Molecules and Cells

Korean Society for Molecular and Cellular Biology (한국분자세포생물학회)
권호 표지

Overexpression of a Rice Diacylglycerol Kinase Gene OsBIDK1 Enhances Disease Resistance in Transgenic Tobacco

  • Zhang, Weidong (State Key Laboratory of Rice Biology, Institute of Biotechnology, Zhejiang University at Huajiachi Campus) ;
  • Chen, Jie (State Key Laboratory of Rice Biology, Institute of Biotechnology, Zhejiang University at Huajiachi Campus) ;
  • Zhang, Huijuan (State Key Laboratory of Rice Biology, Institute of Biotechnology, Zhejiang University at Huajiachi Campus) ;
  • Song, Fengming (State Key Laboratory of Rice Biology, Institute of Biotechnology, Zhejiang University at Huajiachi Campus)
  • Received : 2008.02.01
  • Accepted : 2008.04.24
  • Published : 2008.09.30
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Abstract

A rice diacylglycerol kinase (DGK) gene, OsBIDK1, which encodes a 499-amino acid protein, was cloned and characterized. OsBIDK1 contains a conserved DGK domain, consisting of a diacylglycerol kinase catalytic subdomain and a diacylglycerol kinase accessory subdomain. Expression of OsBIDK1 in rice seedlings was induced by treatment with benzothiadiazole (BTH), a chemical activator of the plant defense response, and by infection with Magnaporthe grisea, causal agent of blast disease. In BTH-treated rice seedlings, expression of OsBIDK1 was induced earlier and at a higher level than in water-treated control seedlings after inoculation with M. grisea. Transgenic tobacco plants that constitutively express the OsBIDK1 gene were generated and disease resistance assays showed that overexpression of OsBIDK1 in transgenic tobacco plants resulted in enhanced resistance against infection by tobacco mosaic virus and Phytophthora parasitica var. nicotianae. These results suggest that OsBIDK1 may play a role in disease resistance responses.

Keywords

Acknowledgement

Supported by : National Natural Science Foundation of China

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