• Animal Bioscience
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• v.35 no.8
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• pp.1162-1173
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• 2022

Objective: This study aimed to investigate the fermentation profiles, bacterial community and predicted metabolic characteristics of Sudangrass (Sorghum sudanense Stapf.) during ensiling. Methods: First-cutting Sudangrass was harvested at the vegetative stage and ensiled in laboratory-scale silos (1 L capacity). Triplicate silos were sampled after 1, 3, 7, 15, 30, and 60 days of ensiling, respectively. The bacterial communities on day 3 and 60 were assessed through high-throughput sequencing technology, and 16S rRNA-gene predicted functional profiles were analyzed according to the Kyoto encyclopedia of genes and genomes using Tax4Fun. Results: The Sudangrass silages showed good fermentation quality, indicated by higher lactic acid contents, and lower pH, butyric acid and ammonia nitrogen contents. The dominant genus Lactococcus on day 3 was replaced by Lactobacillus on day 60. The metabolism of amino acid, energy, cofactors and vitamins was restricted, and metabolism of nucleotide and carbohydrate was promoted after ensiling. The 1-phosphofructokinase and pyruvate kinase of bacterial community seemed to play important roles in stimulating the lactic acid fermentation, and the promotion of arginine deiminase could help lactic acid bacteria to tolerate the acidic environment. Conclusion: High-throughput sequencing technology combined with 16S rRNA gene-predicted functional analyses revealed the differences during the early and late stages of Sudangrass ensiling not only for distinct bacterial community but also for specific functional metabolites. The results could provide a comprehensive insight into bacterial community and metabolic characteristics to further improve the silage quality.

• Journal of The Korean Society of Grassland and Forage Science
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• v.11 no.4
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• pp.258-263
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• 1991

There are ten NLCF's recommended sorghum-sudangrass hybrids(Sorghum bicolor (L.) Moench), but they have not been evaluated under same environmental conditions. Therefore, a field experiment was conducted to campare forage yield and quality of the NLCF's recommended cultivars of sorghum-sudangrass hybrids at the experimental forage field, College of Agriculture, SNU, Suweon over two years. Among the ten cultivars used, G83F and NC+855 were classified as early maturing cultivars. Pioneer 988 and Sordan 79 were more resistant to leaf blight than the other hybrids. Jumbo, Sordan 79 and SX-17 were susceptible to lodging. No significant differences in dry matter yield were found among the cultivars used, but the dry matter yields of Pioneer 988, TE-Haygrazer and G83F were slightly higher than the other hybrids in 1989 and those of NC+855 and Pioneer 988 were higher in 1990. There were no marked cultivar differences in CP, ADF, NDF and RFV at the first, second and third cuts in 1990, but the ADF, NDF and RFV of sorghum-sudangrass hybrids at the third cut showed more higher than those of the first and second cut forage. In conclusion, there are no forage yield differences among the ten NLCF's recommended sorghum-sudangrass hybrids.

• Journal of The Korean Society of Grassland and Forage Science
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• v.30 no.2
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• pp.109-114
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• 2010

This experiment was carried out to know adaptability and forage production and quality of sorghum and sorghum ${\times}$ sudangrass and sudangrass hybrids at paddy field from 2007 to 2008 at Chonnam province. Among growth characters of six Hybrids, 'SX17' hybrid was somewhat strong for waterlogging, and higher sugar content and very good at the 2nd regrowth and disease resistance. Fresh yield of 'SX17' hybrid was the highest as 89,192kg per ha among 6 Sorghum and Sorghum ${\times}$ Sudangrass and Sudangrass hybrids. The dry matter yield of 'SX17' hybrid was also the highest as 21,038 kg per ha. The result of this study showed that 'SX17' hybrid had good growth characters and forage productivity and crude protein (CP) and in vitro dry matter digestibility (IVDMD).

• Journal of The Korean Society of Grassland and Forage Science
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• v.31 no.2
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• pp.119-126
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• 2011

This experiment was carried out to know adaptability and forage production and quality of sorghum, sorghum ${\times}$ sudangrass and sudangrass hybrids depend on drained condition at paddy field from 2007 to 2008 at Chungnam province. Growth, forage production and quality of sorghum, sorghum ${\times}$ sudangrass and sudangrass hybrids showed more well drained condition than poorly drained condition at paddy field. Among growth characteristics, 'SS405' hybrids were somewhat strong for waterlogging, then and good at stem diameter, disease resistance. The dry yield of 'SS405' hybrid at poorly drained paddy field was the highest as 12,938 kg per ha. Fresh yield of poorly drained paddy field was 52.7% compared to that of well drained paddy field. The dry matter yield of poorly drained paddy field was the lower as 66.4% than that of well drained condition. ADF (acid detergent fiber), NDF (neutral detergent fiber), CP (crude protein) and IVDMD (in vitro dry matter digestibility) in poorly drained paddy field were 90.3%, 100.6%, 85.7% and 89.6% level compared to well drained paddy field.

• Korean Journal of Soil Science and Fertilizer
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• v.28 no.4
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• pp.319-326
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• 1995

This study was conducted to find out the effect of natural zeolite addition on the purification of livestock farming wastewater and the application of zeolite used for purification of livestock farming wastewater on growth of sudangrass. In a semi-continuous process it was found that COD, $NH_4-N$, $NO_3-N$ and T-N contents in the effluent solution decreased with the treatment of natural zeolite. The zeolite addition was effective on the purification of livestock farming wastewater. By the results of application for sudangrass cultivation, the accumulative N-uptake contents by sudangrass were lower than that of control(35kg/10a) at early growth stage, but were higher at the late growth stage.

• Journal of Environmental Science International
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• v.24 no.11
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• pp.1501-1511
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• 2015

As essential trace elements, copper and zinc play important roles in many physiological events in plants. In excess, however, these elements can limit plant growth. This study selected a heavy metal-tolerant plant by analyzing seed germination and biomass of alfalfa (Medicago sativa), canola (Brassica campestris subsp. napus var. nippo-oleifera), Chinese corn (Setaria italica), and a sorghum-sudangrass hybrid (Sorghum bicolor ${\times}$ S. sudanense), and determined heavy metal uptake capacity by analyzing biomass, chlorophyll a fluorescence, and heavy metal contents under high external copper or zinc levels. The seed germination rate and biomass of the sorghum-sudangrass hybrid were higher under copper or zinc stress compared to the other three plants. The plant biomass and photosynthetic pigment contents of the sorghum-sudangrass hybrid seedlings were less vulnerable under low levels of heavy metals (${\leq}50ppm$ copper or ${\leq}400ppm$ zinc). The maximum quantum yield of PSII ($F_v/F_m$) and the maximum primary yield of PSII ($F_v/F_o$) decreased with increasing copper or zinc levels. Under high copper levels, the decline in $F_v/F_m$ was caused only by the decline in $F_m$, and was accompanied by an increase in non-photochemical quenching (NPQ). The $F_v/F_m$ declined under high levels of zinc due to both a decrease in the maximum fluorescence ($F_m$) and an increase in the initial fluorescence ($F_o$), and this was accompanied by a marked decrease in photochemical quenching (qP), but not by an increase in NPQ. Accumulations of copper and zinc were found in both aboveand below-ground parts of plants, but were greater in the below-ground parts. The uptake capacity of the sorghum-sudangrass hybrid for copper and zinc reached 4459.1 mg/kg under 400 ppm copper and 9028.5 mg/kg under 1600 ppm zinc. Our results indicate that the sorghum-sudangrass hybrid contributes to the in situ phytoremediation of copper or zinc polluted soils due to its high biomass yield.

• Korean Journal of Organic Agriculture
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• v.20 no.1
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• pp.59-70
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• 2012

This experiments was conducted to investigate the influence on the growth of Sorghum ${\times}$ Sudangrass hybrid by use of various organic fertilizer and investigated their productivity, feed value and stock carrying capacity. In the results, the application of fermented poultry manure showed 93.6ton/ha, 19.6ton/ha, 1.12ton/ha, 11.31ton/ha of fresh yield, dry matter yield, crude protein (CP) yield and total digestible nutrients (TDN) yield but there were no significant differences with the application of poultry by-product compost (poultry manure with sawdust) and swine by-product compost (swine manure with sawdust). And the average value of $K_{CP}$ and $K_{TDN}$ showed the highest one as 9.45head/ha/yr in the application of fermented poultry manure. However, organic matter content of fermented poultry manure to the lower portion of 32.1% compared to other organic fertilizers could imagine that mineralization of fermented poultry manure was fairly advanced and plant used most of nitrogen in fermented poultry manure, so productivity of Sorghum ${\times}$ Sudangrass hybrid was shown more than other organic fertilizers. If other organic fertilizers use continuous, this difference can be considered to be reduced further. As a result, fermented poultry manure is better than other organic fertilizers in productivity, feed value and stock carrying capacity due to the higher content of mineralizable matters. In addition, mixing poultry manure with cow and swine manure is better choice because cow and swine manure will meet a low organic matter in poultry manure.

• Journal of The Korean Society of Grassland and Forage Science
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• v.34 no.2
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• pp.81-86
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• 2014

This study was conducted to evaluate the effect of different seeding dates on agronomic characteristics, forage quality, dry matter (DM) and dry matter digestible (DDM) yields of sudangrass hybrid (Sorghum bicolor(L.) Moench) and oat (Avena sativaL.) at Seongju in Kyeongbuk from 2012 to 2013. The experiment was arranged in randomized complete block design with three replications. Sudangrass was seeded five times seeding dates of 10 days interval ranging from $1^{th}$ August to $10^{th}$ September in 2012 and 2013. Oat was seeded three times seeding dates of 10 days interval ranging from $20^{th}$ August to $10^{th}$ September in 2013. Sudangrass was harvested on $26^{th}$ October, 2012 and $2^{nd}$ November, 2013. Plant length and DM content decreased with delaying seeing date. The DM and DDM yields of sudangrass were higher (P<0.05) in first seeding date than all other seeding dates and decreased with delaying seeding dates (P<0.05). The DM and DDM yields of oat were higher (P<0.05) in first and second seeding dates than third seeding date. The results of this study indicated that early seeding of sudangrass would be a recommended than late seeding after $20^{th}$ of August, and late seeding of oat after $30^{th}$ August would be recommened for DM and DDM yields in the Southern Korea.

• Korean Journal of Soil Science and Fertilizer
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• v.45 no.1
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• pp.51-58
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• 2012

Salinity is one of the most relevant abiotic factor limiting crop yield and its net primary productivity. In addition, salinity induces an increased stress ethylene synthesis in plants which, in turn, exacerbate the responses to the stressor. Bacterial single or co-inoculation effect was tested using previously characterized plant growth promoting (PGP) bacteria Brevibacterium iodinum RS16 and Methylobacterium oryzae CBMB20 on maize and sorghum-sudan grass hybrid under different concentrations of NaCl. Non-inoculated maize and sorghum-sudangrass hybrid showed 33.4% and 20.0% reduction in seed germination under highest NaCl (150 mM) level tested. However, under the same NaCl concentration, co-inoculation with B. iodinum RS16 and M. oryzae CBMB20 PGP strains increased the seed germination in maize (16.7%) and sorghum-sudangrass hybrid (4.4%). In Gnotobiotic growth pouch experiments conducted for maize and sorghum-sudangrass hybrid, co-inoculation of PGP B. iodinum RS16 and M. oryzae CBMB20 mitigated the salinity stress and promoted root length by 22.9% and 29.7%, respectively. Thus the results of this study could help in development of potential bioinoculants that may be suitable for crop production under saline conditions.

• Korean Journal of Soil Science and Fertilizer
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• v.47 no.1
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• pp.1-7
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• 2014

The objective of this study was to determine the effect of single and co-inoculation of plant growth promoting bacteria (PGPB) on early plant growth in Saemangeum reclaimed soil. Plant growth promoting Brevibacterium iodinum RS16 and Methylobacterium oryzae CBMB20 were inoculated on maize (Zea mays L.) and sorghum-sudangrass hybrid (Sorghum bicolor L.) grown in Saemangeum reclaimed soil. Single and co-inoculation of B. iodinum RS16 and M. oryzae CBMB20 increased plant height, dry biomass accumulation and macro-nutrient accumulation of maize and sorghum-sudangrass hybrid. M. oryzae CBMB20 treatment increased plant height in maize by 41.2% at 30 days after sowing (DAS), shoot dry weight and total dry weight compared to non-inoculated treatment. Macro-nutrient accumulation (N and P) in maize roots was significantly increased with co-inoculation treatment, K and Ca content was significantly increased at B. iodinum RS16 treatment compared to non-inoculated treatment. Macro-nutrient accumulation (P, K, Ca and Mg) in shoot was higher with M. oryzae CBMB20 treatment compared to non-inoculated treatment. In case of sorghum-sudangrass hybrid, co-inoculation treatment showed 33.7% increase in plant height compared to non-inoculated treatment at 30 DAS. M. oryzae CBMB20 treatment increased root dry weight and total dry weight, macro-nutrient accumulation in roots and N, Ca and Mg accumulation in shoot compared to non-inoculated treatment. P and K accumulation in shoot was significantly increased at co-inoculation treatment compared to non-inoculated treatment. This pot culture experiment demonstrated that single and co-inoculation of B. iodinum RS16 and M. oryzae CBMB20 increased the early growth and nutrient accumulation of maize and sorghum-sudangrass hybrid.