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Overexpression of a Rice Diacylglycerol Kinase Gene OsBIDK1 Enhances Disease Resistance in Transgenic Tobacco

  • Zhang, Weidong;Chen, Jie;Zhang, Huijuan;Song, Fengming
    • Molecules and Cells
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    • v.26 no.3
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    • pp.258-264
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    • 2008
  • A rice diacylglycerol kinase (DGK) gene, OsBIDK1, which encodes a 499-amino acid protein, was cloned and characterized. OsBIDK1 contains a conserved DGK domain, consisting of a diacylglycerol kinase catalytic subdomain and a diacylglycerol kinase accessory subdomain. Expression of OsBIDK1 in rice seedlings was induced by treatment with benzothiadiazole (BTH), a chemical activator of the plant defense response, and by infection with Magnaporthe grisea, causal agent of blast disease. In BTH-treated rice seedlings, expression of OsBIDK1 was induced earlier and at a higher level than in water-treated control seedlings after inoculation with M. grisea. Transgenic tobacco plants that constitutively express the OsBIDK1 gene were generated and disease resistance assays showed that overexpression of OsBIDK1 in transgenic tobacco plants resulted in enhanced resistance against infection by tobacco mosaic virus and Phytophthora parasitica var. nicotianae. These results suggest that OsBIDK1 may play a role in disease resistance responses.

Reliability and Validity of the Korean Version of the Copenhagen Psyco-social Questionnaire Scale (한국어판 코펜하겐 사회심리적 업무환경 측정도구의 타당도와 신뢰도)

  • June, Kyung Ja;Choi, Eun Suk
    • Korean Journal of Occupational Health Nursing
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    • v.22 no.1
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    • pp.1-12
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    • 2013
  • Purpose: This study aims to test the validity and criteria-related reliability of the Korean version of the Copenhagen Psyco-social Questionnaire version II (COPSOQ-K) assessing the psychosocial working environment. Methods: The COPSOQ-K was developed through forward-backward translation techniques, and revision based on feedback from focus groups. Survey data were collected from 311 office workers who worked in one workplace. An internal consistency reliability was estimated by Cronbach's ${\alpha}$. The impacts of the COPSOQ-K scales on job satisfaction, self-rated health, stress, sleeping troubles, burnout, and sickness absence were analyzed with multiple regression model or multiple logistic regression model, adjusted age and gender using SAS version 9.3. The Pearson correlation coefficients between the COPSOQ-K scales and Korean job stress and its subdomain were identified. Results: Cronbach's ${\alpha}$ of COPSOQ-K scales was adequate or good (0.66~0.87). The major COPSOQ-K scales predict job satisfaction, self-rated health, stress, sleeping troubles burnout, and sickness absence. The major COPSOQ-K scales were correlated with Korean job stress and its subdomain. Conclusion: The COPSOQ-K scales have satisfactory reliability and criteria-related validity. The COPSOQ-K scales will be useful for the future studies and practices associated with psychosocial working environment.

The Relationship of Self-Efficacy, Perceived Barriers and Health Promoting Behaviors in Stroke (뇌졸중 환자의 자기효능감, 지각된 장애성과 건강증진행위간의 관계)

  • Woo, Myung-E;Ku, In-Sun;Chun, Hee-Jung;Choi, Seung-Hee;Jung, Hye-Jin;Yu, Hae-Jung;Roh, Young-Sook
    • Journal of Home Health Care Nursing
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    • v.12 no.2
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    • pp.16-32
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    • 2005
  • Purpose: The purpose of this study was to: (1) describe the self-efficacy, perceived barriers and health promoting behaviors and (2) determine the relationship of these variables in stroke survivors. Methods: A convenience sample of 93 stroke survivors who were enrolled in a neurosurgical outpatient clinic in Seoul participated in the study. Results: Among health promoting behaviors, non-smoking was the subdomain with the highest mean score and exercise subdomain had the lowest mean score. Significant factors correlated with health promoting behaviors were age, self-efficacy and perceived barriers. Conclusion: Findings from this study suggest that health promotion programs aimed at enhancing self-efficacy and decreasing perceived barriers would be beneficial for the stroke survivors.

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Factors Influencing Job Satisfaction Among Nurses in Integrated Nursing Care Service Wards (간호·간병통합서비스 병동 간호사의 직무만족에 미치는 영향요인)

  • Kim, Jung-Mi;Lee, Yu-Mi
    • The Korean Journal of Health Service Management
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    • v.13 no.3
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    • pp.53-65
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    • 2019
  • Objectives: This descriptive survey study aimed to identify factors that affect job satisfaction among nurses working in integrated nursing care service wards and to explore measures to increase job satisfaction among these nurses. Methods: Data were collected between July 20 and August 3, 2018 from nurses working in integrated nursing care service wards in Busan and Changwon city, in Korea. Data were analyzed with SPSS/WIN 23.0 software(IBM corp., Armonk, NY, USA). Results: Factors affecting job satisfaction among nurses working in integrated nursing care service wards were identified as follows: professional self-concept (${\beta}=.32$, p=.003), which is a subdomain of nursing professionalism, and nursing foundations for quality care (${\beta}=.30$, p=.001), which is a subdomain of nursing work environment. The model had an explanatory power of 54.7%. Conclusions: Nursing professionalism should be strongly established among nurses, as nurses are key players in a health system that allows stable and expanded operations of integrated nursing care. Intervention measures that create a better work environment for nurses should also be implemented. Furthermore, it is necessary to develop systematic strategies and multifaceted efforts to increase nurses' job satisfaction in integrated nursing care service wards.

In silico Design of Discontinuous Peptides Representative of B and T-cell Epitopes from HER2-ECD as Potential Novel Cancer Peptide Vaccines

  • Manijeh, Mahdavi;Mehrnaz, Keyhanfar;Violaine, Moreau;Hassan, Mohabatkar;Abbas, Jafarian;Mohammad, Rabbani
    • Asian Pacific Journal of Cancer Prevention
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    • v.14 no.10
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    • pp.5973-5981
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    • 2013
  • At present, the most common cause of cancer-related death in women is breast cancer. In a large proportion of breast cancers, there is the overexpression of human epidermal growth factor receptor 2 (HER2). This receptor is a 185 KDa growth factor glycoprotein, also known as the first tumor-associated antigen for different types of breast cancers. Moreover, HER2 is an appropriate cell-surface specific antigen for passive immunotherapy, which relies on the repeated application of monoclonal antibodies that are transferred to the patient. However, vaccination is preferable because it would stimulate a patient's own immune system to actively respond to a disease. In the current study, several bioinformatics tools were used for designing synthetic peptide vaccines. PEPOP was used to predict peptides from HER2 ECD subdomain III in the form of discontinuous-continuous B-cell epitopes. Then, T-cell epitope prediction web servers MHCPred, SYFPEITHI, HLA peptide motif search, Propred, and SVMHC were used to identify class-I and II MHC peptides. In this way, PEPOP selected 12 discontinuous peptides from the 3D structure of the HER2 ECD subdomain III. Furthermore, T-cell epitope prediction analyses identified four peptides containing the segments 77 (384-391) and 99 (495-503) for both B and T-cell epitopes. This work is the only study to our knowledge focusing on design of in silico potential novel cancer peptide vaccines of the HER2 ECD subdomain III that contain epitopes for both B and T-cells. These findings based on bioinformatics analyses may be used in vaccine design and cancer therapy; saving time and minimizing the number of tests needed to select the best possible epitopes.

Linear and Conformational B Cell Epitope Prediction of the HER 2 ECD-Subdomain III by in silico Methods

  • Mahdavi, Manijeh;Mohabatkar, Hassan;Keyhanfar, Mehrnaz;Dehkordi, Abbas Jafarian;Rabbani, Mohammad
    • Asian Pacific Journal of Cancer Prevention
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    • v.13 no.7
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    • pp.3053-3059
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    • 2012
  • Human epidermal growth factor receptor 2 (HER2) is a member of the epidermal growth factor receptor family of receptor tyrosine kinases that plays important roles in all processes of cell development. Their overexpression is related to many cancers, including examples in the breast, ovaries and stomach. Anticancer therapies targeting the HER2 receptor have shown promise, and monoclonal antibodies against subdomains II and IV of the HER2 extra-cellular domain (ECD), Pertuzumab and Herceptin, are currently used in treatments for some types of breast cancers. Since anti HER2 antibodies targeting distinct epitopes have different biological effects on cancer cells; in this research linear and conformational B cell epitopes of HER2 ECD, subdomain III, were identified by bioinformatics analyses using a combination of linear B cell epitope prediction web servers such as ABCpred, BCPREDs, Bepired, Bcepred and Elliprro. Then, Discotope, CBtope and SUPERFICIAL software tools were employed for conformational B cell epitope prediction. In contrast to previously reported epitopes of HER2 ECD we predicted conformational B cell epitopes $P1_C$: 378-393 (PESFDGDPASNTAPLQ) and $P2_C$: 500-510 (PEDECVGEGLA) by the integrated strategy and P4: PESFDGD-X-TAPLQ; P5: PESFDGDP X TAPLQ; P6: ESFDGDP X NTAPLQP; P7: PESFDGDP-X-NTAPLQ; P8: ESFDG-XX-TAPLQPEQL and P9: ESFDGDP-X-NTAPLQP by SUPERFICIAL software. These epitopes could be further used as peptide antigens to actively immune mice for development of new monoclonal antibodies and peptide cancer vaccines that target different epitopes or structural domains of HER2 ECD.

Nonlinear Finite Element-Boundary Element Analysis of Multi-Layered Structural Systems (유한요소와 경계요소의 조합에 의한 다층 구조계의 비선형 해석)

  • 김문겸;허택녕;이상도
    • Computational Structural Engineering
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    • v.7 no.4
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    • pp.57-67
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    • 1994
  • It is usual that underground structures are constructed within a multi-layered medium. In this paper, an efficient numerical modelling of multi-layered structural systems is studied using coupled analysis of finite elements and boundary elements. The finite elements are applied to the area in which the material nonlinearity dominates, and the boundary elements are applied to the far field where the nonlinearity is relatively weak. In the boundary element modelling of the multi-layered medium, fundamental solutions are not readily available. Thus, methods which can utilize existing Kelvin solutions are sought for the interior multi-layered domain problem. The interior domain problem which has piecewise homogeneous layers is analyzed using boundary elements with Kelvin solution, by discretizing each homogeneous subdomain and enforcing compatibility and equilibrium conditions between interfaces. Developed methodology is verified by comparing its results with those from the finite element analysis and it is concluded that coupled analysis using boundary elements and finite elements can be reasonable and efficient.

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A Study on the Environmental Awareness and Attitude of Elementary School Students (초등학교 학생들의 환경인식과 태도에 관한 연구)

  • 김인호;주신하;안동만
    • Hwankyungkyoyuk
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    • v.13 no.1
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    • pp.122-132
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    • 2000
  • The purpose of this study is to survey and analyze the environmental awareness and attitude of elementary school students, adapting the CHEAKS(Children's Environmental Attitude and Knowledge Scale) for Korean elementary students, which is the valid-tested tools for children's environmental awareness and altitude. The subjects are 580 students from 6 elementary schools. The differences of the environmental attitude are surveyed and analyzed by environmental education activities, such as environmental activities in school and “the school forest movement”, and environmental awareness and concern. The results are as followings: First, the subjects replied that the environmental problems are very serious, such as atmospheric pollution(37.6%), water pollution(22.2%) and food waste(16.3%), but they are relatively optimistic of the future environment. Second, the verbal commitment subdomain and the affect domain are evaluated higher than the actual commitment subdomain, and the environmental issues of water, energy and recycling are considered more importantly than other issues such as environmental generals, pollutions and animals. Third, the grade and the sex have little effect on the environmental attitude, but the environmental education activities have great positive effect, such as the experience of environmental camping, the education of saving resources, waste separation and the experience of raising plants and flowers. Fourth, the students with high awareness and concern about the environmental problems have high environmental attitude. Especially, the awareness and concern on the flowers and plants have very strong correlation with environmental attitude, regardless of the subdomains. Finally, the students from the school participating “the school forest movement” have relatively positive environmental attitude. Particularly, the participation of students and the positive approach of the school have positively great influence on the students' environmental attitude in all of 3 subdomains and 6 environmental issues.

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Computerized Sunnybrook facial grading scale (SBface) application for facial paralysis evaluation

  • Jirawatnotai, Supasid;Jomkoh, Pojanan;Voravitvet, Tsz Yin;Tirakotai, Wuttipong;Somboonsap, Natthawut
    • Archives of Plastic Surgery
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    • v.48 no.3
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    • pp.269-277
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    • 2021
  • Background The Sunnybrook facial grading scale is a comprehensive scale for the evaluation of facial paralysis patients. Its results greatly depend on subjective input. This study aimed to develop and validate an automated Sunnybrook facial grading scale (SBface) to more objectively assess disfigurement due to facial paralysis. Methods An application compatible with iOS version 11.0 and up was developed. The software automatically detected facial features in standardized photographs and generated scores following the Sunnybrook facial grading scale. Photographic data from 30 unilateral facial paralysis patients were randomly sampled for validation. Intrarater reliability was tested by conducting two identical tests at a 2-week interval. Interrater reliability was tested between the software and three facial nerve clinicians. Results A beta version of the SBface application was tested. Intrarater reliability showed excellent congruence between the two tests. Moderate to strong positive correlations were found between the software and an otolaryngologist, including the total scores of the three individual software domains and composite scores. However, 74.4% (29/39) of the subdomain items showed low to zero correlation with the human raters (κ<0.2). The correlations between the human raters showed good congruence for most of the total and composite scores, with 10.3% (4/39) of the subdomain items failing to correspond (κ<0.2). Conclusions The SBface application is efficient and accurate for evaluating the degree of facial paralysis based on the Sunnybrook facial grading scale. However, correlations of the software-derived results with those of human raters are limited by the software algorithm and the raters' inconsistency.

The longitudinal effects of children's temperament on maternal depression: A hierarchical linear modeling approach

  • Kim, Chul-Gyu;Choi, Mi-Young
    • The Journal of Korean Academic Society of Nursing Education
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    • v.28 no.1
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    • pp.91-100
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    • 2022
  • Purpose: This study aimed to identify the longitudinal effects of children's temperament on maternal depression. Methods: Data from a longitudinal cohort of the Panel Study of Korean Children (PSKC) from 2010 to 2012 were analyzed using hierarchical linear modeling. The survey included 1,721 mother-child dyads. The mothers reported on their children's temperament and on maternal depression. The children's temperament was measured by the Emotionality, Activity and Sociability-Temperament Survey for Children-Parental Ratings, while maternal depression was measured by the Kessler 6 Psychological Distress Scale. Results: The results showed that both children's temperament and maternal depression were relatively stable when the children were between the ages of 2 to 4. The mean maternal depression scores were 11.83 in 2010, 11.88 in 2011, and 11.75 in 2012. There were significant negative correlations between the maternal depression scores and children's ages, and sociability and activity subdomain scores ranged from r=-.05 to -.11 (p<.05). There was a significant positive correlation between children's emotionality subdomain scores and maternal depression scores (r=.35, p<.001). Children's temperament rament (emotionality: β=0.26, activity: β=-0.07, and sociability: β=-0.03) were significant factors in maternal depression. Conclusion: These findings indicate the need for the early assessment of and intervention for children's temperament and maternal depression. The results of this study will provide basic data for the development of nursing education programs related to early assessment and intervention to improve the health and quality of life of young children and mothers.