• Title/Summary/Keyword: sucrose.

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Simultaneous Formation of Fructosyltransferase and Glucosyltransferase in Aureobasidium pullulans

  • Yun, Jong-Won;Kim, Dong-Hyun;Moon, Hye-Yeon;Song, ChiiI-Hyun;Song, Seung-Koo
    • Journal of Microbiology and Biotechnology
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    • v.7 no.3
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    • pp.204-208
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    • 1997
  • Aureobasidium puliulans possesses the capacity for simultaneous formation of fructosyltransferase and glucosyltransferase in various sugar media including sucrose, maltose, glucose and fructose. Among them, sucrose (300 g/1) was the most suitable carbon source for fructosyltransferase production, while fructose (100 g/1) gave the maximal production of glucosyltransferase. There existed a critical concentration for the optimal formation of enzymes in sucrose, glucose and fructose media. By contrast, no effect of maltose concentrations up to 300 g/1 was observed. The specific activity of the glucosyltransferase on maltose medium was highest during the early period of fetmentation, after which a sharp decrease occurred, whereas fructosyltransferase activity on sucrose medium maintained a nearly constant rate for a given culture period. Concomitant production of fructosyltransferase and glucosyltransferase was investigated with different combinations of lower concentrations of sucrose and maltose. Maltose supplementation in sucrose media and sucrose addition to maltose media enhanced the activity ratios of fructosyltransferase to glucosyltransferase as compared to that of non-supplemented media. Several polymers and surfactants were added in an attempt to enhance enzyme production, and supplementation of polyoxyethylene-sorbitan monolaurate (Tween 20) promoted fructosyltransferase production by 20%.

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Pain Relieving Effect of Sucrose Coating Pacifier in Neonates (신생아 통증완화를 위한 자당 코팅 노리개 젖꼭지의 적용 효과)

  • Chung Yoon-Chung;Cho Kyoul-Ja
    • Child Health Nursing Research
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    • v.11 no.3
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    • pp.356-364
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    • 2005
  • Purpose: This study is to assess and compare the analgegic effects of $25\%$ sucrose coating pacifier and pacifier. Method: The participants are 75 healthy infants of neonatal age 1-7 days and randomized to receive heel prick before 2 minutes to blood sampling and physiological test in Nursery of A Medical Center from 24. January to 28, February, 2003. The experimental group assigned to one of three treatment groups: no treatment, a pacifier, $25\%$ sucrose coating pacifier. Collected data were analyzed with the SPSS 11.0 program using $x^2$-test, one-way ANOVA and Scheffe, repeated mesured ANOVA. Results: The pain score of $25\%$ sucrose coating pacifier is lower than no treatment group and pacifier group. In heart rate, there were statistical significant differences between three groups. In repiratory rate, there were no statistical significant differences between three groups. In $SaO_2$, there were statistical significant difference between three groups. Conclusion: The $25\%$ sucrose coating pacifier showed pain relief effect in behavior responses and heart rate and $SaO_2$. Accordingly, the sucrose coating pacifier should be applied nursing intervention for simple pain management as heel prick.

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Regeneration of Bulblets from Bulblet-Derived Bulb-Scales of Lilium longiflorum. (백합 기내자구 유래 소인편배양에서 기관분화에 미치는 생장조절제 및 배지조성의 영향)

  • 이은모;정해준;이영복
    • Korean Journal of Plant Tissue Culture
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    • v.22 no.2
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    • pp.89-93
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    • 1995
  • Regeneration and growth of bulblets from bulblet-derived bulb-scale segment of Lilium longiflorum (cv Georgia) were investigated. Bulblets were initiated on bulb scales taken from bulblets on MS medium containing 0.05 mg/L 2,4D with 3% sucrose or 0.02 mg/L 2,4D with 9% sucrose. Benzyladenine promoted the differentiation of bulblets but inhibited the growth of differentiating bulblets. The growth of bulblet was promoted by supplying 1/2 strength 1/2 NH$_4$NO$_3$ concentration in MS medium containing 12% sucrose.

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Effect of Sucrose Stearate on the Sensory-Related Quality of the Broth and Porridge of Ready-To-Eat Ginseng Chicken Soup Samgyetang

  • Triyannanto, Endy;Lee, Keun Taik
    • Food Science of Animal Resources
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    • v.37 no.6
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    • pp.855-864
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    • 2017
  • The objective of this study was to assess the sensory-related characteristics of the broth and porridge of ready-to-eat (RTE) ginseng chicken soup (Samgyetang) with sucrose stearate added at various concentrations (0.1%, 0.2%, and 0.3%) during storage at $25^{\circ}C$ for 12 mon. Scores indicating the lightness and size of fat droplets in the broth increased during storage as the sucrose stearate concentration increased, while the clarity scores decreased until 9 mon and the taste scores decreased throughout the storage period (p<0.05). The porridge lightness increased as the concentration of sucrose stearate increased after 6 mon (p<0.05), while scores indicating the softness and vividness were higher for treated samples with sucrose stearate than for the control group after 3 mon, despite a lack of significant differences among treatment groups (p>0.05). The taste scores were lower for treated porridge samples than for the control group (p<0.05), even though no significant differences were observed among the treatment groups (p>0.05). The addition of sucrose stearate to the RTE Samgyetang broth improved the lightness (CIE $L^*$) value of the broth and various sensory palatability parameters, including the color and fat droplet size of the broth and the softness and vividness of the porridge, despite reductions in broth clarity and taste scores for the broth and porridge during storage.

Effects of sucrose and MS salts in the addition of liquid medium on in vitro bulblet growth of Lilium oriental hybrid 'Casa Blanca' (액체배지 첨가시 sucrose 및 MS 염류가 Lilium Oriental Hybrid 'Casa Blanca' 의 기내 자구비대에 미치는 영향)

  • 한봉희;예병우;백기엽;염미란
    • Korean Journal of Plant Tissue Culture
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    • v.28 no.5
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    • pp.239-242
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    • 2001
  • The effects of post-cultural addition of liquid medium addition to stimulate in vitro bulblet growth in Lilium oriental hybrid 'Casa Blanca' were investigated. The sections of bulblets with swollen basal plate (7 mm$\times$12 mm) were cultured on medium containing 60 g/L sucrose and 1 g/L activated charcoal for two months in dark, and then, liquid medium was added into the same vessels. The addition of liquid medium stimulated the growth of bulblets remarkably, compared to no addition of liquid medium. The liquid medium supplemented with 120 g/L sucrose and double strength of MS salts were the most effective on the growth of in vitro bulblets.

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Structure Determination of Sucrose by Acetylation and Acid Hydrolysis

  • Min, Hee-Jeong;Lee, Tae-Seong;Bae, Young-Soo
    • Journal of the Korean Wood Science and Technology
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    • v.42 no.2
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    • pp.183-192
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    • 2014
  • For the structure determination of D-(+)-sucrose, which consists of ${\alpha}$-D-(+)-glucose and ${\beta}$-D-(+)-fructose, it was acetylated with acetic anhydride and triethyl amine, pyridine, zinc chloride, and sodium acetate as catalysts. The acetylated D-(+)-sucrose was acid-hydrolyzed using sulfuric acid and sodium chloride in methanolic solution. The structures of the reaction products were determined by $^1H$-NMR and $^{13}C$-NMR spectra. The yield of the acetylation indicated the high value in zinc chloride as 70% in zinc chloride catalyst. The acid-hydrolyzed product of the acetylated D-(+)-sucrose, 2,3,4,6,1',3',4',6'-octa-O-acetyl-D-(+)-sucrose, gave 2,3,4,6-tetra-O-acetyl-${\beta}$-D-(+)-glucose and it suggests that the acetylated D-(+)-sucrose was rearranged through the formation of oxonium ion by mutarotation in the 2,3,4,6-tetra-O-acetyl-${\alpha}$-D-(+)-glucose moiety and through the ring opening in the 1',3',4',6'-tetra-O-acetyl-${\beta}$-D-(+)-fructose moiety.

Effect of pH, Temperature, and added Sucrose on the Production of Vitamin $B_{12}$ and Riboflavin by Bacillus megaterium and Enterobacter aerogenes (온도, pH 및 첨가된 Sucrose가 Bacillus megaterium 과 Enterobacter aerogenes 에 의한 비타민 $B_{12}$ 와 Riboflavin 생산에 미치는 영향)

  • Chung, Hee-Jong;Marion L. Fields
    • Microbiology and Biotechnology Letters
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    • v.15 no.2
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    • pp.112-115
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    • 1987
  • Optimal pH temperature and sucrose content for the production of vitamin B$_{12}$ and riboflavin by Bacillus megaterium and Enterobacter aerogenes was studied by microbiological analysis. Optimal pH for the production of B$_{12}$ was 6.0 by B. megaterium while the pH for E. aerogenes was 5.0. However, upon the addition of sucrose the optimal pH for B. megaterium shifted to 7.5 but E. aerogenes remained at pH 5.0. In the absence of sucrose, pH did not influence the yields of riboflavin produced by either bacterium. Addition of sucrose stimulated synthesis of riboflavin by both bacteria. Temperature had little effect on the production of vitamins by either bacterium.

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Analysis of the Separation Using Disc Plate and Frame Type Reverse Osmosis Module (원판틀형 역삼투 모듈을 이용한 분리 특성 해석)

  • Chung, Kun-Yong;Chung, Wook;Won, Jang-Mook;Bae, Seong-Youl;Ha, Baik-Hyon
    • Membrane Journal
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    • v.5 no.2
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    • pp.81-88
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    • 1995
  • Separation performance was measured for the disc plate and frame type reverse osmosis module using NaCl and sucrose solutions. An analysis of membrane performance was done following the equations proposed by Kimura-Sourirajan[8]. The membrane permeability was $2.17 \times 10^{-6}$(gmol/$cm^2$-sec-bar) and independent of operating pressure. The effect of concentration polarization for sucrose solution was higher than that of NaCl. Permeation flux for sucrose solution above 40 bar was decreased as operating pressure was increased. Solute rejection for NaCl solution was decreased, but that of sucrose was increased as operating pressure was increased.

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Changes of Chemical Components during Storage in Sweet Potato Produced at Two Locations (산지가 다른 고구마 품종의 저장중 화학성분 변화)

  • Oh, Sung-Kun;Kim, Deog-Su;Chin, Moon-Sup;Seong, Rak-Chun
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.39 no.1
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    • pp.85-91
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    • 1994
  • This experiment was conducted to obtain the basic information of the major chemical components for breeding high quality varieties of sweet potatoes [Ipomoea batatas (L) Lamk]. Six recommended varieties were cultivated at the experimental plots of Hwasung and Suwon in 1992. Starch value. glucose, fructose, maltose, and sucrose content were analyzed with time intervals during storage period. Starch value of Shinyulmi was the highest among six varieties in both locations. Glucose and fructose contents were appeared wide variations in varieties and locations. Shinyulmi showed the highest maltose content and Yulmi had the highest sucrose content in two locations. Total sugar contents were higher in Shinyulmi and Yulmi. Starch value, glucose, fructose, maltose, and sucrose content were stable during storage period, however these were differed between locations.

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Establishment of a New Herbicides Screening Method Using Photoautotrophic Cultured Cell I. Studies on Culture Conditions for Developing Photoautotrophic Cells in Tobacco (광독립영양세포(光獨立營養細胞)를 이용(利用)한 새로운 제초제(除草劑) 선발법(選拔法) 확립(確立) I. 담배의 광독립영양세포(光獨立營養細胞) 육성(育成)을 위한 배양조건(培養條件)의 구명(究明))

  • Kim, K.U.;Suh, S.K.;Kwon, S.T.
    • Korean Journal of Weed Science
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    • v.11 no.1
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    • pp.68-73
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    • 1991
  • This study was conducted to determine the appropriate culture conditions for developing photoautotrophic cells from tobacco using plant growth regulators, sucrose and light condition in LS medium. The greatest callus induction was observed in the LS medium supplemented with $10^{-5}$ M NAA and $10^{-4}$ M BA, showing 3.08 g of callus fresh weight determined at 30 days after incubation. The highest chlorophyll content of callus was obtained in the LS medium supplemented with $10^{-5}$M NAA and $10^{-6}$ M BA, showing 28.42${\mu}g/g$. In both light and dark conditions, callus induction increased as the concentration of sucrose increased from 0.5%to 3.0%. In particular, in the light condition, the greatest callus induction was made in the LS medium supplemented with 2% sucrose. On the other hand, the heighest chlorophyll content was observed at 0.5% sucrose and chlorophyll wasn't induced in dark condition. The chlorophyll content of callus cultured in LS medium containing 0.75% sucrose was similar to 1% sucrose, as far as the chlorophyll content of callus was concerned.

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