activity (61.4%). Boys showed significantly higher physical activity than the girls. Adolescents with high physical activity had a significantly lower body mass index than adolescents without physical activity, but their meal regularity, subjective health status, and recognition of body image were significantly higher. In terms of biochemical factors, adolescents with high physical activity had lower triglycerides and cholesterol than adolescents without physical activity, while hemoglobin and hematocrit were significantly higher. In addition, the intakes of energy, carbohydrates, proteins, fat, vitamin B1, niacin, calcium, phosphorus, sodium, potassium, iron increased significantly as adolescents' physical activity increased. Conclusion: The physical activity of adolescents had a positive effect on dietary behavior, lifestyle, and nutritional factors. Therefore, it is necessary to increase participation in physical classes in schools and develop various programs considering the level of physical activity among adolescents.

Aggregate of Korea in 2022 (2022년 한국의 골재)

  • Sei Sun Hong;Jin Young Lee
    • Economic and Environmental Geology
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    • v.56 no.6
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    • pp.871-885
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    • 2023
  • In 2022, the total of 129 million m3 of aggregate was produced in Korea, a slightly decrease from the total production of 2021. Of these, about 44 million m3 of sand and about 84 million m3 of gravel were produced. About 41% of total quantity of aggregates were produced by permission and the rest were produced after declaration. It estimated that of the 129 million m3 of aggregates in Korea in 2022, about 54.9% was produced by screening crushed aggregate, by 32.8% by forest aggregate, 2.2% by land aggregate, 6.2% by marine aggregate and 3.1% by washing aggregate, and 0.3% by river aggregate. This indicates that screening crushed and forest aggregate are the main producers of domestic aggregate in 2022. Leading producing metropolitan governments were Gyeonggi-do, Gyeongsangnam-do, Chungcheongnam-do, Incheon, Jeollanam-do, Chungcheongbuk-do, Gangwon-do, Gyeongsangbuk-do in order decreasing volume. In 2022, aggregates were produced in 147 local governments, and the 10 leading producing local governments were, in descending order of volume, Hwaseong, Pocheon, Paju, Ongjin, Youngin, Gwangju, west EEZ, Incheon Seo-gu, Namyangju, Asan. The combined production of the 10 leading local governments accounted for 31% of the national total. And 44 local governments have produced aggregates of more than 1 million m3 each other. In 148 local governments that produced aggregate, a total of 800 active operations produced aggregate with 350 operations by river, land and forest aggregate, 450 operations by selective crushed and washing aggregate.

Screening of Natural Products for Anti-diabetic Activity and Analysis of Their Active Compounds (항당뇨 효능이 있는 천연물의 탐색 및 활성물질의 분석)

  • Hwa Sin Lee;Bo Bae Park;Sun Nyoung Yu;Min Ji Kim;Yun Jin Bae;Yi Rooney Lee;Ye Eun Lee;Si Yoon Kim;Yun Ho Shim;Soon Cheol Ahn
    • Journal of Life Science
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    • v.33 no.10
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    • pp.783-790
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    • 2023
  • Modern people have an increased incidence of metabolic diseases due to changed eating habits, and diabetes is considered the most significant metabolic disease. Given that existing diabetes treatments are accompanied by side effects, the aim of this study was to identify traditional natural products that have anti-diabetic activity. The potential anti-diabetic and antioxidant activities of natural products were examined using 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging assay, α-glucosidase assay, and protein tyrosine phosphatase 1B (PTP1B) inhibition assay. Methanol extracts of Ulmus davidiana var. japonica, Acer tegmentosum branches, Nelumbo nucifera seeds, and Carthamus tinctorius seeds were found to have high anti-diabetic activity and further fractionated with solvents using ethyl acetate and butanol. Consequently, the ethyl acetate fraction of C. tinctorius seeds (MG-11-E) with high α-glucosidase and PTP1B inhibitory activity was selected. MG-11-E was subjected to preparative thin layer chromatography, and fraction #6 showed high α-glucosidase and PTP1B inhibitory activity. Fraction #6 was analyzed and fractionated via high performance liquid chromatography with 50% methanol as the mobile phase, and anti-diabetic activity was observed in the sample that eluted after 4 min as a single peak. The α-glucosidase inhibitory activity exhibited by this sample seemed to be greater than the PTP1B inhibitory activity; thus, it was concluded that a greater anti-diabetic therapeutic effect may be achieved by combining this agent with natural products that inhibit PTP1B activity.

Terrain Shadow Detection in Satellite Images of the Korean Peninsula Using a Hill-Shade Algorithm (음영기복 알고리즘을 활용한 한반도 촬영 위성영상에서의 지형그림자 탐지)

  • Hyeong-Gyu Kim;Joongbin Lim;Kyoung-Min Kim;Myoungsoo Won;Taejung Kim
    • Korean Journal of Remote Sensing
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    • v.39 no.5_1
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    • pp.637-654
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    • 2023
  • In recent years, the number of users has been increasing with the rapid development of earth observation satellites. In response, the Committee on Earth Observation Satellites (CEOS) has been striving to provide user-friendly satellite images by introducing the concept of Analysis Ready Data (ARD) and defining its requirements as CEOS ARD for Land (CARD4L). In ARD, a mask called an Unusable Data Mask (UDM), identifying unnecessary pixels for land analysis, should be provided with a satellite image. UDMs include clouds, cloud shadows, terrain shadows, etc. Terrain shadows are generated in mountainous terrain with large terrain relief, and these areas cause errors in analysis due to their low radiation intensity. previous research on terrain shadow detection focused on detecting terrain shadow pixels to correct terrain shadows. However, this should be replaced by the terrain correction method. Therefore, there is a need to expand the purpose of terrain shadow detection. In this study, to utilize CAS500-4 for forest and agriculture analysis, we extended the scope of the terrain shadow detection to shaded areas. This paper aims to analyze the potential for terrain shadow detection to make a terrain shadow mask for South and North Korea. To detect terrain shadows, we used a Hill-shade algorithm that utilizes the position of the sun and a surface's derivatives, such as slope and aspect. Using RapidEye images with a spatial resolution of 5 meters and Sentinel-2 images with a spatial resolution of 10 meters over the Korean Peninsula, the optimal threshold for shadow determination was confirmed by comparing them with the ground truth. The optimal threshold was used to perform terrain shadow detection, and the results were analyzed. As a qualitative result, it was confirmed that the shape was similar to the ground truth as a whole. In addition, it was confirmed that most of the F1 scores were between 0.8 and 0.94 for all images tested. Based on the results of this study, it was confirmed that automatic terrain shadow detection was well performed throughout the Korean Peninsula.

Development of Weight Estimation Equations and Weight Tables for Larix kaempferi and Pinus rigida Stand (일본잎갈나무와 리기다소나무의 중량추정식 및 중량표 개발)

  • Jintaek Kang;Chiung Ko;Jeongmuk Park;Jongsu Yim;Sun-Jeong Lee;Myoungsoo Won
    • Journal of Korean Society of Forest Science
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    • v.112 no.4
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    • pp.472-489
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    • 2023
  • This study was conducted to derive the optimal estimation equations for deriving the green and dry weights of Larix kaempferi (Japanese larch) and Pinus rigida (Rigida pine), which are major coniferous tree species in South Korea. The equations were then used to develop weight tables. Table development began with the sampling of 150 L. kaempferi and 90 P. rigida trees distributed throughout the national scale, after which green weights were measured on-site. Samples from each stand were then collected, and their dry weights were measured in a laboratory. The equation used to calculate green and dry weights was divided into a one-variable formula that uses only the diameter at breast height (DBH) and a two-variable equation that employs DBH and height. The equations used to estimate the green and dry weights of logs were divided into one- and two-variable equations using DBH. Statistical data, such as the fitness index (FI), root mean square error, standard error of estimation, and residual diagram, were used to verify the suitability of the estimation equations. Applicability was examined by calculating weights using the derived optimal equations. The equation W = bD+cD2 was used in measurements involving only DBH, whereas the equation W = aDbHc was employed in cases involving both diameter and height at breast height. The FI of W = bD+cD2 was 0.91, while that of W = aDbHc was 0.95, both of which are high values. With these estimation formulas, weight tables for the green and dry weights of L. kaempferi and P. rigida were prepared and compared with weight tables created 20 years ago. The green and dry weight tables of both species were larger.

Comparison of NDVI in Rice Paddy according to the Resolution of Optical Satellite Images (광학위성영상의 해상도에 따른 논지역의 정규식생지수 비교)

  • Jeong Eun;Sun-Hwa Kim;Jee-Eun Min
    • Korean Journal of Remote Sensing
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    • v.39 no.6_1
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    • pp.1321-1330
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    • 2023
  • Normalized Difference Vegetation Index (NDVI) is the most widely used remote sensing data in the agricultural field and is currently provided by most optical satellites. In particular, as high-resolution optical satellite images become available, the selection of optimal optical satellite images according to agricultural applications has become a very important issue. In this study, we aim to define the most optimal optical satellite image when monitoring NDVI in rice fields in Korea and derive the resolution-related requirements necessary for this. For this purpose, we compared and analyzed the spatial distribution and time series patterns of the Dangjin rice paddy in Korea from 2019 to 2022 using NDVI images from MOD13, Landsat-8, Sentinel-2A/B, and PlanetScope satellites, which are widely used around the world. Each data is provided with a spatial resolution of 3 m to 250 m and various periods, and the area of the spectral band used to calculate NDVI also has slight differences. As a result of the analysis, Landsat-8 showed the lowest NDVI value and had very low spatial variation. In comparison, the MOD13 NDVI image showed similar spatial distribution and time series patterns as the PlanetScope data but was affected by the area surrounding the rice field due to low spatial resolution. Sentinel-2A/B showed relatively low NDVI values due to the wide near-infrared band area, and this feature was especially noticeable in the early stages of growth. PlanetScope's NDVI provides detailed spatial variation and stable time series patterns, but considering its high purchase price, it is considered to be more useful in small field areas than in spatially uniform rice paddy. Accordingly, for rice field areas, 250 m MOD13 NDVI or 10 m Sentinel-2A/B are considered to be the most efficient, but high-resolution satellite images can be used to estimate detailed physical quantities of individual crops.

A Study on Changes in Consumption Behavior due to the Risk of the COVID-19 Pandemic (COVID-19 팬데믹 위험으로 인한 소비행동의 변화 연구)

  • Oh, Jong-chul;Lee, Yu-sun;Kim, Jae-hong
    • Journal of Venture Innovation
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    • v.5 no.2
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    • pp.49-66
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    • 2022
  • This study intends to examine how the perception of covid-19 risk affects consumers' consumption behavior based on previous studies in a situation where the spread of covid-19 is prolonged. This study demonstrates how consumers' perception of covid-19 risk affects online and offline consumption behavior through the perceived severity, perceived vulnerability, coping effectiveness, and self-efficacy of the revised protective motivation theory (Rogers, 1983). We want to test it through analysis. In order to achieve the purpose of this study, consumers living in Seoul and Gyeonggi Province who have purchased within the past 3 months were selected as a sample. In addition, variable data such as risk perception of covid-19, perceived severity, perceived vulnerability, coping effectiveness, self-efficacy, online purchase attitude and purchase intention, offline purchase attitude and purchase intention were collected through the questionnaire.A total of 363 copies of valid responses were tested to test the hypothesis of the relationship between variables through the covariance structure model. The analysis results of this study were first, that covid-19 risk perception had a significant positive (+) effect on perceived severity, perceived vulnerability, and coping effectiveness. Second, perceived severity and perceived vulnerability were found to have a significant positive (+) effect on offline purchasing attitude. Third, perceived severity, perceived vulnerability, coping plan effectiveness, and self-efficacy were all found to have significant positive (+) effects on online purchase attitude. Finally, it was found that offline purchase attitude and online purchase attitude had a significant positive (+) effect on offline purchase intention and online purchase intention, respectively. Also, it was found that online purchase attitude had a negative (-) effect on offline purchase intention. The results of this analysis will provide meaningful implications for the establishment of strategies for distribution channels according to the social risk of infectious diseases.