DOI QR코드

DOI QR Code

Effect of Brevibacterium iodinum RS16 and Methylobacterium oryzae CBMB20 Inoculation on Seed Germination and Early Growth of Maize and Sorghum-sudangrass hybrid Seedling under Different Salinity Levels

  • Kim, Ki-Yoon (Department of Environmental and Biological Chemistry, Chungbuk National University) ;
  • Hwang, Seong-Woong (Division of Reclaimed Land Agriculture Research, National Institute of Crop Science) ;
  • Saravanan, Venkatakrishnan Sivaraj (Department of Microbiology, Indira Gandhi College of Arts and Science) ;
  • Sa, Tong-Min (Department of Environmental and Biological Chemistry, Chungbuk National University)
  • Received : 2012.01.06
  • Accepted : 2012.01.27
  • Published : 2012.02.29

Abstract

Salinity is one of the most relevant abiotic factor limiting crop yield and its net primary productivity. In addition, salinity induces an increased stress ethylene synthesis in plants which, in turn, exacerbate the responses to the stressor. Bacterial single or co-inoculation effect was tested using previously characterized plant growth promoting (PGP) bacteria Brevibacterium iodinum RS16 and Methylobacterium oryzae CBMB20 on maize and sorghum-sudan grass hybrid under different concentrations of NaCl. Non-inoculated maize and sorghum-sudangrass hybrid showed 33.4% and 20.0% reduction in seed germination under highest NaCl (150 mM) level tested. However, under the same NaCl concentration, co-inoculation with B. iodinum RS16 and M. oryzae CBMB20 PGP strains increased the seed germination in maize (16.7%) and sorghum-sudangrass hybrid (4.4%). In Gnotobiotic growth pouch experiments conducted for maize and sorghum-sudangrass hybrid, co-inoculation of PGP B. iodinum RS16 and M. oryzae CBMB20 mitigated the salinity stress and promoted root length by 22.9% and 29.7%, respectively. Thus the results of this study could help in development of potential bioinoculants that may be suitable for crop production under saline conditions.

Acknowledgement

Supported by : Korea Institute of Planning & Evaluation for Technology in Food, Agriculture, Forestry and Fisheries (iPET)

References

  1. Ahmad, F., I. Ahmad, and M.S. Khan. 2006. Screening of Free-living Rhizospheric Bacteria for their Multiple Plant Growth Promoting activities. Microb. Res. 36:1-9.
  2. Almodares, A., M.R. Hadi, and B. Dosti. 2007. Effects of salt stress on Germination Percentage and Seedling Growth in Sweet Sorghum Cultivars. J. Biological Sci. 7(8):1492-1495. https://doi.org/10.3923/jbs.2007.1492.1495
  3. Bacilio, M., H. Rodriguez, and M. Moreno. 2004. Mitigation of Salt stress in Wheat seedlings by gfp-tagged Azospirillum lipoferum. Biol Fertil soils. 40:188-193.
  4. Belimov, A.A., N. Hontzeas, and V.I. Safronova. 2005. Cadmium-tolerant Plant Growth Promoting Bacteria Associated with the Roots of Indian mustard. Soil Biol Biochem. 37:241-250. https://doi.org/10.1016/j.soilbio.2004.07.033
  5. Bharathi, R., R. Vivekananthan, S. Harish, A. Ramanathan, and R. Samiyappan. 2004. Rhizobacteria-based Bio-formulations for the Management of Fruit rot infection in chillies. Crop Protec. 23:835-843. https://doi.org/10.1016/j.cropro.2004.01.007
  6. Blanco, F.F., M.V. Folegatti, H.R. Gheyi, and P.D. Fernandes. 2007. Emergence and Growth of Corn and Soybean Under Saline Stress. Sci. Agric (Piracicaba, Braz.). 64(5):451-459.
  7. Blum, A. 1985. Breeding for Crop Varieties for Stress Environments Crit. Rev. Plant Sci. 2:199-238. https://doi.org/10.1080/07352688509382196
  8. Bray, E.A., J. Bailey-Serres and E. Weretilnyk. 2000. Responses to abiotic stress. Biochemistry and Molecular Biology of Plants. American Society of Plant Physiology. Rockville. 1158-1203.
  9. Bybordi, A., and S.J. Tabatabaei. 2009. Effect of Salinity Stress on Germination and Seedling Properties in Canola Cultivars (Brassica napus L). Notulae Botanicae Horti Agrobotanici Cluj-Napoca. 37(1):71-76.
  10. Cattelan, A.J., P.G. Hartel, and J.J. Fuhrmann. 1999. Screening for Plant Growth Promoting Rhizobacteria to Promote Early soybean growth. Soil Sci. Soc. Am. J. 63:1670-1680. https://doi.org/10.2136/sssaj1999.6361670x
  11. Cuartero, J., M.C. Bolarin, M.J. Asins, and V. Moreno. 2006. Increasing Salt Tolerance in the Tomato. J. Exp. Bot. 57(5):1045-1058.
  12. Egamberdiyeva, D. 2007. The Effect of Plant Growth Promoting Bacteria on Growth and Nutrient Uptake of Maize in Two Different Soils. Appl. Soil. Eco. 36:184-189. https://doi.org/10.1016/j.apsoil.2007.02.005
  13. Egamberdiyeva, D. 2009. Alleviation of Salt Stress by Plant Growth Regulators and IAA Producing Bacteria in wheat. Acta Physiol Plant. 31:861-864. https://doi.org/10.1007/s11738-009-0297-0
  14. Ghosh, S., J.N. Penterman, and R.D. Little. 2003. Three Newly Isolated Plant Growth Promoting Bacilli Facilitate The Seedling Growth Of Canola, Brassica Campestris. Plant Physiol Biochem. 41:277-281. https://doi.org/10.1016/S0981-9428(03)00019-6
  15. Gill, P.K., A.D. Sharma, P. Singh, and S.S. Bhullar. 2002. Osmotic Stres-Incuded Changes in Germination, Growth and Soluble Sugar Changes of Sorghum bicolor (L.) Seeds. Bulg. J. Plant Physiol. 28:12-25.
  16. Glick, B.R., C. Liu, and S. Ghosh. 1997. Early Development of Canola Seedlings in the Presence of the Plant Growth Promoting Rhizobacterium Pseudomonas putida GR12-2, Soil Biol Biochem. 29:1233-1239. https://doi.org/10.1016/S0038-0717(97)00026-6
  17. Glick, B.R., D.M. Penrose, and J. Li. 1998. A Model for the Lowering of Plant Ethylene Concentrations by Plant Growth Promoting Bacteria, J Theor Biol. 190:63-68. https://doi.org/10.1006/jtbi.1997.0532
  18. Grichko, V.P., and B.R. Glick. 2001. Amelioration of Flooding Stress by ACC deaminase-containing Plant Growth Promoting Bacteria. Plant Physiol Biochem. 39:11-17. https://doi.org/10.1016/S0981-9428(00)01212-2
  19. Jackson, M.B. 1997. Hormones from Roots as Signal for the Shoots of Stressed Plants. Trends Plant Sci. 2:22-28 64. https://doi.org/10.1016/S1360-1385(96)10050-9
  20. Jeun, Y.C., K.S. Park, C.H. Kim, W.D. Fowler and J.W. Kloepper. 2004. Cytological Observations of Cucumber Plants During Induced Resistance Elicited by Rhizobacteria. Biol. Contorl. 29:34-42. https://doi.org/10.1016/S1049-9644(03)00082-3
  21. Khan, M.A., and I.A. Ungar. 1985. The Role of Hormones in Regulating the Germination of Polymorphic Seeds and Early Seedling Growth of Atriplex triangularis Willd. under saline conditions. Physiol. Plant. 63:109-113. https://doi.org/10.1111/j.1399-3054.1985.tb02827.x
  22. Kokelis-Burelle, N., J.W. Kloepper and M.S. Reddy. 2006. Plant Growth Promoting Rhizobacteria as Transplant Amendments and their Effects on Indigenous Rhizosphere Microorganisms. App. Soil Ecol. 31:91-100.
  23. Madhaiyan, M., B.Y. Kim, S. Poonguzhali, S.W. Kwon, M.H. Song, J.H. Ryu, S.J. Go, B.S. Koo, and T.M. Sa. 2007. Methylobacterium oryzae spp. nov., a Novel Aerobic, Pinkpigmented, Facultatively Methylothropic, 1-aminocyclo-propan-1-carboxylate Deaminase Producing Bacterium Isolated from Rice. Int. J. Syst, Evol. Microbiol. 57:326-331. https://doi.org/10.1099/ijs.0.64603-0
  24. Mayak, S., T. Tirosh, and B.R. Glick. 2004. Plant Growth Pomoting Bacteria Confer Resistance in Tomato Plants to Salt Stress. Plant Physiol Biochem. 42:565-572. https://doi.org/10.1016/j.plaphy.2004.05.009
  25. Mohammad, M.R., M.A., Shibli and L. Nimri. 1998. Tomato Root and Shoot Responses to Salt Stress Under Different levels of Phosphorus Nutrition. Journal of plant nutrition. 21(8):1667-1680. https://doi.org/10.1080/01904169809365512
  26. Munns, R. 2002. Comparative Physiology of Salt and Water Stress. Plant Cell Environ. 25:239-250. https://doi.org/10.1046/j.0016-8025.2001.00808.x
  27. Nadeem, S.M., A. Zahir, M. Zahir, M. Naveed, Arshad, and S.M. Shahzad. 2006. Variation in Growth and Ion uptake of Maize due to Inoculation with Plant Growth Promoting Rhizobacteria under Salt Stress. Soil & Environ. 25(2):78-84.
  28. Nelson, L.M. 2004. Plant growth promoting rhizobacteria (PGPR): Prospects for New Inoculants. Online. Crop Management doi: 10.1094/CM-2004- 0301-05-RV.
  29. Neumann, P.M. 1995. Inhibition of Root Growth by Salinity Stress. In: Structure and Function of Roots. Baluska F, Ciamporova M, Gasporikova O, Barlow PW (Eds.). Kluwer Academic Publishers, Netherlands. 299-304.
  30. Rendig, V.V., and H.M. Taylor. 1989. Principles of Soil-plant Interrelationships. McGraw-Hill, New York, NY.
  31. Salantur, A., A. Ozturk, and S. Akten. 2006. Growth and Yield Response of Spring Wheat (Triticum aestivum L.) to Inoculation with Rhizobacteria. Plant. Soil. Environ. 52(3):111-118.
  32. Saritha, V., and M.N.V. Kuriakose-Prasad. 2007. Cadmium Stress Affects seed Germination and Seedling growth in Sorghum bicolor (L.) Moench by Changing the Activities of Hydrolyzing Enzymes. Plant Growth Regul. 54:43-156.
  33. Shaharoona, B., M. Arshad, and Z.A. Zahir. 2006. Effect of Plant Growth Promoting Rhizobacteria Containing ACC-deaminase on Maize (Zea mays L.) Growth under Axenic Conditions and on Nodulation in Mung bean (Vigna radiata L.). Lett Appl Microbiol, 42:155-159. https://doi.org/10.1111/j.1472-765X.2005.01827.x
  34. Siddikee, Md.A., P.S. Chauhan, T.M. Sa. 2011a. Regulation of Ethylene Biosynthesis Under Salt Stress in Red Pepper (Capsicum annuum L.) by 1-Aminocyclopropane-1-Carboxylic Acid (ACC) Deaminase producing Halotolerant Bacteria. J Plant Growth Regul. DOI 10.1007/s00344-011-9236-6.
  35. Siddikee, Md.A., R. Bernard, B.R. Glick, P.S. Chauhan, W.J. Yim, and T.M. Sa. 2011b. Enhancement of Growth and Salt Tolerance of Red pepper seedlings (Capsicum annuum L.) by Egulating Stress Ethylene Synthesis with Halotolerant Bacteria Containing 1-aminocyclo- propane-1-carboxylic acid deaminase activity. Plant Physiology and Biochemistry, 49:427-434. https://doi.org/10.1016/j.plaphy.2011.01.015
  36. Simon, E.W. 1984. Early Events in Germination. In: Murray DR, ed. Seed physiology. Australia: Academic Press.
  37. Taffouo, V.D., M. Kenne, O. Wamba-Fotsop, M.L. Sameza, M. Ndomou and A. Amougou. 2006. Salinity Effects on Growth, Ionic Distribution and Water Content in Salt-tolerant Species Gossypium hirsutum (Malvaceae). J. Cam. Acad. Sci. 6:167-174.
  38. Uhvits, R. 1946. Effect of Osmotic Pressure on Water Absorption and Germination of Alfalfa seeds. American Journal of Botany. 33:278-284. https://doi.org/10.2307/2437434
  39. Werner, J.E, and R.R. Finkelstein. 1995. Arabidopsis Mutants with Reduced Response to NaCl and Osmotic Stress. Physiologia Plantarum. 93:659-666. https://doi.org/10.1111/j.1399-3054.1995.tb05114.x
  40. Wu S.C., Z.H. Cao, Z.G. Li, K.C. Cheung, and M.H. Wong. 2005. Effects of Biofertilizer Containing N-fixer, P and K solubilizers and AM fungi on Maize Growth: a greenhouse trial. Geoderma. 125:155-166. https://doi.org/10.1016/j.geoderma.2004.07.003
  41. Zadeh, H.M. and M.B. Naeni. 2007. Effects of Salinity Stress on the Morphology and Yield of Two Cultivars of Canola (Brassica napus L.). J. Agron. 6:409-414. https://doi.org/10.3923/ja.2007.409.414
  42. Zholkevich, V.N. and T.N. Pustovoytova. 1993. The Role of Cucumis sativum L Leaves and Content of Phytohormones under Soil Drought. Russ. J. Plant Physiol. 40:676-680.

Cited by

  1. Tolerance to Salt Stress by Plant Growth-Promoting Rhizobacteria on Brassica rapa var. glabra vol.49, pp.6, 2016, https://doi.org/10.7745/KJSSF.2016.49.6.776
  2. Effect of co-inoculation of Brevibacterium iodinum RS16 and Methylobacterium oryzae CBMB20 on the early growth of crop plants in Saemangeum reclaimed soil vol.47, pp.1, 2014, https://doi.org/10.7745/KJSSF.2014.47.1.001
  3. Physiological and biochemical perspectives of non-salt tolerant plants during bacterial interaction against soil salinity vol.116, 2017, https://doi.org/10.1016/j.plaphy.2017.05.009